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Arous, Delmon; Pettersen, Erik O; Malinen, Eirik & Hellebust, Taran Paulsen
(2018).
Radiobiological plan evaluation in brachytherapy using two different cell survival models.
Radiotherapy and Oncology.
ISSN 0167-8140.
127,
p. 1233–1233.
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Pettersen, Erik O
(2017).
Noen sammenhenger mellom stråling og kreft: Og litt om Radon-problematikk på godt og vondt
.
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Rykkelid, Anne Marit; Siem, Sunniva; Görgen, Andreas; Ytre-Hauge, Kristian; Brondz, Efim & Sandvik, Joe Alexander
[Show all 9 contributors for this article]
(2017).
A high-resolution proton irradiator for in vitro studies of relative biological effectiveness.
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Arous, Delmon; Pettersen, Erik O; Malinen, Eirik & Hellebust, Taran Paulsen
(2017).
Radiotherapy cell survival curves for cervical cancer: Linear quadratic vs universal survival.
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Hole, Eli Olaug; Edin, Nina Frederike J; Malinen, Eirik; Pettersen, Erik O & Sagstuen, Einar
(2017).
Biophysics and medical physics at UiO: Research and education. An incomplete overview.
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Pettersen, Erik O
(2015).
EKKO; Abels tårn, Radonproblematikk.
[Radio].
NRK P2.
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Pettersen, Erik O
(2014).
The METOXIA project ends this summer: What was it all about? Who participated? Any results pointing forwards?
.
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Pettersen, Erik O
(2014).
Fysikk og kreft
.
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Pettersen, Erik O
(2014).
Stråling: Et naturprodukt med konsekvenser innen medisin og energi. Men hvordan virker stråling på oss?
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Pettersen, Erik O
(2013).
Summing up of METOXIA patenting and possibility for development of a hypoxia-specific drug.
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Pettersen, Erik O
(2013).
A short résumé of to what extent METOXIA reached its goals; planning round-up of the METOXIA project.
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Pettersen, Erik O
(2013).
Further development within the METOXIA-project period as guided by the CR-UK proposed programme. Coordinator's view.
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Pettersen, Erik O
(2013).
A brief summing up of the data produced so far by Cyprotex Discovery Ltd on compounds DTC-24a, Xanti-15a, DH348, NKP60, DH307, DH338 and Acetazolamide.
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Bousquet, Paula; Abshiru, Nebiyu; Johansen, Rune Forstrøm; Bjørås, Magnar; Sandvik, Joe Alexander & Pettersen, Erik O
[Show all 10 contributors for this article]
(2013).
A structural and functional study of anti-ganglioside antibodies.
International Journal of Molecular Medicine.
ISSN 1107-3756.
32,
p. S42–S42.
Show summary
NeuGc GM3 is considered an attractive target for cancer immunotherapy since it is a tumor-associated antigen present in several types of cancer such as breast carcinoma, melanoma and non-small cell lung cancer (NSCLC) while being effectively absent in healthy human tissues.
The monoclonal antibody (mAb) 14F7 is specific for NeuGc GM3 and does not cross-react with NeuAc GM3, a ganglioside found in healthy tissues, even though these two glycolipids have highly similar structures.
14F7 treatment has shown to induce complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC), but has in addition the ability to directly kill tumor cells without involving the complement system.
We employed a mass spectrometry-based approach and SPR analysis for the characterization of the binding interactions between 14F7 and the NeuGc GM3 ganglioside or the anti-idiotypic antibody generated from 14F7, 4G9, respectively.
In order to understand the mechanism by which the antibody kills the cells, we then performed stable isotope labeling with amino acids in cell culture (SILAC) combined with proteomics to identify and quantify proteins after antibody treatment. SILAC was also employed to investigate the protein expression in HeLa cells under hypoxic conditions. The combination of MS, SPR and SILAC analyses employed here have allowed us to gain a significantly improved understanding of the molecular interactions and functions of a highly promising antibody for cancer immunotherapies.
