Denis Zosen

Yadav, A., Amber, M., Zosen, D., Labba, N.A., Huiberts, E.H.W., Erdem, J.S., Haugen, F., Berntsen, H.F., Zienolddiny, S., Paulsen, R.E. and Ropstad, E., 2020. A human relevant mixture of persistent organic pollutants (POPs) and perfluorooctane sulfonic acid (PFOS) enhance nerve growth factor (NGF)-induced neurite outgrowth in PC12 cells. Toxicology Letters. https://doi.org/10.1016/j.toxlet.2020.12.007

Zosen, D.V., Dorofeeva, N.A., Chernigovskaya, E.V., Bachteeva, V.T. and Glazova, M.V., 2018. ERK1/2 inhibition increases dopamine release from differentiated PC12 cells. Neuroscience letters, 684, pp.6-12. https://doi.org/10.1016/j.neulet.2018.06.056

Saparova, V. B., Zosen, D. V., Nasluzova, E. V., Berezovskaya, A. S., Bakhteeva, V. T., Gorbacheva, E. L., Chernigovskaya, E. V., & Glazova, M. V. (2019). Analysis of Neural Stem Cells from Krushinskii–Molodkina Rats, Which Have a Genetic Predisposition to Audiogenic Seizures. Neuroscience and Behavioral Physiology, 49(6), 765-772. https://doi.org/10.1007/s11055-019-00799-9

Dorofeeva, N.A., Nikitina, L.S., Zosen, D.V., Glazova, M.V. and Chernigovskaya, E.V., 2017. Functional state of the nigrostriatal system of Krushinsky–Molodkina rats during audiogenic seizure expression. Russian Journal of Genetics: Applied Research, 7(3), pp.217-225. https://doi.org/10.1134/S2079059717030029

Zosen, D. V., and M. V. Glazova. "The Role of the Interaction of p53 and the MAPK Cascade in Controlling Neuronal Differentiation in the PC12 Cell Line." Neuroscience and Behavioral Physiology 46.5 (2016): 559-565. https://doi.org/10.1007/s11055-016-0277-y

• Yadav, Ajay; Amber, Mazia; Zosen, Denis; Labba, Nils Anders; Huiberts, Eva Henriette Willemijn; Samulin-Erdem, Johanna Maria; Haugen, Fred; Berntsen, Hanne Friis; Zienolddiny, Shanbeh; Paulsen, Ragnhild Elisabeth; Ropstad, Erik; Connolly, Lisa & Verhaegen, Steven (2020). A human relevant mixture of persistent organic pollutants (POPs) and perfluorooctane sulfonic acid (PFOS) enhance nerve growth factor (NGF)-induced neurite outgrowth in PC12 cells. Toxicology Letters.  ISSN 0378-4274.  338, s 85- 96 . doi: 10.1016/j.toxlet.2020.12.007 Fulltekst i vitenarkiv. Vis sammendrag

  Disruption of neurite outgrowth is a marker for neurotoxicity. Persistent organic pollutants (POPs) are potential developmental neurotoxicants. We investigated their effect on neurite outgrowth in PC12 rat pheochromocytoma cells, in absence or presence of nerve growth factor (NGF), an inducer of neuronal differentiation. Cells were exposed for 72 h to a defined mixture of POPs with chemical composition and concentrations based on blood levels in the Scandinavian population. We also evaluated perfluorooctane sulfonic acid (PFOS) alone, the most abundant compound in the POP mixture. Only higher concentrations of POP mixture reduced tetrazolium salt (MTT) conversion. High-content analysis showed a decrease in cell number, but no changes for nuclear and mitochondrial cellular health parameters. Robust glutathione levels were observed in NGF-differentiated cells. Live imaging, using the IncuCyte ZOOM platform indicated ongoing cell proliferation over time, but slower in presence of NGF. The pollutants did not inhibit neuritogenesis, but rather increased NGF-induced neurite length. PFOS induced neurite outgrowth to about 50 % of the level seen with the POP mixture. Neither the POP mixture nor PFOS affected neurite length in the absence of NGF. Our observations indicate that realistic complex mixtures of environmental pollutants can affect neuronal connectivity via NGF-induced neurite outgrowth.