Morten Skage

• Cobb, Lauren Louise; de Muinck, Erik; Kollias, Spyros; Skage, Morten; Gilfillan, Gregor & Sydenham, Markus A. K. [Show all 8 contributors for this article] (2024). High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method. PLOS ONE. ISSN 1932-6203. 19(3). doi: 10.1371/journal.pone.0300865.
• Dussex, Nicolas; Tørresen, Ole Kristian; van der Valk, Tom; Le Moullec, Mathilde; Veiberg, Vebjørn & Tooming-Klunderud, Ave [Show all 17 contributors for this article] (2023). Adaptation to the High-Arctic island environment despite long-term reduced genetic variation in Svalbard reindeer. iScience. ISSN 2589-0042. 26(10). doi: 10.1016/j.isci.2023.107811. Full text in Research Archive Show summary

  Typically much smaller in number than their mainland counterparts, island populations are ideal systems to investigate genetic threats to small populations. The Svalbard reindeer (Rangifer tarandus platyrhynchus) is an endemic subspecies that colonized the Svalbard archipelago ca. 6,000–8,000 years ago and now shows numerous physiological and morphological adaptations to its arctic habitat. Here, we report a de-novo chromosome-level assembly for Svalbard reindeer and analyze 133 reindeer genomes spanning Svalbard and most of the species’ Holarctic range, to examine the genomic consequences of long-term isolation and small population size in this insular subspecies. Empirical data, demographic reconstructions, and forward simulations show that long-term isolation and high inbreeding levels may have facilitated the reduction of highly deleterious—and to a lesser extent, moderately deleterious—variation. Our study indicates that long-term reduced genetic diversity did not preclude local adaptation to the High Arctic, suggesting that even severely bottlenecked populations can retain evolutionary potential.

• Hoff, Siv Nam Khang; Baalsrud, Helle Tessand; Tooming-Klunderud, Ave; Skage, Morten; Richmond, Todd & Obernosterer, Gregor [Show all 10 contributors for this article] (2018). Long‐read sequence capture of the haemoglobin gene clusters across codfish species. Molecular Ecology Resources. ISSN 1755-098X. 19(1), p. 245–259. doi: 10.1111/1755-0998.12955.
• Tørresen, Ole Kristian; Star, Bastiaan; Jentoft, Sissel; Reinar, William Brynildsen; Grove, Harald & Miller, Jason R. [Show all 16 contributors for this article] (2017). An improved genome assembly uncovers prolific tandem repeats in Atlantic cod. BMC Genomics. ISSN 1471-2164. 18, p. 1–23. doi: 10.1186/s12864-016-3448-x. Full text in Research Archive
• Star, Bastiaan; Hansen, Marianne Helén Selander; Skage, Morten; Bradbury, Ian R.; Godiksen, Jane Amtoft & Kjesbu, Olav Sigurd [Show all 7 contributors for this article] (2016). Preferential amplification of repetitive DNA during whole genome sequencing library creation from historic samples. Science and Technology of Archaeological Research. ISSN 2054-8923. 2(1), p. 36–45. doi: 10.1080/20548923.2016.1160594. Full text in Research Archive
• Hatteland, Bjørn Arild; Solhøy, Torstein; Schander, Christoffer; Skage, Morten; Proschwitz, Ted von & Noble, Leslie R. (2015). Introgression and differentiation of the invasive slug Arion vulgaris from native A. ater. Malacologia. ISSN 0076-2997. 58(1-2), p. 303–321. doi: 10.4002/040.058.0210.
• Star, Bastiaan; Nederbragt, Alexander Johan; Hansen, Marianne Helén Selander; Skage, Morten; Gilfillan, Gregor Duncan & Bradbury, Ian R. [Show all 10 contributors for this article] (2014). Palindromic sequence artifacts generated during next generation sequencing library preparation from historic and ancient DNA. PLOS ONE. ISSN 1932-6203. 9(3). doi: 10.1371/journal.pone.0089676. Full text in Research Archive
• Star, Bastiaan; Nederbragt, Alexander Johan; Jentoft, Sissel; Grimholt, Unni; Malmstrøm, Martin & Gregers, Tone Fredsvik [Show all 42 contributors for this article] (2011). The genome sequence of Atlantic cod reveals a unique immune system. Nature. ISSN 0028-0836. 477(7363), p. 207–210. doi: 10.1038/nature10342. Full text in Research Archive
• Hatteland, Bjørn Arild; Symondson, William O. C.; King, R. A.; Skage, Morten; Schander, Christoffer & Solhøy, Torstein (2011). Molecular analysis of predation by carabid beetles (Carabidae) on the invasive Iberian slug Arion lusitanicus. Bulletin of entomological research. ISSN 0007-4853. 101(6), p. 675–686. doi: 10.1017/S0007485311000034.
• Lattman, H; Lättman, Håkan; Lindblom, Louise; Mattsson, Jan-Eric; Milberg, Per & Skage, Morten [Show all 7 contributors for this article] (2009). Estimating the dispersal capacity of the rare lichen Cliostomum corrugatum. Biological Conservation. ISSN 0006-3207. 142(8), p. 1870–1878. doi: 10.1016/j.biocon.2009.03.026.
• Petrusek, Adam; Hobæk, Anders; Nilssen, Jens Petter; Skage, Morten; Cerný, Martin & Brede, Nora [Show all 7 contributors for this article] (2008). A taxonomic reappraisal of the European Daphnia longispina complex (Crustacea, Cladocera, Anomopoda). Zoologica Scripta. ISSN 0300-3256. 37(5), p. 507–519. doi: 10.1111/j.1463-6409.2008.00336.x. Show summary