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Christoffersen, Stine; Sandvik, Joe Alexander; Pettersen, Erik O & Edin, Nina Frederike J
(2012).
Cell respiration, hypoxia and low dose rate radiation.
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Edin, Nina Frederike J; Pettersen, Erik O; Sandvik, Joe Alexander; Bergersen, Linda Hildegard; Chang, Cheng & Vollan, Hilde Synnøve
[Show all 9 contributors for this article]
(2012).
The role of TGFb3 and induced nitric oxide synthase activity in connection with induced resistance against low dose hyper-radiosensitivity.
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Pettersen, Erik O
(2012).
Further development within the METOXIA-project period with respect to sensor development and 3D in vitro models.
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Pettersen, Erik O
(2012).
Further development within the METOXIA-project period as guided by the CR-UK proposed programme.
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Pettersen, Erik O
(2012).
METOXIA: Tumour micro-environment for new specific cancer treatment.
Public Service Review: European Union.
ISSN 1472-3395.
23,
p. 222–223.
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Pettersen, Erik O & Ebbesen, Peter
(2012).
The scientific frame-work for this course and hypoxia and acidosis in human physiology and diseases.
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Pettersen, Erik O
(2012).
Hypoxia within METOXIA; possible follow-up?
Show summary
Jeg arrangerte en workshop under konferansen. Denne hadde tittelen: "Hypoxia and Cancer" og innheoldt 7 vitenskapelige foredrag.
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Pettersen, Erik O
(2011).
Some background and data for the METOXIA CAIX-inhibitor project.
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Pettersen, Erik O
(2011).
A short resume of to what extent METOXIA in its first two years reached its goals; an outline of where there are pressing problems for the project as a whole.
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Pettersen, Erik O
(2011).
Inven2 a/s and MetaSignal Therapeutics Inc and agreements.
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Pettersen, Erik O
(2011).
Patent situation and strategy to follow-up the PCT-application on our patented CA-IX inhibitors.
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Pettersen, Erik O
(2011).
Hva er vitenskapelige fakta om lavdosebestråling, radon og kreftutvikling.
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Pettersen, Erik O
(2011).
METOXIA-prosjektets tematikk og fagområde ved Fysisk Institutt.
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Pettersen, Erik O
(2011).
METOXIA - Hvordan bygge et storskala samarbeidkonsortium innen kreftforskning med et budsjett på over 15 millioner Euro.
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Edin, Nina Frederike J; Sandvik, Joe Alexander; Chang, Cheng; Vollan, Hilde Synnøve; Reger, Katharina & Görlach, Agnes
[Show all 8 contributors for this article]
(2011).
Molecular mechanisms for induction of sustained elimination of low dose hyper-radiosensitivity.
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Pettersen, Erik O
(2010).
Introductory remarks concerning patenting in METOXIA and the scientific basis of the 2 patents so far in question.
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Pettersen, Erik O
(2010).
Patent situation and strategy to demonstrate therapeutic and diagnostic proof-of-principle.
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Pettersen, Erik O
(2010).
METOXIA: The translation of new knowledge into cancer detection and treatment processes.
Public Service Review: European Union.
ISSN 1472-3395.
20,
p. 188–189.
Show summary
New methods to detect cancer micro-environmental parameters are developed alongside new therapeutic principles in the form of chemicals as possible new chemotherapeutic drugs and new methods to improve radiotherapy.
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Pettersen, Erik O
(2010).
To what extent has METOXIA in its first year reached its goals? Pressing problems for the project as a whole.
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Pettersen, Erik O
(2010).
Presentation of the METOXIA program.
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Kieninger, Jochen; Sandvik, Joe Alexander; Pettersen, Erik O; Jobst, Gerhard; Aravindalochanan, Kuppusamy & Urban, Gerald A
(2010).
Monitoring of peri-cellular oxygen levels in tumor cell cultures by amperometric oxygen sensor array.
