Using high throughput sequencing to look for unknown pathogens
Friday seminar by Torstein Tengs
The recent decrease in costs associated with DNA sequencing has allowed for the development of new methods for pathogen discovery using culture-independent methods. We have used high throughput sequencing and bioinformatics to identify novel pathogens, primarily in farmed Atlantic salmon. Two new viruses have been described; piscine reovirus (PRV) in heart and skeletal muscle inflammation (HSMI) and a novel totivirus (piscine myocarditis virus; PMCV) that appears to be associated with cardiomyopathy syndrome (CMS). The approach shows great merit and should be widely applicable to other systems. We have also attempted to identify the causative agent responsible for the massive death of Norwegian muskoxen in 2012 using the same strategy.
Torstein Tengs, PhD
Norwegian Veterinary Institute, Section for virology
Ullevaalsveien 68, 0454 Oslo