EVOGENE seminar - Anders Krabberød and Marit Bjorbækmo
This seminar will consist of two 20 minutes talks entitled:
"Expanding our knowledge about host-symbiont interactions using PATHOS-DB"
"Single cell -omics: From a single cell to a sequence hell."
"Expanding our knowledge about host-symbiont interactions using PATHOS-DB", Marit Bjorbækmo, EVOGENE
In order to identify and gain knowledge about symbionts we have developed a database. The massive numbers of sequences generated through high-throughput sequencing projects over the recent years have revealed that there is a mindboggling diversity of protists in all environments investigated. To expand our knowledge beyond the mere number of ’species’ occurring in an environment and to interpret ecological functions is a demanding task.
Our database can be used to obtain information about what hosts the symbionts are associated with – or the other way around, to obtain information about different symbionts associated with a specific host. In this presentation I will show how the database can be applied to examine what is known about protistan symbionts (and their host-associations) in general. I will also demonstrate how the database can be used to detect protistan parasites in environmental samples, and how it can be used to reveal unknown putative parasites and infer their host-association.
"Single cell -omics: From a single cell to a sequence hell", Anders Krabberød, EVOGENE
In the last few years methodological advancements has made it possible to extract, amplify and sequence both genomes and transcriptomes from single cells. This opens up for a lot of different exiting possibilities both for those working with clinical material and cell cultures, as well as for biologists working with unculturable organisms (such as myself). For instance it will be possible to look at genetic variability and expression of genes between individual cells in a population, whether it is from a tissue sample, cell culture or a natural sample. I will give an overview of some of the different methods that has been developed and the advantages of single cell resolution and I will present some of my own experiences, successes and setbacks after several years of working with single cells.