Christian Koehler

Image of Christian Koehler
Norwegian version of this page
Phone +47-22840512
Username
Visiting address Blindernveien 31 None 0371 OSLO
Postal address Postboks 1066 Blindern 0316 OSLO

Academic interests

Proteomics, Bioinformatics

Courses taught

Background

 

  • Biotechnology Centre of Oslo
Doctor of Philosophy (PhD), Analytical Chemistry

Development of Isobaric Peptide Termini Labeling (IPTL), an alternative quantification technique for mass spectrometry based quantitative proteomics.

 

 

  • University of Applied Sciences, Bingen
Diplom Ingenieur (FH), Process engineering, Biotechnology
 

Awards

     

    Positions held

    • Head Engineer
    University of Oslo
    – Present (2 years 1 month)Oslo Area, Norway
     
     
    • Researcher
    Biotechnology Centre of Oslo
    (10 months)
     
     

     

    • PHD Student
    Biotechnology Centre of Oslo
    (3 years 4 months)University of Oslo

    Development of Isobaric Peptide Termini Labeling (IPTL), an alternative quantification technique for mass spectrometry based quantitative proteomics.

     
     
    • Principal Engineer
    Biotechnology Centre of Oslo
    (2 years)

    - Maintenance and operation of HPLC and mass spectrometry instrumentation.
    - Proteomics services.
    - Development and implementation of proteomics methods.

     

     
    • Research Engineer
    RCSI -Royal college of surgeons in Ireland
    (2 years)

    - Core engineer in the mass spectrometry lab.
    - Involvement in several proteomics projects.
    - Maintenance and operation of HPLC and mass spectrometry instrumentation.
    - Proteomics services

     

