Steven Ray Haakon Wilson

(Recent, selected publications)

"18.8 Tesla NMR spectroscopy: an effective platform for studying glioblastoma cells´ response to a cancer drug". Ingvild Comfort Hvinden, Henriette Engen Berg, Daniel Sachse, Erlend Skaga, Elsa Lundanes, Cecilie J. Sandberg, Einar O. Vik-Mo, Frode Rise, Steven Ray Wilson J. Proteome. Res. 2019, DOI: 10.1021/acs.jproteome.8b00801.

"Online peptide epitope fishing – nanoLC-MS of protein biomarkers". Maren C. S. Levernæs, Ole Kristian Brandtzaeg, Léon Reubsaet, Elsa Lundanes, Trine G. Halvorsen, Steven Ray Wilson, Anal. Chem. 2018, 90 (23), pp 13860–13866

"A multi-channeled open tubular enzyme reactor on-line coupled with mass spectrometry for detecting ricin". Ole Kristian Brandtzaeg, Bent-Tore Roen, Siri Enger, Elsa Lundanes, Steven Ray Wilson. Anal. Chem. 2017 89 (17), 8667-8673

"Mass spectrometric detection of 27-hydroxycholesterol in breast cancer exosomes". Roberg-Larsen H, Lund K, Seterdal KE, Solheim S, Vehus T, Solberg N, Krauss S, Lundanes E, Wilson SR. J. Steroid. Biochem. Mol. Biol. 2017 169, 22-28

"Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum". Brandtzaeg OK, Johnsen E, Roberg-Larsen H, Seip KF, MacLean EL, Gesquiere LR, Leknes S, Lundanes E, Wilson SR. Sci Rep 2016. 6, Article number: 31693.

• Bergh, Marianne Skov-Skov; Bogen, Inger Lise; Nerem, Elisabeth; Wohlfarth, Ariane; Wilson, Steven Ray Haakon & Øiestad, Åse Marit Leere (2021). Discovering the major metabolites of the three novel fentanyl analogues 3-methylcrotonylfentanyl, furanylbenzylfentanyl, and 4-fluorocyclopropylbenzylfentanyl for forensic case work. Forensic Toxicology.  ISSN 1860-8965.  39, s 167- 178 . doi: 10.1007/s11419-020-00560-9
• Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon & Lundanes, Elsa (2021). Recent advances in on-line upfront devices for sensitive bioanalytical nano LC methods. TrAC. Trends in analytical chemistry.  ISSN 0165-9936.  136, s 1- 10 . doi: 10.1016/j.trac.2021.116190
• Skottvoll, Frøydis Sved; Hansen, Frederik; Harrison, Sean; Boger, Ida Caroline Sneis; Mrša, Ago; Restan, Magnus Saed; Stein, Matthias; Lundanes, Elsa; Pedersen-Bjergaard, Stig; Aizenshtadt, Aleksandra; Krauss, Stefan; Sullivan, Gareth; Bogen, Inger Lise & Wilson, Steven Ray Haakon (2021). Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies. Analytical Chemistry.  ISSN 0003-2700. . doi: 10.1021/acs.analchem.0c05082
• Zawadzka, Malgorzata Elzbieta; Racz, Beatrix; Ambrosini, Dario; Görbitz, Carl Henrik; Morth, Jens Preben; Wilkins, Alistair L.; Østeby, Anja; Elgstøen, Katja B. Prestø; Lundanes, Elsa; Rise, Frode; Ringvold, Amund & Wilson, Steven Ray Haakon (2021). Searching for a UV-filter in the eyes of high-flying birds. Scientific Reports.  ISSN 2045-2322.  11(1) . doi: 10.1038/s41598-020-79533-2
• Abadpour, Shadab; Aizenshtadt, Aleksandra; Olsen, Petter Angell; Shoji, Kayoko; Wilson, Steven Ray Haakon; Krauss, Stefan & Scholz, Hanne Bjørnson (2020). Pancreas-on-a-Chip Technology for Transplantation Applications. Current Diabetes Reports.  ISSN 1534-4827.  20 . doi: 10.1007/s11892-020-01357-1 Full text in Research Archive.
• Harrison, Sean; Siller, Richard; Tanaka, Yoshiaki; Xiang, Yangfei; Patterson, Benjamin; Kempf, Henning; Melum, Espen; Åsrud, Kathrine Sivertsen; Dugarte, Maria Eugenia Chollet; Andersen, Elisabeth; Sandset, Per Morten; Baumgarten, Saphira Felicitas; Bonanini, Flavio; Kurek, Dorota; Mathapati, Santosh Sadashiv; Almaas, Runar; Sharma, Kulbhushan; Wilson, Steven Ray Haakon; Skottvoll, Frøydis Sved; Boger, Ida Caroline Sneis; Bogen, Inger Lise; Nyman, Tuula Anneli; Wu, Jun J.; Bezrouk, Ales; Cizkova, Dana; Mokrý, Jaroslav; Zweigerdt, Robert; Park, In-Hyun & Sullivan, Gareth (2020). Scalable production of tissue-like vascularised liver organoids from human PSCs.. BioRxiv.  ISSN 0362-4331. . doi: 10.1101/2020.12.02.406835
• Kogler, Stian; Harrison, Sean; Aizenshtadt, Aleksandra; Skottvoll, Frøydis Sved; Abadpour, Shadab; Krauss, Stefan; Scholz, Hanne; Sullivan, Gareth; Lundanes, Elsa & Wilson, Steven Ray Haakon (2020). “Organoid-in-a-column” coupled on-line with liquid chromatography-mass spectrometry. BioRxiv.  ISSN 0362-4331. . doi: https://doi.org/10.1101/2020.09.08.282756
• Sue Carter, Carter; Kenkel, William M.; MacLean, Evan L.; Wilson, Steven Ray Haakon; Perkeybile, Allison M.; Yee, Jason R.; Ferris, Craig F.; Nazarloo, Hossein P.; Porges, Stephen W.; Davis, John M.; Connelly, Jessica J. & Kingsbury, Marcy A. (2020). Is oxytocin “nature’s medicine”?. Pharmacological Reviews.  ISSN 0031-6997.  72(4), s 829- 861 . doi: 10.1124/pr.120.019398 Full text in Research Archive.
• Tsjokajev, Ahmad; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon; Anne Berit Dyve, Lingelem; Skotland, Tore; Sandvig, Kirsten & Lundanes, Elsa (2020). Mass spectrometry-based measurements of cyclic adenosine monophosphate in cells, simplified using reversed phase liquid chromatography with a polar characterized stationary phase. Journal of chromatography. B.  ISSN 1570-0232.  1160, s 1- 6 . doi: 10.1016/j.jchromb.2020.122384 Full text in Research Archive.
• Bergh, Marianne Skov-Skov; Bogen, Inger Lise; Wohlfarth, Ariane; Wilson, Steven Ray Haakon & Øiestad, Åse Marit Leere (2019). Distinguishing Between Cyclopropylfentanyl and Crotonylfentanyl by Methods Commonly Available in the Forensic Laboratory. Therapeutic Drug Monitoring.  ISSN 0163-4356.  41(4), s 519- 527 . doi: 10.1097/FTD.0000000000000617 Full text in Research Archive.
• Hvinden, Ingvild Comfort; Berg, Henriette Engen; Sachse, Daniel; Skaga, Erlend; Skottvoll, Frøydis Sved; Lundanes, Elsa; Sandberg, Cecilie; Vik-Mo, Einar O.; Rise, Frode & Wilson, Steven Ray Haakon (2019). Nuclear Magnetic Resonance Spectroscopy to Identify Metabolite Biomarkers of Nonresponsiveness to Targeted Therapy in Glioblastoma Tumor Stem Cells. Journal of Proteome Research.  ISSN 1535-3893.  18(5), s 2012- 2020 . doi: 10.1021/acs.jproteome.8b00801
• Lin, Ann; Skottvoll, Frøydis Sved; Rayner, Simon; Pedersen-Bjergaard, Stig; Sullivan, Gareth; Krauss, Stefan; Wilson, Steven Ray Haakon & Harrison, Sean (2019). 3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry. Electrophoresis.  ISSN 0173-0835.  41, s 56- 64 . doi: 10.1002/elps.201900170 Full text in Research Archive.
• MacLean, Evan L.; Wilson, Steven Ray Haakon; Martin, W. Lance; Davis, John; Nazarloo, Hossein P. & Carter, C. Sue (2019). Challenges for measuring oxytocin: The blind men and the elephant?. Psychoneuroendocrinology.  ISSN 0306-4530.  107, s 225- 231 . doi: 10.1016/j.psyneuen.2019.05.018 Full text in Research Archive.
• Olsen, Christine; Skottvoll, Frøydis Sved; Brandtzaeg, Ole Kristian; Schnaars, Christian; Rongved, Pål; Lundanes, Elsa & Wilson, Steven Ray Haakon (2019). Investigating Monoliths (Vinyl Azlactone-co-Ethylene Dimethacrylate) as a Support for Enzymes and Drugs, for Proteomics and Drug-Target Studies. Frontiers in Chemistry.  ISSN 2296-2646.  7 . doi: 10.3389/fchem.2019.00835
• Solheim, Stian; Hutchinson, Samantha A; Lundanes, Elsa; Wilson, Steven Ray Haakon; Thorne, James L & Røberg-Larsen, Hanne (2019). Fast liquid chromatography-mass spectrometry reveals side chain oxysterol heterogeneity in breast cancer tumour samples. Journal of Steroid Biochemistry and Molecular Biology.  ISSN 0960-0760.  192 . doi: 10.1016/j.jsbmb.2019.02.004 Full text in Research Archive.
• Wilson, Steven Ray Haakon; Olsen, Christine & Lundanes, Elsa (2019). Nano liquid chromatography columns. The Analyst.  ISSN 0003-2654.  144(24), s 7090- 7104 . doi: 10.1039/c9an01473j Full text in Research Archive.
• Bergh, Marianne Skov-Skov; Bogen, Inger Lise; Wilson, Steven Ray Haakon & Øiestad, Åse Marit Leere (2018). Addressing the Fentanyl Analogue Epidemic by Multiplex UHPLC-MS/MS Analysis of Whole Blood. Therapeutic Drug Monitoring.  ISSN 0163-4356.  40(6), s 738- 748 . doi: 10.1097/FTD.0000000000000564
• Dias, Irundika H.K.; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2018). Chromatography of oxysterols. Biochimie.  ISSN 0300-9084. . doi: 10.1016/j.biochi.2018.05.004
• Levernæs, Maren Christin Stillesby; Brandtzaeg, Ole Kristian; Amundsen, Sunniva Furre; Reubsaet, Leon; Lundanes, Elsa; Halvorsen, Trine Grønhaug & Wilson, Steven Ray Haakon (2018). Selective Fishing for Peptides with Antibody Immobilized Acrylate Monoliths, Coupled Online with NanoLC-MS. Analytical Chemistry.  ISSN 0003-2700.  90, s 13860 . doi: 10.1021/acs.analchem.8b00935
• Skottvoll, Frøydis Sved; Berg, Henriette Engen; Bjørseth, Kamilla; Lund, Kaja; Roos, Norbert; Bekhradnia, Sara; Thiede, Bernd; Sandberg, Cecilie; Vik-Mo, Einar O.; Røberg-Larsen, Hanne; Nyström, Bo; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). Ultracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations. Future science OA.  ISSN 2056-5623.  5(1) . doi: 10.4155/fsoa-2018-0088 Full text in Research Archive.
• Berg, Henriette Sjånes; Sæterdal, Kristina Erikstad; Smetop, Tone; Rozenvalds, Rūdolfs; Brandtzaeg, Ole Kristian; Vehus, Tore; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Self-packed core shell nano liquid chromatography columns and silica-based monolithic trap columns for targeted proteomics. Journal of Chromatography A.  ISSN 0021-9673.  1498, s 111- 119 . doi: 10.1016/j.chroma.2017.03.043
• Brandtzaeg, Ole Kristian; Røen, Bent Tore; Enger, Siri; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Multichannel open tubular enzyme reactor online coupled with mass spectrometry for detecting ricin. Analytical Chemistry.  ISSN 0003-2700.  89(17), s 8667- 8673 . doi: 10.1021/acs.analchem.7b02590
• Comninos, Alexander N; Wall, Matthew B; Demetriou, Lysia; Shah, Amar J; Clarke, Sophie A; Narayanaswamy, Shakunthala; Nesbitt, Alexander; Izzi-Engbeaya, Chioma; Prague, Julia K; Abbara, Ali; Ratnasabapathy, Risheka; Salem, Victoria; Nijher, Gurjinder M; Jayasena, Channa N; Tanner, Mark; Bassett, Paul; Mehta, Amrish; Rabiner, Eugenii A; Hönigsperger, Christoph; da Silva, Meire Ribeiro; Brandtzaeg, Ole Kristian; Lundanes, Elsa; Wilson, Steven Ray Haakon; Brown, Rachel C; Thomas, Sarah A; Bloom, Stephen R & Dhillo, Waljit S (2017). Kisspeptin modulates sexual and emotional brain processing in humans. Journal of Clinical Investigation.  ISSN 0021-9738.  127(2), s 709- 719 . doi: 10.1172/JCI89519
• da Silva, Meire Ribeiro; Brandtzaeg, Ole Kristian; Vehus, Tore; Lancas, Fernando Mauro; Wilson, Steven Ray Haakon & Lundanes, Elsa (2017). An automated and self-cleaning nano liquid chromatography mass spectrometry platform featuring an open tubular multi-hole crystal fiber solid phase extraction column and an open tubular separation column. Journal of Chromatography A.  ISSN 0021-9673.  1518, s 104- 110 . doi: 10.1016/j.chroma.2017.08.071
• Gregus, Michael; Røberg-Larsen, Hanne; Lundanes, Elsa; Foret, Frantisek; Kubán, Petr & Wilson, Steven Ray Haakon (2017). Non-aqueous capillary electrophoretic separation of cholesterol and 25-hydroxycholesterol after derivatization with Girard P reagent. Chemistry and Physics of Lipids.  ISSN 0009-3084.  207, s 87- 91 . doi: 10.1016/j.chemphyslip.2017.05.014
• Gundersen, Cathrine Brecke; Zhu, Liang; Lindahl, Sofia; Wang, Shiyu; Wilson, Steven Ray Haakon & Lundanes, Elsa (2017). LC-MS/MS Method for Simultaneous Determination of Monoethanol- and Dimethylnitramine in Aqueous Soil Extracts. Chromatographia.  ISSN 0009-5893.  80(9), s 1475- 1481 . doi: 10.1007/s10337-017-3355-6
• Røberg-Larsen, Hanne; Abele, Silvija; Demir, Deniz; Dzabijeva, Diana; Amundsen, Sunniva Furre; Wilson, Steven Ray Haakon; Bartkevics, Vadims & Lundanes, Elsa (2017). Rugged Large Volume Injection for Sensitive Capillary LC-MS Environmental Monitoring. Frontiers in Chemistry.  ISSN 2296-2646.  AUG(2017) . doi: 10.3389/fchem.2017.00062 Full text in Research Archive.
• Røberg-Larsen, Hanne; Lund, Kaja; Sæterdal, Kristina Erikstad; Solheim, Stian; Vehus, Tore; Solberg, Nina; Krauss, Stefan; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Mass spectrometric detection of 27-hydroxycholesterol in breast cancer exosomes. Journal of Steroid Biochemistry and Molecular Biology.  ISSN 0960-0760.  169, s 22- 28 . doi: 10.1016/j.jsbmb.2016.02.006
• Skjærvø, Øystein; Brandtzaeg, Ole Kristian; Lausund, Kristian Blindheim; Pabst, Oliver; Martinsen, Ørjan Grøttem; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Exploring bioimpendance instrumentation for the characterization of open tubular liquid chromatography columns. Journal of Chromatography A.  ISSN 0021-9673.  1534, s 195- 200 . doi: 10.1016/j.chroma.2017.12.060
• Ulanova, Lilia; Pinheiro, Marina; Vibe, Carina Beatrice; Nunes, Clàudia; Misaghian, Dorna; Wilson, Steven Ray Haakon; Zhu, Kaizheng; Fenaroli, Federico; Winther-Larsen, Hanne Cecilie; Reis, Saletta & Griffiths, Gareth (2017). Treatment of Francisella infections via PLGA-and lipid-based nanoparticle delivery of antibiotics in a zebrafish model. Diseases of Aquatic Organisms.  ISSN 0177-5103.  125(1), s 19- 29 . doi: 10.3354/dao03129
• Vehus, Tore; Waaler, Jo; Krauss, Stefan; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Combining HIC, SEC, and IEX with Fluorescence Polarization for Drug Target Discovery. LC GC North America.  ISSN 1527-5949.  30(5), s 232- 239 Full text in Research Archive.
• Åstrand, Ove Alexander Høgmoen; Viktorsson, Elvar Örn; Kristensen, Aleksander Lim; Ekeberg, Dag; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon; Gabrielsen, Mari; Sylte, Ingebrigt; Rustan, Arild; Thoresen, G. Hege; Rongved, Pål & Kase, Eili Tranheim (2017). Synthesis, in vitro and in vivo biological evaluation of new oxysterols as modulators of the liver X receptors. Journal of Steroid Biochemistry and Molecular Biology.  ISSN 0960-0760.  165, s 323- 330 . doi: 10.1016/j.jsbmb.2016.07.010 Full text in Research Archive.