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Aravindalochanan, K; Kieninger, Jochen; Sandvik, Joe Alexander; Pettersen, Erik O & Urban, Gerald A
(2009).
Optimising a nitric oxide sensing technique for hypoxic tumor cell cultures.
Show summary
A Nitric oxide (NO) electrochemical sensor for hypoxic, tumor cell culture applications is developed. Qualitative analysis of the electrode has been studied by cyclic voltammetry. NO amperometric measurement methods have been extensively analyzed. Square wave voltammetry method is found to be one of the optimal methods for the measurement of NO in cell cultures. A hypoxic tumor cell culture measurement (MCF-7 cell type) has also been conducted to observe the NO response to hypoxia. ©2009 IEEE.
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Pettersen, Erik O
(2009).
Plans for collaboration between clinicians and with pre-clinicians within METOXIA.
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Pettersen, Erik O
(2009).
Noen tanker om hypoksiens relevans for cellesyklus og strålefølsomhet.
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Pettersen, Erik O
(2009).
Noen poenger om hypoksiens relevans for cellesyklus, kreftutvikling og strålefølsomhet.
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Pettersen, Erik O
(2009).
The goals and structure of the METOXIA project and the project organisation.
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Pettersen, Erik O
(2009).
Hypoxic cell cycle and replication.
Show summary
An overview was given for the cell-cycle-regulation induced by hypoxia, as studied within EUROXY for the 5-year duration of the project.
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Pettersen, Erik O
(2009).
Implementing our plans. Our five year plan, and our stated goals for 2009 including the deliverables. The recommendation of the three-sub group meetings held in 2009. Decisions we must take during the present meeting. How did we get under way?
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Pettersen, Erik O
(2009).
Introduction and overview of purpose of meeting – project status.
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Pettersen, Erik O
(2009).
Introduction and overview of purpose of meeting – and project status.
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Pettersen, Erik O
(2008).
Krever mer av norske forskere.
[Newspaper].
Dagsavisen.
Show summary
det var en befaring ved Cellelabben, Fysisk Institutt, UiO av statsråd Tora Asland i forbindelse med at Erik Pettersen som koordinator hadde skaffet 100 millioner kroner til prosjektet METOXIA fra EUs 7. rammeprogram
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Pettersen, Erik O
(2008).
Dreper dødelige kreftceller; Norsk kreftfunn.
[Newspaper].
Verdens Gang.
Show summary
Ved å gi ultralav doserate bestråling fra en radioaktiv kilde inkorporert i krefcellenes protein har vi vist at hypoksiske kreftceller, som er resistente mot konvensjonell stråleterapi, kan gjøres strålefølsomme.
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Pettersen, Erik O
(2008).
Dreper de aller verste kreftcellene.
[Newspaper].
Østlendingen.
Show summary
Headingen var: En ny norsk metode kan vise seg å ta knekken på de aller tøffeste kreftcellene.
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Pettersen, Erik O
(2008).
Skal ta knekken på de tøffeste kreftcellene.
[Newspaper].
ABC Nyheter.
Show summary
Metoxia består av prosjekter som går ut på å finne forskjellige forsvarsmekanismer som cellene benytter seg av for å redusere sine skader på grunn av oksygenmangelen, og så behandle kreften ved å slå ut disse.
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Pettersen, Erik O
(2008).
Forskere ved Universitetet i Oslo vil drepe kreftceller ved å stråle dem fra innsiden av kroppen.
[Radio].
NRK P2, Programmet Verdt å vite.
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Pettersen, Erik O
(2008).
Svært lav stråling dreper resistente kreftceller.
[Newspaper].
Apollon, Forskningsmagasin fra Universitetet i Oslo, 4/2008.
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Edin, Nina Frederike J; Olsen, Dag Rune; Sandvik, Joe Alexander; Stokke, Trond & Pettersen, Erik O
(2008).
Mechanisms of the elimination of low dose hyper-radiosensitivity in T-47D cells by low dose-rate priming.
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Pettersen, Erik O
(2008).
