Winnie Eskild

• Antewi, Milton Boaheng; Dumitriu, Gianina Aurica; Simon, Jaione Santamaria; Sanchez Romano, Javier; Li, Ruomei & Smedsrød, Bård [Show all 9 contributors for this article] (2023). Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp gt/gt mouse model of slowly progressing liver fibrosis. PLOS ONE. ISSN 1932-6203. 18(11), p. 1–29. doi: 10.1371/journal.pone.0293526. Full text in Research Archive Show summary

  Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells with a unique, high endocytic clearance capacity for blood-borne waste macromolecules and colloids. This LSEC scavenger function has been insufficiently characterized in liver disease. The Glmpgt/gt mouse lacks expression of a subunit of the MFSD1/GLMP lysosomal membrane protein transporter complex, is born normal, but soon develops chronic, mild hepatocyte injury, leading to slowly progressing periportal liver fibrosis, and splenomegaly. This study examined how LSEC scavenger function and morphology are affected in the Glmpgt/gt model. FITC-labelled formaldehyde-treated serum albumin (FITC-FSA), a model ligand for LSEC scavenger receptors was administered intravenously into Glmpgt/gt mice, aged 4 months (peak of liver inflammation), 9–10 month, and age-matched Glmpwt/wt mice. Organs were harvested for light and electron microscopy, quantitative image analysis of ligand uptake, collagen accumulation, LSEC ultrastructure, and endocytosis receptor expression (also examined by qPCR and western blot). In both age groups, the Glmpgt/gt mice showed multifocal liver injury and fibrosis. The uptake of FITC-FSA in LSECs was significantly reduced in Glmpgt/gt compared to wild-type mice. Expression of LSEC receptors stabilin-1 (Stab1), and mannose receptor (Mcr1) was almost similar in liver of Glmpgt/gt mice and age-matched controls. At the same time, immunostaining revealed differences in the stabilin-1 expression pattern in sinusoids and accumulation of stabilin-1-positive macrophages in Glmpgt/gt liver. FcγRIIb (Fcgr2b), which mediates LSEC endocytosis of soluble immune complexes was widely and significantly downregulated in Glmpgt/gt liver. Despite increased collagen in space of Disse, LSECs of Glmpgt/gt mice showed well-preserved fenestrae organized in sieve plates but the frequency of holes >400 nm in diameter was increased, especially in areas with hepatocyte damage. In both genotypes, FITC-FSA also distributed to endothelial cells of spleen and bone marrow sinusoids, suggesting that these locations may function as possible compensatory sites of clearance of blood-borne scavenger receptor ligands in liver fibrosis.

• Blagotinšek Cokan, Kaja; Urlep, Žiga; Moškon, Miha; Mraz, Miha; Kong, Xiang Yi & Eskild, Winnie [Show all 9 contributors for this article] (2021). Common Transcriptional Program of Liver Fibrosis in Mouse Genetic Models and Humans. International Journal of Molecular Sciences. ISSN 1661-6596. 22(2), p. 1–21. doi: 10.3390/ijms22020832. Full text in Research Archive
• Lopez, David Massa; Thelen, Melanie; Stahl, Felix; Thiel, Christian; Linhorst, Arne & Sylvester, Marc [Show all 11 contributors for this article] (2019). The lysosomal transporter mfsd1 is essential for liver homeostasis and critically depends on its accessory subunit glmp. eLIFE. ISSN 2050-084X. 8, p. 1–27. doi: 10.7554/eLife.50025. Full text in Research Archive
• Mousavi, Seyed Ali; Skjeldal, Frode Miltzow; Fønhus, Marita Sporstøl; Haugen, Linda Hofstad; Eskild, Winnie & Berg, Trond [Show all 7 contributors for this article] (2019). Receptor-mediated endocytosis of VEGF-A in rat liver sinusoidal endothelial cells. BioMed Research International. ISSN 2314-6133. 2019, p. 1–12. doi: 10.1155/2019/5496197. Full text in Research Archive
• Nesset, Cecilie Kåsi; Kong, Xiang Yi; Damme, Markus; Schjalm, Camilla; Roos, Norbert & Løberg, Else Marit [Show all 7 contributors for this article] (2016). Age-dependent development of liver fibrosis in Glmp (gt/gt) mice. Fibrogenesis & Tissue Repair. ISSN 1755-1536. 9(5). doi: 10.1186/s13069-016-0042-4.