View all works in Cristin

• Andersen, Kari Anne; Flatval, Jonas; Mrša, Ago; Berg, Henriette Engen; Chavan, Sachin Maruti; Olsbye, Unni; Wilson, Steven Ray Haakon & Lundanes, Elsa (2020). Metal organic framework material UiO-66 as a stationary phase in liquid chromatography. Show summary

  Metal organic framework materials (MOFs) are interesting candidates for applications in multiple disciplines, where chromatography is one of the fields where possible applications might exist. The porous structure and large surface area are key features that make MOFs potential candidates for future stationary phases. In the present study, the MOF UiO-66 has been investigated as a stationary phase in a nano-liquid chromatography (LC) system. UiO-66 is composed of zirconium oxide ion nodes and benzene dicarboxylate (BDC) linkers which form a face-centred cubic structure with octahedral pores (opening 6 Å, cavity 11 Å) and tetrahedral pores (opening 6 Å, cavity 7.5 Å) in a 1+2 ratio. Figure adapted from Cliffe et al., Nature communications volume 5 (2014)1. Columns with a 100 µm inner diameter have been packed in-house and tested in a simple LC-UV system for several test substances. The UiO-66 particles pack rapidly and yield columns with low back pressure. We observe reversed phase selectivity, but our experimental data also suggest other interaction mechanisms, phosphate group adsorption to zirconium oxide nodes among them.

• Andersen, Kari Anne; Lundanes, Elsa; Berg, Henriette Engen; Wilson, Steven Ray Haakon; Chavan, Sachin Maruti; Lillerud, Karl Petter & Olsbye, Unni (2020). UiO-66 metal-organic framework materials as stationary phases in liquid chromatography. Show summary

  The metal-organic frameworks (MOFs) UiO-66 and UiO-66-NH2 are composed of zirconium oxide nodes (Zr6O4(OH)4(CO2)12)and benzene-1,4-dicarboxylate (BDC) linkers for UiO-66, and the amino-functionalised linker 2-amino-benzene-1,4-dicarboxylate (ABDC) for UiO-66-NH2. UiO-66 and UiO-66-NH2 have large surface areas and a defined pore size and are thus attractive materials for chromatographic separations. The structure of these MOFs also meansseveral retention mechanisms could influence the separation simultaneously, making the retention of functionalised organic molecules on these materials challenging to predict. Hence, the aim of this thesiswas to investigate the chromatography of selected compounds on UiO-66 and UiO-66-NH2 in liquid chromatography (LC), to learn more about the potential applications of these UiO materials.UiO-66 and UiO-66-NH2 particles were packed into 100 μm inner diameter (ID) capillaries, and a simple LC-UV system was used for testing. Both materials rapidly (<30 min) packed into columns of circa 10 cm and provided pressures < 300 bar when used in LC systems. The efficiency (plate height) of the UiO-66-and UiO-66-NH2-columnswerefound to be 18 μm and 199 μm, respectively, using uracil asa modelanalyte. However, phosphate-containing analytes were found to have severe tailing. With an aqueous mobile phase, the retention of a selection of small hydrophilic molecules was found to increase with decreasing organic component in the mobile phase. The flow rate was also found to affect retention factors, with larger retention factors for lower flowrates. The effect of temperature in the range 25–55°C appeared to follow the expected trend of decreasing retention with increasing temperature for the chosen model substances, benzene, ethylbenzene and butylbenzene.Pore volume accessibility studies wereinconclusive. However, observations made strongly suggest that benzene, ethylbenzene, propylbenzene, butylbenzene, naphthalene and phenanthrene were able to access the pores of UiO-66.In conclusion, the UiO-66 MOF materials have chromatographic properties different from thoseof the more common LC separation materials, notably in the ability to retain small polar molecules. However, more research is needed to better understand the retention mechanisms of the materials and to single out application areas for these materials in LC.

