Frida Forsberg

• Brunet, Annaëlle; Forsberg, Anna Frida; Fan, Qiong; Sæther, Thomas & Collas, Philippe (2019). Nuclear lamin B1 interactions with chromatin during the circadian cycle are uncoupled from periodic gene expression. Frontiers in Genetics.  ISSN 1664-8021.  10 . doi: 10.3389/fgene.2019.00917 Full text in Research Archive. Show summary

  Many mammalian genes exhibit circadian expression patterns concordant with periodic binding of transcription factors, chromatin modifications, and chromosomal interactions. Here we investigate whether chromatin periodically associates with nuclear lamins. Entrainment of the circadian clock is accompanied, in mouse liver, by a net gain of lamin B1–chromatin interactions genome-wide, after which the majority of lamina-associated domains (LADs) are conserved during the circadian cycle. By tailoring a bioinformatics pipeline designed to identify periodic gene expression patterns, we also observe hundreds of variable lamin B1-chromatin interactions among which oscillations occur at 64 LADs, affecting one or both LAD extremities or entire LADs. Only a small subset of these oscillations however exhibit highly significant 12, 18, 24, or 30 h periodicity. These periodic LADs display oscillation asynchrony between their 5′ and 3′ borders, and are uncoupled from periodic gene expression within or in the vicinity of these LADs. Periodic gene expression is also unrelated to variations in gene-to-nearest LAD distances detected during the circadian cycle. Accordingly, periodic genes, including central clock-control genes, are located megabases away from LADs throughout circadian time, suggesting stable residence in a transcriptionally permissive chromatin environment. We conclude that periodic LADs are not a dominant feature of variable lamin B1–chromatin interactions during the circadian cycle in mouse liver. Our results also suggest that periodic hepatic gene expression is not regulated by rhythmic chromatin associations with the nuclear lamina.

• Forsberg, Frida; Brunet, Annaëlle; Liyakat Ali, Tharvesh Moideen & Collas, Philippe (2019). Interplay of lamin A and lamin B LADs on the radial positioning of chromatin. Nucleus.  ISSN 1949-1034.  10(1), s 7- 20 . doi: 10.1080/19491034.2019.1570810 Full text in Research Archive.

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