Ragnhild Elisabeth Paulsen

Image of Ragnhild Elisabeth Paulsen
Norwegian version of this page
Phone +47-22844936
Fax +47-22844944
Room 253 ZEB-bygningen
Username
Visiting address Sem Sælands vei 3 Farmasibygningen 0371 OSLO
Postal address Postboks 1068 Blindern 0316 OSLO
Other affiliations School of Pharmacy

Academic interests

Our research interests are molecular mechanisms of cell death and cell survival in the central nervous system. We use neuronal cell cultures as experimental models. Many of our studies involve glutamate, which is a neurotransmitter in the CNS that in high extracellular concentrations causes cell death e.g. following a stroke; or drugs such as glucocorticoids. Important findings are that certain steriods protect against glutamate-induced cell death; glutamate-induced cell death is caused by ROS, tranlocation of NGFI-B to the mitochondria, and caspase-3 activation.

Teaching

Higher education and employment history

Education:

  • Dr.philos, University of Oslo, 1989
  • Cand.pharm., University of Oslo, 1985

Employment history:

  • Professor, School of Pharmacy, University of Oslo, since 1996
  • Associate Professor, School of Pharmacy, University of Oslo 1995-1996
  • Sabbatical, Washington University School of Medicine, St. Louis, 1990-1992
  • Sabbatical, FIDIA-Georgetown Institute for the Neurosciences, Washington DC, 1989-1990
  • Scientist, Norwegian Defense Research Establishment, 1987-1995
  • PhD student (Norwegian Research Council), 1985-1987

Boards

  • Secretary, Norwegian Neuroscience Society
  • Member, Department board, School of Pharmacy, University of Oslo, 2013-2016

 

Tags: Pharmacology, Neurobiology, Neurotoxicology, Molecular neurobiology, Glutamate toxicity, Transcription factors

