Spyridon Kollias

• Batista, Sonia; Ozorio, Rodrigo; Kollias, Spyridon; Dhanasiri, Anusha; Lokesh, Jep; Kiron, Viswanath; Valente, Luisa M.P. & Fernandes, Jorge (2016). Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants. Aquaculture.  ISSN 0044-8486.  458, s 149- 157 . doi: 10.1016/j.aquaculture.2016.03.002 Full text in Research Archive. Show summary

  Senegalese sole (Solea senegalensis) is a highly valued flatfish that grows well with diets containing plant ingredients but their effects on immune competence is still a matter of debate. The current study aimed to examine changes in innate immune parameters and gut microbiota in Senegalese sole fed with 35% or 72% of plant ingredients with or without probiotic or yeast supplementation. Overall, fish fed diets with 72% of plant ingredients showed lower transcript levels of key immune- and stress-related genes in distal intestine, rectum and head-kidney than the 35% diets. In particular, hsp90b mRNA levels in distal intestine were down-regulated by 70% and 60% with the use of high content of plant ingredients in the diet containing the multispecies probiotic and autolyzed yeast, respectively. Denaturing gradient gel electrophoresis showed lower similarity values for distal intestine than rectum. Also fish fed high content of plant ingredients displayed lower similarity values, pointing to a difference in the microbial populations between fish fed different plant ingredients content on the diet. Our data revealed that inclusion of plant ingredients was associated with differences in gene expression and a more diverse microbiota profile but without a significant effect on growth performance. Moreover, probiotic supplementation resulted in up-regulation of hsp90b, gpx, cat and apoa1 transcript levels in distal intestine concomitantly with a growth rate reduction compared to non-supplemented fish.

• Smolina, Irina; Kollias, Spyridon; Jüterbock, Alexander; Coyer, James A. & Hoarau, Galice Guillaume (2016). Variation in thermal stress response in two populations of the brown seaweed, Fucus distichus, from the Arctic and subarctic intertidal. Royal Society Open Science.  ISSN 2054-5703.  3 . doi: 10.1098/rsos.150429 Full text in Research Archive.
• Kollias, Spyros; Poortvliet, Marloes; Smolina, Irina & Hoarau, Galice (2015). Low cost sequencing of mitogenomes from museum samples using baits capture and Ion Torrent. Conservation Genetics Resources.  ISSN 1877-7252.  7(2), s 345- 348 . doi: 10.1007/s12686-015-0433-7 Full text in Research Archive.
• Poortvliet, Marloes; Olsen, Jeanine L.; Croll, Donald A; Bernardi, Giacomo; Newton, Kelly; Kollias, Spyros; O'Sullivan, John; Fernando, Daniel; Stevens, Guy; Magaña, Felipe Galván; Seret, Bernard; Wintner, Sabine & Hoarau, Galice (2015). A dated molecular phylogeny of manta and devil rays (Mobulidae) based on mitogenome and nuclear sequences. Molecular Phylogenetics and Evolution.  ISSN 1055-7903.  83, s 72- 85 . doi: 10.1016/j.ympev.2014.10.012
• Smolina, Irina; Kollias, Spyros; Møller, Eva Friis; Lindeque, Penelope K.; Sundaram, Arvind; Fernandes, Jorge & Hoarau, Galice (2015). Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis. Marine Ecology Progress Series.  ISSN 0171-8630.  534, s 79- 93 . doi: 10.3354/meps11398 Full text in Research Archive.
• Jüterbock, Alexander; Kollias, Spyros; Smolina, Irina; Fernandes, Jorge; Coyer, James A.; Olsen, Jeanine L & Hoarau, Galice (2014). Thermal stress resistance of the brown alga Fucus serratus along the North-Atlantic coast: Acclimatization potential to climate change. Marine Genomics.  ISSN 1874-7787.  13, s 27- 36 . doi: 10.1016/j.margen.2013.12.008 Full text in Research Archive. Show summary

  Seaweed-dominated communities are predicted to disappear south of 45° latitude on North-Atlantic rocky shores by 2200 because of climate change. The extent of predicted habitat loss, however, could be mitigated if the seaweeds' physiology is sufficiently plastic to rapidly acclimatize to the warmer temperatures. The main objectives of this study were to identify whether the thermal tolerance of the canopy-forming seaweed Fucus serratus is population-specific and where temperatures are likely to exceed its tolerance limits in the next 200 years. We measured the stress response of seaweed samples from four populations (Norway, Denmark, Brittany and Spain) to common-garden heat stress (20 °C–36 °C) in both photosynthetic performance and transcriptomic upregulation of heat shock protein genes. The two stress indicators did not correlate and likely measured different cellular components of the stress response, but both indicators revealed population-specific differences, suggesting ecotypic differentiation. Our results confirmed that thermal extremes will regularly reach physiologically stressful levels in Brittany (France) and further south by the end of the 22nd century. Although heat stress resilience in photosynthetic performance was higher at the species' southern distributional edge in Spain, the hsp expression pattern suggested that this edge-population experienced reduced fitness and limited responsiveness to further stressors. Thus, F. serratus may be unable to mitigate its predicted northward shift and may be at high risk to lose its center of genetic diversity and adaptability in Brittany (France). As it is an important intertidal key species, the disappearance of this seaweed will likely trigger major ecological changes in the entire associated ecosystem.

• Smolina, Irina; Kollias, Spyros; Poortvliet, Marloes; Nielsen, Torkel Gissel; Lindeque, Penelope; Castellani, Claudia; Møller, Eva Friis; Blanco-Bercial, Leocadio & Hoarau, Galice (2014). Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification. Molecular Ecology Resources.  ISSN 1755-098X.  14(5), s 1072- 1079 . doi: 10.1111/1755-0998.12241 Full text in Research Archive.

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• Smolina, Irina; Kollias, Spyros; Møller, Eva Friis; Lindeque, Penelope & Hoarau, Galice (2014). Transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis.
• Lazado, Carlo Cabacang; Nagasawa, Kazue; Kollias, Spyros; Babiak, Igor & Fernandes, Jorge (2013). Photic sensitivity and circadian rhythmicity of the fast muscle transcriptome in Atlantic cod, Gadus morhua.
• Lazado, Carlo Cabacang; Nagasawa, Kazue; Kumaratunga, Hiruni; Babiak, Igor; Kollias, Spyros & Fernandes, Jorge (2013). Exploring the transcriptional landscape of the circadian rhythm in the fast skeletal muscle of Atlantic cod using next-generation sequencing technologies.
• Smolina, Irina; Kollias, Spyros; Nielsen, Torkel Gissel; Lindeque, Penelope & Hoarau, Galice (2013). Genome- and transcriptome-assisted development of InDel markers for solving identification challenge of calanoid copepode Calanus finmarchicus and C. glacialis.

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