The Mass Spectrometry Common Laboratory is a collaborative initiative that brings together the range of mass spectrometry (MS)-based research expertise available across different departments at UiO.
Mass Spectrometry Laboratory and Core Facility
Department of Chemistry, Faculty of Mathematics and Natural Sciences
Leader: Professor Einar Uggerud, Head of the Department of Chemistry
The Mass Spectometry Laboratory operates as a core facilty for users at the Department of Chemistry and the Department of Pharmacy, as well as external users.
We specialise in the analysis of small and medium-sized molecules (pure samples or in mixture) using electrospray ionization, chemical ionization, gas chromatography (GC) and high performance liquid chromatography (HPLC).
Cancer Proteomics Laboratory and Core Facility
Department of Biosciences, Faculty of Mathematics and Natural Sciences
Visit Cancer Proteomics Laboratory and Core Facility webpage
Leader: Dr. Bernd Thiede, Head of the Proteomics Core Facility at the Department of Biosciences
The Cancer Proteomics Laboratory performs quantitative and qualitative protein and proteome analysis for a broad user group, particularly at UiO. The facility forms one node of the National Network of Advanced Proteomics Infrastructure.
Services include consultancy, various aspects of sample handling and preparation for MS analysis, protein identification with MALDI/MS and LC/MS analysis, as well as assistance with downstream data analysis. The facility specialises in the analysis of post-translational modifications (PTMs). We also have experience in ‘top down’ proteomics (i.e. analysis of intact proteins rather than peptides).
Proteomics Core Facility
Department of Immunology, Faculty of Medicine and Oslo University Hospital
The Proteomics Core Facility offers mass spectrometry-based proteomics and biological mass spectrometry services to a broad user group, primarily aimed at academic users associated with the South-Eastern Norway Regional Health Authority (Helse Sør-Øst). The facility forms one node of the National Network of Advanced Proteomics Infrastructure, where facility leader Dr Tuula Nyman is Project Manager.
We have extensive experience in protein identification and quantification, and in analysis of post-translational modifications and interactions within and between proteins. We have a particular focus on proteomics related to immunology, as well as applications in clinical proteomics. The facility recently acquired a timsTOF fleX mass spectrometer from Bruker Daltonics, which enables MALDI-based imaging mass spectrometry (determination of specific molecular masses in 2D-space, e.g. on tissue samples).
Department of Pharmacy and Department of Chemistry, Faculty of Mathematics and Natural Sciences
The Bioanalytics Environment consists of two partners; the Smart Protein Analysis Group and the Bioanalytical Chemistry Group.
Contact: Professor Léon Reubsaet, Leader of the Smart Protein Analysis Group, Department of Pharmacy
This professional environment involves a long-standing (since 2007) collaboration between two research groups at MatNat: Bioanalytical Chemistry (Chemistry) and Smart Protein Analysis (Pharmacy). Both groups work towards the development of advanced analysis methodology related to mass spectrometry, including miniaturized analysis methods and innovative sample preparation methods with extremely high sensitivity, e.g. for use in patient monitoring, doping and substance abuse, as well as medical diagnostics.
We offer expertise and advice in method and strategy development within the determination of biologically active compounds in biological matrices.
Both research groups include partners of the National Network of Advanced Proteomics Infrastructure.
The Metabolomics Lab
Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine
The laboratory can carry out analyses on biological fluids, tissue biopsies as well as matrices from a variety of different vegetables. In addition to equipment and expertise, the laboratory has great flexibility that allows the development and establishment of new methods and analyses in a short time for current and future needs.
The department works broadly in the field of nutrition, with particular emphasis on biological mechanisms for connections between disease and diet.
Structural Mass Spectrometry Lab
Centre for Molecular Medicine Norway (NCMM), Faculty of Medicine
The laboratory applies hydrogen-deuterium exchange coupled to MS (HDX-MS) to gain insights into structures and dynamics of proteins and protein complexes. The technique can be used to identify binding sites in high affinity protein complexes where Kd is in low nM range (for example protein-drug complexes or antigen-antibody complexes). The technique can be used to identify allosteric changes in proteins induced by ligand binding or introduction of post-translational modifications.
We have a state-of-art Waters HDX Synapt G2S system with ETD and ion-mobility and LEAP PAL robot for sample preparation.
The projects are assessed for feasibility, and due to extensive optimization necessary for each project, they are usually considered as collaborations. Non-academic collaborations are possible and involve higher fees.
If you would like to perform MS analyses as part of your research project, please contact the relevant laboratory leader using the details provided below each laboratory.
If you are unsure which partner is best suited to your project, please contact the laboratory coordinator Bernd Thiede.
About Mass Spectrometry (MS)
Mass spectrometry (MS) is a central technology to many scientific disciplines, particularly in the life sciences; from physical chemistry and organic chemistry/drug chemistry, via metabolomics and proteomics, to medical imaging and diagnostics. At the University of Oslo (UiO) and Oslo University Hospital (OUS), the mass spectrometric competence and associated instrument fleet is widely distributed in many environments, including several departments within the Faculty of Medicine and the Faculty of Mathematics and Natural Sciences.
The MS Common Laboratory brings together all groups with a strong focus and expertise in MS-based research. Read more about the individual partners, also known as nodes, that make up the MS common lab, including information on the MS instruments, research expertise, and services available within each node.