In the TYPEVDSEC project, we will study the secretion and function of a patatin-like lipase from the pathogenic bacterium Pseudomonas aeruginosa. The results obtained in this project will provide deeper understanding of the biogenesis of PlpD and other type Vd secretion systems. As possible virulence factors of P. aeruginosa and other pathogens, type Vd secretion systems are a potential drug and vaccine target to combat hard-to-eradicate pathogens such as P. aeruginosa and emerging antibiotic resistance in these organisms.
Leo et al., Philos Trans R Soc Lond, Biol Sci. 367:1088-1101.
Type V secretion systems, also known as autotransporters, are the most widespread protein secretion systems in Gram-negative bacteria. Autotransporters consist of two functional units: the extracellular effector domain or passenger, and the transmembrane translocator, which is responsible for transporting the passenger to the outside of the cell. Currently, five subclasses of autotransporter are known. The aim of this project is to investigate the mechanism of the type Vd secretion pathway and compare it to the other, better characterised type V subclasses. To this end, we will centre on the type Vd-secreted lipase PlpD from Pseudomonas aeruginosa, an important pathogen especially in hospital settings. We will investigate how this lipase domain is secreted outside the cell and the role of the lipase in virulence using both animal and plant model systems.
Funded by an RCN Young Researcher Grant (#249793).