Publications

  • Sørvik, Irene Beate & Paulsen, Ragnhild Elisabeth (2017). High and low concentration of 17?-estradiol protect cerebellar granule neurons in different time windows. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  490(3), s 676- 681 . doi: 10.1016/j.bbrc.2017.06.100
  • Austdal, Lars Peter Engeset; Bjørnstad, Sigrid; Mathisen, Gro Haarklou; Aden, Petra; Mikkola, Ingvild; Paulsen, Ragnhild Elisabeth & Rakkestad, Kirsten Eline (2016). Glucocorticoid effects on cerebellar development in a chicken embryo model: exploring changes in PAX6 and Metalloproteinase-9 after exposure to dexamethasone. Journal of neuroendocrinology (Print).  ISSN 0953-8194.  28(12) . doi: 10.1111/jne.12438
  • Austdal, Lars Peter Engeset; Mathisen, Gro Haarklou; Løberg, Else Marit & Paulsen, Ragnhild Elisabeth (2016). Calcium-induced apoptosis of developing cerebellar granule neurons depends causally on NGFI-B. International Journal of Developmental Neuroscience.  ISSN 0736-5748.  55, s 82- 90 . doi: 10.1016/j.ijdevneu.2016.10.003
  • Bjørnstad, Sigrid; Samara, Athina; Erichsen, Aage; Paulsen, Ragnhild Elisabeth; Glover, Joel & Roald, Borghild (2016). Hampered lung maturation in methimazole-induced hypothyroidism in fetal chicken: morphological and molecular correlates to human fetal development. Neonatology.  ISSN 1661-7800.  110(2), s 83- 92 . doi: 10.1159/000444656
  • Bjørnstad, Sigrid; Austdal, Lars Peter Engeset; Roald, Borghild; Glover, Joel & Paulsen, Ragnhild Elisabeth (2015). Cracking the egg: potential of the developing chicken as a model system for nonclinical safety studies of pharmaceuticals. Journal of Pharmacology and Experimental Therapeutics.  ISSN 0022-3565.  355, s 386- 396 . doi: 10.1124/jpet.115.227025
  • Yazdani, Mazyar; Paulsen, Ragnhild Elisabeth; Gjøen, Tor & Hylland, Ketil (2015). Reactive Oxygen Species and Cytotoxicity in Rainbow Trout Hepatocytes: Effects of Medium and Incubation Time. Bulletin of Environmental Contamination and Toxicology.  ISSN 0007-4861.  94, s 193- 198 . doi: 10.1007/s00128-014-1433-0
  • Bjørnstad, Sigrid; Paulsen, Ragnhild Elisabeth; Erichsen, Aage; Glover, Joel & Roald, Borghild (2014). Type I and II pneumocyte differentiation in the developing fetal chicken lung: Conservation of pivotal proteins from birds to human in the struggle for life at birth. Neonatology.  ISSN 1661-7800.  105(2), s 112- 120 . doi: 10.1159/000355346
  • Rakkestad, Kirsten Eline; Sørvik, Irene Beate; Øverby, Gunn Rebecca; Debernard, Karen A. Boldingh; Mathisen, Gro Haarklou & Paulsen, Ragnhild Elisabeth (2014). 17a-estradiol down-regulates glutathione synthesis in serum deprived PC-12 cells. Free radical research.  ISSN 1071-5762.  48(10), s 1170- 1178 . doi: 10.3109/10715762.2014.930455
  • Solum, Eirik Johansson; Cheng, Jing-Jy; Sørvik, Irene Beate; Paulsen, Ragnhild Elisabeth; Vik, Anders & Hansen, Trond Vidar (2014). Synthesis and biological evaluations of new analogs of 2-methoxyestradiol: Inhibitors of tubulin and angiogenesis. European Journal of Medicinal Chemistry.  ISSN 0223-5234.  85, s 391- 398 . doi: 10.1016/j.ejmech.2014.08.002
  • Aziz, Gulzeb; Odlo, Kristin; Hansen, Trond Vidar; Paulsen, Ragnhild Elisabeth & Mathisen, Gro Haarklou (2013). Combretastatin A-4 and structurally related triazole analogues induce caspase-3 and reactive oxygen species-dependent cell death in PC12 cells. European Journal of Pharmacology.  ISSN 0014-2999.  703(1-3), s 25- 32
