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Hanson, Ingunn; Juvkam, Inga; Zlygosteva, Olga & Edin, Nina Frederike Jeppesen
(2023).
TGF-β3 increases the severity of radiation-induced oral mucositis and salivary gland fibrosis in a mouse model.
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Hanson, Ingunn; Juvkam, Inga; Zlygosteva, Olga & Edin, Nina Frederike Jeppesen
(2023).
TGF-β3 increases the severity of radiation-induced oral mucositis and salivary gland fibrosis in a mouse model.
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Edin, Nina Frederike Jeppesen
(2023).
Normal tissue responses in a murine model following fractionated irradiation of the head and neck with protons or X-rays
.
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Edin, Nina Frederike Jeppesen
(2023).
Preclinical proton therapy research at UiO and OUS.
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Edin, Nina Frederike Jeppesen
(2023).
The role of the immune system in radiation responses - are protons better than photons in inducing immunogenic signals?
.
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Rykkelid, Anne Marit; sinha, Priyanshu; Folefac, Charlemagne; Horsman, Michael; Sørensen, Brita Singers & Søland, Tine Merete
[Show all 9 contributors for this article]
(2023).
Synergistic effects of combining proton- or X-irradiation with anti-PDL1 immunotherapy in two murine oral cancers.
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Rykkelid, Anne Marit; sinha, Priyanshu; Folefac, Charlemagne; Horsman, Michael; Sørensen, Brita Singers & Søland, Tine Merete
[Show all 9 contributors for this article]
(2023).
Synergistic effects of combining proton- or X-irradiation with anti-PDL1 immunotherapy in two murine oral cancers.
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Edin, Nina Frederike Jeppesen
(2022).
PROCCA: Late effects after radiotherapy of the head and neck - from cells to persons.
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Hanson, Ingunn & Edin, Nina Frederike Jeppesen
(2022).
Shielding of the Femur Reduces the Severity of the Gastrointestinal Subsyndrome of ARS and Increases Survival in Mice.
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Nattudurai, Ravikumar; Arous, Delmon; Edin, Nina Frederike Jeppesen; Pandey, Anant & Malinen, Eirik
(2022).
Dosimetric characterization of BaSO4 phosphors activated by Eu for proton, gamma and X-ray beams.
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Hanson, Ingunn & Edin, Nina Frederike Jeppesen
(2022).
The role of TGF-β3 in modification of radiation response.
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Hanson, Ingunn & Edin, Nina Frederike Jeppesen
(2022).
Shielding of the femur reduces the severity of the gastrointestinal subsyndrome of ARS and increases survival in mice.
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Rykkelid, Anne Marit; Sørensen, Brita Singers; Horsman, Michael; Malinen, Eirik & Edin, Nina Frederike Jeppesen
(2022).
Effect of the Combination of Proton Radiotherapy and Immunotherapy on the MOC1/MOC2 tumor models in C57B16/Jrj mice.
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Nattudurai, Ravikumar; Arous, Delmon; Edin, Nina Frederike Jeppesen; Pandey, Anant & Malinen, Eirik
(2022).
Investigation of BaSO4:Eu nanophosphors for thermoluminescence dosimetry of X-ray and proton beams.
Radiotherapy and Oncology.
ISSN 0167-8140.
170.
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Edin, Nina Frederike Jeppesen
(2022).
Low dose radiation effects.
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Bousquet, Paula; Sandvik, Joe Alexander; Edin, Nina Frederike Jeppesen & Krengel, Ute
(2022).
Corrigendum to: Hypothesis: Hypoxia induces de novo synthesis of NeuGc gangliosides in humans through CMAH domain substitute (Biochemical and Biophysical Research Communications (2018) 495(1) (1562–1566), (S0006291X17323616), (10.1016/j.bbrc.2017.11.183)).
Biochemical and Biophysical Research Communications - BBRC.
ISSN 0006-291X.
587.
doi:
10.1016/j.bbrc.2021.11.074.
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Edin, Nina Frederike Jeppesen
(2022).
The present and future of FLASH-immunology.
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Hanson, Ingunn & Edin, Nina Frederike Jeppesen
(2021).
The role of TGF-B3 in modification of radiation response.
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Grigalavicius, Mantas; Mastrangelopoulou, Maria; Berg, K.; Arous, Delmon; Ménard, M. & Raabe-Henriksen, T.
[Show all 12 contributors for this article]
(2021).
Publisher Correction: Proton-dynamic therapy following photosensitiser activation by accelerated protons demonstrated through fluorescence and singlet oxygen production (Nature Communications, (2019), 10, 1, (3986), 10.1038/s41467-019-12042-7).
