awrobel

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Academic interests

Yersinia ruckeri is a Gram-negative bacterium pathogen responsible for enteric redmouth disease. Yersinia ruckeri infections cause significant economic losses in salmonid aquaculture. Research into the pathogenicity of Yersinia ruckeri is limited.

The group of proteins, that mediate the fish-pathogen interaction comprise adhesins anchored in the bacterial outer membrane. They are essential for infection as they possess the ability to bind a variety of host molecules.

The aim of my research is to understand the molecular mechanisms of adhesion and to identify adhesins of interest and their cellular receptors in order to assess their function in pathogenesis.To examine whether adhesins of interest are involved in Yersinia ruckeri virulence, fish cell line and in vivo system (zebrafish embryos) will be used and optimised. Fluorescence microscopy will allow to visualize infections to assess the role of virulence genes. Infection of colour- fluorescent bacteria will be essential to follow the infection.

Courses taught

Background

I graduated from Poznań University of Life Sciences in 2011 with a Master of Science Engineer in Biotechnology. This degree has a large emphasis on Microbiology and Molecular Biology. From July to September 2011 I worked in the Molecular Diagnostic Research Group, Microbiology, NUIG, Galway. Here I had a great opportunity to develop a real time NASBA assay, which helped me understand the specifics of molecular biology. From January to December 2013 I worked with a dynamic and expanding Microbiology group based at University College Cork, Ireland. During this time I learned various bioengineering methods  such as molecular cloning.

 

 

Publications

"Expanding the biotechnology potential of lactobacilli through comparative genomics"

Z.Sun,H.M.B. Harris, A. McCann, X. Yang, S. Argimon, W. Zhang, Ch. Guo, I. B. Jeffery, J. C. Cooney,T. F. Kagawa, W. Liu, Y. Song, E. Salvetti, A. Wrobel, P. Rasinkangas, J. Parkhill, M. C. Rea, O. O’Sullivan, J. Ritari, F. P. Douillard, R. P. Ross, R. Yang, A. Briner, G. E. Felis, W. M. de Vos, R. Barrangou, T. R. Klaenhammer, P. W. Caufield, Y. Cui, H. Zhang, P. W.O’Toole. Nature Communications. 2014.

  • Antenucci, Fabio; Ovsepian, Armen; Wróbel, Agnieszka Ewelina; Winther-Larsen, Hanne Cecilie & Bojesen, Anders Miki (2020). Design and characterization of a novel tool for the antigenic enrichment of actinobacillus pleuropneumoniae outer membrane. Pathogens. ISSN 2076-0817. 9(12). doi: 10.3390/pathogens9121014.
  • Wróbel, Agnieszka Ewelina; Saragliadis, Athanasios; Pérez-Ortega, Jesús; Sittman, Carolin; Göttig, Stephan & Liskiewicz, Krystyna Anna [Show all 11 contributors for this article] (2020). The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence. Environmental Microbiology. ISSN 1462-2912. 22(7), p. 2939–2955. doi: 10.1111/1462-2920.15051. Full text in Research Archive
  • Wróbel, Agnieszka Ewelina; Leo, Jack Christopher & Linke, Dirk (2019). Overcoming fish defences: The virulence factors of Yersinia ruckeri. Genes. ISSN 2073-4425. 10(9), p. 1–20. doi: 10.3390/genes10090700. Full text in Research Archive
  • Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher & Linke, Dirk (2018). pYR4 From a Norwegian Isolate of Yersinia ruckeri Is a Putative Virulence Plasmid Encoding Both a Type IV Pilus and a Type IV Secretion System. Frontiers in Cellular and Infection Microbiology. ISSN 2235-2988. 8, p. 1–14. doi: 10.3389/fcimb.2018.00373. Full text in Research Archive
  • Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher; Gulla, Snorre & Linke, Dirk (2017). The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a “Y. ruckeri invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen. Journal of Structural Biology. ISSN 1047-8477. 201(2), p. 171–183. doi: 10.1016/j.jsb.2017.08.008.
  • Chauhan, Nandini; Wrobel, Agnieszka; Skurnik, Mikael & Leo, Jack Christopher (2016). Yersinia adhesins: An arsenal for infection. PROTEOMICS - Clinical Applications. ISSN 1862-8346. 10(9-10), p. 949–963. doi: 10.1002/prca.201600012.

View all works in Cristin

  • Wrobel, Agnieszka; Saragliadis, Athanasios; Leo, Jack Christopher; Ottoni, Claudio & Linke, Dirk (2018). Type Ve autotransporters in Yersinia ruckeri.
  • Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher & Linke, Dirk (2018). The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a “Y. ruckeri invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen.
  • Liskiewicz, Krystyna; Wrobel, Agnieszka; Leo, Jack Christopher & Linke, Dirk (2017). Identification of potential adhesin receptors from Yersinia ruckeri.
  • Wrobel, Agnieszka (2017). The repeat structure of two paralogous genes, Yersinia ruckeri Invasin (yrInv) and a “Y. ruckeri Invasin-like molecule”, (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen" .
  • Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher & Linke, Dirk (2017). IDENTIFICATION AND CHARACTERIZATION OF INVERSE AUTOTRANSPORTERS FROM A FISH PATHOGEN, YERSINIA RUCKERI.
  • Wrobel, Agnieszka; Leo, Jack Christopher; Ottoni, Claudio; Gulla, Snorre & Linke, Dirk (2017). Comparative structure-function analysis of adhesins from different Yersinia ruckeri strains.
  • Wrobel, Agnieszka & Linke, Dirk (2017). The repeat structure of two paralogous invasin genes from Yersinia ruckeri sheds light on the evolution of adhesive capacities of a Fish pathogen.
  • Wrobel, Agnieszka; Ottoni, Claudio; Leo, Jack Christopher & Linke, Dirk (2017). IDENTIFICATION AND CHARACTERIZATION OF INVERSE AUTOTRANSPORTERS FROM A FISH PATHOGEN, YERSINIA RUCKERI.
  • Wrobel, Agnieszka & Linke, Dirk (2016). Zebrafish embryos as a model system for studying Yersinia ruckeri infection.
  • Wrobel, Agnieszka & Linke, Dirk (2016). Zebrafish as a model system for studying Yersinia ruckeri infections.
  • Wrobel, Agnieszka & Linke, Dirk (2015). Comparative structure-function analysis of adhesin proteins from different Yersinia species.
  • Wrobel, Agnieszka & Linke, Dirk (2015). Zebrafish embryos as a model for studying Yersinia ruckeri infection.
  • Wrobel, Agnieszka & Linke, Dirk (2015). Optimization of a highly efficient electroporation system for construction of a green fluorescent protein-tagged Yersinia ruckeri.

View all works in Cristin

Published Apr. 27, 2015 8:27 AM - Last modified Sep. 7, 2015 2:41 PM

Research groups