• Berg, Henriette Engen; Halldórsson, Skarphéðinn; Thiede, Bernd; Vik-Mo, Einar O.; Lundanes, Elsa & Wilson, Steven Ray Haakon (2020). Micro pillar array columns for comprehensive proteomics. Show summary

  Pillar array nano liquid chromatography columns can potentially replace packed nano liquid chromatography (nLC) columns for analyses of complex samples due to high chromatographic performance and low backpressure. In our study, pillar array columns are used for the detection of potential biomarkers in Glioblastoma Multiforme (GBM), which is the most aggressive form of brain cancer in adults. Pillar array columns consist of several micropillars etched into silicon wafers using lithographic micromachining. The interest of these columns in nLC format has increased as an option to packed nLC columns as the chromatographic efficiency could outperform nLC columns packed with sub-2 µm particles while maintaining the sensitivity [1]. In contrast to packed columns, where eddy dispersion is the main source of band broadening due to irregular flow paths between the particles, the perfectly ordered pillars in pillar array columns eliminate the eddy dispersion to a minimum. The low backpressure of pillar array columns compared to that of packed columns also permits the use of exceptionally long columns, increasing the chromatographic performance further. Using pillar array columns, plate numbers over 1 million and reduced plate heights (h) even <1 µm have been achieved [2-4]. High chromatographic performance is especially important in comprehensive proteomics. Pre-fractionation (using e.g. gel electrophoresis) is often added in proteomic protocols to increase the throughput [5]. However, performing pre-fractionation can be time-consuming in addition to the highly increased instrument time required. Recently, a micro-Chip pillar array nLC column (µPACTM) compatible with standard nLC instrumentation has been commercialized (Figure 1) [6]. Our question was; could the use of µPAC nLC columns reduce the need for pre-fractionation in comprehensive proteomics of GBM biopsies?

• Berg, Henriette Engen; Hvinden, Ingvild Comfort; Sachse, Daniel; Skaga, Erlend; Lundanes, Elsa; Sandberg, Cecilie; Vik-Mo, Einar O.; Rise, Frode & Wilson, Steven Ray Haakon (2020). Observation of brain cancer cells’ molecular responses to chemotherapy.
• Bergh, Marianne Skov-Skov; Øiestad, Åse Marit Leere; Bogen, Inger Lise & Wilson, Steven Ray Haakon (2020). Fentanyl Analogs: Development of Bioanalytical Methods for Forensic Case Work and Studies of Metabolism and Pharmacodynamic Effects. Series of dissertations submitted to the Faculty of Mathematics and Natural Sciences, University of Oslo.. 2287.
• Boger, Ida Caroline Sneis; Nerem, Elisabeth; Skottvoll, Frøydis Sved; Harrison, Sean; Sullivan, Gareth; Lundanes, Elsa; Bogen, Inger Lise & Wilson, Steven Ray Haakon (2020). Determination of liver organoid-induced drug metabolites using liquid chromatography-mass spectrometry (LC-MS)”. Show summary

  Liver organoids are three-dimensional tissue models typically derived from adult and human induced pluripotent stem cells. They are intended to e.g. represent the physiological functions of a patient´s liver [1]. The liver is the main metabolizing organ in the human body [2]; thus, an important application of liver organoids is to map drug metabolism in vitro as liver organoids can be more patient-specific compared to traditional biomaterials, e.g. human liver microsomes (HLM). The aim of this study is to explore liver organoid drug metabolism in vitro using liquid chromatography-mass spectrometry (LC-MS). To establish a standardized conventional approach for metabolism studies for later comparison with organoids, heroin metabolism studies in HLMs and S9 fraction were carried out. Quantification of model substance heroin and its well-known metabolites, 6-Monoacetylmorphine (6-MAM) and morphine, was done using UHPLC-MS/MS. The heroin metabolism method was miniaturized to make it transferable to the small organoid samples. This was done by decreasing the microsome amount as much as possible while still observing heroin metabolism (HLM amount decreased from 0.2 mg to 0.01 mg; Heroin concentration was decreased from 10 µM to 0.1 µM). This miniaturized experiment was also transferred to S9 fraction Preliminary experiments with organoids showed that the organoids metabolize heroin to 6-MAM and morphine, as well as phase II biotransformation metabolites morphine-3-glucuronide and morphine-6-glucuronide. These results show that the studied organoids have metabolizing properties. Taken together, LC-MS can be a valuable tool for studying metabolism properties of organoids.

• Boger, Ida Caroline Sneis; Wilson, Steven Ray Haakon; Skottvoll, Frøydis Sved; Lundanes, Elsa & Bogen, Inger Lise (2020). Determination of liver organoid- and spheroid induced drug metabolites using liquid chromatography-mass spectrometry. Show summary

  The liver is the main metabolizing organ in the human body, and several in vitro models have been developed to resemble hepatic drug metabolism. However, commonly used in vitro models such as human liver microsomes (HLMs), S9 fraction, and hepatocytes lack the complexity of the corresponding in vivo tissue, which limits the in vivo resemblance. Liver organoids are three-dimensional tissue models typically derived from induced pluripotent stem cells (iPSCs) and are intended to recapitulate physiological functions of the human liver. Liver spheroids are similar 3D culture systems but lack the multicellularity that characterizes the organoids. Few studies have investigated drug metabolism in liver organoids- and spheroids. The aim of this study was, therefore, to explore liver organoid- and spheroid drug metabolism using liquid chromatography-mass spectrometry (LC-MS) as the liver organoids- and spheroids can be more representative to the in vivo situation. Additionally, an LC-MS method was to be established at the Department of Chemistry for drug metabolite detection. Drug incubated samples were analyzed using a validated LC-MS method at the Department of Forensic Sciences or by the LC-MS method established at the Department of Chemistry. Heroin was chosen as the model drug, and heroin metabolism studies were initially carried out in HLMs to establish a standardized conventional approach for later drug metabolism studies in organoids and spheroids. The concentrations of heroin and its phase I metabolites, 6-monoacetylmorphine (6-MAM) and morphine were measured over time (20 minutes). The heroin metabolism method was downscaled by decreasing the HLM concentration (from 2 mg/mL to 0.1 mg/mL) and the heroin concentration (from 1 μM to 0.1 μM). The downscaled method was applied to the S9 fraction and later executed with the addition of exogenous cofactors to look for the phase II metabolites morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G). With the addition of cofactors, M3G could be quantified in the S9 fraction. Preliminary heroin metabolism studies in iPSC derived liver organoids showed that the organoids metabolized heroin (1 μM) to 6-MAM and morphine. The phase II metabolites M3G and M6G were also identified but below the limit of quantification (LOQ). Both the phase I- and phase II metabolites were detected above LOQ in liver spheroid samples after incubation in 10 M heroin. To sum up, the detection of heroin drug metabolites using LC-MS showed that the liver organoids- and spheroids had heroin metabolizing properties. Additionally, LC-MS proved to be a valuable tool for detection of liver organoid induced drug metabolites.

• Flatval, Jonas; Berg, Henriette Engen; Lillerud, Karl Petter; Olsbye, Unni; Nielsen, Claus Jørgen; Wilson, Steven Ray Haakon & Lundanes, Elsa (2020). Retention of nitramines on UiO-66 ZrMOF materials and silica-based reversed phase materials.
• Flatval, Jonas; Lundanes, Elsa; Wilson, Steven Ray Haakon; Olsbye, Unni; Nielsen, Claus Jørgen & Berg, Henriette Engen (2020). Retention of nitramines on silica-based reversed phase materials and UiO-66 MOF materials. Show summary

  Capturing and storing of CO2 can reduce the emissions of CO2 in the atmosphere. However, such facilities may emit amines that can be transformed into the potentially carcinogenic nitramines dimethylnitramine (DMA-NO2), monoethanolnitramine (MEA-NO2) and monomethylnitramine (MMA-NO2) which may be released into the environment. Presently, methods for trace determination of these compounds are lacking. The goal of the study was to find a material that can give sufficient retention to provide an enrichment of the nitramines before liquid chromatography electrospray ionization-mass spectrometry determination. The retention of the nitramines was examined on various C18 materials and the metal-organic frameworks UiO-66 and UiO-66 1,4-benzenedicarboxylate-NH2 (UiO-66-NH2), which were packed in 100 µm inner diameter columns. Of the tested materials, UiO-66-NH2 showed the highest retention factors for the nitramines. UiO-66-NH2 with 0.1% formic acid adjusted to pH 7 as mobile phase gave the best compromise of retention factors for DMA-NO2 and MEA-NO2, as the intended liquid chromatography-mass spectrometry method was evaluated for DMA-NO2 and MEA-NO2 only. MMA-NO2 had the highest retention factor on UiO-66-NH2 with 0.05% dibutylamine in the mobile phase. In conclusion, the retention factors achieved were in general higher on the UiO-66 materials than on the C18 materials. However, the retention obtained is probably not large enough to give limit of quantification down to 4 ng/L, albeit, not tested, due to extraordinary circumstances.