  • Mathisen, Gro Haarklou; Yazdani, Mazyar; Rakkestad, Kirsten Eline; Aden, Petra; Bodin, Johanna; Samuelsen, Mari; Nygaard, Unni Cecilie; Goverud, Ingeborg Løstegaard; Gaarder, Mona; Løberg, Else Marit; Bølling, Anette Kocbach; Becher, Rune & Paulsen, Ragnhild Elisabeth (2013). Prenatal exposure to bisphenol A interferes with the development of cerebellar granule neurons in mice and chicken. International Journal of Developmental Neuroscience.  ISSN 0736-5748.  31(8), s 762- 769 . doi: 10.1016/j.ijdevneu.2013.09.009
  • Åstrand, Ove Alexander Høgmoen; Austdal, Lars Peter Engeset; Paulsen, Ragnhild Elisabeth; Hansen, Trond Vidar & Rongved, Pål (2013). Synthesis and characterization of new selective Zn2+ fluorescent probes for functionalization: in vitro Cell imaging applications. Tetrahedron.  ISSN 0040-4020.  49(40), s 8645- 8654 . doi: 10.1016/j.tet.2013.07.049
  • Åstrand, Ove Alexander; Aziz, Gul zeb; Ali, Sidra Farzand; Paulsen, Ragnhild Elisabeth; Hansen, Trond Vidar & Rongved, Pål (2013). Synthesis and initial in vitro biological evaluation of two new zinc-chelating compounds: Comparison with TPEN and PAC-1. Bioorganic & Medicinal Chemistry.  ISSN 0968-0896.  21(17), s 5175- 5181 . doi: 10.1016/j.bmc.2013.06.037
  • Bølling, Anette Kocbach; Øvrevik, Johan; Samuelsen, Jan Tore; Holme, Jørn Andreas; Rakkestad, Kirsten Eline; Mathisen, Gro Haarklou; Paulsen, Ragnhild Elisabeth; Korsnes, Monica Suarez & Becher, Rune (2012). Mono-2-ethylhexylphthalate (MEHP) induces TNF-alpha release and macrophage differentiation through different signalling pathways in RAW264.7 cells. Toxicology Letters.  ISSN 0378-4274.  209(1), s 43- 50 . doi: 10.1016/j.toxlet.2011.11.016
  • Debernard, Karen A. Boldingh; Mathisen, Gro Haarklou & Paulsen, Ragnhild Elisabeth (2012). Differences in NGFI-B, Nurr1, and NOR-1 expression and nucleocytoplasmic translocation in glutamate-treated neurons. Neurochemistry International.  ISSN 0197-0186.  61(1), s 79- 88 . doi: 10.1016/j.neuint.2012.04.002
  • Strøm, Bjørn Oddvar & Paulsen, Ragnhild Elisabeth (2012). Apoptosis inducer NGFI-B is degraded by the proteasome and stabilized by treatment with EGF. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  417(4), s 1292- 1297 . doi: 10.1016/j.bbrc.2011.12.132
  • Aden, Petra; Paulsen, Ragnhild Elisabeth; Mæhlen, Jan; Løberg, Else Marit; Goverud, Ingeborg Løstegaard; Liestøl, Knut & Lømo, Jon (2011). Glucocorticoids dexamethasone and hydrocortisone inhibit proliferation and accelerate maturation of chicken cerebellar granule neurons. Brain Research.  ISSN 0006-8993.  1418, s 32- 41 . doi: 10.1016/j.brainres.2011.08.053
  • Debernard, Karen A. Boldingh; Aziz, Gul zeb; Gjesvik, Annine Thomassen & Paulsen, Ragnhild Elisabeth (2011). Cell death induced by novel procaspase-3 activators can be reduced by growth factors. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  413(2), s 364- 369 . doi: 10.1016/j.bbrc.2011.08.107
  • Mathisen, Gro Haarklou; Fallgren, Åsa Birgitta; Strøm, Bjørn Oddvar; Debernard, Karen A. Boldingh; Mohebi, Beata Urbanczyk & Paulsen, Ragnhild Elisabeth (2011). Delayed translocation of NGFI-B/RXR in glutamate stimulated neurons allows late protection by 9-cis retinoic acid. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  414(1), s 90- 95 . doi: 10.1016/j.bbrc.2011.09.028
  • Aziz, Gulzeb; Akselsen, Øyvind Wilhelm; Hansen, Trond Vidar & Paulsen, Ragnhild Elisabeth (2010). Procaspase-activating compound 1 induces a caspase-3-dependent cell death in cerebellar granule neurons. Toxicology and Applied Pharmacology.  ISSN 0041-008X.  247, s 238- 242
