EVOGENE Seminar - Insights into the autotransport mechanism of Yersinia adhesin A (YadA)
Nandini Chauhan, EVOGENE
Abstract: Gram negative bacteria have an inner and outer membrane surrounding them. The outer membrane proteins have to cross these barriers in order to either insert into the outer membrane or for secretion into the extracellular space. There are about nine secretion systems that help these proteins to traverse accross the membranes by different mechanisms. YadA is an outer membrane protein present in Yersinia enterocolitica and Yersinia pseudotuberculosis. It functions as an adhesin and plays an important role in the pathogenesis process. It has a C-terminal β-barrel domain which anchors the protein to the outer membrane followed by a coiled coil stalk that passes through the pore of the barrel and a globular head domain, together known as passenger domain. The mechanism by which the N-terminal passenger domain is translocated through the outer membrane is still under debate. A flexible region of residues has been indentified near the C terminus of the passenger domain which have been speculated to be involved in the transport process . My project is to mutate the residues of this region with an idea to capture translocation intermediates and also to study the role of this region in the transport process.