About the project
We propose a project characterized by exclusive use of new ultra-high-throughput sequencing technology combined with state-of-the art bioinformatics algorithms designed for maximal exploitation of this technology. The project will be carried out in close collaboration with 454 Life Sciences, the inventor and manufacturer of the 454 sequencing technology. Sequencing will be based on a BAC library of more than 92.000 clones (average insert site 125 kb). We anticipate a final coverage of 6x of the cod genome. The annotation work will be lead by the Norwegian team but will involve 454 Life Sciences, the European Bioinformatics Institute and other highly competent actors with documented previous annotation experience. This sequencing project will provide a smooth competence transfer to Norwegian researchers concerning 454 sequencing, assembly protocols and annotation methodology The proposed project will be one of the first of its kind where the new technology is used to de novo sequence a complete and large vertebrate genome at a low cost.
Financing
This project is funded by the Research Council of Norway.
Period
Start: 1.11.2008. End: 31.12.2011.