Moflo Astrios Eq

MoFlo Astrios is a high speed cell sorter that can be outfitted with different nozzle sizes (70 and 100 μm). With 4 lasers, 14 color detectors, and ability to detect particles as small as 200 nm using the forward scatter detector, Astrios is the most performant sorter in our lab. Maximum sorting speed with the 70 μm nozzle is 25,000 events/second (approx. 90 million events/hour); with 100 μm nozzle the maximum sorting speed is 10,000 events/second (or 36 million events/hour).

Sorter setup

The Moflo Astrios setup can be altered to a degree depending on the cell type and fluorochromes used therefore it is essential we know the details of these in advance. Sorter laser wavelengths and measurable fluorochromes are shown in figure 1 and table 1.

Figure 1. The MoFlo Astrios is equipped with 4 laser/4 pinhole configurable system; 355 nm (UV), 488 nm (blue), 561 nm (green), 640 nm (red).
Table 1: Overview of available lasers and detectors

Specifications

  • Nozzle sizes of 70 and 100 μm
  • Sample Tubes: 5ml or 15ml
  • Collection Tubes: 5, 15 and 50ml
  • Plates: 6, 12, 24,96 and 384 well
  • Slides
  • Collect up to 6 populations
  • Sterile Sorting
  • Nozzles/Pressure: 70um / 60psi and 100um / 30psi
  • Speeds: 70um – 20,000 evts/sec and 100um – 6,000 evts/sec
  • Cooling system
  • Sheath: Beckman Coulter Isoflow