• Kong, Xiang Yi; Feng, Yuan Zeng; Eftestøl, Einar; Kase, Eili Tranheim; Haugum, Hanne & Eskild, Winnie [Show all 8 contributors for this article] (2016). Increased glucose utilization and decreased fatty acid metabolism in myotubes from Glmpgt/gt mice. Archives of Physiology and Biochemistry. ISSN 1381-3455. 122(1), p. 36–45. doi: 10.3109/13813455.2015.1120752.
• Kong, Xiang Yi; Kase, Eili Tranheim; Herskedal, Anette; Schjalm, Camilla; Damme, Markus & Nesset, Cecilie Kåsi [Show all 9 contributors for this article] (2015). Lack of the lysosomal membrane protein, GLMP, in mice results in metabolic dysregulation in liver. PLOS ONE. ISSN 1932-6203. 10(6). doi: 10.1371/journal.pone.0129402. Full text in Research Archive
• Kong, Xiang Yi; Nesset, Cecilie Kåsi; Damme, Markus; Løberg, Else Marit; Lübke, Torben & Mæhlen, Jan [Show all 13 contributors for this article] (2014). Loss of lysosomal membrane protein NCU-G1 in mice results in spontaneous liver fibrosis with accumulation of lipofuscin and iron in Kupffer cells. Disease Models and Mechanisms. ISSN 1754-8403. 7(3), p. 351–362. doi: 10.1242/dmm.014050. Full text in Research Archive
• Steffensen, Knut Rune; Bouzga, Mariam; Skjeldal, Frode; Kåsi, Cecilie; Karahasan, Almira & Matre, Vilborg [Show all 9 contributors for this article] (2007). Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator. BMC Molecular Biology. ISSN 1471-2199. 8(1), p. 106–120.
• Sporstol, Marita; Mousavi, Darbi Seyed Ali; Eskild, Winnie; Roos, Norbert & Berg, Trond (2007). ABCAI, ABCGI and SR-BI: hormonal regulation in primary rat hepatocytes and human cell lines. BMC Molecular Biology. ISSN 1471-2199. 8. doi: 10.1186/1471-2199-8-5.
• Malerød, Lene; Sporstol, Marita; Juvet, Lene Kristine; Mousavi, Darbi Seyed Ali; Gjøen, Tor & Berg, Trond [Show all 8 contributors for this article] (2005). Bile acids reduce SR-BI expression in hepatocytes by a pathway involving FXR/RXR, SHP, and LRH-1. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 336(4), p. 1096–1105.
• Steffensen, Knut Rune; Holter, Elin; Alikhani, N*; Eskild, Winnie & Gustafsson, Jan Åke (2003). Glucocorticoid response and promoter occupancy of the mouse LXRalpha gene. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 312(3), p. 716–724. doi: 10.1016/j.bbrc.2003.10.174.
• Moe-Behrens, GH; Klinger, Francesca G*; Eskild, Winnie; Grotmol, Tom; Haugen, Trine Berit & Felici, M De (2003). Akt/PTEN signaling mediates estrogen-dependent proliferation of primordial germ cells in vitro. Molecular Endocrinology. ISSN 0888-8809. 17(12), p. 2630–2638.
• Malerød, Lene; Juvet, Lene Kristine; Hanssen-Bauer, Audun; Eskild, Winnie & Berg, Trond (2002). Oxysterol-activated LXR-alpha/RXR induces hSR-BI-promoter activity in hepatoma cells and preadipocytes. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 299, p. 916–923.
• Steffensen, Knut Rune; Schuster, Gertrud U.; Parini, Paola; Holter, Elin; Sadek, Christine M. & Cassel, Tobias [Show all 8 contributors for this article] (2002). Different regulation of the LXR-alpha promoter activity by isoforms of CCAAT/enhancer-binding proteins. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 293, p. 1333–1340. doi: 10.1016/S0006-291X(02)00390-X.
• Steffensen, Knut Rune; Holter, Elin; Tobin, Kari Anne Risan; Leclerc, Steeve; Gustafsson, Jan Åke & Guérin, Sylvain L. [Show all 7 contributors for this article] (2001). Members of the nuclear factor 1 family reduce the transcriptional potential of the nuclear receptor LXR-alpha promoter. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 289, p. 1262–1267. doi: 10.1006/bbrc.2001.6078.
• Behrens, Gerd Heinz Gerhard & Eskild, Winnie (2000). Mus musculus Steel gene 5' flanking region. GenBank. ISSN 1080-742X.
• Behrens, Gerd Heinz Gerhard & Eskild, Winnie (2000). Mus musculus Steel gene 5' flanking region. GenBank. ISSN 1080-742X.