• Haugan, Hege Sofie; Elgstøen, Katja B. Prestø; Skogvold, Hanne Bendiksen & Wilson, Steven Ray Haakon (2020). Investigating the Lipid Coverage of the Dried Blood Spot Metabolome using Liquid Chromatography – Mass Spectrometry for Global Metabolomics. Show summary

  Using global metabolomics for the diagnosis of inborn errors of metabolism canprovide greater insight into the diseased metabolic pathway, but it isimportant to understand thelimitations of the analytical techniquein terms of which metabolites are covered by it.In this project, the lipid coverage of a global metabolomics method usingliquid chromatography-electrospray ionization-mass spectrometryand dried blood spots wasinvestigated.The lipid coverage andpossible expansion of the coverage was investigated by extraction of commercially available lipid standards,metabolite standards,and dried blood spots,usinga selection ofsolvents in different ratioswith water: methanol, acetonitrile, 1-propanol, iso-propanol, 1-butanol, or methyl-tert-butyl-ether.The methods ability to detect physiological change in themetabolome and lipidome was also investigated.Quality control sampleswereimplemented to assessthe precision of the acquired data.Increasing the amount of organic solvent used in the extraction solution was found to increase lipid coverage, butitwasnot greatly affected by type of organic solventused. The method detectedchanges in the metabolic profile of seven healthy volunteers participating in a high intensityexercise. Several metabolites and lipids were identified and affected by the exercise.Pooled quality controls were implemented in the global metabolomics workflow and showed that the data acquired had high precision. A series of diluted pooled quality controls showed that there was a linear relationship between signal intensity and metabolite concentration, with R2>0.98.This method will bea usefulfor researching differences in metabolic profiles, but also as a supplementarydiagnostic toolfor inborn errors of metabolism.

• Hermansen, Astrid; Skottvoll, Frøydis Sved; Olsen, Christine; Desmet, Gert; Wilson, Steven Ray Haakon & Lundanes, Elsa (2020). Preparation of porous layer open tubular columns for sensitive proteomics. Show summary

  The newly introduces 3D liver cellular cluster model, known as liver organoids, is a promising tool to probe disease and human biology. The composition and function of liver organoids is not yet fully understood, calling for sensitive proteomic analysis due to limited sample size and availability. The use of narrow inner diameter (ID) liquid chromatography (LC) separation columns is beneficial for increased sensitivity. A relevant column format to explore is the porous layer open tubular (PLOT), which with narrow ID and low backpressure permit the use of longer columns for higher separation power. The goal of this work is to prepare silica-based C18 PLOT columns with narrow ID (10 and 5 µm) suitable for peptide and intact protein separation and find the optimal conditions for proteomics of liver organoids and their media with mass spectrometry detection. Initial analysis of HeLa cell digest using a PLOT column (C18, 10 µm ID x 56 cm) made by Hara et al., has been performed. HeLa cell digest has also been analyzed using a commercially available packed column (C18, 75 µm x 15 cm) for comparison. Two approaches for the preparation of silica-based C18 PLOT columns with narrow ID were explored, based on methods established by Hara et al. and Vehus et al., respectively. We experienced challenges with regards to clogging of the fused silica capillaries during the formation of the porous silica layer when using the method established by Vehus et al., thus only the method of Hara et al. was further explored. Scanning electron micrographs indicate that a porous layer was present with both preparation methods.

• Hermansen, Astrid; Skottvoll, Frøydis Sved; Wilson, Steven Ray Haakon & Lundanes, Elsa (2020). Development and implementation of open tubular columns for high-sensitivity liquid chromatography-mass spectrometry proteomics of liver organoids. Show summary

  The three-dimensional liver cellular cluster model, known as liver organoids, is a promising tool to better mimic human physiology for drug development. The composition and function of liver organoids arenot yet fully understood, calling for sensitive proteomic analysis due to limited sample size and availability. The use of narrow inner diameter (ID) liquid chromatography (LC) separation columns is beneficial for increased sensitivity. A relevant column format to explore is the porous layer open tubular (PLOT), which with narrow ID and low backpressure permit the use of longer columns for higher separation power.Proteomic analysis of organoids using PLOT at column format was yet to be explored.The goal of this work was to prepare silica-based PLOT columns functionalized with octadecyl (C18) with narrow ID (10 and 5 μm) suitable for sensitive proteomic analysis of liver organoids with mass spectrometry detection. Two approaches for the preparation of silica-based PLOT columns functionalized with C18 were explored and successfully prepared. Scanning electron micrographs could indicate that a porous layer was present with both preparation methods; however, one method had challenges regarding clogging during the preparation of the columns. A peak capacity of 127 was obtained with an in-house made PLOT column (10μm x 115.2 cm) when analysing 20 ng HeLa tryptic digest using a 240-minute linear gradient (2.7-15% acetonitrile in water + 0.1% formic acid).Proteins related to the liver metabolic pathways were identified from the analysis of liver organoid fractions using one in-house made PLOT column.Thus, indicating that silica-based C18 PLOT columns constitute a promising tool for the bioanalysis of limited samples.

• Mrša, Ago; Skottvoll, Frøydis Sved; Harrison, Sean; Lundanes, Elsa; Sullivan, Gareth & Wilson, Steven Ray Haakon (2020). Miniaturized liquid chromatography systems for small molecule analysis of organoids. Show summary

  Liver organoids are three-dimensional tissue models (typically in the millimetre scale or below) derived from human-induced pluripotent stem cells [1]. They are intended to e.g. represent the physiological functions of a patient’s liver. The liver is the main metabolizing organ in the human body [2]; thus, an important application of liver organoids is to map drug metabolism in vitro, as liver organoids can be more patient-specific compared to traditional biomaterials. The aim of the study is to develop miniaturized liquid chromatography (LC) systems capable of analyzing very small samples, e.g secretion of liver organoids. Emphasis is placed on the determination of small molecules, i.e metabolites. Due to the small size of organoids, there are significant challenges in performing sensitive analysis of the organoid itself and it's minute secretion. Hence a nanoLC system has been explored, capable of sensitive analysis of model substance heroin and its well-known metabolites morphine and 6-Monoacetylmorphine (6-MAM). Using a 50 µm x 5 cm C18 pre-column and a 50 µm x 12 cm C18 analytical column, coupled up with a triple quadrupole mass spectrometer (TQMS) with electrospray ionization, a limit of detection of 0.7 fg was achieved for heroin. Further method development was done using commercially packed C18 columns typically used in proteomics. Separation and detection of heroin, morphine and 6-MaM were achieved in less than 8 minutes and with detection limits in the sub fg area. The method was successfully transferred to liver organoid samples that had been incubated with 1.5 µM heroin for 1 hour, cleaned using electromembrane extraction and further diluted 1:1000. Deuterated internal standards were implemented into the method for future work with organoid samples and the next step is to map the metabolism of liver organoids incubated within various time frames. Taken together, nanoLC-MS proves to be a viable tool for analysis of small molecules and the achievd results are promising for further work with organoid samples.

• Mrša, Ago & Wilson, Steven Ray Haakon (2020). Miniaturized liquid chromatography systems for small molecule analysis of organoids.
• Olsen, Christine; Scholz, Hanne; Wilson, Steven Ray Haakon & Lundanes, Elsa (2020). Towards online reduction and protein digestion of secreted proteins from pancreatic islets of Langerhans related to diabetes mellitus type I. Show summary

  What is diabetes mellitus type 1, and how can liquid chromatography assist in the search for a cure? The human pancreas is involved in control of metabolism and energy consumption by both an exocrine and an endocrine function. In the exocrine tissue, a multitude of digestive enzymes are produced and secreted into the small intestine to assist in breaking down proteins, fats and carbohydrates. The endocrine tissue, islets of Langerhans (5% of the total pancreatic mass) are responsible for regulation of the blood glucose levels. The vital metabolic hormone insulin is synthesized in one of five major cell types in the islets, called β-cells [1]. However, there are two types of chronic diseases related to insulin deficiency; an autoimmune disease causes selectively destruction of the β-cells in the islets or the body cannot effectively use the produced insulin, called diabetes mellitus type 1 and type 2, respectively. Diabetes mellitus type 1 or type 2 have been diagnosed in 250 000 Norwegians (approx. 4.7% of the Norwegian population), where 28 000 have been diagnosed with type 1 diabetes mellitus (0.5%) [2]. The most common treatment is exogenous insulin therapy. When insulin was discovered in 1920s the mortality of diabetes type 1 was reduced from a guaranteed fatal diagnosis to a manageable condition with a normal lifespan within the 1950s. However, secondary complications due to diabetic nephropathy, retinopathy and neuropathy led to a renewed interest in β-cell replacement therapy. Progress in β-cell replacement with either pancreas or islet transplantation has led to impressive 1- and 5-year insulin independence rates of approximately 85% and 50 %, respectively [3]. To assist in further progress in β-cell replacement therapy, liquid chromatography with mass spectrometry detection (LC-MS) of the secreted proteins from the pancreas and the islets will aid in the understanding of the treatment of diabetes mellitus type 1. In this work, we seek to enable fully online determination of proteins (in a bottom-up approach) by combining reduction and protein digestion upstream LC-MS (Figure 1). Online protein digestion has been successfully executed with immobilized enzyme reactors (IMERs) in different formats [4-6], but most of the samples have been reduced and alkylated prior to online digestion. We hope to enable real-time determination of proteins with disulfide bonds (such as insulin) by incorporation of a photochemical system for rapid reduction of disulfide bonds [7].