  • Rakkestad, Kirsten Eline; Holme, Jørn Andreas; Paulsen, Ragnhild E; Schwarze, Per E & Becher, Rune (2010). Mono(2-ethylhexyl) phthalate induces both pro- and anti-inflammatory responses in rat alveolar macrophages through crosstalk between p38, the lipoxygenase pathway and PPAR alpha. Inhalation Toxicology.  ISSN 0895-8378.  22(2), s 140- 150 . doi: 10.3109/08958370903019885
  • Strøm, Bjørn Oddvar; Aden, Petra; Mathisen, Gro Haarklou; Lømo, Jon; Davanger, Svend & Paulsen, Ragnhild Elisabeth (2010). Transfection of chicken cerebellar granule neurons used to study glucocorticoid receptor regulation by nuclear receptor 4A (NR4A). Journal of Neuroscience Methods.  ISSN 0165-0270.  193(1), s 39- 46 . doi: 10.1016/j.jneumeth.2010.08.012
  • Aden, Petra; Goverud, Ingeborg Løstegaard; Liestøl, Knut; Løberg, Else Marit; Paulsen, Ragnhild Elisabeth; Maehlen, Jan & Lømo, Jon (2008). Low-potency glucocorticoid hydrocortisone has similar neurotoxic effects as high-potency glucocorticoid dexamethasone on neurons in the immature chicken cerebellum. Brain Research.  ISSN 0006-8993.  1236, s 39- 48 . doi: 10.1016/j.brainres.2008.07.095
  • Fallgren, Åsa Birgitta; Mathisen, Gro Anita; Mæhlen, Jan; Blomhoff, Rune & Paulsen, Ragnhild Elisabeth (2007). Preconditioning with estradiol abolishes its neuroprotection in cerebellar neurons. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  352, s 966- 972 . doi: 10.1016/j.bbrc.2006.11.139
  • Maiga, Ababacar Ibrahim; Malterud, Karl Egil; Mathisen, Gro Haarklou; Paulsen, Ragnhild Elisabeth; Thomas-Oates, Jane; Bergström, Ed; Reubsaet, Jan Leo; Diallo, Drissa & Paulsen, Berit Smestad (2007). Cell protective antioxidants from the root bark of Lannea velutina A. Rich., a Malian medicinal plant. Journal of Medicinal Plants Research.  ISSN 1996-0875.  1(4), s 66- 79
  • Mathisen, Gro Anita; Thorkildsen, Inger Helene & Paulsen, Ragnhild Elisabeth (2007). Secretory PLA(2)-IIA and ROS generation in peripheral mitochondria are critical for neuronal death. Brain Research.  ISSN 0006-8993.  1153, s 43- 51 . doi: 10.1016/j.brainres.2007.03.067 Show summary
  • Hankø, Erik; Hansen, Thor Willy; Almaas, Runar; Paulsen, Ragnhild Elisabeth & Rootwelt, Terje (2006). Synergistic Protection of a General Caspase Inhibitor and MK-801 in Bilirubin-Induced Cell Death in Human NT2-N Neurons. Pediatric Research.  ISSN 0031-3998.  59(1), s 72- 77 . doi: 10.1203/01.pdr.0000191135.6358 Show summary
  • Jacobs, Chris; Aden, Petra Käte; Mathisen, Gro Anita; Khuong, Erica; Gaarder, Mona; Løberg, Else Marit; Lømo, Jon; Mæhlen, Jan & Paulsen, Ragnhild Elisabeth (2006). Chicken cerebellar granule neurons rapidly develop excitotoxicity in culture. Journal of Neuroscience Methods.  ISSN 0165-0270.  156, s 129- 135 Show summary
  • Jacobs, Chris; Trinh, Minh D.; Rootwelt, Terje; Lømo, Jon & Paulsen, Ragnhild Elisabeth (2006). Dexamethasone induces cell death which may be blocked by NMDA receptor antagonists but is insensitive to Mg2+ in cerebellar granule neurons. Brain Research.  ISSN 0006-8993.  1070, s 116- 123 . doi: 10.1016/j.brainres.2005.10.093 Show summary
  • Jacobs, Chris & Paulsen, Ragnhild Elisabeth (2005). Crosstalk between ERK2 and RXR regulates nuclear import of transcription factor NGFI-B. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  336, s 646- 652 . doi: 10.1016/j.bbrc.2005.08.143
  • Rosseland, Carola; Wierød, Lene; Oksvold, M.P.; Werner, H; Østvold, Anne C.; Thoresen, G. Hege; Paulsen, Ragnhild Elisabeth; Huitfeldt, Henrik & Skarpen, Ellen (2005). Cytoplasmic retention of peroxide-activated ERK provides survival in primary cultures of rat hepatocytes. Hepatology.  ISSN 0270-9139.  42, s 200- 2007