• Grønning, Line Mariann; Knutsen, Helle Katrine; Eskild, Winnie; Hansson, Vidar; Tasken, Kjetil & Taskén, Kristin Austlid (1999). Method of transfection affects the cAMP-mediated induction of the RII-beta subunit of protein kinase A in Sertoli cells: inhibition of response by increase in intracellular calcium. European Journal of Endocrinology (EJE). ISSN 0804-4643. 141, p. 75–82.
• Trøen, Gunhild; Eskild, Winnie; Fromm, Sigurd; Luca, Luigi M. De ; Ong, David E. & Wardlaw, Sarah A. [Show all 8 contributors for this article] (1999). Vitamin A sensitive tissues in transgenic mice expressing high levels of human cellular retinol-binding protein type I are not altered phenotypically. Journal of Nutrition. ISSN 0022-3166. 129, p. 1621–1627.
• Ree, Anne Hansen; Hansson, Vidar; Walaas, Sven Ivar; Eskild, Winnie & Taskén, Kristin Austlid (1999). Calcium/phospholipid-dependent protein kinases in rat Sertoli cells: regulation of androgen receptor messenger ribonucleic acid. Biology of Reproduction. ISSN 0006-3363. 60, p. 1257–1262.
• Knutsen, Helle Katrine; Tasken, Kjetil; Eskild, Winnie; Richards, J.S.; Kurten, R.C. & Torjesen, Peter A. [Show all 10 contributors for this article] (1997). Characterization of the 5'-flanking region of the gene for the cAMP-inducible protein kinase A subunit, RIIbeta, in Sertoli cells. Molecular and Cellular Endocrinology. ISSN 0303-7207. 129(1), p. 101–114.
• Leclerc, S.; Eskild, Winnie & Guérin, Sylvain L. (1997). The rat growth hormone and human cellular retinol binding protein1 genes share homologous NF1-like binding sites that exert either positive or negative influences on gene exprssion in vivo. DNA and Cell Biology. ISSN 1044-5498. 16, p. 951–967.
• Knutsen, Helle Katrine; Reinton, Nils Torgny Kjelkevik; Taskén, Kristin Austlid; Hansson, Vidar & Eskild, Winnie (1996). Induction of RIIbeta mRNA is independent of continuous protein synthesis. Biology of Reproduction. ISSN 0006-3363. 55, p. 5–10.
• Knutsen, Helle Katrine; Reinton, Nils Torgny Kjelkevik; Taskén, Kristin Austlid; Hansson, Vidar & Eskild, Winnie (1996). Induction of RIIbeta mRNA is independent of continuous protein synthesis. Biology of Reproduction. ISSN 0006-3363. 55, p. 5–10.
• Knutsen, Helle Katrine; Reinton, Nils Torgny Kjelkevik; Taskén, Kristin Austlid; Hansson, Vidar & Eskild, Winnie (1996). Induction of RIIbeta mRNA is independent of continuous protein synthesis. Biology of Reproduction. ISSN 0006-3363. 55, p. 5–10.
• Kroepelien, Christian Fredrik; Knutsen, Helle Katrine; Haugen, Trine B.; Hansson, Vidar & Eskild, Winnie (1993). Serum factors induce messenger ribonucleic acid levels for cellular retinol-binding protein in rat Sertoli cells. Endocrinology. ISSN 0013-7227. 132(3), p. 968–974.
• Ree, Anne Hansen; Knutsen, H. K.; Landmark, Brynjar Fowels; Eskild, Winnie & Hansson, Vidar (1992). Down-regulation of messenger ribonucleic acid (mRNA) for the estrogen receptor (ER) by phorbol ester requires ongoing RNA synthesis but not protein synthesis. Is hormonal control of ER mRNA degradation mediated by an RNA molecule? Endocrinology. ISSN 0013-7227. 131, p. 1810–1814.
• Knutsen, Helle Katrine; Tasken, Kristin Austlid; Eskild, Winnie; Jahnsen, Tore & Hansson, Vidar (1992). Half-lives of different sized mRNAs for the PKA subunit RI alpha are regulated differently in response to inhibition of transcription and translation. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 184(1), p. 454–460.
• Tasken, Kristin Austlid; Knutsen, Helle Katrine; Eikvar, Lars Kristian; Tasken, Kjetil; Eskild, Winnie & Jahnsen, Tore [Show all 7 contributors for this article] (1992). Protein kinase C activation by 12-O-tetradecanoylphorbol 13-acetate modulates messenger ribonucleic acid levels for two of the regulatory subunits of 3',5'-cyclic adenosine monophosphate-dependent protein kinases (RII beta and RI alpha) via multiple and distinct mechanisms. Endocrinology. ISSN 0013-7227. 130(3), p. 1271–1280.