• Røberg-Larsen, Hanne; Websdale, Alex; Solheim, Stian Kjønnås; Sande, Maria Therese; Hutchinson, Samantha A; Lundanes, Elsa; Wilson, Steven Ray Haakon & Thorne, James L (2020). Fast LC-MS for determination of oxysterols in breast cancer tumours.
• Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2020). Low-flow Rate Separations of Lipids, In William Griffiths & Yuqing Wang (ed.),  Lipidomics: Current and Emerging Techniques.  Royal Society of Chemistry (RSC) Publishing.  ISBN 978-1-78801-160-0.  s 49 - 73
• Schüller, Maria; Skottvoll, Frøydis Sved; Lundanes, Elsa & Wilson, Steven Ray Haakon (2020). Bottom-up proteomics of biomarkers to investigate drug-induced hepatotoxicity in human liver organoids as part of preclinical development. Show summary

  Drug-induced liver injury (DILI) is an ongoing issue in the development of drugs. It is a major cause of drug withdrawal during development, as well as being the leading cause for post-authorization withdrawal. DILI is commonly discovered in later stages of clinical trials and poses a risk to trial subjects involved in the study. European Medicines Agency (EMEA) provide guidelines for non-clinical assessment of DILI, however regulatory authorities do not require this assessment for the approval of the Investigational New Drug (IND) application for testing in human subjects. This is possibly due to lack of research and validity of current DILI practices which, at worst, could hinder promising therapeutics to enter the market. In recent years, the use of 3D liver cellular clusters, commonly known as liver organoids, has alongside other advances, emerged as a promising tool to supplement the nonclinical assessment of drug candidates. Liver organoids closely resemble human liver physiology and emulate basic liver function. In combination with well-established clinical protein biomarkers, they might revolutionize the drug industry and strengthen patient safety. Organoids and organoid medium are complex in nature and limited in size and availability. Implementing nanoLC for analytical method development for liver organoid analysis will achieve improved sensitivity and therefore capture the detection of minor fluctuations in protein biomarkers upon drug exposure. The goal of this work is to develop a targeted proteomics method for the absolute quantification of alanine aminotransferase (ALT) in organoid medium based on unique peptide MRM transitions with nanoLC-tandem MS. Pilot experiments using human serum albumin (HSA) have been conducted to create a basic outline of experiments which are reasonable to conduct when approaching the main goal. HSA is a readily available and relatively affordable pure protein source, well suited for preliminary testing. The optimal linear gradient for eluting HSA peptides within 48 min was 1% - 15% mobile phase component B (0.1% formic acid and 10% water in acetonitrile). A positive correlation between SSRCalc values (hydrophobicity factor) and elution time has been established and can be implemented in developing the optimal gradient for peptides of ALT. The most abundant peptides have been optimized with respect to collision energy (CE) using a scheduled MRM approach. Regression analysis was performed to establish a relationship between CE and precursor m/z, which can be implemented in ALT analysis. HSA was spiked into HeLa cell lysate to investigate matrix suppression effects, as well as to investigate the effects of gradient length on ion suppression. Planned experiments will revolve around selecting signature peptides for ALT, quantify ALT with suitable quantification methods and measure ALT levels in organoid medium upon exposure to known hepatotoxins.

• Schüller, Maria; Wilson, Steven Ray Haakon; Lundanes, Elsa & Skottvoll, Frøydis Sved (2020). A targeted proteomics approach for the investigation of drug-induced liver injury in hepatic organoids using nano liquid chromatography mass spectrometry. Show summary

  Late-stage drug attrition and post-market withdrawals attributed to drug-induced liver injury (DILI) has been a prevailing concern for the pharmaceutical industry. The current gaps in preclinical safety assessment impede the termination of DILI drug candidates to earlier stages of drug development, with the consequence of possibly exposing patients to hepatotoxic agents. Recent advances in liver-emulating models, with the development of hepatic organoids, seem promising in filling these gaps. The purpose of this study was to look into opportunities for the advancement of the preclinical safety assessment of the hepatic system. This was approached by combining the liver-emulating power of hepatic organoids with the well-studied protein biomarker alanine aminotransferase isoform 1 (ALT1) to investigate their compatibility with nano liquid chromatography mass spectrometry (nanoLC-MS). A targeted proteomics approach for the absolute quantification of ALT1 was developed by a thorough assessment of signature peptide candidates and corresponding multiple reaction monitoring (MRM) transitions. NanoLC-MS platform optimization was performed to maximize detection sensitivity and reliability. From empirical nanoLC-MS platform optimization, the greatest improvement was achieved from collision energy optimization, with an increase of mean peptide peak area of 22%. ALT1 peptide assessment showed that the peptide LLVAGEGHTR with the MRM fragments y8+ and y7+ were best suited to infer the presence of ALT1 in absolute quantification. The finalized method was applied for the investigation of drug-induced liver injury in hepatic organoids, where acetaminophen was used as the model drug. This work has shown that ALT1 quantification with a nanoLC-MS platform is feasible and has great potential to support preclinical DILI detection. Further studies must be conducted to give reliable statements on the applicability of the method for drug-induced ALT1 release in hepatic organoids.

• Skottvoll, Frøydis Sved; Harrison, Sean; Hansen, Frederik; Pedersen-Bjergaard, Stig; Sullivan, Gareth; Bogen, Inger Lise; Lundanes, Elsa & Wilson, Steven Ray Haakon (2020). Liver mini-organ analyses using liquid chromatography mass spectrometry. Show summary

  Organoids (self-organized multi-cellular tissue derived from e.g. patient-derived stem cells) are emerging in vitro 3D representations of human organ functionality, predicted to have a game-changing impact on drug discovery, disease modeling and personalized medicine [1]. Integrating the organoids in a microfluidic chip (i.e. organoids-on-a-chip) would allow for controllable perfusion of nutrients and manipulation of the organoids by the addition of e.g. drugs [2]. Coupling the organoid-on-a-chip online to liquid chromatography mass spectrometry (LC-MS) would enable real-time measurements of the biochemical processes that occur on-chip. However, limited sample sizes, complex matrices and the presence of low abundant analytes also create analytical challenges [3]. Moreover, the organoids are still at the developmental stage, thus fundamental questions on the functionality of the developed organoids remain to be answered. Liver organoid functionality was assessed by protein characterization of adult liver tissue and liver organoids using nanoLC-MS/MS. The proteins identified from the liver organoids covered 70-84% (1003 proteins) of the liver protein expression from adult liver tissue derived from five patients. The proteins that were shared between the adult liver tissue and liver organoids could also relate to central liver pathways (e.g. amino acid biosynthesis and glycolysis). The sample preparation technique electromembrane extraction (i.e. electrophoretic separation across an oil membrane, EME) has shown to have on-chip analytical potential [4] and was thus used in the study of heroin drug metabolism in liver organoids. Multi-well EME (100 µL solutions) allowed for simple and repeatable monitoring of heroin metabolism kinetics without interference from cell medium components (e.g. albumin). The EME extracts were also compatible with different analytical approaches (LC-MS, nanoLC-MS, capillary electrophoresis-UV). Hence, the hyphenation of EME with nanoLC-MS would be a natural next step. References 1. Method of the Year 2017: Organoids. Nature Methods. Vol. 15 (2018) 1-1. 2. Park, S.E., A. Georgescu, and D. Huh, Organoids-on-a-chip. Science. Vol. 364 (2019) 960. 3. Lin, A., F. Sved Skottvoll, S. Rayner, S. Pedersen-Bjergaard, G. Sullivan, S. Krauss, S. Ray Wilson, and S. Harrison, 3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry. ELECTROPHORESIS. Vol. 0 (2019). 4. Hansen, F.A., D. Sticker, J.P. Kutter, N.J. Petersen, and S. Pedersen-Bjergaard, Nanoliter-Scale Electromembrane Extraction and Enrichment in a Microfluidic Chip. Analytical Chemistry. Vol. 90 (2018) 9322-9329.

• Wilson, Steven Ray Haakon & Gundersen, Lise-Lotte (2020). Fra Tippeligaen til Champions League. Aftenposten (morgenutg. : trykt utg.).  ISSN 0804-3116.
• Hermansen, Astrid; Schüller, Maria; Skottvoll, Frøydis Sved; Hara, T; Desmet, Gert; Lundanes, Elsa & Wilson, Steven Ray Haakon (2019). Protein Analysis of Liver Oganoids with Minaturized LC-MS: Nano and PLOT Format. Show summary

  In recent years, 3D liver cellular clusters, commonly known as liver organoids, have emerged as promising tools to replace 2D cell cultures and animal testing in the development of drugs and the understanding of diseases. The composition and function of liver organoids is yet not fully understood, calling for proteomic analysis. Organoids and the medium in which they secrete into are complex in nature and limited in size and availability; therefore, the use of narrow inner diameter separation columns is beneficial to achieve better sensitivity. Conventional packed columns can have limitations regarding sensitivity and separation power. Consequently, the employment of other column formats can be explored. One such format is the porous layer open tubular (PLOT) column for liquid chromatography (LC), which have low backpressures and smaller inner diameters, permitting longer columns for expanding separation power. The goal of this work is to find the optimal conditions for bottom-up proteomics of liver organoids and their media, comparing reversed-phase LC with commercially packed columns and PLOT columns with 1 / 2 mass spectrometry (MS) detection. Initial analysis of liver organoids with commercially packed columns and conventional run times revealed liver traits as phase 1 and phase 2 metabolism enzymes (aldehyde dehydrogenase mitochondrial, cytochrome P450 1B1 and glutathione S-transferase Mu 3, UDP-glucuronosyltransferase 1, catechol Omethyltransferase), proteins related to nitrogen and glutamate- (glutamate dehydrogenase) and amino acid metabolism (aspartate aminotransferase). Regarding 75 µm inner diameter/15 cm long packed columns, there was a significant difference (α = 0.05) in protein ID numbers when comparing 2 h with 4 and 8 h linear gradients from 2.7-15.3% ACN. The 4 h gradient did not significantly differ from an 8 h gradient, implying that the protein ID numbers capacity was reached at 4 h. We have now proceeded to explore the possibilities of PLOT LC-MS for organoid proteomics, using a 10 µm inner diameter/56 cm long column.

