BMB Section seminar: "How the histone variants macroH2A function in metabolism and genome architecture"
Marcus Buschbeck, Principal Investigator and Group leader at IMPPC and Josep Carreras Institute (IJC), Campus Can Ruti, Badalona, Spain
Marcus Buschbeck (Photo: http://www.carrerasresearch.org/)
The macroH2A histone variants contribute to the establishment and maintenance of differentiated states. As a consequence macroH2As play an important role in development, somatic cell reprogramming and cancer. In contrast to all other histones macroH2A contain a long flexible unstructured C-terminal linker that places a globular metabolite binding domain outside of the nucleosome. How macroH2A histone variants function on the molecular level is still unclear.
I will present novel data demonstrating a role of macroH2A proteins in two key processes: the organization of genome architecture and the regulation of metabolism.
First, I will explain how macroH2A regulates the architecture of heterochromatin in the three-dimensional nuclear space by mediating the interaction with proteins of the inner nuclear envelope. Second, I will describe how a switch in splicing of the primary macroH2A1-encoding transcript mediates a shift in the consumption of NAD+ from the nucleus to the mitochondria.