• Haugen, Trine B.; Eskild, Winnie & Hansson, Vidar (1992). Evidence for a novel splice variant of the alpha subunit of Go in rat male haploid germ cells. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 183(1), p. 41–47.
• Landmark, Brynjar Fowels; Fauske, Beathe; Eskild, Winnie; Skålhegg, Bjørn Steen; Lohmann, Suzanne M. & Hansson, Vidar [Show all 8 contributors for this article] (1991). Identification, Characterization, and Hormonal Regulation of 3′,5′-Cyclic Adenosine Monophosphate Dependent Protein Kinases in Rat Sertoli Cells. Endocrinology. ISSN 0013-7227. 129(5), p. 2345–2354.
• Eskild, Winnie; Ree, Anne Hansen; Levy, Finn Olav; Jahnsen, Tore & Hansson, Vidar (1991). Cellular localization of mRNAs for the retinoic acid receptor-alpha, cellular retinol-binding protein, and cellular retinoic acid-binding protein in rat testis: evidence for germ cell-specific mRNAs. Biology of Reproduction. ISSN 0006-3363. 44, p. 53–61.
• Ree, Anne Hansen; Landmark, Brynjar Fowels; Walaas, Sven Ivar; Lahooti, H.; Eikvar, Lars & Eskild, Winnie [Show all 7 contributors for this article] (1991). Down-regulation of messenger ribonucleic acid and protein levels for estrogen receptors by phorbol ester and calcium in MCF-7 cells. Endocrinology. ISSN 0013-7227. 129, p. 339–344.
• Knutsen, Helle Katrine; Tasken, Kristin Austlid; Eskild, Winnie; Jahnsen, Tore & Hansson, Vidar (1991). Adenosine 3',5'-monophosphate-dependent stabilization of messenger ribonucleic acids (mRNAs) for protein kinase-A (PKA) subunits in rat Sertoli cells: rapid degradation of mRNAs for PKA subunits is dependent on ongoing RNA and protein synthesis. Endocrinology. ISSN 0013-7227. 129(5), p. 2496–2502.
• Tasken, Kristin Austlid; Knutsen, Helle Katrine; Attramadal, Håvard; Tasken, Kjetil; Jahnsen, Tore & Hansson, Vidar [Show all 7 contributors for this article] (1991). Different Mechanisms are Involved in cAMP Mediated Induction of mRNAs for Subunits of cAMP-Dependent Protein Kinases. Molecular Endocrinology. ISSN 0888-8809. 5(1), p. 21–28.
• Ree, Anne Hansen; Frøysa, A.; Eskild, Winnie; Jahnsen, Tore & Hansson, Vidar (1990). Biphasic regulation of the messenger ribonucleic acid coding for the estrogen receptor by cyclic adenosine 3`:5`-monophosphate in tumor Leydig cells. Cancer Research. ISSN 0008-5472. 50, p. 1528–1531.
• Ree, Anne Hansen; Landmark, Brynjar Fowels; Eskild, Winnie; Levy, Finn Olav; Lahooti, H. & Jahnsen, Tore [Show all 8 contributors for this article] (1989). Autologous down-regulation of messenger ribonucleic acid and protein levels for estrogen receptors in MCF-7 cells: an inverse correlation to progesterone receptor levels. Endocrinology. ISSN 0013-7227. 124, p. 2577–2583.
• Eskild, Winnie; Kindberg, G. M.; Smedsrød, Bård; Blomhoff, Rune; Norum, K. R. & Berg, Trond (1989). Intracellular transport of formaldehyde treated serum albumin in liver endothelial cells after uptake via scavenger receptors. Biochemical Journal. ISSN 0264-6021. 258, p. 511–520.
• Yoganathan, T.; Øyen, Ole; Eskild, Winnie; Jahnsen, Tore & Hansson, Vidar (1989). Cellular localization and age dependent changes in mRNA for glutathione S-transferase-P in rat testicular cells. Biochemistry International. ISSN 0158-5231. 19(4), p. 667–672.
• Øyen, Ole; Eskild, Winnie; Jahnsen, Tore & Hansson, Vidar (1989). FSH, cAMP and gene regulation in testis. In Serio, Mario (Eds.), Perspectives in Andrology. Wolters Kluwer. ISSN 088167561X. p. 259–269.
