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Subramani, Saranya & Winther-Larsen, Hanne Cecilie
(2023).
Identification of Novel Vaccine Candidates against Piscirikettsiosis.
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Mertes, Verena; Saragliadis, Athanasios; Mascherin, E; Antenucci, Fabio; Bojesen, Anders Miki & Colquhoun, Duncan John
[Vis alle 8 forfattere av denne artikkelen]
(2023).
Characterization of bacterial extracellular vesicles A versatile tool for potential vaccine application in aquaculture.
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Winther-Larsen, Hanne Cecilie; Langlete, Petter & Krabberød, Anders Kristian
(2023).
Loading cargo and messenging by bacterial extracellular vesicles.
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Mertes, Verena; Saragliadis, Athanasios; Ciani, Elia; Colquhoun, Duncan John; Linke, Dirk & Winther-Larsen, Hanne Cecilie
(2023).
Characterization of extracellular membrane vesicles secreted from the Gram-negative bacterial fish pathogen Yersinia ruckeri .
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Urtubia, Areli Moe; Winther-Larsen, Hanne Cecilie & Økstad, Ole Andreas
(2023).
Novel principles of drug resistance in the WHO priority 1 pathogen Acinetobacter baumannii.
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Winther-Larsen, Hanne Cecilie
(2023).
Bacterial extracellular vesicles.
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Mertes, Verena; Saragliadis, Athanasios; Attenucci, Fabio; Wrobel, Agnieszka; Ciani, Elia & Linke, Dirk
[Vis alle 8 forfattere av denne artikkelen]
(2022).
Characterization of bacterial extracellular vesicles from a fish pathogen, their role in virulence and possible function as vaccine in aquaculture.
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Mertes, Verena; Saragliadis, Athanasios; Ciani, Elia; Linke, Dirk; Colquhoun, Duncan J & Winther-Larsen, Hanne Cecilie
(2021).
Characterization of membrane vesicles from fish pathogens, their role in virulence and possible function as a vaccine in aquaculture.
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Linke, Dirk; Økstad, Ole Andreas; Haugen, Håvard Jostein & Winther-Larsen, Hanne Cecilie
(2021).
The exchange program MISC - Options for internships at the University of Oslo (with descriptions of current projects).
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Tandberg, Julia Isabel; Lagos, Leidy; Kashulin, Alexander; Sørum, Henning & Winther-Larsen, Hanne Cecilie
(2019).
Bacterial membrane vesicles as vaccines in aquaculture.
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Tandberg, Julia Isabel; Lagos, Leidy; Kashulin, Alexander; Langlete, Petter & Winther-Larsen, Hanne Cecilie
(2019).
Bacterial membrane vesicles as vaccines in aquaculture.
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Winther-Larsen, Hanne Cecilie
(2018).
Bacterial membrane vesicles: Implication in aquaculture
.
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Winther-Larsen, Hanne Cecilie
(2018).
Bacterial membrane vesicles: Implication in aquaculture
.
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Winther-Larsen, Hanne Cecilie
(2018).
Bacterial Membrane Vesicles in Therapeutics.
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Lampe, Elisabeth Opsjøn; Brudal, Espen; Tandberg, Julia Isabel; Lagos, Leidy; Sjöstedt, Anders & Griffiths, Gareth Wyn
[Vis alle 7 forfattere av denne artikkelen]
(2018).
Use of Zebrafish as Vaccine and Infection Model for Francisella.
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Vogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi & Hohmann-Marriott, Martin Frank
(2018).
A novel function of type IV pili in iron uptake in Synechococcus sp. PCC 7002.
Vis sammendrag
Iron is abundant in the Earth’s crust, but in marine environments, iron is often the limiting micronutrient for cyanobacterial growth. Marine cyanobacteria such as Synechococcus sp. PCC 7002 (hereafter Synechococcus) require iron for their photosynthetic machinery, especially Photosystem I, and have therefore developed efficient iron acquisition strategies. Recent research on type IV pili suggests that these so-called “nanowires” can increase metal bioavailability by facilitating reduction of extracellular electron acceptors such as iron oxides (1,2). These pili consist of tightly stacked pilA1 proteins and seem to have conductive properties (1).
We deleted the gene encoding pilA1 in Synechococcus and studied the growth of the deletion strain on different iron minerals with varying bioavailability. The ΔpilA1 strain shows reduced growth compared to wildtype Synechococcus when grown on iron oxides with low bioavailability.
We analyzed pili expression with SEM, TEM and mass spectrometry. Our results indicate a clear effect of pilA1 deletion on the expression of alternative pilins.
Finally, we assessed the state of the photosynthetic complexes by spectroscopic methods. Spectral analysis of the ΔpilA1 strain indicate changes of the photosynthetic complexes consistent with iron stress.
Our results indicate a novel function of type IV pili in iron acquisition in marine cyanobacteria. Heterologous pilA expression and deletion of genes involved in other parts of the external electron transport chain will further elucidate how intracellular electron transport is coupled to extracellular reduction.