• Kogler, Stian & Wilson, Steven Ray Haakon (2019). On-line “Organ-in-a-Column” Liquid Chromatography Electrospray Ionization Mass Spectrometry for Drug Metabolism Studies. Show summary

  Organoids and organ-on-a-chip (OoC) are human-induced pluripotent stem cell-derived miniaturized organs that recapitulate organ specific functions. They open up a plethora of opportunities to change the way we test drugs, research diseases, and treat patients. In this context, liquid chromatography (LC) and mass spectrometry (MS) will have to be used, e.g. for monitoring drug metabolism, toxicity screens and organ function. On-line coupling of these technologies would allow for automatized, high-throughput systems. By downsizing the LC-MS part, low abundant analytes can also be determined. In this study, an on-line sampling and analysis system for OoC-technology has been developed. The system features hepatic organoids in a flow-through tube with continuous medium flow (organ-in-a-column, OiC), a two-loop valve system for sampling, reverse phase liquid chromatography (LC) analysis, and electrospray ionization mass spectrometry (ESI-MS). Phase 1 metabolism of heroin to its metabolite morphine was successfully measured on-line with the described system. Live/dead double staining revealed the potential of long-term cultivation of organoids in the system. In parallel, tin oxide coated fused silica nanoelectrospray ionization (nanoESI) emitters suitable for the most narrow bore chromatographic columns have been developed with HF etching and an atomic layer deposition (ALD) approach. The emitters have been tested against commercially available stainless steel (SS) emitters by injections of heroin and showed similar signal-to-noise ratios. These emitters will find a use in a future miniaturized version of the system described herein. In summary, a proof-of-concept system for on-line measurement of heroin metabolites has successfully been developed for a simple OoC-like device. NanoESI emitters for the most narrow columns have also been developed with a conductive ALD-coating of SnO2.

• Mrša, Ago; Boger, Ida Caroline Sneis; Nerem, Elisabeth; Skottvoll, Frøydis Sved; Harrison, Sean; Lundanes, Elsa; Sullivan, Gareth; Bogen, Inger Lise & Wilson, Steven Ray Haakon (2019). Studying organoid drug metabolism using UHPLC/nanoLC-MS. Show summary

  Liver organoids are three-dimensional tissue models typically derived from adult and human induced pluripotent stem cells. They are intended to e.g. represent the physiological functions of a patient´s liver [1]. The liver is the main metabolizing organ in the human body [2]; thus, an important application of liver organoids is to map drug metabolism in vitro as liver organoids can be more patient-specific compared to traditional biomaterials, e.g. human liver microsomes (HLM). The aim of this study is to explore liver organoid drug metabolism in vitro using liquid chromatography-mass spectrometry (LC-MS). To establish a standardized conventional approach for metabolism studies for later comparison with organoids, heroin metabolism studies in HLMs were carried out. Quantification of model substance heroin and its well-known metabolites, 6-Monoacetylmorphine (6-MAM) and morphine, was done using UHPLC-MS/MS. The heroin metabolism method was miniaturized to make it transferable to the small organoid samples. This was done by decreasing the microsome amount as much as possible while still observing heroin metabolism (HLM amount decreased from 0.2 mg to 0.01 mg; Heroin concentration was decreased from 10 µM to 0.1 µM). Preliminary experiments with organoids showed that the organoids metabolize heroin to 6-MAM and morphine, as well as phase II biotransformation metabolites morphine-3-glucuronide and morphine-6-glucuronide. These results show that the studied organoids have metabolizing properties. We are also exploring sensitive nano-LC-MS (traditionally used for proteomics) for studying organoid drug metabolism. Using a 50 µm (I.D) x 5 cm C18 pre-column and a 50 µm (I.D) x 12 cm C18 analytical column, coupled up with a triple quadrupole MS (QMS) using electrospray ionization (ESI), a LoD of 0.7 fg heroin was achieved. The LoD of the nano-LC-MS method gives a good foundation for future work with detection of trace liver-organoid induced drug metabolites. Taken together, LC-MS can be a valuable tool for studying metabolism properties of organoids. [1] Eisenstein, M., Organoids: the body builders, Nature Methods, (2018) 19. [2] Brandon, E. F., Raap, C. D., Meijerman, I., Beijnen, J. H., Schellens, J. H., An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons, Toxicology and applied pharmacology, (2003) 233-246

• Olsen, Christine; Wilson, Steven Ray Haakon; Skottvoll, Frøydis Sved; Brandtzaeg, Ole Kristian; Rongved, Pål & Lundanes, Elsa (2019). Synthesis and immobilization of linked Wnt-signaling pathway inhibitor on organic monoliths as a proof-of-concept of a capti remedium ad monolitus reactor for online drug deconvolution. Show summary

  A challenge in drug discovery is the identification of the drug target, called drug deconvolution. Additionally, off-target effects are considered as one of the reasons many developed drugs fail in the clinical trials. The goal of this work was to develop a solid support, displaying low secondary interactions, for immobilization of drugs (named by author as a CRAM reactor) suitable for incorporation online liquid chromatography mass spectrometry set-ups. The hypothesis was that selective purification on the online reactor would allow identification of low abundant drug targets as a consequence of reduced handling time, contamination and loss of the sample. As a proof-of-concept, an ethylene dimethacrylate-co-vinyl azlactone (EDMA-co-VDM) monolith, prepared in 180 µm inner diameter (ID) or 250 µm ID polyimide-coated fused silica capillaries, would be immobilized with Wnt-signaling pathway inhibitor 161. The 161-immobilized CRAM reactor would then attempt to selectively trap and release a low abundant protein target, tankyrase 2 (TNKS2). The EDMA-co-VDM monolith was successfully prepared in 250 µm ID capillaries. The Wnt-inhibitor 161 was rejected based on MS characterization and LDW639, a structural analogue of Wnt-inhibitor XAV939, was successfully synthesized by the author. To improve availability of LDW639 after immobilization, a linker was attached to LDW639 during synthesis. The linked LDW639 showed 50% inhibition of the Wnt-signaling pathway at a concentration of 11 µM after 24 hours incubation in cells. The EDMA-co-VDM monolith showed secondary interactions towards proteins, but the issues were resolved by quenching the reactive VDM monomer with either monoethanolamine (MEA) or an excess of linked LDW639. Immobilization of the linked LDW639 was found to be successful based on measured UV-Vis absorbance of solutions containing LDW639 was reduced by flushing a monolith, but not by monoliths already flushed with MEA (MEA monolith). The linked LDW639-immobilized CRAM reactors and the MEA monolith were not able to trap and release TNKS1/2 from human embryonic kidney 293 cells after cell lysis with a non-denaturing buffer. Showing that the identification of the drug target from complex matrices remained a challenge, even with tailored materials.

• Riddervold, Jan Thomas & Wilson, Steven Ray Haakon (2019). Immunocapture of oxytocin followed by liquid chromatography-mass-spectrometric detection.
• Sande, Maria Therese; Wilson, Steven Ray Haakon; Lundanes, Elsa & Røberg-Larsen, Hanne (2019). Enzymatic hydrolysis with cholesterol esterase in the presence of surfactant improves efficiency for sample preparation of side-chain oxysterols in biosamples.
• Skogvold, Hanne Bendiksen; Østeby, Anja; Sandås, Elise Mørk; Rootwelt, Helge; Woldseth, Berit; Wilson, Steven Ray Haakon; Ferrante, Linda; Opdal, Siri Hauge; Rognum, Torleiv Ole; Rønning, Per Ola & Elgstøen, Katja B. Prestø (2019). Effects of storage time on the dried blood spot metabolome.
• Skottvoll, Frøydis Sved; Boger, Ida Caroline Sneis; Harrison, Sean; Nerem, Elisabeth; Bogen, Inger Lise; Sullivan, Gareth & Wilson, Steven Ray Haakon (2019). LC-MS approaches to liver organoid analyses. Show summary

  Liquid chromatography coupled to mass spectrometry (LC-MS) is an analytical tool used to separate and measure the mass (m/z) and concentration of compounds in matrices like water, blood, and tumors. Drugs, metabolites and proteins are all measurable with LC-MS. Unfortunately, the sample is diluted during chromatography, hurting the sensitivity. Thus, the limited sample volumes and low abundant compounds of organ-on-a-chip and organoids demands miniaturization of LC-MS to enable measurements of high sensitivity. By narrowing the inner diameter (ID) of the separation column, the sample dilution is vastly reduced, adding to sensitivity, e.g. enabling analysis of limited sample volumes and low abundant compounds of the organ-on-a-chip and organoids. The bioanalytical group at the Department of Chemistry (UiO) is currently developing miniaturized systems for proteins and small molecules for assessing the properties of liver organoids. These efforts will be expanded to testing islet and adipose organoids, and will also be incorporated in to on-line systems, e.g. organ on a chip systems. We believe that LC-MS and organoid analysis will be an increasingly natural partnering.

• Wilson, Steven Ray Haakon; Berg, Henriette Engen; Røberg-Larsen, Hanne & Lundanes, Elsa (2019). Hyphenations of one-dimensional capillary liquid chromatography with mass spectrometry: state-of-the-art applications, In Peter Tranchida & Luigi Mondello (ed.),  Hyphenations of Capillary Chromatography with Mass Spectrometry.  Elsevier.  ISBN 9780128096383.  Kapittel 3.3.  s 319 - 358
• Amundsen, Sunniva Furre; Wilson, Steven Ray Haakon; Berg, Henriette Engen; Røberg-Larsen, Hanne & Lundanes, Elsa (2018). Online entrapment of epitope-containing peptides from integrins using immobilized antibodies.
• Ben Hassine, Esma; Lundanes, Elsa; Wilson, Steven Ray Haakon; Vehus, Tore; Brandtzaeg, Ole Kristian & Berg, Henriette Engen (2018). Capillary poly(styrene-co-octadecene-co-divinylbenzene) monolithic trap columns for bioanalytical analysis. Show summary

  The increasing demand for faster and more efficient separations for biological samples have driven organic polymer-based monolithic columns back into the spotlight. The nature of the monolithic structure allows the use of higher flow rates without increasing the system backpressure. They are also easier to contain inside the column body, opposed to particle packed columns, which are held in place by frits. These are some of the advantages that monolithic columns offer compared to particle packed columns. Poly(styrene-co-octadecene-co-divinylbenzene) (PS-OD-DVB) monolithic columns have been prepared and characterized using the neuropeptide oxytocin, “the love hormone” as test compound. The columns, 50 µm inner diameter x 100 mm in length, were to be used as trap columns in a miniaturized liquid chromatography-mass spectrometry (LC-MS) column switching system for bottom-up proteomics analysis. The monolithic PS-OD-DVB columns were prepared in situ (inside the fused silica capillary), using a polymerization mixture consisting of an initiator (lauroyl peroxide), a crosslinker (divinylbenzene), monomers (styrene and 1-octadecene), a good porogen (N,N-dimethylformamide (DMF)) and a bad porogen (1-decanol). Polymerization time, polymerization temperature, initiator concentration, and monomer to porogen ratios were investigated in order to obtain a monolithic structure with a high surface area and good permeability. The best PS-OD-DVB monolithic column was prepared using a mixture of 1.9% lauroyl peroxide (weight percentage of monomer amount), 14.2% divinylbenzene, 15.2% monomers, 11.9% DMF and 57.5% 1-decanol. The column was polymerized for one hour at 73°C and gave a plate height of 62 µm, a backpressure of 23 bar at a flow rate of 500 nL/min with a mobile phase consisting of 20% B (acetonitrile (ACN), water, formic acid (FA), 80/20/0.1, v/v/v) and 80% A (water with 0.1% (v/v) FA) and a retention time repeatability of 2.6% (relative standard deviation). The repeatability of the columns made by various individuals was satisfactory. However, when preparing twenty-one columns by the same individual, the repeatability revealed to be questionable. Hence, further improvements in the polymerization procedure do seem necessary and might be able to enhance the repeatability of the columns.