  • Jacobs, Chris; Boldingh, Karen Astrid; Slagsvold, Hege Holte; Thoresen, Gunn Hege & Paulsen, Ragnhild Elisabeth (2004). ERK2 prohibits apoptosis-induced subcellular translocation of orphan nuclear receptor NGFI-B/TR3. Journal of Biological Chemistry.  ISSN 0021-9258.  279(48), s 50097- 50101
  • Slagsvold, Hege H.; Rosseland, Carola; Jacobs, Chris; Khuong, Erica; Gaarder, Mona; Fallgren, Åsa Birgitta; Huitfeldt, Henrik & Paulsen, Ragnhild Elisabeth (2003). High molecular weight DNA fragments are processed by caspase sensitive or caspase independent pathways in cultures of cerebellar granule neurons. Brain Research.  ISSN 0006-8993.  984, s 111- 121
  • Slagsvold, Hege H.; Østvold, Anne Carine; Fallgren, Åsa Birgitta & Paulsen, Ragnhild Elisabeth (2002). Nuclear receptor and apoptosis initiator NGFI-B is a substrate for kinase ERK2. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  291, s 1146- 1150
  • Klungland, Arne; Paulsen, Ragnhild Elisabeth; Rolseth, Veslemøy; Yamada, Y.; Ueno, Y.; Wiik, Pål; Matsuda, A.; Seeberg, Erling Christen & Bjelland, Svein (2001). 5-Formyluracil and its nucleoside derivatives confer toxicity and mutagenicity to mammalian cells by interfering with normal RNA and DNA metabolism. Toxicology Letters.  ISSN 0378-4274.  119, s 71- 78
  • Oksvold, Morten Pedersen; Skarpen, Ellen; Wierød, Lene; Paulsen, Ragnhild Elisabeth & Huitfeldt, Henrik (2001). Re-localization of activated EGF receptor and its signal transducers to multivesicular compartments downstream of early endosomes in response to EGF. European Journal of Cell Biology.  ISSN 0171-9335.  80(4), s 285- 294
  • Paulsen, Ragnhild Elisabeth & Ottersen, Ole Petter (2000). Glutamat, apoptose og nekrose i nervesystemet. Tidsskrift for Den norske legeforening.  ISSN 0029-2001.  120, s 63- 64
  • Paulsen, Ragnhild Elisabeth & Ottersen, Ole Petter (2000). Glutamate, apoptose og nekrose i sentralnervesystemet. Tidsskrift for Den norske legeforening.  ISSN 0029-2001.  120, s 63- 64
  • Slagsvold, Hege H.; Marvik, Ole-Jørgen; Eidem, Guro; Kristoffersen, Nina & Paulsen, Ragnhild Elisabeth (2000). Detection of high molecular weight DNA fragments characteristic of early stage apoptosis in cerebellar granule cells exposed to glutamate. Experimental Brain Research.  ISSN 0014-4819.  135, s 173- 178
  • Olsen, Christel; Rustad, Anne; Fonnum, Frode; Paulsen, Ragnhild Elisabeth & Hassel, Bjørnar (1999). 3-nitropropionic acid: an astrocyte-sparing neurotoxin in vitro. Brain Research.  ISSN 0006-8993.  850, s 144- 149
  • Ciani, Elisabetta; Rizzi, Simona; Paulsen, Ragnhild Elisabeth & Contestabile, Antonio (1997). Chronic pre-explant blockade of the NMDA receptor affects survival of cerebellar granule cells explanted in vitro. Developmental Brain Research.  ISSN 0165-3806.  99, s 112- 117
  • Myhrer, Trond & Paulsen, Ragnhild Elisabeth (1997). Infusion of D-cycloserine into temporal-hippocampal areas and restoration of mnemonic function in rats with disrupted glutamatergic temporal systems. European Journal of Pharmacology.  ISSN 0014-2999.  328, s 1- 7
  • Paulsen, Ragnhild Elisabeth; Bardal, Kjersti Granås; Johnsen, Helge; Rolseth, Veslemøy & Sterri, Sigrun Hanne (1995). Three related brain nuclear receptors, NGFI-B, Nurr1, and NOR-1, as transcriptional activators. Journal of Molecular Neuroscience.  ISSN 0895-8696.  6, s 249- 255