• Levy, Finn Olav; Øyen, Ole; Sandberg, Mårten; Tasken, Kjetil; Eskild, Winnie & Hansson, Vidar [Show all 7 contributors for this article] (1988). Molecular cloning, complementary deoxyribonucleic acid structure and predicted full-length amino acid sequence of the hormone-inducible regulatory subunit of 3'-5'-cyclic adenosine monophosphate-dependent protein kinase from human testis. Molecular Endocrinology. ISSN 0888-8809. 2(12), p. 1364–1373.
• Øyen, Ole; Eskild, Winnie; Beebe, Stephen; Hansson, Vidar & Jahnsen, Tore (1988). Biphasic response to 3',5'-cyclic adenosine monophosphate (cAMP) at the messenger ribonucleic acid level for a regulatory subunit of cAMP-dependent protein kinase. Molecular Endocrinology. ISSN 0888-8809. 2(11), p. 1070–1076.
• Øyen, Ole; Sandberg, Mårten; Eskild, Winnie; Levy, Finn Olav; Knutsen, Gro & Beebe, Stephen [Show all 8 contributors for this article] (1988). Differential regulation of messenger ribonucleic acids for specific subunits of cyclic adenosine 3',5'-monophosphate cAMP)-dependent protein kinase by cAMP in rat Sertoli cells. Endocrinology. ISSN 0013-7227. 122(6), p. 2658–2666.
• Eskild, Winnie; Øyen, Ole; Beebe, Stephen; Jahnsen, Tore & Hansson, Vidar (1988). Regulation of mRNA levels for cellular retinol binding protein in rat Sertoli cells by cyclic AMP and retinol. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 152(3), p. 1504–1510.
• Øyen, Ole; Eskild, Winnie; Frøysa, Anneke; Sandberg, Mårten; Levy, Finn Olav & Knutsen, Gro [Show all 8 contributors for this article] (1987). Hormonal regulation and age dependent changes in mRNA levels for regulatory subunits of cAMP-dependent protein kinases in rat testis. In Orgebin-Crist, M.-C. & Danzo, B. J. (Ed.), Cell Biology of the Testis and Epididymis. New York Academy of Sciences. ISSN 0897664213. p. 473–476.
• Øyen, Ole; Frøysa, Anneke; Sandberg, Mårten; Eskild, Winnie; Joseph, D. & Hansson, Vidar [Show all 7 contributors for this article] (1987). Cellular localization and age-dependent changes in mRNA for cyclic adenosine 3',5'-monophosphate-dependent protein kinases in rat testis. Biology of Reproduction. ISSN 0006-3363. 37(4), p. 947–956.
• Smedsrød, Bård; Pertoft, Håkan; Kjellén, Lena; Johansson, Staffan; Eskild, Winnie & Laurent, Torvard C. (1986). Endocytosis of connective tissue macromolecules by liver endothelial cells. In Kirn, A; Knook, D.L. & Wisse, E (Ed.), In Cells of the hepatic sinusoid. Pasmans, Netherlands. p. 245–250.

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• Eskild, Winnie; May, Gerhard; Michelangeli, Frank; Minchin, Steve; Roberts, Mark & Saðýn, Ferhan [Show all 9 contributors for this article] (2021). Meeting reports: Evolving Molecular Bioscience Education 2021. Biochemist. ISSN 0954-982X. 43(4), p. 49–49. doi: 10.1042/bio_2021_160.
• Sørensen, Karen Kristine; Antwi, Milton Boaheng; Dumitriu, Gianina; Li, Ruomei; Smedsrød, Bård & Eskild, Winnie (2019). Scavenger function of liver sinusoidal endothelial cells in a transgenic mouse model of liver fibrosis is reduced.
• Zhou, Yun; Corbin, Karen; Xiang, Yi-Kong; Eskild, Winnie; Zeisel, Steven H. & Danbolt, Niels Christian (2015). The betaine-GABA transporter (BGT1; slc6a12) is not involved in the inactivation of transmitter GABA, but plays a role in preserving the choline levels in the liver.
• Zhou, Yun; Corbin, Karen; Xiang, Yi-Kong; Eskild, Winnie; Zeisel, Steven H. & Danbolt, Niels Christian (2015). The betaine-GABA transporter (BGT1; slc6a12) may play its main roles in the liver rather than in the brain .
• Kong, Xiang Yi; Nesset, Cecilie Kåsi; Schjalm, Camilla; Løberg, Else Marit; Rustan, Arild & Thoresen, G. Hege [Show all 8 contributors for this article] (2014). NCU-G1GT/GT MICE: A LONG-LIVED MODEL FOR LIVER FIBROSIS.
