Jens Hjerling Leffler: Cell types in the mouse cortex revealed by single-cell RNA-sequencing
Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-sequencing
The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviors. Normal brain function relies on a diverse set of differentiated cell types, including neurons, glia, and vasculature.Here,we have used large-scale single-cell RNA sequencing (RNA-seq) to classify cells in themouse somatosensory cortex and hippocampal CA1 region.We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex.We identified numerous marker genes, which allowed alignment with known cell types, morphology, and location.We found a layer I interneuron expressing Pax6 and a distinct post mitotic oligodendrocyte subclass marked by Itpr2. Across the diversity of cortical cell types, transcription factors formed a complex, layered regulatory code, suggesting amechanism for the maintenance of adult cell type identity.
Bio: Jens Hjerling Leffler
Jens is an associate professor at the Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Sweden and a member of the Young academy of Sweden. After his undergraduate studies at Lund University and Max Delbrueck Center in Berlin he did his PhD at Karolinska Institutet on the development of the sensory nervous system. After a shorter postdoc at University College London with Martin Koltzenburg he joined the lab of Gord Fishell at NYU School of Medicine before returning to Sweden in 2011 to establish his own laboratory. He was an EMBO and a SSMF fellow and is currently an ENI-NET fellow. His current work is supported by a senior grant from Swedish Research Council as well as the Wellcome Trust, Swedish Brain Foundation and StratNeuro. His group’s research is focused on how functional neuronal identity is acquired and regulated during brain maturation and during different brain states from a molecular and network point of view using telencephalic interneurons as a model system. The laboratory applies methods such as modern mouse genetics, viral techniques, single-cell sequencing, electrophysiology and behavioral analysis to analyze the role of distinct cell classes in normal behavior as well as a putative role in disease.