Trial lecture - time and place
04.06.2024, 10.15, Aud. 2, Helga Eng
Advantages and challenges with different matrices in forensic applications
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Conferral summary
Dette arbeidet utforsket grunnleggende aspekter av mikroekstraksjonsprinsippene væskefase-mikroekstraksjon (LPME) og elektromembranekstraksjon (EME). Denne kunnskapen ble implementert i utviklingen av metoder for rettsmedisinske anvendelser for prøver av fullblod og hår. Bruken av miniatyrisert prøveforberedelse er et skritt mot grønnere analytiske praksiser.
Main research findings
Traditional sample preparation methods in forensic analysis typically require significant volumes of potentially hazardous organic solvents. Growing concerns about environmental repercussions put emphasis on adopting greener practices. In recent years, liquid-phase microextraction (LPME) and electromembrane extraction (EME) have emerged as promising greener alternatives to conventional approaches. LPME and EME operate on the principle of transferring analytes across a small volume of organic membrane from and aqueous sample to an aqueous acceptor phase. In EME, an electric field is applied across the organic liquid membrane, facilitating transfer by electrokinetic migration.
The aim of this study was to explore fundamental aspects relating to the selection of extraction principle (LPME or EME) and experimental conditions such as extraction format, and apply this knowledge to investigate the use of LPME and EME for a variety of forensic applications.
Paper I and Paper IV found that extraction performance of both techniques is confined by the analyte log P value. Analytes that are too water soluble or too fat soluble, analytes are not extracted efficiently. Furthermore, it was found that EME was more efficient than LPME. Paper II, III, and V built on the knowledge from the fundamental papers and involved method development of forensic applications for new psychoactive substances from whole blood and common drugs of abuse from hair using both LPME and EME.
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