NMR and Metabolomics

Investigation of a variety of disease states by NMR in connection with statistical analysis.

About the project

We are currently in the process of starting up several NMR metabolomic studies.

One definition of the concept of metabolomics:

Metabolomics is the "systematic study of the unique chemical fingerprint the specific cellular processes leave behind" – specifically, the study of their small-molecule metabolite profiles. The metabolome represents the collection of all metabolites in a biological organism, which are the end products of its gene expression. Thus, while mRNA gene expression data and proteomic analyses do not tell the whole story of what might be happening in a cell, metabolic profiling can give an instantaneous snapshot of the physiology of that cell. One of the challenges of systems biology and functional genomics is to integrate proteomic, transcriptomic and metabolomic information to give a more complete picture of living organisms. From wikipedia

 

Objectives

A variety of disease states are going to be investigated by NMR in connection with statistical analysis. One dimensional water suppressed 1H NMR spectra for selected subsets of TOCSY spectra are to be acquired in automation at 600 MHz. Approximately 1000 samples are to be run in connection with investigations about diabetes in the pregnancy. We also plan to investigate samples from the Oslo Health Study (HUBRO) conducted in the City of Oslo from May 2000 to September 2001. Samples from HUBRO will be analyzed by statistical analyses (AMIX) to identify persons with thyroid diseases. Cushing (increased cortisol production), acromegaly (increased level of growth hormone) and heart diseases are diseases where metabolic profiling of urine can result in new diagnostic possibilities and give insight in patophysiologic mechanisms. Mutation in the ABC transporter ABCC6 is giving rise to the illness preudoxanthoma elasticum, but the molecular identity of the substrate being transported by ABCC6 is currently unknown, the identity of the substrate will be investigated by metabolomics.

Cooperation

Researchers at Department of Medicinal Biochemistry and Clinical Pharmacology, Oslo University Hospital (Ullevål). Prof. Jens P. Berg and Chief Physician Armin Pielher, and Prof. Frode Rise at Department of Chemistry, UiO, are currently in the process of starting up several NMR metabolomic studies.

Tools

NMR instruments from University of Oslo NMR Center are used in these studies.

Published Jan. 11, 2011 3:18 PM - Last modified Feb. 28, 2014 8:35 AM