  • Hadera, Mussie Ghezu; Andersen, Jannike Mørch & Paulsen, Ragnhild Elisabeth (2017). Effects of methadone in the developing chicken embryo as a model of neurodevelopmental toxicity. Reproductive Toxicology.  ISSN 0890-6238.  72, s 216- 217 . doi: https://doi.org/10.1016/j.reprotox.2017.06.030
  • Yazdani, Mazyar; Paulsen, Ragnhild Elisabeth; Gjøen, Tor & Hylland, Ketil (2013). Reactive oxygen species and cytotoxicity in rainbow trout hepatocytes: effects of medium and incubation time.
  • Yazdani, Mazyar; Paulsen, Ragnhild Elisabeth; Gjøen, Tor & Hylland, Ketil (2011). Comparison of three fluorescence probes for ROS detection in primary cultures of rainbow trout hepatocytes.
  • Yazdani, Mazyar; Ellesat, Kathrin; Paulsen, Ragnhild Elisabeth & Hylland, Ketil (2010). Developing a fish hepatocyte model for oxidative stress studies.
  • Debernard, Karen A. Boldingh; Strøm, Bjørn Oddvar; Jacobs, Chris & Paulsen, Ragnhild Elisabeth (2007). EGF Protects Neurons From Glutamate-Induced Cell Death by Erk2-Mediated Nuclear Targeting of Apoptosis-Inducer NGFI-B/TR3.
  • Debernard, Karen A. Boldingh; Strøm, Bjørn Oddvar; Jacobs, Chris & Paulsen, Ragnhild Elisabeth (2007). EGF protects neurons from cell death by ERK2-mediated nuclear targeting of apoptosis-inducer NGFI-B/TR3.
  • Debernard, Karen A. Boldingh; Strøm, Bjørn Oddvar; Jacobs, Chris & Paulsen, Ragnhild Elisabeth (2007). Vekstfaktoren EGF beskytter nerveceller mot død ved Erk2-mediert kjernelokalisering av apoptose-inducer NGFI-B.
  • Mathisen, Gro Anita; Fallgren, Åsa Birgitta; Gaarder, Mona; Mæhlen, Jan; Blomhoff, Rune & Paulsen, Ragnhild Elisabeth (2007). Preconditioning with estradiol abolishes its neuroprotection in cerebellar granule neurons.
  • Moen, Lars Fredrik; Movig, Trude; Jacobs, Chris; Debernard, Karen Boldingh; Mathisen, Gro Anita & Paulsen, Ragnhild Elisabeth (2007). Nuclear import and transcriptional activity of NGFI-B - effects of ATP, RXRa, RARa, 9cRA, ATRA, and TTNPB.
  • Debernard, Karen Boldingh; Jacobs, Chris; Thoresen, G. Hege & Paulsen, Ragnhild Elisabeth (2006). EGF protects cerebellar granule neurons from glutamate-induced cell death by ERK2-mediated inhibition of mitochondrial targeting of orphan nuclear receptor NGFI-B/TR3.
  • Aden, Petra; Jacobs, Chris; Minh, D. Trinh; Rootwelt, Terje; Lømo, Jon & Paulsen, Ragnhild Elisabeth (2006). Dexamethasone induces apoptotic cell death through NMDA receptor activation in cultured cerebellar granule cells.
  • Debernard, Karen Boldingh; Jacobs, Chris M & Paulsen, Ragnhild Elisabeth (2006). Activation of ERK2 leads to nuclear targeting of apoptosis inducer NGFI-B in cerebellar granule neurons.
  • Mathisen, Gro Anita & Paulsen, Ragnhild Elisabeth (2006). Different reactive oxygen species are critical for toxicity in two cell death models in cerebellar granule neurons.
  • Mathisen, Gro Anita & Paulsen, Ragnhild Elisabeth (2006). Different reactive oxygen species are critical for toxicity in two cell death models in cerebellar granule neurons. Free radical research.  ISSN 1071-5762.  40, s S91- S91
  • Aden, Petra; Jacobs, Chris; Khuong, Erica; Goverud, Ingeborg Løstegaard; Løberg, Else Marit; Paulsen, Ragnhild Elisabeth; Maehlen, Jan & Lømo, Jon (2004). Neurotoxicological studies in fetal chicken: Effect of glucocorticoids on apoptosis in cerebellar granule cells in vivo and in vitro.
  • Lindgren, Carola; Holte, Hege Ruge; Solberg, Kristin & Paulsen, Ragnhild Elisabeth (2000). Caspase 3, enzyme activity and level of expression, in rat cerebellar granule cells after glutamate exposure. Show summary
Published Nov. 17, 2010 3:26 PM - Last modified Dec. 11, 2017 2:20 PM