  Jens Petter Nilssen har angitt Niels Henrik Abel Centre, N-4980 Gjerstad, Norway i artikkelen/aa

• Skage, Morten & Schander, Christoffer (2007). DNA from formalin-fixed tissue: extraction or repair? That is the question. Marine Biology Research. ISSN 1745-1000. 3(5), p. 289–295. doi: 10.1080/17451000701473942. Show summary

  Establishing effective DNA-based protocols for use on archival material fixed in formaldehyde (formalin) is a particularly challenging task. Formalin fixation induces cross-linking with nucleic acids and proteins, thereby reducing the amount and quality of the extracted DNA. Previous attempts have primarily focused on optimizing DNA extraction protocols. Here we focus on the use of enzymes capable of in vitro repair of DNA extracts prior to amplification of the nucleic acids by the polymerase chain reaction (PCR). The amplification success of mitochondrial DNA was greater using the repair enzyme assay (56%) than with the regular PCR assay (20%), and even more convincing results were obtained with the amplified nuclear ribosomal region (91% versus 21%). These results indicate that in vitro repair of DNA damage (depurinated sites, strand nicks and base modifications) increases the number of samples that amplify, amplify to a greater extent and amplify fewer ancillary bands and that DNA repair has been overlooked as a way of improving the efficiency of molecular methods used on formalin-fixed samples. Fidelity has not been specifically investigated, but preliminary results indicate that misincorporation is not a major problem.

• Skage, Morten; Hobek, Anders; Ruthova, Štĕpánka; Keller, Barbara; Petrusek, Adam & Sed'a, Jaromír R [Show all 7 contributors for this article] (2007). Intra-specific rDNA-ITS restriction site variation and an improved protocol to distinguish species and hybrids in the Daphnia longispina complex. Hydrobiologia. ISSN 0018-8158. 594, p. 19–32. doi: 10.1007/s10750-007-9090-5.
• Nilssen, JP; Hobæk, Anders; Petrusek, Adam & Skage, Morten (2007). Restoring Daphnia lacustris GO Sars, 1862 (Crustacea, Anomopoda): A cryptic species in the Daphnia longispina group. Hydrobiologia. ISSN 0018-8158. 594(1), p. 5–17. doi: 10.1007/s10750-007-9076-3.
• Coyer, James A.; Hoarau, Galice; Skage, Morten; Stam, Wytze T. & Olsen, Jeanine L. (2006). Origin of Fucus serratus (Heterokontophyta; Fucaceae) populations in Iceland and the Faroes: a microsatellite-based assessment. European journal of phycology. ISSN 0967-0262. 41(2), p. 235–246. doi: 10.1080/09670260600652820.
• Skage, Morten; Gabrielsen, Tove M & Rueness, Jan (2005). A molecular approach to investigate the phylogenetic basis of three widely used species groups in the red algal genus Ceramium (Ceramiales, Rhodophyta). Phycologia. ISSN 0031-8884. 44(4), p. 353–360.
• Skage, Morten; Gabrielsen, Tove M. & Rueness, Jan (2005). A molecular approach to investigate the phylogenetic basis of three widely used species groups in the red algal genus Ceramium (Ceramiales, Rhodophyta). Phycologia. ISSN 0031-8884. 44(4), p. 353–360.
• Skage, Morten (2004). Daphnia Galeata × D. Longispina Hybrids in Western Norway. Hydrobiologia. ISSN 0018-8158. 526(1), p. 55–62.