Cancer Hypoxia in Retrospect.
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Pettersen, Erik O
(2008).
Cancer and Treatment.
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Pettersen, Erik O
(2008).
Gjennomslag i EUs rammeprogram - METOXIA, et samarbeidsprosjekt mellom universitet, sykehus og industri.
Show summary
Som prosjekt-koordinator for METOXIA var jeg invitert for å redegjøre for arbeidet med konsortiesammensetning og søknadsprosess for dette EU-prosjektet. Jeg forklarte litt om den historiske bakgrunnen for prosjektet, og viste hvorfor det er naturlig at en biofysiker koordinerer dette store medisinske prosjektet. Det ble spesielt diskutert hvor viktig en forskers posisjon i grunnforskningen er for at man skal få gjennomslag i EUs rammeprogrammer.
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Pettersen, Erik O
(2008).
EU-søknadsprosess fra start til kontrakt-samarbeid mellom ulike institusjoner.
Show summary
Jeg redegjorde for prosjektetablering og konsortiesamling for METOXIA, et "Collaborative Project" med økonomisk ramme på ca 100 mill NOK som nå er under forhandling med EU-kommisjonen og hvor jeg er prosjekt-koordinator.
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Pettersen, Erik O
(2008).
Fulfilling Promises.
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Pettersen, Erik O
(2008).
Strålefølsomhet for humane celler: Aspekter relatert til hypoksi, respirasjon og hypersensitivitet.
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Larsen, Benjamin Endre; Pettersen, Erik O; Karlsen, Jan; Sandvik, Joe Alexander & Melvik, Jan Egil
(2008).
Oxygen Metabolism in Cells Cultured in Alginate Matrices.
Show summary
Materials and methods
Cells from an established cell line of T-47D mammary carcinoma cells1 cultured in monolayer using RPMI supplemented with 10% foetal bovine serum was entrapped in alginate gels of approx. 1.0 mL. Gel formulations were prepared by quickly mixing the contents of two syringes containing sodium alginate solution (PRONOVA SLG-20 or SLM-20, FMC BioPolymer, Norway) and calcium alginate (PRONOVA
CaM, FMC BioPolymer) suspension with added cells, respectively, using a three way connector. The final alginate concentration was 2% w/w. The cell-containing alginate mixture was emptied into a small tube with 10 mm inner diameter.
A computer-connected optical oxygen sensor (NTH, flat-broken tip, Ø=140 μm, PreSens GmbH, Regensburg, Germany) was embedded in the gelling alginate mixture before it set, which occurred within minutes after initial mixing (fig 1). Growth medium was added in the tube above the gel. The oxygen
tension gradient in the gel and medium was also measured and logged for ~24 h using a Clarke-type micro-sensor (Ø = 8-12 μm) and profiling system with a micromanipulator, both computer-controlled (Unisense, Århus, Denmark)(fig. 2). Viability of the entrapped cells was registered by 3-D confocal microscopy on live/dead–stained (Calcein/Propidium iodide), vibratome sectioned gel slices. The parameters tested were alginate type and amount of oxygen initially present in the formulations.
Results and discussion
The oxygen level in the gels decreased rapidly after entrapment, depending on cell density. Data from three different gels (n = 3 in each set) with a starting cell concentration of 3,7·105 cells/mL are presented in fig. 1. The data indicate relatively constant oxygen consumption per cell with time until hypoxic conditions are reached. Oxygen concentration profiles taken in the vertical dimension of the system showed that oxygen concentration was homogeneous within the gel and increased linearly in the medium layer,
implying quasi-steady state diffusion (fig. 3). The tested T-47D cells were found to have an apparently constant oxygen consumption rate of 37±5 fmol/h/cell in mannuronate-rich gels, 60±10 fmol/h/cell in guluronate-rich gels and 100±20 fmol/h/cell in mannuronate-rich gels with suspended air-bubbles. This is significantly less than published data for T-47D cells in exponential growth.Confocal imaging showed that no proliferation had occurred at neither 3 nor 7 days. The plating efficiency was estimated to 56±8% for mannuronate-rich gels with or without suspended air bubbles, and 60±10% for guluronate-rich gels. Most studies on alginate gels report unimpaired diffusion of small molecules, compared to water, although macromolecular substances show lower diffusivity. As guluronate-rich gels have higher porosity than mannuronate-rich gels, the relative amount of guluronate in the gel, and the effect this has on macromolecular diffusion is a possible explanatory factor. The inclusion of air bubbles greatly increased the time needed to deplete oxygen stores in the system (fig.4). In this case, the oxygen tension as a function of time was non-linear and consistent with depletion of an additional oxygen compartment
in the system.
