Moving EME into protein analysis…

In biochemical research, proteins are often labelled with a fluorescent compound for visualisation. However, prior to visualisation, excess of label has to be removed to reduce the background fluorescence. In a recent work published in Analytical Chemistry, we tested the idea of using electromembrane extraction (EME) for such purposes. The philosophy behind this work is to push EME in new directions, and to inspire other scientists to explore and develop new applications of EME. Some of these future applications will hopefully be “killer applications”, and EME will be an established technique in the analytical toolbox…  

 

Read the paper…

Electromembrane Extraction of Unconjugated Fluorescein Isothiocyanate from Solutions of Labeled Proteins Prior to Flow Induced Dispersion Analysis

Restan, M.S, Pedersen, M.E., Jensen, H., Pedersen-Bjergaard, S.

Analytical Chemistry 91 (2019) 6702-6708

Published Mar. 11, 2020 2:38 PM - Last modified Mar. 11, 2020 2:38 PM