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• Hanson, Ingunn; Pitman, Kathinka Elinor & Edin, Nina Frederike Jeppesen (2023). The Role of TGF-β3 in Radiation Response. International Journal of Molecular Sciences. ISSN 1661-6596. 24(8). doi: 10.3390/ijms24087614. Full text in Research Archive Show summary

  Transforming growth factor-beta 3 (TGF-β3) is a ubiquitously expressed multifunctional cytokine involved in a range of physiological and pathological conditions, including embryogenesis, cell cycle regulation, immunoregulation, and fibrogenesis. The cytotoxic effects of ionizing radiation are employed in cancer radiotherapy, but its actions also influence cellular signaling pathways, including that of TGF-β3. Furthermore, the cell cycle regulating and anti-fibrotic effects of TGF-β3 have identified it as a potential mitigator of radiation- and chemotherapy-induced toxicity in healthy tissue. This review discusses the radiobiology of TGF-β3, its induction in tissue by ionizing radiation, and its potential radioprotective and anti-fibrotic effects.

• Hanson, Ingunn; Pitman, Kathinka Elinor; Altanerova, Ursula; Altaner, Čestmír; Malinen, Eirik & Edin, Nina Frederike Jeppesen (2022). Low-Dose-Rate Radiation-Induced Secretion of TGF-β3 Together with an Activator in Small Extracellular Vesicles Modifies Low-Dose Hyper-Radiosensitivity through ALK1 Binding. International Journal of Molecular Sciences. ISSN 1661-6596. 23(15). doi: 10.3390/ijms23158147. Full text in Research Archive
• Pitman, Kathinka; Alluri, Santosh Reddy; Kristian, Alexandr; Aarnes, Eva-Katrine; Lyng, Heidi & Riss, Patrick [Show all 7 contributors for this article] (2019). Influx rate of 18F-fluoroaminosuberic acid reflects cystine/glutamate antiporter expression in tumour xenografts. European Journal of Nuclear Medicine and Molecular Imaging. ISSN 1619-7070. 46(10), p. 2190–2198. doi: 10.1007/s00259-019-04375-8. Full text in Research Archive
• Alluri, Santosh Reddy; Pitman, Kathinka; Malinen, Eirik & Riss, Patrick (2019). Synthesis, radiosynthesis, and positron emission tomography neuroimaging using 5-[18 F]fluoro-L-amino suberate. Journal of labelled compounds & radiopharmaceuticals. ISSN 0362-4803. 63(1), p. 6–14. doi: 10.1002/jlcr.3814. Full text in Research Archive Show summary

  System xc‐ (Sxc‐) has emerged as a new biological target for PET studies to detect oxidative and excitotoxic stress. Notably, applications have, thus far, been limited to tumour imaging although Sxc‐) may play a major role in neurodegeneration. The synthesis procedures of tosylate precursor and its translation to Sxc‐ PET tracer 5[18F]fluoro‐L‐amino suberate by manual and automated radiosyntheses are described. A brain‐PET study has been conducted to evaluate the tracer uptake into brain in healthy mice.

• Pitman, Kathinka; Bakke, Kine Mari; Kristian, Alexandr & Malinen, Eirik (2019). Ultra-early changes in vascular parameters from dynamic contrast enhanced MRI of breast cancer xenografts following systemic therapy with doxorubicin and liver X receptor agonist. Cancer Imaging. ISSN 1740-5025. 19:88, p. 1–7. doi: 10.1186/s40644-019-0280-y. Full text in Research Archive Show summary

