New publication: Novel adverse outcome pathways revealed by chemical genetics in a developing marine fish

Elin Sorhus§,*, John Patrick Incardona#, Tomasz Furmanek§, Giles W. Goetz#, Nathaniel L. Scholz#, Sonnich Meier§, Rolf Brudvik Edvardsen§,1, and Sissel Jentoft*,£,1 in eLIFE. Open access.

Abstract

Crude oil spills are a worldwide ocean conservation threat. Fish are particularly vulnerable to the oiling of spawning habitats, and crude oil causes severe abnormalities in embryos and larvae. However, the underlying mechanisms for these developmental defects are not well understood. Here we explore the transcriptional basis for four discrete crude oil injury phenotypes in the early life stages of the commercially important Atlantic haddock (Melanogrammus aeglefinus). These include defects in 1) cardiac form and function, 2) craniofacial development, 3) ionoregulation and fluid balance, and 4) cholesterol synthesis and homeostasis. Our findings suggest a key role for intracellular calcium cycling and excitation-transcription coupling in the dysregulation of heart and jaw morphogenesis. Moreover, the disruption of ionoregulatory pathways sheds new light on buoyancy control in marine fish embryos. Overall, our chemical-genetic approach identifies initiating events for distinct adverse outcome pathways and novel roles for individual genes in fundamental developmental processes.


Published 24 Jan 2017
DOI: 10.7554/eLife.20707
Publication webpage.

§ Institute of Marine Research, P.O. Box 1870, Nordnes, 5817, Bergen, Norway
* Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biosciences, UiO
# Environmental and Fisheries Science Division, Northwest Fisheries Science Center, National Marine Fisheries 11 Service, NOAA, 2725 Montlake Blvd. E., Seattle, WA 98112
£ Department of Natural Sciences, University of Agder, 4604 Kristiansand, Norway
1 Shared senior researcher authorship. 

Published Jan. 30, 2017 11:22 AM - Last modified Jan. 30, 2017 12:18 PM