yijp

• Røyne, Anja; Phua, Yi Jing; Le, Simone Balzer; Eikjeland, Ina Grosås; Josefsen, Kjell Domaas & Markussen, Sidsel [Vis alle 10 forfattere av denne artikkelen] (2019). Towards a low CO2 emission building material employing bacterial metabolism (1/2): The bacterial system and prototype production. PLOS ONE. 14(4). doi: 10.1371/journal.pone.0212990. Fulltekst i vitenarkiv Vis sammendrag

  The production of concrete for construction purposes is a major source of anthropogenic CO2 emissions. One promising avenue towards a more sustainable construction industry is to make use of naturally occurring mineral-microbe interactions, such as microbial-induced carbonate precipitation (MICP), to produce solid materials. In this paper, we present a new process where calcium carbonate in the form of powdered limestone is transformed to a binder material (termed BioZEment) through microbial dissolution and recrystallization. For the dissolution step, a suitable bacterial strain, closely related to Bacillus pumilus, was isolated from soil near a limestone quarry. We show that this strain produces organic acids from glucose, inducing the dissolution of calcium carbonate in an aqueous slurry of powdered limestone. In the second step, the dissolved limestone solution is used as the calcium source for MICP in sand packed syringe moulds. The amounts of acid produced and calcium carbonate dissolved are shown to depend on the amount of available oxygen as well as the degree of mixing. Precipitation is induced through the pH increase caused by the hydrolysis of urea, mediated by the enzyme urease, which is produced in situ by the bacterium Sporosarcina pasteurii DSM33. The degree of successful consolidation of sand by BioZEment was found to depend on both the amount of urea and the amount of glucose available in the dissolution reaction.

• Phua, Yi Jing; Pegoretti, A & Ishak, ZA Mohd (2018). Experimental analysis and theoretical modeling of the mechanical behavior of starch-grafted-polypropylene/kenaf fibers composites. Polymer Composites. ISSN 0272-8397. 39(9), s. 3289–3299. doi: 10.1002/pc.24344. Fulltekst i vitenarkiv
• Phua, Yi Jing & Røyne, Anja (2018). Bio-cementation through controlled dissolution and recrystallization of calcium carbonate. Construction and Building Materials. ISSN 0950-0618. 167, s. 657–668. doi: 10.1016/j.conbuildmat.2018.02.059. Fulltekst i vitenarkiv Vis sammendrag

  We present an approach to bio-cementation of sand where the calcium source is prepared by dissolving powdered limestone (chalk) in lactic acid. Cementation is achieved through enzyme induced carbonate precipitation (EICP) with Jack Bean urease. The real-time nucleation and growth of crystals, crystal morphology and mechanical strength of consolidated samples was studied for dissolved chalk solution as well as calcium chloride (CaCl2) and calcium lactate solutions. Solutions containing lactate were found to yield spherical calcite crystals. The compressive strengths of consolidated samples ranged from 0.06 to 2.8 MPa, increasing with the amount of precipitated CaCO3.

• Phua, Yi Jing; Pegoretti, Alessandro; Araujo, T. Medeiros & Ishak, Z. A. Mohd (2015). Mechanical and thermal properties of poly(butylene succinate)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biodegradable blends. Journal of Applied Polymer Science. ISSN 0021-8995. 132(47). doi: 10.1002/app.42815. Fulltekst i vitenarkiv

Se alle arbeider i NVA

• Røyne, Anja; Phua, Yi Jing; Eikjeland, Ina Grosås; Wentzel, Alexander; Le, Simone Balzer & Josefsen, Kjell Domaas [Vis alle 14 forfattere av denne artikkelen] (2018). BioZEment 2.0: Towards a low CO2 emission building material employing bacterial metabolism. Fulltekst i vitenarkiv
• Røyne, Anja; Phua, Yi Jing; Borch, Anita; Throne-Holst, Harald; Josefsen, Kjell Domaas & Le, Simone Balzer [Vis alle 11 forfattere av denne artikkelen] (2017). Kunsten å arbeide tverrfaglig. 4. Fulltekst i vitenarkiv
• Røyne, Anja; Phua, Yi Jing; Eikjeland, Ina Grosås; Le, Simone Balzer; Markussen, Sidsel & Josefsen, Kjell Domaas [Vis alle 7 forfattere av denne artikkelen] (2017). Concrete production through microbially induced dissolution and re-precipitation of limestone. Fulltekst i vitenarkiv
• Phua, Yi Jing & Røyne, Anja (2016). Morphology and Polymorphism of Calcium Carbonate Precipitated from Different Calcium Sources via Enzyme Induced Carbonate Precipitation. Fulltekst i vitenarkiv

Se alle arbeider i NVA