  Background Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) may be used to depict tumour vascular structure and for therapy response assessment in various tumour sites. The purpose of the current work is to examine whether ultra-early changes in tumour physiology following cytotoxic treatment with doxorubicin and liver X receptor (LXR) agonist GW3965 are detectable by DCE-MRI. Methods 36 female, athymic nude foxn1nu mice with bilaterally implanted breast cancer xenografts (17 with ER-positive HBCx34, 19 with triple-negative HBCx39) were randomised in the following treatment groups; control, GW3965 (40 mg/kg p.o.), doxorubicin (8 mg/kg i.v.) and a combination therapy of GW3965 and doxorubicin. DCE-MRI (3D FLASH on a 7 T preclinical scanner) was performed at baseline and one and six days after onset of treatment. Wash-in (30 s p.i.) and wash-out (300 s p.i.) enhancement were quantified from dynamic uptake curves, before voxel-by-voxel fitting to the pharmacokinetic Tofts model and generation of maps for the resulting parameters Ktrans, νe and νB. Treatment effect was evaluated by univariate repeated measures mixed-effects maximum likelihood regression models applied to median tumour data. Results We found no effects of any treatment 24 h post treatment. After 6 days, doxorubicin given as both mono- and combination therapy gave significant increases of ~ 30% in wash-in enhancement (p < 0.011) and Ktrans (p < 0.017), and 40–50% in νB (p < 0.024) for HBCx34, but not for HBCx39. No effects of GW3965 were observed at any time (p > 0.1). Conclusions Twenty-four h after onset of treatment was too early to evaluate treatment effects by DCE-MRI. Early enhancement and Ktrans were approximately equally sensitive metrics to capture treatment effects six days pt. Pharmacokinetic modelling however allowed us to attribute the observed effect to changes in tumour perfusion rather than increased retention.

• Pitman, Kathinka; Rusten, Espen; Kristian, Alexandr & Malinen, Eirik (2015). Variability of dynamic 18F-FDG-PET data in breast cancer xenografts. Acta Oncologica. ISSN 0284-186X. 54(9), p. 1399–1407. doi: 10.3109/0284186X.2015.1062140. Full text in Research Archive

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• Hanson, Ingunn; Pitman, Kathinka Elinor & Edin, Nina Frederike Jeppesen (2024). Mechanisms of TGF- β3 induced modulation of low dose hyperradiosensitivity. Full text in Research Archive
• Pitman, Kathinka Elinor; Hanson, Ingunn & Edin, Nina Frederike Jeppesen (2024). Mechanisms of TGFb3 induced modulation of low dose hyper-radiosensitivity. Full text in Research Archive
• Hanson, Ingunn; Pitman, Kathinka Elinor; Thiede, Bernd; Cai, Yiqing & Edin, Nina Frederike Jeppesen (2021). Proteomic Analysis of Low Dose-Rate Primed Cells and Extracellular Exosomes. Full text in Research Archive
• Pitman, Kathinka Elinor; Bakke, Kine Mari; Kristian, Alexandr & Malinen, Eirik (2019). Ultra-early changes in vascular parameters from dynamic contrast enhanced MRI of breast cancer xenografts following systemic therapy. Full text in Research Archive
• Pitman, Kathinka; Bakke, Kine Mari; Kristian, Alexandr & Malinen, Eirik (2017). Ultra-early changes in vascular DCE-MRI parameters following treatment of breast cancer xenografts with liver X receptor agonist and doxorubicin. Full text in Research Archive
• Pitman, Kathinka Elinor; Rusten, Espen; Kristian, Alexandr & Malinen, Eirik (2015). Variability of Dynamic 18FDG-PET Data in Breast Cancer Xenografts. Full text in Research Archive
• Pitman, Kathinka; Rusten, Espen; Kristian, Alexandr & Malinen, Eirik (2015). Inter- and intra-variability of dynamic FDG-PET data in breast cancer xenografts. Radiotherapy and Oncology. ISSN 0167-8140. 115, p. s642–s642. doi: 10.1016/S0167-8140(15)41175-2. Full text in Research Archive
• Pitman, Kathinka; Rusten, Espen; Kristian, Alexandr & Malinen, Eirik (2014). Reproducibility of dynamic 18F‐FDG‐PET data in breast cancer xenografts. Full text in Research Archive
• Pitman, Kathinka (2019). Dynamic PET and DCE-MRI with Pharmacokinetic Modelling for Cancer Characterisation and Response Monitoring. Preclinical Studies in Tumour Xenograft Models. Det matematisk-naturvitenskapelige fakultet. Full text in Research Archive

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