Nature Communications.
ISSN 2041-1723.
12(1).
doi:
10.1038/s41467-021-27175-x.
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Hanson, Ingunn; Pitman, Kathinka Elinor; Thiede, Bernd; Cai, Yiqing & Edin, Nina Frederike Jeppesen
(2021).
Proteomic Analysis of Low Dose-Rate Primed Cells and Extracellular Exosomes.
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Nattudurai, Ravikumar; Arous, Delmon; Edin, Nina Frederike Jeppesen & Malinen, Eirik
(2021).
Estimating linear energy transfer in proton beams using alanine/EPR dosimetry.
-
Edin, Nina Frederike Jeppesen
(2021).
Proton radiobiology studies at Oslo Cyclotron Laboratory.
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Nattudurai, Ravikumar; Arous, Delmon; Edin, Nina Frederike Jeppesen & Malinen, Eirik
(2021).
Alanine dosimeters for determining linear energy transfer in proton beams.
Radiotherapy and Oncology.
ISSN 0167-8140.
161,
p. 229–229.
-
Edin, Nina Frederike Jeppesen
(2020).
Increased immunogenic calreticulin signaling at the distal end of the proton Bragg peak
.
-
Zlygosteva, Olga; Malinen, Eirik & Edin, Nina Frederike J
(2020).
PROCCA: a new convergence environment.
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Hanson, Ingunn; Edin, Nina Frederike J; Thiede, Bernd & Altanerova, Ursula
(2020).
Communication of radioprotective efects by TGF-β3.
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Hanson, Ingunn; Altanerova, Ursula; Thiede, Bernd & Edin, Nina Frederike J
(2020).
Radioprotector TGF-β3 is secreted in exosomes and activated by LDR priming.
Show summary
The biological effects of ionizing radiation in the low-dose region is important for diagnostic imaging, healthy tissue in radiation therapy and environmental radiation. For a majority of tested cell lines, the low dose hyper-radiosensitive (HRS) response has been shown to be default. We have previously shown that cells primed with a low dose-rate (⁓0.3 Gy/h) ionizing radiation permanently loses the HRS response. We have also shown that the transforming growth factor (TGF)-β3 is responsible for this effect. Here, we investigated the secretion mechanism of TGF-β3 from LDR primed cells, and theorize regarding the way it is activated.
Exosomes were isolated from LDR primed cells, and were shown to remove HRS in reporter cells when co-cultured with these. Adding a TGF-β3 inhibitor to the medium restored the HRS response in reporter cells, confirming that TGF-β3 was the factor in the exosomes responsible for removing HRS. Mass spectrometry was performed on the content of exosomes from primed and control cells. Proteomic analysis confirmed the presence of exosome-specific proteins in exosomes from primed and control groups. TGF-β3 along with latency-associated protein (LAP), the protein that TGF-β3 is bound to in its inactive form, was present in similar amounts in both groups, indicating that TGF-β3 was secreted in its inactive form, regardless of LDR priming. Three proteins were significantly upregulated in the primed exosomes. These proteins will be investigated further in future experiments.
We conclude that TGF-β3 is secreted in its inactive form in exosomes from LDR primed and unprimed cells, and that the priming is responsible for its activation. We will further investigate three significantly upregulated proteins with regards to their role in the activation of TGF-β3.
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Edin, Nina Frederike J
(2019).
The role of TGF-beta3 in radioresistance.
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Edin, Nina Frederike J; Rykkelid, Anne Marit; Görgen, Andreas; Baker, Agnes; Siem, Sunniva & Ytre-Hauge, Kristian
[Show all 7 contributors for this article]
(2019).
Very high yield of double strand breaks found at the distal end of the proton Bragg peak.
Radiotherapy and Oncology.
ISSN 0167-8140.
133,
p. 594–594.
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Rykkelid, Anne Marit; Tormodsrud, Vera; Siem, Sunniva; Görgen, Andreas; Ytre-Hauge, Kristian & Malinen, Eirik
[Show all 7 contributors for this article]
(2019).
Very high RBE values found at the distal end of the proton Bragg peak.
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Rykkelid, Anne Marit; Siem, Sunniva; Ytre-Hauge, Kristian; Malinen, Eirik & Edin, Nina Frederike J
(2018).
Very high relative biological effectiveness found at the distal end of the proton Bragg peak.
Radiotherapy and Oncology.
ISSN 0167-8140.
127,
p. 1259–1259.