• Berg, Henriette Engen; Skottvoll, Frøydis Sved; Bjørseth, Kamilla; Sæterdal, Kristina Erikstad; Brandtzaeg, Ole Kristian; Vehus, Tore; Hustoft, Hanne Kolsrud; Røberg-Larsen, Hanne; Thiede, Bernd; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). Development of nanoLC column technology for proteomic studies. Show summary

  Background NanoLC has emerged as a major technique in proteomics due to improved sensitivity, which enables analysis of small sample volumes and low concentrations. In our group (Bioanalytical chemistry, BACH) we focus on the development of column technology, including porous layer open tubular (PLOT) columns and self-packing of columns for proteomic applications. Methods The PLOT columns were used for both on-line digestion (enzyme reactors) and separations (10 µm ID, PS-DVB with ODS). Enzyme reactors (20 µm ID and multi-PLOT columns (~8 µm ID)) were immobilized with trypsin and Lys-C for protein digestion. We also compared self-packed columns of 50 µm ID (2.6 µm C18 core-shell particles) to a commercial column (75 µm ID). The columns were used for detection of CYP27a1 in MDA-MB-231 (breast cancer) cells, and biomarker candidates in MDA-MB-231 and glioblastoma multiforme (brain cancer) exosomes. For exosome isolation (cell culture medium), ultracentrifugation (“golden standard”) was compared to a Total Exosome Isolation Reagent from Thermo Fisher regarding protein yield and purity. Results Our PLOT columns were successfully employed for proteins, metabolites and peptides with attogram detection limits and peak capacities around 250 [1]. The enzyme reactors provided sufficient digestion in ~5 minutes compared to overnight digestions (common in off-line protocols) [2]. Furthermore, a quantitative method of the neurotoxin ricin was developed using the multi-PLOT to reduce manual sample handling [3] . The self-packed columns obtained peak capacities comparable to the commercial nanoLC column for peptide separations [4]. The self-packed columns were used for the targeted detection of CYP27A1 (potential breast cancer biomarker) in MDA-MB-231 cells. In the recent exosome study, exosomes were present and biomarker candidates were identified. However, the characterization techniques are in our hands not satisfactory. Conclusions We have successfully developed sensitive nanoLC column formats for proteomic applications.

• Berg, Henriette Sjånes; Bjørseth, Kamilla; Sæterdal, Kristina Erikstad; Smetop, Tone; Skottvoll, Frøydis Sved; Vehus, Tore; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). Evaluation of isolation methods of glioma exosomes for biomarker discovery. Show summary

  Gliomas are the most common form of brain cancer, where the sub-group glioblastoma multiforme (GBM) is the most aggressive and the most common in adults [1]. For diagnostic, prognostic, and treatment monitoring, fast and reliable analytical methods for detection of glioblastoma tumors are essential. Non-invasive monitoring of circulating biomarkers in blood (liquid biopsy) is a desirable possibility [2]. Nano LC-MS can provide the high sensitivity, selectivity and low false positive rate needed for the proteomic analysis of exosomes, which are extracellular vesicles released from the tumor and into the bloodstream. We have optimized the packing of 50 µm capillaries (2.6 µm C18 core-shell particles) with peak capacities comparable to similar commercial nano-LC columns (75 µm ID) for peptide separations of minute samples (e.g. exosomes). These columns have previously been successfully used for the targeted detection of CYP27a (a potential breast cancer biomarker) in the MDA-MB-231 cell line. In the present study, these in-house packed columns have been applied for proteins in exosomes isolated from GBM cell culture medium. The aim of this study was to compare isolation methods of GMB exosomes for biomarker discovery and study proteins related to GBM in exosomes. Ultracentrifugation (“golden standard” in exosome isolation) was compared to a Total Exosome Isolation Reagent from Invitrogen (Thermo Fisher Scientific) regarding sample volume, total protein amount, purity, and the presence of exosome markers and GBM biomarker candidates. References 1. Molina, J.R., Y. Hayashi, C. Stephens, and M.M. Georgescu, Invasive Glioblastoma Cells Acquire Stemness and Increased Akt Activation. Neoplasia. 12 (2010) 453-U37. 2. Best, M.G., N. Sol, S. Zijl, J.C. Reijneveld, P. Wesseling, and T. Wurdinger, Liquid biopsies in patients with diffuse glioma. Acta Neuropathologica. 129 (2015) 849-865.

• Brandtzaeg, Ole Kristian; Levernæs, Maren Christin Stillesby; Halvorsen, Trine Grønhaug; Reubsaet, Leon; Røen, Bent Tore; Vehus, Tore; da Silva, Meire Ribeiro; Røberg-Larsen, Hanne; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). Online sample processing systems for targeted nano liquid chromatography mass spectrometry bioanalysis.
• Brandtzaeg, Ole Kristian; Røen, Bent Tore; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). Qualitative detection of ricin using a multichannel enzyme reactor coupled online with nano liquid chromatography with mass spectrometry detection.
• Brandtzaeg, Ole Kristian; Wilson, Steven Ray Haakon; Lundanes, Elsa; Reubsaet, Leon; Krauss, Stefan & Martinsen, Ørjan Grøttem (2018). Online sample Preparation Platforms for Targeted Nano Liquid Chromatography Mass Spectrometry Bioanalysis. Series of dissertations submitted to the Faculty of Mathematics and Natural Sciences, University of Oslo.. 1968.
• Hassine, Esma Ben; Berg, Henriette Engen; Brandtzaeg, Ole Kristian; Vehus, Tore; Wilson, Steven Ray Haakon & Lundanes, Elsa (2018). Capillary poly(styrene-co-octadecene-co-divinylbenzene) monolithic columns.
• Hvinden, Ingvild Comfort; Rise, Frode; Berg, Henriette Engen; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). Approaches to untargeted metabolomics of glioblastoma: NMR and MS.
• Hvinden, Ingvild Comfort; Wilson, Steven Ray Haakon; Rise, Frode; Berg, Henriette Engen & Lundanes, Elsa (2018). Nuclear magnetic resonance spectroscopy based metabolomics discovers biomarkers of glioblastoma drug response. Show summary

  Glioblastoma is the most common and aggressive form of brain cancer. Even with comprehensive treatment regimes, patients face an expected survival of only 15 months. Currently, methods for assessing treatment response are lacking; it is difficult to accurately determine the efficacy of a treatment. The goal of the present study was to contribute to treatment assessment by scouting for metabolic biomarkers occurring in response to exposure to chemotherapeutic agents temozolomide (TMZ) and sepantronium bromide (YM155). Untargeted metabolomics of lysate from cultured glioblastoma cells was carried out with liquid state proton nuclear magnetic resonance (NMR) spectroscopy at resonance frequency 800 MHz. Spectral data were analyzed with two different multivariate statistical methods: principal component analysis (PCA) and partial least squares (PLS) regression. For YM155, two biomarker candidates were found: citric and lactic acid. Citric acid appeared to increase most in samples from cell lines less sensitive to YM155. Lactic acid decreased in all cell lines and was considered a more general biomarker of treatment exposure. TMZ-treated samples were not distinguishable from control samples, most likely due to too short exposure time (24 hours). Analyses with nano hydrophilic interaction liquid chromatography coupled with mass spectrometry (MS) corroborated the findings by NMR spectroscopy and statistical analyses. Both citric acid and lactic acid are biomarker candidates, but a more detailed understanding of their fluctuations in glioblastoma during treatment is needed. Nevertheless, they represent genuine candidates and should be considered for further in vivo magnetic resonance spectroscopy (MRS) studies. In the future, the biomarkers could be monitored with MRS, allowing a more unambiguous and personalized assessment of response to treatment in individual patients.