Conclusions
Alginate entrapped T-47D cells depleted the oxygen stores in the alginate gel structures as a function of time. Comparing guluronate- and mannuronate-rich gels demonstrated a significantly higher oxygen consumption rate by cells entrapped in the former gel type, although there was no significant difference between the plating efficiencies obtained. Adding an additional oxygen reservoir into the gel system increased the time-span during which oxygen was available in the matrix. No proliferation was registered in the examined system.
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Pettersen, Erik O
(2008).
Internasjonalisering sett med forskerblikk.
Show summary
Presentasjonen tok for seg utviklingen av internasjonale konsortier innen hypoksi-området av oncologien som har ledet frem til METOXIA-prosjektet. Utviklingen har gått over ca 15 år, ledet av Peter Ebbesen og Erik Pettersen og omhandler 5 forskjellige internasjonale konsortier, de to første i nordisk regi (NorFa og NICE) og de tre siste i EU-regi (Oxnorm, Euroxy og Metoxia). Betydningen av basalforskning for å danne denne typen konsortier ble diskutert med politikerne i Stortingets Kirke- Utdannings og Forskningskomité.
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Kieninger, Jochen; Dannenberg, Arne; Aravindalochanan, Kuppusamy; Jobst, Gerhard; Pettersen, Erik O & Urban, Gerald A
(2007).
Amperometric oxygen sensor array with novel chronoamperometric protocols for hypoxic tumor cell cultures.
Show summary
The paper is part of the METOXIA project
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Pettersen, Erik O
(2007).
Consortium answering the FP7 call: Health: Translating the hypoxic tumour microenvironment.
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Pettersen, Erik O
(2007).
Definitions of hypoxia in normal and pathological situations.
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Pettersen, Erik O
(2007).
Noen aspekter ved hypoksi, respirasjon og strålefølsomhet.
Show summary
Det ble gitt et bredt sammendrag av oksygenets rolle for cellevkst og strålefølsomhet. Spesielt ble det vektlagt hvordan lavdoserate bestråling påvirker cellenes respirasjonsrate. Det ble også gitt en oversikt over organisering av denne type forskning innen EUs rammeprogrammer.
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Edin, Nina Frederike J; Sandvik, Joe Alexander; Olsen, Dag Rune & Pettersen, Erik O
(2007).
LOW DOSE HYPER-RADIOSENSITIVITY IN T-47D CELLS.
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Edin, Nina Frederike J; Olsen, Dag Rune & Pettersen, Erik O
(2006).
Lav-dosis hypersensitivitet hos T-47D brystkræftceller.
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Edin, Nina Frederike J; Sandvik, Joe Alexander; Olsen, Dag Rune & Pettersen, Erik O
(2006).
Intracellular signalling through medium transfer and low dose hyper-radiosensitivity.
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Pettersen, Erik O
(2006).
7th framework program.
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Pettersen, Erik O
(2006).
An integrated project growing out of EUROXY.
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Pettersen, Erik O
(2006).
Extreme hypoxia at work.
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Pettersen, Erik O
(2006).
Development of methodology for characterization and definition of appropriate in vitro oxygenation for cancer research.
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Pettersen, Erik O
(2006).
WP2 at 24 months: Effects of low oxygen and mild radiation on cell cycling and survival.