Taking the temperature on sample preparation of polar substances

In collaboration with scientists from Hangzhou Normal University, Central South University, and other international partners, we have reviewed the rapidly evolving landscape of sample preparation for polar metabolites in biological samples. From amino acids and nucleotides to sugar phosphates and CoA derivatives, we examine the strengths, limitations, and emerging trends of modern extraction technologies, including SPE, SPME, LPME, and electromembrane extraction (EME). The review provides practical guidance for selecting appropriate methodologies while highlighting opportunities for greener, more automated, and higher-performance workflows in metabolomics and bioanalysis...

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