Andrea Angelov Eltvik

• Carvalho, Mariana Mazetto de; Ellefsen, Christiane Færestrand; Eltvik, Andrea Angelov; Hiorth, Marianne & Samuelsen, Anne Berit C. (2025). Chemical structure characterization of polysaccharides using diffusion ordered NMR spectroscopy (DOSY). Carbohydrate Polymers. ISSN 0144-8617. 349(Part B). doi: 10.1016/j.carbpol.2024.123021. Full text in Research Archive
• Mohamed, Warsan Nora; Butt, Hussain Shakeel; Schmidt, Thomas J.; Eltvik, Andrea Angelov; Wu, Desheng & Malterud, Karl Egil [Show all 9 contributors for this article] (2025). Biflavonoids and bi- and tricoumarins from Daphne mezereum and inhibition of TNF-α secretion. Phytochemistry. ISSN 0031-9422. 229. doi: 10.1016/j.phytochem.2024.114308. Full text in Research Archive Show summary

  Daphne mezereum L. (Thymelaeaceae) was an important medicinal plant in Norway during the 18th and 19th centuries and used against diseases such as diarrhea, swelling, stomach pain, and tuberculosis. Five previously undescribed phenolic compounds, including two biflavonoids with a catechin core structure, two tricoumarins, and one bicoumarin, together with ten known compounds were isolated from a 50% EtOH extract of the bark of D. mezereum. Using NMR, HRESIMS, acid hydrolysis, and circular dichroism spectra, the biflavonoids were identified as 3′-hydroxygenkwanol A and 3′-hydroxydihydrodaphnodorin B, and the coumarins were identified as 3‴-O-acetyltriumbellin, triumbellin 4‴-O-β-d-glucopyranoside, and daphnogitin-7-O-β-d-glucopyranoside. The absolute configuration of dihydrodaphnodorin B was for the first time established as 2R, 3S, 2″S, 3″S. Daphnin, syringin, 3′-hydroxydihydrodaphnodorin B, dihydrodaphnodorin B, and neochamaejasmin A and B were identified as the major secondary metabolites in the extract. Neochamaejasmin A and B showed the most potent inhibition of TNF-α secretion in Con A stimulated peripheral blood mononuclear cells (PBMCs) with 71.3 ± 3.4 and 83.5 ± 11.5% inhibition, respectively, at 50 μM.

• Eltvik, Andrea Angelov; Inngjerdingen, Marit & Wangensteen, Helle (2025). In vitro anti-inflammatory properties of twelve Norwegian medicinal plants. Journal of Ethnopharmacology. ISSN 0378-8741. 352. doi: 10.1016/j.jep.2025.120159. Full text in Research Archive Show summary

  Ethnopharmacological relevance Today, inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease represent a major health burden in Western societies. Such diseases are treated with drugs aiming at suppressing inflammatory symptoms. In Norway, several medicinal plants share a history of ethnomedical use towards the treatment of various inflammatory conditions which could represent sources of new anti-inflammatory drugs. Aim of the study This study focused on exploring the potential of understudied traditional medicinal plants used in Norway to treat inflammation of different origin. Through phytochemical and biological screening methods, we aimed to identify plants with anti-inflammatory properties that also showed a high proportion of phenolic compounds. Based on these results, such plants would be of interest and relevance for further work including isolation and characterization of phytochemicals that could serve as novel anti-inflammatory drugs. Materials and methods A systematic literature survey of historical records from the 1800s and onwards was performed searching for plants used to treat various inflammatory diseases. Twelve plants were selected and subjected to accelerated solvent extraction to obtain extracts rich in phenolic compounds, namely dichloromethane and 80 % ethanol extracts. These were studied in bioassays assessing their in vitro anti-inflammatory effects. Their ability to inhibit nitric oxide (NO) and TNF-α cytokine production was measured, and their protection against indomethacin induced damage of the intestinal epithelial barrier was evaluated using differentiated Caco-2 cells. The total phenolic content of the extracts was determined, and 1H NMR spectroscopy and HPLC-DAD was performed for phytochemical profiling. Results Several of the plants presented moderate to high bioactivity, namely Matricaria discoidea, Alnus incana, Geranium sylvaticum, Antennaria dioica, Tanacetum vulgare and Malva moschata. A stronger effect was observed among the dichloromethane extracts in NO and TNF-α inhibition, while the 80 % ethanol extracts showed greater protection of the barrier integrity. M. discoidea, A. incana and G. sylvaticum are of particular interest, presenting consistently high bioactivity. Their 1H NMR spectra also showed a high proportion of phenolic compounds. Conclusion Both A. incana and M. discoidea are scarcely studied regarding their bioactivity and chemical composition, being potential candidates for the discovery of novel anti-inflammatory drugs. Based on the results obtained from this study, further work will focus on these two plants, including isolation of phenolic compounds and assessing their biological activities.

• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2024). Coating of liposomes with β-glucans from the king oyster mushroom (Pleurotus eryngii). Colloids and Surfaces A: Physicochemical and Engineering Aspects. ISSN 0927-7757. 700. doi: 10.1016/j.colsurfa.2024.134783. Full text in Research Archive

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• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2024). β-glucans from the king oyster mushroom (Pleurotus eryngii) as a novel source material for coating liposomes. Full text in Research Archive
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Ellefsen, Christiane Færestrand; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Liposome surface coating using distinct β-glucans fractions from Pleurotus eryngii. Full text in Research Archive
• Carvalho, Mariana Mazetto de; Eltvik, Andrea Angelov; Hiorth, Marianne & Samuelsen, Anne Berit C. (2023). Development of β-glucans coated liposomes for targeting to the cells of the immune system. Full text in Research Archive

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