• Schalm, Camilla; Kong, Xiang Ying; Kaasi, Cecilie & Eskild, Winnie (2013). THE NCU-G1gt/gt MOUSE AS A LIVER FIBROSIS MODEL ORGANISM: A STUDY OF THE DEVELOPMENT OF FIBROSIS AND RESPONSE TO ANTI-FIBROTIC TREATMENT.
• Kong, Xiang Yi; Kasi, C; Schjalm, C.; Loberg, EM; Rustan, A.C. & Thoresen, G. Hege [Show all 8 contributors for this article] (2012). NCU-G1 knock-out mice: a potential new model for liver fibrosis. The FEBS Journal. ISSN 1742-464X. 279, p. 573–573.
• Lystad, Alf Håkon; Sporstol, Marita; Foroughi, Masoud; Roos, Norbert; Berg, Trond & Eskild, Winnie (2009). Regulation of VEGF expression by vitamin D i hepatic stellate cells - possible attenuation by the nuclear receptor coregulator NCY-G1.
• Lystad, Alf Håkon; Sporstol, Marita; Foroughi, Masoud; Roos, Norbert; Berg, Trond & Eskild, Winnie (2009). Regulation of VEGF expression by vitamin D in hepatic stellate cells - possible attenuation by the nuclear receptor coregulator NCU-G1.
• Kåsi, Cecilie; Sporstol, Marita; Roos, Norbert; Berg, Trond & Eskild, Winnie (2007). NUCLEAR RECEPTORS IN HEPATIC STELLATE CELL ACTIVATION.
• Eskild, Winnie & Andersson, Kristin Brevik (2007). Conditional knock-out of the NCU-G1 gene.
• Enger, Martin a*; Bouzga, Mariam; Steffensen, Knut Rune; Guerin, Sylvain L & Eskild, Winnie (2005). Human NCU-G1 is a transcription factor and a nuclear receptor coactivator.
• Enger, Martin DV; Bouzga, Mariam; Steffensen, Knut Rune; Guerin, Sylvain L & Eskild, Winnie (2005). Human NCU-G1 is a transcription factor and a nuclear receptor coactivator.
• Orby, Richard; Enger, Martin a*; Bouzga, Mariam & Eskild, Winnie (2004). Human NCU-G1 - a possible multifunctional nuclear protein.
• Enger, Martin a*; Orby, Richard & Eskild, Winnie (2004). NCU-G1 is essential for cell survival.
• Steffensen, Knut Rune; Enger, Martin A.; Bouzga, Mariam; Guérin, Sylvain L & Eskild, Winnie (2004). Human NCU-G1 is a transcription factor and a coactivator of nuclear receptors.
• Bouzga, Mariam; Steffensen, Knut Rune & Eskild, Winnie (2003). A novel transcription factor interacts with the CRBP1 promoter.
• Sarre, Aili; Enger, Martin A; Steffensen, Knut Rune; Bouzga, Mariam & Eskild, Winnie (2003). Act#7 - a novel transcription factor.
• Steffensen, Knut Rune; Holter, Elin & Eskild, Winnie (1999). TPA, an activator of protein kinase C, reduces transcriptional activation of the LXR-alfa gene.
• Eskild, Winnie (1997). Mat og modifiserte gener - Er det farlig å spise gener?
• Sørensen, H. N.; Steineger, Hilde Hermansen; Eskild, Winnie; Gautvik, Kaare M; Spydevold, Øystein & Gustafsson, Jan Åke (1997). Fatty acid and hormonal regulation of orphan receptors that heterodimerize with retionoic acid receptor_a (RXR_a).
• Aschim, Elin Leirvoll; Østvold, Anne Carine & Eskild, Winnie (1997). Regulation of CRBP1 function.
• Andersen, A.K.; Haugen, T. B. & Eskild, Winnie (1997). Expression and regulation of PPAR_b and RXR_b in Sertoli cells.
• Eskild, Winnie (1996). Regulation of CRBP1 expression and function in Sertoli cells.
• Eskild, Winnie (1996). Regulation of CRBP1 expression and function in Sertoli cells.
• Taskén, Kristin Austlid; Grønning, Line Mariann; Knutsen, Helle Katrine; Tasken, Kjetil; Eskild, Winnie & Hansson, Vidar (1996). Maintenance of cAMP-responsive gene transcription during transient transfection of primary cultures of rat Sertoli cells.
• Eskild, Winnie; Trøen, Gunhild; Blaner, W.S.; Nilsson, A. & Hansson, Vidar (1996). Cellular retinol binding protein 1 in rat Sertoli cells - Evidence for independent control at the mRNA and protein levels.