    Publications

    • Bollineni, Ravi Chand; Koehler, Christian; Gislefoss, Randi Elin; Anonsen, Jan Haug & Thiede, Bernd (2018). Large-scale intact glycopeptide identification by Mascot database search. Scientific Reports.  ISSN 2045-2322.  8 . doi: 10.1038/s41598-018-20331-2
    • Dugarte, Maria Eugenia Chollet; Andersen, Elisabeth; Skarpen, Ellen; Myklebust, Christiane Filion; Koehler, Christian; Morth, Jens Preben; Chuansumrit, Ampaiwan; Pinotti, Mirko; Bernardi, Francesco; Thiede, Bernd; Sandset, Per Morten & Skretting, Grethe (2018). Factor VII deficiency: Unveiling the cellular and molecular mechanisms underlying Three model alterations of the enzyme catalytic domain. Biochimica et Biophysica Acta - Molecular Basis of Disease.  ISSN 0925-4439.  1864(3), s 660- 667 . doi: 10.1016/j.bbadis.2017.12.016
    • Koehler, Christian; Bollineni, Ravi Chand & Thiede, Bernd (2017). Application of the half decimal place rule to increase the peptide identification rate. Rapid Communications in Mass Spectrometry.  ISSN 0951-4198.  31(2), s 227- 233 . doi: 10.1002/rcm.7780 Full text in Research Archive.
    • Tran, Trung; Bollineni, Ravi Chand; Strozynski, Margarita Anna; Koehler, Christian & Thiede, Bernd (2017). Identification of Alternative Splice Variants Using Unique Tryptic Peptide Sequences for Database Searches. Journal of Proteome Research.  ISSN 1535-3893.  16(7), s 2571- 2578 . doi: 10.1021/acs.jproteome.7b00126
    • Arntzen, Magnus Øverlie; Boddie, Paul; Frick, Rahel; Koehler, Christian & Thiede, Bernd (2015). Consolidation of proteomics data in the Cancer proteomics database. Proteomics.  ISSN 1615-9853.  15(22), s 3765- 3771 . doi: 10.1002/pmic.201500144
    • Chymkowitch, Pierre; Nguéa P, Aurélie; Aanes, Håvard; Koehler, Christian; Thiede, Bernd; Lorenz, Susanne; Meza, Leonardo Zepeda; Klungland, Arne & Enserink, Jorrit (2015). Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes. Genome Research.  ISSN 1088-9051.  25(6), s 897- 906 . doi: 10.1101/gr.185793.114
    • Koehler, Christian; Arntzen, Magnus Øverlie & Thiede, Bernd (2015). The impact of carbon-13 and deuterium on relative quantification of proteins using stable isotope diethyl labeling. Rapid Communications in Mass Spectrometry.  ISSN 0951-4198.  29(9), s 830- 836 . doi: 10.1002/rcm.7170
    • Øverbye, Anders; Skotland, Tore; Koehler, Christian; Thiede, Bernd; Seierstad, Therese; Berge, Viktor; Sandvig, Kirsten & Llorente, Alicia (2015). Identification of prostate cancer biomarkers in urinary exosomes. OncoTarget.  ISSN 1949-2553.  6(30), s 30357- 30376 . doi: 10.18632/oncotarget.4851
    • Koehler, Christian; Arntzen, Magnus; De Souza, Gustavo Antonio & Thiede, Bernd (2013). An Approach for Triplex-Isobaric Peptide Termini Labeling (Triplex-IPTL). Analytical Chemistry.  ISSN 0003-2700.  85(4), s 2478- 2485 . doi: 10.1021/ac3035508
    • Størvold, Gro Leite; Landskron, Johannes; Strozynski, Margarita Anna; Arntzen, Magnus; Koehler, Christian; Kalland, Maria Elisabeth; Tasken, Kjetil & Thiede, Bernd (2013). Quantitative profiling of tyrosine phosphorylation revealed changes in the activity of the T cell receptor signaling pathway upon cisplatin-induced apoptosis. Journal of Proteomics.  ISSN 1874-3919.  91, s 344- 357 . doi: 10.1016/j.jprot.2013.07.019
    • Thiede, Bernd; Koehler, Christian; Strozynski, Margarita Anna; Treumann, Achim; Stein, Robert; Zimny-Arndt, Ursula; Schmid, Monika & Jungblut, Peter R. (2013). High Resolution Quantitative Proteomics of HeLa Cells Protein Species Using Stable Isotope Labeling with Amino Acids in Cell Culture(SILAC), Two-Dimensional Gel Electrophoresis(2DE) and Nano-Liquid Chromatograpohy Coupled to an LTQ-OrbitrapMass Spectrometer. Molecular & Cellular Proteomics.  ISSN 1535-9476.  12(2), s 529- 538 . doi: 10.1074/mcp.M112.019372
    • Koehler, Christian; Arntzen, Magnus; Treumann, Achim & Thiede, Bernd (2012). A Rapid Approach for Isobaric Peptide Termini Labeling. Methods in molecular biology.  ISSN 1064-3745.  893, s 129- 141 . doi: 10.1007/978-1-61779-885-6_10
    • Koehler, Christian; Arntzen, Magnus; Treumann, Achim & Thiede, Bernd (2012). Comparison of data analysis parameters and MS/MS fragmentation techniques for quantitative proteome analysis using isobaric peptide termini labeling (IPTL). Analytical and Bioanalytical Chemistry.  ISSN 1618-2642.  404(4), s 1103- 1114 . doi: 10.1007/s00216-012-5949-z
    • Arntzen, Magnus; Koehler, Christian; Barsnes, Harald; Berven, Frode; Treumann, Achim & Thiede, Bernd (2011). IsobariQ: Software for Isobaric Quantitative Proteomics using IPTL, iTRAQ, and TMT. Journal of Proteome Research.  ISSN 1535-3893.  10(2), s 913- 920 . doi: 10.1021/pr1009977
    • Arntzen, Magnus; Koehler, Christian; Treumann, Achim & Thiede, Bernd (2011). Quantitative proteome analysis using isobaric peptide termini labeling (IPTL). Methods in molecular biology.  ISSN 1064-3745.  753, s 65- 76 . doi: 10.1007/978-1-61779-148-2_5
    • Koehler, Christian; Arntzen, Magnus; Strozynski, Margarita Anna; Treumann, Achim & Thiede, Bernd (2011). Isobaric Peptide Termini Labeling Utilizing Site-Specific N-Terminal Succinylation. Analytical Chemistry.  ISSN 0003-2700.  83(12), s 4775- 4781 . doi: 10.1021/ac200229w
    • Kozielski, Frank; Riaz, Tahira; DeBonis, Salvatore; Koehler, Christian; Panse, Isabel; Strozynski, Margarita Anna; Donaldson, Ian Matthew; Thiede, Bernd & Kroening, Mario (2011). Proteome analysis of microtubule-associated proteins and their interacting partners from mammalian brain. Amino Acids.  ISSN 0939-4451.  41(2), s 363- 385 . doi: 10.1007/s00726-010-0649-5
    • Schmidt, Frank; Dahlmann, Burkhardt; Hustoft, Hanne Kolsrud; Koehler, Christian; Strozynski, Margarita Anna; Kloss, Alexander; Zimny-Arndt, Ursula; Jungblut, Peter R. & Thiede, Bernd (2011). Quantitative proteome analysis of the 20S proteasome of apoptotic Jurkat T cells. Amino Acids.  ISSN 0939-4451.  41(2), s 351- 361 . doi: 10.1007/s00726-010-0575-6
    • Dørum, Siri; Arntzen, Magnus; Qiao, Shuo Wang; Holm, Anders; Koehler, Christian; Thiede, Bernd; Sollid, Ludvig Magne & Fleckenstein, Burkhard (2010). The Preferred Substrates for Transglutaminase 2 in a Complex Wheat Gluten Digest Are Peptide Fragments Harboring Celiac Disease T-Cell Epitopes. PLoS ONE.  ISSN 1932-6203.  5(11) . doi: 10.1371/journal.pone.0014056 Show summary
    • Kozielski, Frank; Riaz, Tahira; DeBonis, Salvatore; Koehler, Christian; Kröning, Mario; Panse, Isabel; Strozynski, Margarita Anna; Donaldson, Ian M & Thiede, Bernd (2010). Proteome analysis of microtubule-associated proteins and their interacting partners from mammalian brain. Amino Acids.  ISSN 0939-4451. . doi: 10.1007/s00726-010-0649-5
    • De Souza, Gustavo; Fortuin, Suereta; Aguilar, Diana; Pando, Rogelio Hernandez; McEvoy, Christopher R.E.; van Helden, Paul D.; Koehler, Christian J; Thiede, Bernd; Warren, Robin M & Wiker, Harald G (2010). Using a Label-free Proteomics Method to Identify Differentially Abundant Proteins in Closely Related Hypo- and Hypervirulent Clinical Mycobacterium tuberculosis Beijing Isolates. Molecular & Cellular Proteomics.  ISSN 1535-9476.  9(11), s 2414- 2423 . doi: 10.1074/mcp.M900422-MCP200 Show summary
    • Saunders, Threrese Solstad; Bjørgo, Elisa; Koehler, Christian; Strozynski, Margarita Anna; Torgersen, Knut Martin; Tasken, Kjetil & Thiede, Bernd (2010). Quantitative proteome analysis of detergent-resistant membranes identifies the differential regulation of protein kinase C isoforms in apoptotic T cells. Proteomics.  ISSN 1615-9853.  10(15), s 2758- 2768 . doi: 10.1002/pmic.201000164
    • Schmidt, Frank; Dahlmann, Burkhardt; Hustoft, Hanne Kolsrud; Koehler, Christian; Strozynski, Margarita Anna; Klof, Alexander; Zimmy-Arndt, Ursula; Jungblut, Peter R. & Thiede, Bernd (2010). Quantitative proteome analysis of the 20S proteasome of apoptotic Jurkat T cells. Amino Acids.  ISSN 0939-4451. . doi: 10.1007/s00726-010-0575-6
    • Tomazella, Gisele Guiqardi; daSilva, Idalete; Thomé, Carolina H.; Greene, Lewis J; Koehler, Christian J; Thiede, Bernd; Wiker, Harald G & De Souza, Gustavo (2010). Analysis of detergent-insoluble and whole cell lysate fractions of resting neutrophils using high-resolution mass spectrometry. Journal of Proteome Research.  ISSN 1535-3893.  9(4), s 2030- 2036 . doi: 10.1021/pr1000253 Show summary
    • de Souza, GA; De Souza, Gustavo; Søfteland, Tina; Softeland, T; Koehler, Christian J; Thiede, Bernd & Wiker, Harald G (2009). Validating divergent ORF annotation of the Mycobacterium leprae genome through a full translation dataset and peptide identification by tandem mass spectrometry. Proteomics.  ISSN 1615-9853.  9(12), s 3233- 3243 . doi: 10.1002/pmic.200800955 Show summary
    • Koehler, Christian; Strozynski, Margarita Anna; Kozielski, F; Treumann, A & Thiede, Bernd (2009). Isobaric Peptide Termini Labeling for MS/MS-Based Quantitative Proteomics. Journal of Proteome Research.  ISSN 1535-3893.  8(9), s 4333- 4341 . doi: 10.1021/pr900425n