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• Star, Bastiaan; Jentoft, Sissel; Matschiner, Michael; Nederbragt, Alexander Johan; Kjesbu, Olav Sigurd & Berg, Paul Ragnar [Show all 12 contributors for this article] (2015). Does historic DNA provide genomic evidence for recent selection in Atlantic cod?
• Tørresen, Ole Kristian; Jentoft, Sissel; Star, Bastiaan; Sandve, Geir Kjetil F.; Skage, Morten & Hansen, Marianne Helén Selander [Show all 9 contributors for this article] (2014). A new, high quality reference genome assembly for Atlantic cod.
• Tørresen, Ole Kristian; Walenz, Brian; Grove, Harald; Lien, Sigbjørn; Knight, James & Star, Bastiaan [Show all 15 contributors for this article] (2014). A new, high quality reference genome assembly for Atlantic cod.
• Matschiner, Michael; Star, Bastiaan; Berg, Paul Ragnar; Tørresen, Ole Kristian; Nederbragt, Alexander Johan & Hansen, Marianne Helén Selander [Show all 11 contributors for this article] (2014). Population differentiation and local adaptation in Atlantic cod.
• Nederbragt, Alexander Johan; Tørresen, Ole Kristian; Skage, Morten; Tooming-Klunderud, Ave ; Hansen, Marianne Helén Selander & Jentoft, Sissel [Show all 9 contributors for this article] (2012). The long jump: Long distance mate pairs and long PacBio reads for the Salmon Genome Project.
• Star, Bastiaan; Nederbragt, Alexander Johan; Jentoft, Sissel; Grimholt, Unni; Malmstrøm, Martin & Gregers, Tone Fredsvik [Show all 18 contributors for this article] (2011). The genome of Atlantic cod reveals a unique immune system.
• Skage, Morten; Mikkelsen, Nina Therese & Sjøtun, Inga Kjersti (2009). GENETIC SEPARATION OF DIFFERENT ENTITIES OF MASTOCARPUS STELLATUS (STACKHOUSE) GUIRY. Phycologia. ISSN 0031-8884. 48(4), p. 121–122.
• Grimholt, Unni; Nederbragt, Alexander Johan; Rounge, Trine Ballestad; Malmstrøm, Martin; Star, Bastiaan & Tooming-Klunderud, Ave [Show all 12 contributors for this article] (2009). The cod genome project.
• Grimholt, Unni; Malmstrøm, Martin; Nederbragt, Alexander Johan; Rounge, Trine Ballestad; Jentoft, Sissel & Tooming-Klunderud, Ave [Show all 10 contributors for this article] (2009). The lack of cod MHC class II.
• Tooming-Klunderud, Ave ; Nederbragt, Alexander Johan; Skage, Morten & Jakobsen, Kjetill Sigurd (2009). The Ultra-high Throughput Sequencing Platform at the University of Oslo.
• Hatteland, Bjørn Arild; Noble, Leslie R; Schander, Christoffer; Skage, Morten; Solhøy, Torstein & Symondson, W. O. C. (2007). Differentiation between the invasive Arion lusitanicus and the native Arion ater in western Norway combining morphology and genetics.
• Schander, Christoffer & Skage, Morten (2006). DNA from formalinized tissue - Extraction or PCR that's the question.
• Skage, Morten & Schander, Christoffer (2006). How to preserve genetic material – the formalin problem.
• Lättman, Håkan; Ekman, Stefan; Lindblom, Louise; Mattsson, Jan-Eric; Milberg, Per & Skage, Morten (2006). Regional occurrence of Cliostomum corrugatum is limited by habitat availability.
• Olseng, Christine; Aarø, Lars; Erga, Svein Rune; Samdal, IA; Skage, Morten & Naustvoll, L..J. [Show all 8 contributors for this article] (2005). Vertical migration, growth, genetics and toxicity among different strains of Norwegian Protoceratium reticulatum (Dinophyceae).
• Skage, Morten; Barth, Julia Maria Isis; Jentoft, Sissel; Øverli, Øyvind & Jakobsen, Kjetill Sigurd (2017). Genetisk bakgrunn hos ulv (Canis lupus) i Norge og Skandinavia. Universitetet i Oslo. Full text in Research Archive Show summary

  Formålet med denne undersøkelsen var å identifisere mulige krysninger mellom hund og ulv i dagens skandinaviske ulvebestand. I tillegg ønsket vi å finne ut i hvor stor grad det er mulig å gjenkjenne innblanding av hund i ulvens arvemateriale (hybridisering og tilbake-krysninger). Metodene som vi har anvendt i undersøkelsen er dyrere og mer tidkrevende enn det dagens forvaltningsmetodikk legger opp til (mikrosatelittanalyse), men gir bedre oppløsning over hele arvemassen og muliggjør deteksjon av svake hybridsignaler. Analysene er basert på data fra hel-genomsekvensering av ulv (dagens skandinaviske bestand og andre ulv), historisk ulv (Nordreisa 1962, Dyrøy 1963, Målselv 1963, og Stor Elvdal 1964), ulvehund (tsjekkisk ulvehund), primitive hunderaser (dingo, basenji, tibetansk mastiff) og moderne hunderaser (boxer etc), alle med ulik geografisk opprinnelse. De norske individene som er sekvensert i denne undersøkelsen kommer alle fra svenske føderevir, men har lagt ut på vandring og blitt avlivet i Norge (Stange, Nord-Odal, Sykkylven, Elverum og Trysil), og inngikk i det som betraktes som en felles skandinavisk bestand. Våre analyser viser: ● Den skandinaviske bestanden grupperer sammen med andre europeiske ulver, og ulver fra Asia, kalt «old world gruppen» i PCA (bilag1) ● Nylig hybridisering mellom ulv og hund ble ikke påvist. ● Genetiske analyser indikerer historisk innblanding av hund i alle analyserte ulver, også i skandinavisk bestand, men det er ikke mer innslag av hund i skandinavisk bestand enn hva som er funnet i andre populasjoner.

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