New publication: Mapping enzyme activity on tissue by functional‐mass spectrometry imaging

By Brett R. Hamilton, David L. Marshall, Nicholas R. Casewell, Robert A. Harrison, Stephen J. Blanksby, and Eivind A. B. Undheim in Angewandte Chemie International Edition. Open Access.

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Abstract

Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High‐resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high‐resolution maps of phospholipase activity and specificity, which could subsequently be compared to histological images of the same section. Functional MSI thus represents a new and generalisable method for imaging biological activity in situ.


Angew. Chem. Int. Ed.
Volume 59, Issue10
March 2, 2020
Pages 3855-3858
First published: 18 December 2019
DOI: 10.1002/anie.201911390
Publication webpage.


Brett R. Hamilton, David L. Marshall, Nicholas R. Casewell, Robert A. Harrison, Stephen J. Blanksby, and Eivind A. B. Undheim*

* Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biosciences, University of Oslo, Oslo, Norway. See the publication webpage for full author information.

Tags: Angew. Chem. Int. Ed.;
Published Apr. 16, 2020 3:32 PM - Last modified Apr. 16, 2020 4:11 PM