(1) Gorby YA, Yanina S, McLean JS, et al (2006) Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms. Proc Natl Acad Sci U S A 103:11358–63. doi: 10.1073/pnas.0604517103
(2) Lamb JJ, Hill RE, Eaton-Rye JJ, Hohmann-Marriott MF (2014) Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803. PLoS One 9:e105761. doi: 10.1371/journal.pone.0105761
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Vogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi & Hohmann-Marriott, Martin Frank
(2018).
Conductive Type IV pili play an important role for iron acquisition in the marine cyanobacterium Synechococcus sp. PCC 7002 .
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Vogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi & Hohmann-Marriott, Martin Frank
(2018).
Function of type IV pili in iron acquisition in Synechococcus sp. PCC 7002.
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Vogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi & Hohmann-Marriott, Martin Frank
(2018).
Function of type IV pili in iron acquisition in Synechococcus sp. PCC 7002.
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Vogel, Anne Ilse Maria; Winther-Larsen, Hanne Cecilie; Roos, Norbert; Lale, Rahmi & Hohmann-Marriott, Martin Frank
(2018).
Function of type IV pili in iron acquisition in Synechococcus sp. PCC 7002.
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Brudal, Espen; Lampe, Elisabeth Opsjøn; Tandberg, Julia Isabel; Ulanova, Lilia; Winther-Larsen, Hanne Cecilie & Griffiths, Gareth Wyn
(2015).
Use of zebrafish as infection modell in aquaculture.
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Brudal, Espen; Julia Isabel, Tandberg; Sørum, Henning; Alexander, Kashulin & Winther-Larsen, Hanne C.
(2015).
Isolerte membranvesikler som vaksine mot francisellose og piscirickettsiose.
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Winther-Larsen, Hanne C.
(2014).
Bakterier og virus.
[Radio].
NRK.
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Brudal, Espen; Milton, Debra L; myrtennäs, Kerstin; Roos, Norbert; Griffiths, Gareth Wyn & Winther-Larsen, Hanne C.
(2014).
A degenerative Type IV pilus like system is functional and required for virulence in the fish pathogen Francisella noatunensis subsp. noatunensis.
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Elisabeth O., Lampe; Herrmann, Lydia; Brenz, Yannick Pirmin; Zingmark, Carl; Sjöstedt, Anders & Winther-Larsen, Hanne C.
[Vis alle 7 forfattere av denne artikkelen]
(2014).
Dissection of Francisella virulence in Dictyostelium; a genetically tractable host system.
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Karlsen, Christian; Ellingsen, Anetter Bauer; Wiik-Nielsen, Christer R.; Winther-Larsen, Hanne C.; Duncan J., Colquhoun & Sørum, Henning
(2014).
Distribusjon av virulensfaktorer og verts spesifisitet hos Moritella viscosa.
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Winther-Larsen, Hanne Cecilie
(2010).
Bio warfare research: help to find a remedy against francisellosis in fish?
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Winther-Larsen, Hanne Cecilie; Vanberg, Christin & Sørum, Henning
(2010).
Francisellosis - AFISH business.
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Winther-Larsen, Hanne Cecilie; Vanberg, Christin & Sørum, Henning
(2009).
Bioterrorisme-forskning bidrar til å løse Francisella problemet i torskeoppdrett.
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Salomonsson, Emelie; Winther-Larsen, Hanne Cecilie; Holz, Claudia; Maier, Berenike; Koomey, Michael & Forsberg, Åke
(2008).
Expressing Type IV pilin-like genes from Francisella tularensis in Neisseria gonorrhoeae.
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Salomonsson, Emelie; Koomey, Michael; Roos, Norbert; Forsberg, Åke & Winther-Larsen, Hanne C
(2007).
Exploring the role of virulence-associated pilin proteins from Francisella tularensis using heterologous expression in Neisseria.
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Salomonsson, Emelie; Koomey, Michael; Roos, Norbert; Forsberg, Åke & Winther-Larsen, Hanne C
(2007).
Knowledge about the bioterrorism class A agent Francisella tularensis by utilizing Neisseria gonorrhoeae.
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Winther-Larsen, Hanne C; Heiniger, Ryan W; Roos, Norbert; Egge-Jacobsen, Wolfgang; van Putten, JPM & Koomey, Michael
[Vis alle 7 forfattere av denne artikkelen]
(2006).
Dynamics of Type IV pili Biogenesis in Pseudomonas aeruginosa.
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Salomonsson, Emelie; Forsberg, Åke; Koomey, Michael & Winther-Larsen, Hanne C
(2006).
Heterologous expression of Francisella pilin genes in Neisseria gonorrhoeae.
Vis sammendrag
Analysis of the genome sequence of F. tularensis subsp. tularensis strain SCHU S4 has identified gene clusters encoding proteins postulated to be involved in protein secretion or in type IV pili (Tfp) expression. SCHU S4 encodes at least six putative pilin genes. We have recently verified that one of these genes, denoted pilA, is essential for full virulence in a mouse infection model. However, so far we have been unable to verify that F. tularensis expresses Tfp.or secreton related organelles To establish if any of the six pilin genes could form a pilus we expressed them, and in addition the pilA gene of subsp. novicida, in N. gonorrhoeae Western blot analysis verified that all pilins except one were expressed in this background. Expression of pilin filaments was analysed by immunofluorescence and electron microscopy and only PilA of F. novicida and SCHU S4 produced Tfp-like filaments. In support of PilA from F. novicida to form pilus-like multimers was that its expression also resulted in restored competence for DNA uptake in N. gonorrhoeae. However, at this point we cannot exclude that additional pilins are part of or can also form Tfp or a protein secretion related pseudopilus.
