Disputation:Frøydis Sved Skottvoll

MSc. Frøydis Sved Skottvoll at the Department of Chemistry, Faculty of Mathematics and Natural Sciences, is defending her thesis «Liver organoids, mass spectrometry and separation science» for the degree of Philosophiae Doctor.

Bildet av kandidaten.


Due to the ongoing Pandemic, the Disputation will be held digital using Zoom. The Chair of Defence will lead the Disputation and the Defence technician will solve technical issues.

Ex auditorio questions: The Chair of Defence will invite the audience to ex auditorio questions. These can be asked orally, by clicking "Participants - Raise hand" in the Zoom menu. The Zoom-host will grant you to speak in the meeting. 

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Order the Dissertation as PDF from this email address. Please add name of the Candidate: a.c.gartner@kjemi.uio.no

Trial lecture

11th. of March 2022 at 10:15 AM, Zoom

Title: "Towards understanding biological pathways through proteomics: the role of affinity chromatography techniques”

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Conferral summary

Organoider er en ny teknologi som kan endre fremtidens medisin, ved å etterlikne menneskets egne organer i miniatyr. I dette doktorgradsarbeidet er avanserte, sensitive analysemetoder tatt i bruk for å analysere lever-organoiders virkemåte, ved å måle små molekyler og makromolekyler, som legemidler og proteiner.

Main research findings

Lab-grown liver organoids display human organ functionality

Organoids are predicted to change the future of medicine by acting as an in vitro 3D representation of human organ functionality. Organoids are grown from patient derived stem cells or adult tissue, which promise to enable new and faster drug discovery, disease modeling and personalized medicine under more realistic conditions. Organoids are still at the developmental stage and fundamental questions concerning the functionality of the developed organoids remain to be answered. Compared to other biosampling techniques, mass spectrometry (MS) has rarely been used in organoid analyses. This thesis leverages MS and separation science to analyze the biological properties of liver organoids, spanning from protein identification to small molecule drug metabolite detection. With the aim of developing selective, high throughput analyses and online biosampling integration, both conventional and cutting edge electromembrane extraction (EME) sample preparation approaches were explored.

Using bioanalytical tools including MS and separation science, we could establish that the liver organoids displayed liver organ functionality. The bioanalytical strategies we developed for liver organoid analyses contribute to greater insight into organoid response and functionality and could be important tools in future organoid development. Furthermore, online organoid integration using electromembrane extraction developed here could be used as a starting point towards developing future organ-on-a-chip systems integrated with mass spectrometry, which would be valuable in drug development, disease modeling, and personalized medicine.

Published Mar. 1, 2022 11:19 AM - Last modified Mar. 1, 2022 11:39 AM