Autoclaving

Required equipments

• Autoclave scotch
• 1L tap water

Autoclaving liquids:

• Borosilicate bottle

Other:

• Glass beaker
• Aluminum foil

General procedure

1. Prepare items that will be autoclaved:

   • Liquids: Fill borosilicate bottle with solution. Untighten cap slightly and put autoclave tape across lid, touching bottle on both ends.
   • Others: Fill beaker and cover top with aluminum foil. Fasten with autoclave tape.

2. Fill autoclaver with 1L water and put items inside.

3. ...

Autoclaved water

Must be done 4-5 hours before use of water (for cooling down)

1. Get 5L bottles of water from chemistry (check quality resistivity > 1 Ohms) or use the stock in room V420.
2. Fill 1L borosilicate bottle with water
3. Put cap on, untighten it slightly
4. Put autoclave scotch the lid and the bottle. One piece of scotch should cover both
5. Put in autoclave and follow general procedure on autoclave

Autoclaving pasteur pipettes

1. Stock in room V420 or in cupboard in room V431
2. Put them in a 1L beaker, tips at the bottom, beware not to break any.
3. Put some aluminum foil on top to close the beaker and fasten it into place with autoclave tape
4. Put it in the autoclave and follow the general procedure on autoclave

Equipment

• Autoclave scotch
• 1L tap water

For autoclaving liquids:

• Borosilicate bottle

Other:

• Glass beaker
• Aluminum foil