PeptoMicelles, PeptoPlexes and NanoPeptoGels: Functional Drug and Gene Delivery Systems based on Endogenous Amino Acids
Seminar by Dr. Matthias Barz, Institute of Organic Chemistry, Johannes Gutenberg University Mainz, Germany
Nanomedicines posses the enormous potential to adjust pharmacokinetics of incorporated drugs, combine active agents and deliver them simultaneously or sequentially to finally improve therapeutic or diagnostic tools. One hand the particle properties, e.g. size, shape and functionality, need to be adjusted to the medical need. On the other, the material used for nanoparticle needs be biocompatible, non-immunogenic and excretable or with respect to long-term application better metabolizable within the body after performing the desired action. In addition, the nanoparticle platform itself should be as simple and versatile as possible to enable clinical translation.
With respect to those needs we have developed different classes of nnanomedicines, e.g. PeptoMicelles, PeptoNanoGels and PeptoPlexes, based on well-defined functional polymers named polypept(o)ides. These block copolymers are completely based on endogenous amino acids.
While polysarcosine is used as a hydrophilic block ensuring “stealth-like” properties (hydrophilicity, reduced protein interaction and non-immunogenicity), proteinogenic amino acids, e.g. lysine, glutamic acid and cysteine, provide the desired chemical functionalities. To prevent premature disintegration in vivo all nanoDDS are stabilized by disulfide bonds, which are stable in blood circulation but cleaved intracellular.
Based on this technology we are able to synthesize different nanomedicines and control their size, shape and functionality. First therapeutic applications of PeptoNanoGels (siRNA delivery), PeptoPlexes (pDNA delivery) and PeptoMicelles (drug delivery and imaging) are presented.