• Taskén, Kristin Austlid; Grønning, Line Mariann; Knutsen, Helle Katrine; Eskild, Winnie & Hansson, Vidar (1996). Maintenance of cAMP-responsive gene transcription during transient transfection of primary cultures of rat Sertoli cells.
• Eskild, Winnie & Østvold, Anne Carine (1996). Regulation of CRBP1 activity - Possible involvement of protein kinase C.
• Landmark, Brynjar Fowels; Eskild, Winnie; Lohmann, Suzanne M.; Walaas, S. Ivar; Jahnsen, Tore & Hansson, Vidar (1990). Protein kinases in rat testis and MCF-7 cells.
• Eskild, Winnie; Ree, Anne Hansen; Jahnsen, Tore & Hansson, Vidar (1989). Localization and age-dependent changes in mRNA for cellular retinoic acid binding protein and retinoic acid receptor in testis.
• Landmark, Brynjar Fowels; Eskild, Winnie; Doskeland, Stein Ove; Skålhegg, Bjørn Steen; Lohmann, Susanne & Walter, Ulrich [Show all 9 contributors for this article] (1989). Characterization and hormonal regulation of cAMP-dependent protein kinase in rat Sertoli cells.
• Eskild, Winnie; Ree, Anne Hansen; Jahnsen, Tore & Hansson, Vidar (1988). Localization and age dependent changes in mRNA for cellular retinoic acid protein and retinoic acid receptor in testis.
• Eskild, Winnie; Øyen, Ole; Beebe, Stephen; Jahnsen, Tore & Hansson, Vidar (1988). Regulation of mRNA for cellular retinol binding protein in cultured rat Sertoli cells.
• Jahnsen, Tore; Øyen, Ole; Sandberg, Mårten; Levy, Finn Olav; Eskild, Winnie & Frøysa, Anneke [Show all 7 contributors for this article] (1988). Differential regulation of mRNA for specific subunits of cAMP-dependent protein kinase by FSH and cAMP in Sertoli cells.
• Eskild, Winnie; Øyen, Ole; Beebe, Stephen; Jahnsen, Tore & Hansson, Vidar (1988). Expression of mRNA for cellular retinol binding protein in cultured rat Sertoli cells: Regulation by cAMP and retinol.
• Levy, Finn Olav; Øyen, Ole; Sandberg, Mårten; Tasken, Kjetil; Eikvar, Lars Kristian & Eskild, Winnie [Show all 8 contributors for this article] (1988). Stimulatory effects of glucocorticoids on mRNA levels for the hormone-induced regulatory subunit (RIIbeta) of cAMP-dependent protein kinase and ABP in cultured immature rat Sertoli cells; Molecular cloning of a fulllength cDNA for RIIbeta from human testis.
• Ree, Anne Hansen; Eskild, Winnie; Levy, Finn Olav; Eikvar, Lars Kristian; Jahnsen, Tore & Hansson, Vidar (1988). Developmental patterns of mRNA levels for estrogen and glucocorticoid receptors in rat testis.
• Eskild, Winnie; Øyen, Ole; Beebe, Stephen; Jahnsen, Tore & Hansson, Vidar (1988). Regulation of mRNA for cellular retinol binding protein in rat Sertoli cells.
• Landmark, Brynjar Fowels; Beebe, Stephen; Øyen, Ole; Eskild, Winnie & Jahnsen, Tore (1988). Characterization and cAMP regulation of cAMP-dependent protein kinase subunits in rat Sertoli cells. Show summary

  Poster Serono Symposia publications from Raven Press, United States New York:1988. 336 p ISBN 9780881674187

• Ree, Anne Hansen; Landmark, Brynjar Fowels; Eskild, Winnie; Levy, Finn Olav & Hansson, Vidar (1988). Effects of estradiol on mRNA levels for steroid receptors in human mammary carcinoma cells (MCF-7). Show summary

  Abstract #56

• Øyen, Ole; Frøysa, Anneke; Eskild, Winnie; Sandberg, Mårten; Hansson, Vidar & Jahnsen, Tore (1987). Specific expression of mRNA's for regulatory subunits of cAMP-dependent protein kinase during differentiation and hormonal stimulation in rat testis.
• Eskild, Winnie; Øyen, Ole; Levy, Finn Olav; Frøysa, Anneke; Sandberg, Mårten & Eriksson, U. [Show all 10 contributors for this article] (1987). Cellular localization and regulation in testis of mRNA for Cellular Retinol Binding Protein.
• Øyen, Ole; Sandberg, Mårten; Eskild, Winnie; Frøysa, Anneke; Hansson, Vidar & Jahnsen, Tore (1987). Induction of cell specific mRNA's for regulatory subunits of cAMP-dependent protein kinase in rat germinal and Sertoli cells.
