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Our researchers in the media
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Crystal structure of an L chain optimised 14F7 anti-ganglioside Fv suggests a unique tumour-specificity through an unusual H-chain CDR3 architecture (Nature)
July 18, 2018 11:03 AM
Targeted cancer immunotherapy offers increased efficacy concomitantly with reduced side effects. One antibody with promising clinical potential is 14F7, which specifically recognises the NeuGc GM3 ganglioside. This antigen is found in the plasma membrane of a range of tumours, but is essentially absent from healthy human cells. 14F7 can discriminate NeuGc GM3 from the very similar NeuAc GM3, a common component of cell membranes. The molecular basis for this unique specificity is poorly understood. Here we designed and expressed 14F7-derived single-chain Fvs (scFvs), which retained the specificity of the parent antibody. Detailed expression and purification protocols are described as well as the synthesis of the NeuGc GM3 trisaccharide. The most successful scFv construct, which comprises an alternative variable light chain (V LA), allowed structure determination to 2.2 Å resolution. The structure gives insights into the conformation of the important CDR H3 loop and the suspected antigen binding site. Furthermore, the presence of V LAinstead of the original V Lelucidates how this subdomain indirectly stabilises the CDR H3 loop. The current work may serve as a guideline for the efficient production of scFvs for structure determination.
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Biogeographic regions and events of isolation and diversification of the endemic biota of the tropical Andes (PNAS)
July 17, 2018 5:04 PM
Nicolas A. Hazzi, Juan Sebastián Moreno, Carolina Ortiz-Movliav, and
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Multidecadal, county-level analysis of the effects of land use, Bt cotton, and weather on cotton pests in China (PNAS)
July 16, 2018 9:45 PM
Wei Zhang, Yanhui Lu, Wopke van der Werf, Jikun Huang, Feng Wu, Ke Zhou,
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Multidecadal, county-level analysis of the effects of land use, Bt cotton, and weather on cotton pests in China (PNAS)
July 16, 2018 9:01 PM
Wei Zhang, Yanhui Lu, Wopke van der Werf, Jikun Huang, Feng Wu, Ke Zhou,
News
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Innovation at UiO: Six teams ready for SPARK Norway
Feb. 1, 2018 1:14 PM
Six new teams have been admitted to UiO´s innovation programme SPARK Norway. They will develop their ideas within health-related life sciences for the benefit of patients and society.
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Milad H. Mobarhan talks about understanding the brain with physics (in Norwegian).
Jan. 24, 2018 3:07 PM
Hva kan fysikere bidra med i hjerneforskning? Ph.D student Milad H. Mobarhan gir elever fra den videregående skole et innblikk i dette i sitt glimrende foredrag under universitetets åpen dag. Se forelesningen her.
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Vacancies
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Postdoctoral Research Fellowship in Population Genomics/Comparative Immunogenomics
Department: Centre for Ecological and Evolutionary Synthesis (CEES)Faculty: The Faculty of Mathematics and Natural SciencesLanguages: EnglishDeadline: Wednesday, August 8, 2018
Please note that vacancies announced in Norwegian only appear at the Norwegian website.
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