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Winther-Larsen, Hanne C; Heiniger, Ryan; Roos, Norbert; van Putten, JPM; Koomey, Michael & Wolfgang, Matthew
(2006).
Dynamics of Type IV pilus biogenesis in Pseudomonas aeruginosa.
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Løvold, Cecilia; Aas, Finn Erik; Winther-Larsen, Hanne C; Egge-Jacobsen, Wolfgang; Hegge, Finn Terje & Roos, Norbert
[Vis alle 8 forfattere av denne artikkelen]
(2005).
Mutant analysis of dca: a gene encoding a putative transferase that mediates phosphoethanolamine and phosphocholine modifications of the Type IV pili in Neisseria gonorrhoeae.
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Winther-Larsen, Hanne C; Løvold, Cecilia; Wolfgang, Matthew; van Putten, JPM; Roos, Norbert & Aas, Finn Erik
[Vis alle 10 forfattere av denne artikkelen]
(2005).
Pseudomonas aeruginosa PAK Type IV pili expression in Neisseria gonorrhoeae complements Tfp associated functions.
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Aas, Finn Erik; Løvold, Cecilia; Winther-Larsen, Hanne C; Jacobsen, Wolfgang Mathias; Roos, Norbert & Koomey, John Michael
(2004).
Characterization of dca, a gene involved in posttranslational modification of Neisseria gonorrhoeae Type IV Pili with phosphoethanolamine and phosphocholine.
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Løvold, Cecilia; Winther-Larsen, Hanne C & Aas, Finn Erik
(2004).
Transformation competence and human cell adherence by expression of the pseudomonas aeruginosa Type IV pilus pilin subunit in Neisseria gonorrhoeae.
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Løvold, Cecilia; Winther-Larsen, Hanne C; Aas, Finn Erik & Koomey, John Michael
(2004).
Complementation of both transformation competence and human cell adherence by hetrologous expression of the Pseudomonas aeruginosa Type IV pilin sunbunit in Neisseria gonorrhoeae.
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Winther-Larsen, Hanne C; Løvold, Cecilia & Koomey, John Michael
(2004).
Minor pilins involved in Type IV pilus biogenesis, retraction and achoring in Neisseria gonorrhoeae.
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Winther-Larsen, Hanne C; Løvold, Cecilia; Aas, Finn Erik; Roos, Norbert & Koomey, John Michael
(2004).
Complementation of both transformation competence and human cell adherence by hetrologous expression of the Pseudomonas aeruginosa Type IV pilin sunbunit in Neisseria gonorrhoea.
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Winther-Larsen, Hanne C; Løvold, Cecilia; Aas, Finn Erik; Roos, Norbert & Koomey, John Michael
(2004).
Complementation of both transformation competence and human cell adherence by hetrologous expression of the Pseudomonas aeruginosa Type IV pilin sunbunit in Neisseria gonorrhoea.
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Winther-Larsen, Hanne C; Løvold, Cecilia; Aas, Finn Erik; Roos, Norbert & Koomey, John Michael
(2004).
Complementation of both transformation competence and human cell adherence by hetrologous expression of the Pseudomonas aeruginosa Type IV pilin sunbunit in Neisseria gonorrhoea.
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Hegge, Finn Terje; Winther-Larsen, Hanne C & Koomey, M
(2003).
Evidence that the PilV protein of Neisseria gonorrhoeae interacts with the Tfp biogenesis machinery and influences post-translational modification of PilE, the pilin subunit.
Vis sammendrag
Neisseria gonorrhoeae depends on the expression of type IV pili (Tfp) to colonize epithelial cells of its human host. In order to express functional pili, more than 20 gene products must be active.
In addition to mediating cell adherence, the expression of functional pili also contributes to other phenotypes, including twitching motility, autoagglutination and competence for natural transformation.
PilV, a prepilin-like molecule, was identified by data mining of the gonococcal genome sequence due to its N-terminal sequence similarity to the major pilus subunit, PilE. Mutant analysis revealed that PilV is required for Tfp-mediated adherence but dispensable for the other Tfp associated phenotypes. PilV appears to function by promoting the proper display of the Tfp-associated adhesin PilC.
Here, we assessed the relevance of genes linked to pilV and identified an allele of pilV which results in a conditional disruption of Tfp biogenesis. Analysis of piliated revertants arising by extragenic suppressor mutations add a new level of complexity as to how it may function in promoting adherence.
In addition an altered pattern of migration of PilE on SDS-PAGE in various PilV backgrounds was discovered, indicating that PilV is directly or indirectly involved in post-translational modification of PilE.