• Eskild, Winnie; Øyen, Ole; Jahnsen, Tore & Hansson, Vidar (1987). Inhibition of mRNA for Cellular Retinol Binding Protein (CRBP) in Sertoli cells by dibutyryl-cAMP.
• Jahnsen, Tore; Øyen, Ole; Beebe, Stephen; Sandberg, Mårten; Frøysa, Anneke & Eskild, Winnie [Show all 9 contributors for this article] (1987). Regulation of hormonal responses at the level of cAMP-dependent protein kinases.
• Øyen, Ole; Eskild, Winnie; Sandberg, Mårten; Frøysa, Anneke; Knutsen, G. & OPSAHL, G. [Show all 9 contributors for this article] (1987). Cell specific expression of small-sized mRNA's for regulatory subunits of cAMPdependent protein kinase during germ cell differentiation.
• Eskild, Winnie; Øyen, Ole; Levy, Finn Olav; Frøysa, Anneke; Sandberg, Mårten & Attramadal, Håvard [Show all 8 contributors for this article] (1987). Cellulær lokalisasjon og hormonell regulering i testis av mRNA for cellulært retinol (Vit A) bindende protein.
• Berg, Trond; Kindberg, Grete Mørk; Andersen, Knut-Jan; Eskild, Winnie & Norum, Kaare Reidar (1986). Receptor-Mediated Endocytosis in Isolated Rat Liver Perenchymal and Nonparenchymal Cells, Studied by Means of Subcellular Fractionation in Nycodenz and Sucrose Gradients.
• Øyen, Ole; Eskild, Winnie; Attramadal, Håvard; Gladhaug, Ivar Prydz; Knutsen, G. & Richards, JoAnne S. [Show all 8 contributors for this article] (1986). Hormonal regulation of mRNA for the regulatory subunit (R-II51) of type II cAMP-dependent protein kinase in rat Sertoli cells.
• Øyen, Ole; Eskild, Winnie; Attramadal, Håvard; Gladhaug, Ivar Prydz; Levy, Finn Olav & Knutsen, G. [Show all 9 contributors for this article] (1986). Cyclic AMP increases the mRNA levels for the regulatory subunit (R-II51) of type II cAMP-dependent protein kinase in rat Sertoli cells.
• Hassan, Liza Hamdi Mohamed; Flengsrud, Ragnar & Eskild, Winnie (2011). Verification of two potential glycosylation sites in human NCU-G1. Universitetet for miljø og biovitenskap,. Show summary

  Glycosylation is an important posttranslational modification the greatly affects protein function. N-glycosylation is one class of this modification that has been characterized widely for some proteins. This glycosylation type involves the addition of glycans to asparagine amino acids. The most common acceptor motif for N-glycosylation is N-X-S/T. NCU-G1 is a highly glycosylated novel protein. According to mentioned consensus motif, hNCU-G1 contains seven predicted glycosylation sites. The aim of this study was to investigate some of the potential glycosylation sites on the hNCU-G1 amino sequence and to study the effect of these sites on the protein molecular weight, expression and subcellular localization. Although prediction programs gave variable results concerning the potential glycosylation sites on the hNCU-G1 protein sequence, they agreed on four of these sites. We changed the asparagine amino acid of two of these sites to alanine using a site directed mutagenesis kit. These sites were located at positions 65 and 230 of the hNCU-G1 amino acid sequence. We transiently transfected HeLa cells with the wild type hNCU-G1 and the two N-glycosylation mutants (65mut and 230mut), then analyzed the expressed protein by western immunoblotting and confocal microscopy. Both methods showed lower expression of the65mut, as opposed to a very high expression of the 230mut. Two glycoforms of NCU-G1proteins with the molecular weight of 62 and 75 kDa were detected. In addition, the230mut was found to lower the apparent molecular weight of the 75 kDa glycoform by around 3 kDa. hNCU-G1 was found to localize in the cytoplasm, and it was also detected in the nuclei of the 230mut. In conclusion, we hypothesize that glycosylation at position 65 of the hNCU-G1 amino acid sequence possibly affects its stability, folding, or antigenicity and that glycosylation at position 230 possibly affects its antigenicity, turnover or conformation. In addition, this glycosylation site influences the nuclear import, although this effect is not well understood. Finally, we expect that hNCU-G1 has more than one glycoform, with molecular weights that are cell and organelle specific. To this end, we recommend further characterization of more single and multiple glycosylation mutants. It would be of great value to study the exact mechanisms by which the glycosylation mutants exert their effects.

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