-
Rykkelid, Anne Marit; Siem, Sunniva; Görgen, Andreas; Ytre-Hauge, Kristian; Brondz, Efim & Sandvik, Joe Alexander
[Show all 9 contributors for this article]
(2017).
A high-resolution proton irradiator for in vitro studies of relative biological effectiveness.
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Hole, Eli Olaug; Edin, Nina Frederike J; Malinen, Eirik; Pettersen, Erik O & Sagstuen, Einar
(2017).
Biophysics and medical physics at UiO: Research and education. An incomplete overview.
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Dahle, Tordis Johnsen; Rykkelid, Anne Marit; Stokkevåg, Camilla Hanquist; Görgen, Andreas; Edin, Nina Frederike J & Malinen, Eirik
[Show all 7 contributors for this article]
(2017).
Monte Carlo simulations of a low-energy proton beam and estimation of LET distributions.
Radiotherapy and Oncology.
ISSN 0167-8140.
123,
p. 179–180.
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Frettem, Åshild Kvammen; Edin, Nina Frederike J; Galtung, Hilde & Søland, Tine Merete
(2016).
Tumour associated Aquaporin-3 and hypoxia in an oral squamous cell carcinoma cell line
.
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Edin, Nina Frederike J; altaner, cestmir; Altanerova, Veronica & Ebbesen, Peter
(2014).
TGF-3 IN PROTECTION AGAINST RADIATION DAMAGE.
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Edin, Nina Frederike J
(2014).
Induction of protection against radiation damage.
-
Christoffersen, Stine; Sandvik, Joe Alexander; Pettersen, Erik O & Edin, Nina Frederike J
(2012).
Cell respiration, hypoxia and low dose rate radiation.
-
Edin, Nina Frederike J
(2012).
Cycling hypoxia and inverse radiomodifying effects of oxygen at ultra-low radiation doses.
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Edin, Nina Frederike J; Pettersen, Erik O; Sandvik, Joe Alexander; Bergersen, Linda Hildegard; Chang, Cheng & Vollan, Hilde Synnøve
[Show all 9 contributors for this article]
(2012).
The role of TGFb3 and induced nitric oxide synthase activity in connection with induced resistance against low dose hyper-radiosensitivity.
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Edin, Nina Frederike J
(2012).
Hvor farlig er radioaktiv stråling?
-
Edin, Nina Frederike J & Ebbesen, Peter
(2012).
Effect of small doses whole-body γ-radiation given at low dose-rate to mice with normal micro-flora.
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Edin, Nina Frederike J; Sandvik, Joe Alexander; Chang, Cheng; Vollan, Hilde Synnøve; Reger, Katharina & Görlach, Agnes
[Show all 8 contributors for this article]
(2011).
Molecular mechanisms for induction of sustained elimination of low dose hyper-radiosensitivity.
-
Edin, Nina Frederike J
(2011).
The mechanism by which reoxygenation and low dose-rate radiation turn off hyper-radiosensitivity.
-
Edin, Nina Frederike J
(2011).
Low dose-rate gamma irradiation can protect against subsequent high dose-rate irradiation .
-
Edin, Nina Frederike J
(2011).
Molecular mechanisms for induction of sustained elimination of low dose hyper-radiosensitivity.
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Edin, Nina Frederike J; Olsen, Dag Rune; Sandvik, Joe Alexander; Stokke, Trond & Pettersen, Erik O
(2008).
Mechanisms of the elimination of low dose hyper-radiosensitivity in T-47D cells by low dose-rate priming.
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Edin, Nina Frederike J; Sandvik, Joe Alexander; Olsen, Dag Rune & Pettersen, Erik O
(2007).
LOW DOSE HYPER-RADIOSENSITIVITY IN T-47D CELLS.
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Edin, Nina Frederike J; Olsen, Dag Rune & Pettersen, Erik O
(2006).
Lav-dosis hypersensitivitet hos T-47D brystkræftceller.
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Edin, Nina Frederike J; Sandvik, Joe Alexander; Olsen, Dag Rune & Pettersen, Erik O
(2006).
Intracellular signalling through medium transfer and low dose hyper-radiosensitivity.
-
Edin, Nina Frederike J; Olsen, Dag Rune & Pettersen, Erik O
(2005).
Recovery of low-dose HRS following a small priming dose depends on priming dose rate.
-
Edin, Nina Frederike J
(2008).
Elimination of low dose hyper-radiosensitivity in T-47D cells. Then significance of dose-rate and oxygen level.
Det matematisk naturvitenskapelige fakultet.