    View all works in Cristin

    • Bollineni, Ravi Chand; Koehler, Christian; Gislefoss, Randi Elin; Anonsen, Jan Haug & Thiede, Bernd (2018). Author Correction: Large-scale intact glycopeptide identification by Mascot database search. Scientific Reports.  ISSN 2045-2322.  8 . doi: 10.1038/s41598-018-28147-w
    • Thiede, Bernd; Koehler, Christian; Strozynski, Margarita Anna; Treumann, A; Stein, Robert; Zimny-Amdt, Ursula; Schmid, Monika & Jungblut, Peter R. (2012). Protein species high resolution quantitative proteomics of HeLa cells using SILAC-2-DE-nanoLC/LTQ-Orbitrap mass spectrometry. Molecular & Cellular Proteomics.  ISSN 1535-9476. . doi: 10.1074/mcp.M112.019372
    • Arntzen, Magnus; Koehler, Christian; Barsnes, Harald; Berven, Frode; Treumann, Achim & Thiede, Bernd (2011). IsobariQ: Software for isobaric quantitative proteomics using IPTL, iTRAQ and TMT.
    • De Souza, Gustavo; Fortuin, Suereta; McEvoy, Christopher R; Pando, Rogelio Hernandez; Koehler, Christian J; Thiede, Bernd; Warren, Robin M & Wiker, Harald G (2009). A label-free method identified differentially abundant proteins in related M.tuberculosis Beijing strains.

    View all works in Cristin

    Published Mar. 1, 2016 10:05 AM - Last modified Mar. 1, 2016 2:48 PM