Cell seeding

This is Meng's protocol

1. On the day of cell seeding, remove the fibronectin solution, add 500ul 0.2% pluronics solution (inside and outside the stencil). Use tweezers to remove the stencil from the outer edge.
2. Replace the old pluronics with new 800 ul Pluronic, passivate for 10 min at 37 degrees.
3. Wash thrice with 1xPBS before cell seeding.
4. Number of cells: for RPE1, I seed 5x10^4 cells.
5. Remove unattached cells after 10 mins. Add fresh media.
6. Wait for at least 2 hr before imaging.