The Disputation will be live streamed for everyone else.
The livestream will be activated 15 minutes before the Defense starts.
Trial lecture
February 28th, 10:00 AM, Auditorium 3, Chemistry building
Trial lecture title: "Animal research and the "3Rs": Analytical opportunities and challenges (3Rs = Replacements, reduction, and refinement)"
Kreeringssammendrag/Conferral summary
Arbeidet omhandler utviklingen av avanserte metoder for analyse av legemidler i innovative stamcelle-deriverte modeller som etterligner menneskelige organer – såkalte organoider og organ-on-chip-systemer. Ved hjelp av automatiserte analyseteknikker kan legemiddelnedbrytning og toksisitet studeres mer effektivt og uten behov for dyreforsøk. Arbeidet legger til rette for sikrere, mer etisk og presis legemiddelutvikling.
Main research findings
Testing drugs efficiently without animal models is a key challenge in drug development. This work focuses on "organoids" – miniature, lab-grown replicas of human organs – and organ-on-chip systems, which mimic organ functions. These innovative models offer exciting potential for personalized medicine and drug discovery but are challenging to analyze due to their small size and complex composition.
To address these challenges, advanced methods with minimal manual preparation were developed to study how drugs and their metabolites behave in these systems. First, a novel "organ-in-a-column" system was created, connecting liver organoids to a mass spectrometer for real-time, automated tracking of drug metabolism. Next, a validated self-cleaning analytical tool was developed to process over 1,000 consecutive organoid samples without manual interference, eliminating extra preparation steps. Finally, a cutting-edge technique called electromembrane extraction was tested to remove impurities, ensuring samples are ready for simpler detectors and high-speed analysis.
These breakthroughs expand the toolkit and make it faster, easier, and more reliable to study drugs in organoid models and organ-on-chip. By refining these non-animal methods, this work lays the foundation for safer, more ethical drug development tailored to human biology.
Candidate contact information
LinkedIn: https://www.linkedin.com/in/stiankogler/
E-post: stian.kogler@kjemi.uio.no
Phone: +47 930 377 37
Adjudication committee
Professor Sebastiaan Eeltink
Chemical Engineering and Separation Science, Vrije Universiteit Brussel, Belgium
Research leader Anna Nordborg
Bioteknologi og nanomedisin, SINTEF Industri, Norway
Professor Anja Olafsen Sjåstad
Department of Chemistry, University of Oslo, Norway
Supervisors
Professor Hanne Røberg-Larsen
Department of Chemistry, University of Oslo, Norway
Professor Steven Ray Haakon Wilson
Department of Chemistry, University of Oslo, Norway
Stefan Johannes Karl Krauss
Institute for Basic Medical Sciences, University of Oslo, Norway
Professor Ola Nilsen
Department of Chemistry, University of Oslo, Norway
Chair of defence
Professor Ute Krengel
Department of Chemistry, University of Oslo, Norway