• Kular, Ramneet Kaur; Wilson, Steven Ray Haakon; Røberg-Larsen, Hanne & Lundanes, Elsa (2018). Towards ultrafast liquid chromatography of 3’-phosphoadenosine-5’-phosphosulfate using a short hydrophilic interaction chromatography column.
• Kular, Ramneet Kaur; Wilson, Steven Ray Haakon; Røberg-Larsen, Hanne & Lundanes, Elsa (2018). Towards ultrafast liquid chromatography of 3’-phosphoadenosine-5’-phosphosulfate using zwitterionic hydrophilic interaction liquid chromatography-mass spectrometry.
• Olsen, Christine; Brandtzaeg, Ole Kristian; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). On-line trapping of proteins with monolith-bound drugs, as a tool in drug target discovery.
• Pabst, Oliver; Martinsen, Ørjan Grøttem; Tronstad, Christian & Wilson, Steven Ray Haakon (2018). Electrical properties of human skin: From linear recordings of exogenous electrodermal activity to non-linear memristor measurements. Series of dissertations submitted to the Faculty of Mathematics and Natural Sciences, University of Oslo.. 1971.
• Racz, Beatrix; Wilson, Steven Ray Haakon; Rise, Frode & Lundanes, Elsa (2018). Identification of a major UV absorbing compound in goose aqueous humour. Show summary

  About 5% of solar radiation comes from the ultraviolet (UV) region. It carries high energy and can do serious damage to living organisms. The eye of animals and humans is a critically exposed area and contains UV absorbing compounds like proteins, tryptophan, tyrosine, ascorbic acid and uric acid in the aqueous humour (AH) for protection. In addition, an unknown component (compound X) which causes red-shift in the UV absorbance spectrum at 254 nm has been observed in heavily exposed species like goose, which migrates at 10 000 m altitude. X is a major absorber. UV measurements confirmed the presence of a compound in geese which has higher absorption at 254 nm compared to other species. Compound X also has fluorescence activity that resembled indole-functionality. Aqueous humour samples from different species (goose, chicken, turkey) were measured by nuclear magnetic resonance spectroscopy (NMR). The proton NMR spectra did not show any specific high abundant compound in the goose eye at the aromatic region compared to the other species, thus NMR could not reveal what made the difference between in UV absorbance. Mass spectrometry with atmospheric pressure photoionization (APPI) has earlier showed that the molar mass of the compound X could be 149 g/mole, which suggests that compound X might be the indole: 5,6-dihydroxyindole (DHI). DHI has a central role in the biosynthesis of melanin which takes place in melanocyte cells. DHI tends to easily be polymerized and one of the results is a notable change in its solubility. It was not possible to obtain a suitable external standard which did not undergo auto-polymerization, mirroring the observations with the isolated compound.To further test the hypothesis of DHI being compound X, reversed phase (RP) high performance liquid chromatography (HPLC) and hydrophilic interaction liquid chromatography (HILIC) were performed. Ascorbic acid co-eluted with the most abundant peak (compound X) in RP-HPLC. In HILIC the most abundant peak was not ascorbic acid. Hence, compound X is not ascorbic acid, and subsequent experiments showed that it was neither the UV absorbing compounds tryptophan, tyrosine or uric acid. After using several analytical tools, the hypothesis that compound X is 5,6-dihydroxyindole was strengthened. However an external standard is still necessary to provide accurate information.

• Racz, Beatrix; Zawadzka, Malgorzata Elzbieta; Skytte Af Sätra, Jenny Marie; Ringvold, Amund; Rise, Frode; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). Identification of “compound X” in goose aqueous humour.
• Røberg-Larsen, Hanne; Hutchinson, Sam Alex; Solheim, Stian; Wilson, Steven Ray Haakon; Lundanes, Elsa & Thorne, James L (2018). Oxysterols in breast cancer.
• Røberg-Larsen, Hanne; Sande, Maria Therese; Hutchinson, Samantha A; Solheim, Stian Kjønnås; Lundanes, Elsa; Thorne, James L & Wilson, Steven Ray Haakon (2018). Rapid determination of oxysterols in breast cancer tumors.
• Sandås, Elise Mørk; Elgstøen, Katja B. Prestø; Østeby, Anja; Skogvold, Hanne Bendiksen & Wilson, Steven Ray Haakon (2018). Liquid chromatography - Orbitrap mass spectrometry is a useful tool in untargeted metabolomics analysis of dried blood spots in clinical chemistry. Show summary

  There is a need for comprehensive analysis of the dried blood spot (DBS) metabolome to study both new and known inborn errors of metabolism (IEM). The purpose of this study was to complete and evaluate an untargeted metabolomics method using liquid chromatography – electrospray ionization – Q Exactive Orbitrap mass spectrometry (MS), for analysis of one punch (3.2 mm diameter corresponding to about 3 µL whole blood) of a DBS. The criteria for evaluation and measurements were inspired by stringency applied by the World Anti-Doping Agency. In this regard, the instrument repeatability and assay reproducibility were satisfactory with relative standard deviation (% RSD) of peak areas below 10 % and retention times below1 %, using a pooled control sample, injected three times each day during sample analysis for 11 days. Compared to perimeter punches, analyzing center punches improved the repeatability. An increased organic solvent amount in the reconstitution solution, and reduced m/z range of the MS (focusing more on each m/z increases sensitivity) increased the number of hydrophobic and low-abundant compounds detected, respectively. The quantification of most of the method evaluation compounds (acylcarnitines and amino acids) was satisfactory, with linear correlation of R2 above 0.99 in signal vs. concentration plots, using 1-4 punches (about 3, 6, 9 and 12 µL whole blood) of the DBS, but some polar compounds were affected by matrix effects. Tandem MS data was acquired to secure better identification. Combining the method with bioinformatics, one could identify changes between samples taken after free diet, overnight fasting (12 hours) and prolonged fasting (36 hours), using only 3 µL blood on paper. The method will be used to detect differences in the metabolome of patient and healthy samples in research and in future diagnostics.

• Skjærvø, Øystein; Pilarova, Veronika; Khalikova, Maria; Reubsaet, Leon; Halvorsen, Trine Grønhaug; Gjelstad, Astrid; Øiestad, Elisabeth Leere; Lundanes, Elsa; Wilson, Steven Ray Haakon & Pedersen-Bjergaard, Stig (2018). Those Who Can, Teach: Pharma Stars. The Analytical Scientist.  ISSN 2051-4077.  1118, s 40- 45
• Skjærvø, Øystein; Pilarova, Veronika; Khalikova, Maria; Reubsaet, Leon; Halvorsen, Trine Grønhaug; Gjelstad, Astrid; Øiestad, Elisabeth Leere; Lundanes, Elsa; Wilson, Steven Ray Haakon & Pedersen-Bjergaard, Stig (2018). Those Who Can, Teach: Pharma Stars. The Analytical Scientist.  ISSN 2051-4077.  1118, s 40- 45
• Skogvold, Hanne Bendiksen; Sandås, Elise Mørk; Østeby, Anja; Rootwelt, Helge; Arnesen, Camilla Elene; Wilson, Steven Ray Haakon; Rønning, Per Ola & Elgstøen, Katja B. Prestø (2018). Development and evaluation of an LC-Orbitrap MS method for untargeted metabolomics of dried blood spots.
• Skottvoll, Frøydis Sved; Nilsen, Ola; Krauss, Stefan; Sullivan, Gareth & Wilson, Steven Ray Haakon (2018). Integrating highly miniaturized LC-MS systems with “Organ on a Chip”; the future of preclinical modelling.
• Skytte Af Sätra, Jenny Marie; Nielsen, Claus Jørgen; Van Bavel, Albert; Lundanes, Elsa & Wilson, Steven Ray Haakon (2018). Developing procedures for trace analysis of small amines in water, using LC-MS and GC-MS.
• Solheim, Stian Kjønnås; Lundanes, Elsa; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2018). Exploring selectivity and sensitivity for oxysterol measurements using liquid chromatography-mass spectrometry (LC-MS).
• Vehus, Tore; Wilson, Steven Ray Haakon; Lundanes, Elsa; Krauss, Stefan; Halvorsen, Trine Grønhaug & Morth, Jens Preben (2018). Nano Liquid Chromatography-Tandem Mass Spectrometry Platforms for Determination of Low Abundant Wnt/Beta-Catenin Proteins in Cancer Research. Series of dissertations submitted to the Faculty of Mathematics and Natural Sciences, University of Oslo.. 1962.
• Arnesen, Camilla Elene; Wilson, Steven Ray Haakon; Elgstøen, Katja B. Prestø & Østeby, Anja (2017). Optimization of liquid chromatographic parameters for untargeted metabolomics of dried blood spots.
• Bjørseth, Kamilla; Wilson, Steven Ray Haakon; Brandtzaeg, Ole Kristian & Lundanes, Elsa (2017). Evaluation of exosome isolation techniques for hydrophilic interaction liquid chromatography-mass spectrometry based metabolomics.
• Demir, Deniz; Abele, Silvija; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon & Lundanes, Elsa (2017). Development of an ultrasensitive method for multiresidue screening of pharmaceutical products in environmental water samples.
• Demir, Deniz; Lundanes, Elsa; Wilson, Steven Ray Haakon; Abele, Silvija & Røberg-Larsen, Hanne (2017). Automatic capillary liquid chromatography tandem mass spectrometry method for pharmaceutical products in environmental water samples.
• Johnsen, Elin Follaug; Lundanes, Elsa; Wilson, Steven Ray Haakon & Greibrokk, Tyge (2017). High performance analytical Tools for small molecules in biosamples. Series of dissertations submitted to the Faculty of Mathematics and Natural Sciences, University of Oslo.. 1803.
• Røberg-Larsen, Hanne; Horne, Hildegunn; Holme, Ane Moe; Holm, Maia Blomhoff; Roland, Marie Cecilie; Michelsen, Trond; Wilson, Steven Ray Haakon & Henriksen, Tore (2017). Human in vivo studies of placenta physiology: oxysterols and metabolomic profiling.
• Røberg-Larsen, Hanne; Skottvoll, Frøydis Sved; Solheim, Stian; Bjørseth, Kamilla; Thorne, James L; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Oxysterols in cancer.
• Skogvold, Hanne Bendiksen; Elgstøen, Katja B. Prestø; Østeby, Anja & Wilson, Steven Ray Haakon (2017). Laboratory diagnostics: Maximizing sensitivity of a Q-Exactive Orbitrap mass spectrometer for untargeted metabolomics of dried blood spots.
• Skottvoll, Frøydis Sved; Berg, Henriette Engen; Lund, Kaja; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon & Lundanes, Elsa (2017). Critical evaluation of isolation and characterization techniques of breast cancer exosomes.
• Solheim, Stian; Røberg-Larsen, Hanne; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Optimizing selectivity and sensitivity for oxysterol measurements using liquid chromatography-mass spectrometry (LC-MS).
• Sved Skottvoll, Frøydis; Lund, Kaja; Røberg-Larsen, Hanne; Berg, Henriette Sjånes; Thiede, Bernd; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Diagnostic potential of exosomes: The purification issue.
• Wilson, Steven Ray Haakon (2017). Smedslunds hjertesukk : Jan Smedslund er for resignert på empiriens vegne. Tidsskrift for Norsk Psykologforening.  ISSN 0332-6470.  54(5), s 488- 489

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