Ine-Susanne Hopland Methlie

• Kauserud, Håvard; Justad, Tor Arne; Vindenes, Yngvild; Methlie, Ine-Susanne Hopland; Sønstebø, Jørn Henrik & Skrede, Inger [Show all 7 contributors for this article] (2024). Limited evidence of local adaptation of growth and decomposition rates in the widespread wood-decay fungus Fomitopsis pinicola. Fungal ecology. ISSN 1754-5048. 70. doi: 10.1016/j.funeco.2024.101353.
• Peris, David; Lü, Dabao Sun; Kinneberg, Vilde Bruhn; Methlie, Ine-Susanne Hopland; Stapnes Dahl, Malin & Y. James, Timothy [Show all 8 contributors for this article] (2022). Large-scale fungal strain sequencing unravels the molecular diversity in mating loci maintained by long-term balancing selection. PLoS Genetics. ISSN 1553-7390. 18(3). doi: 10.1371/journal.pgen.1010097. Full text in Research Archive Show summary

  Balancing selection, an evolutionary force that retains genetic diversity, has been detected in multiple genes and organisms, such as the sexual mating loci in fungi. However, to quantify the strength of balancing selection and define the mating-related genes require a large number of strains. In tetrapolar basidiomycete fungi, sexual type is determined by two unlinked loci, MATA and MATB. Genes in both loci define mating type identity, control successful mating and completion of the life cycle. These loci are usually highly diverse. Previous studies have speculated, based on culture crosses, that species of the non-model genus Trichaptum (Hymenochaetales, Basidiomycota) possess a tetrapolar mating system, with multiple alleles. Here, we sequenced a hundred and eighty strains of three Trichaptum species. We characterized the chromosomal location of MATA and MATB, the molecular structure of MAT regions and their allelic richness. The sequencing effort was sufficient to molecularly characterize multiple MAT alleles segregating before the speciation event of Trichaptum species. Analyses suggested that long-term balancing selection has generated trans-species polymorphisms. Mating sequences were classified in different allelic classes based on an amino acid identity (AAI) threshold supported by phylogenetics. 17,550 mating types were predicted based on the allelic classes. In vitro crosses allowed us to support the degree of allelic divergence needed for successful mating. Even with the high amount of divergence, key amino acids in functional domains are conserved. We conclude that the genetic diversity of mating loci in Trichaptum is due to long-term balancing selection, with limited recombination and duplication activity. The large number of sequenced strains highlighted the importance of sequencing multiple individuals from different species to detect the mating-related genes, the mechanisms generating diversity and the evolutionary forces maintaining them.

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• Lü, Dabao Sun; Methlie, Ine-Susanne Hopland; Peris Navarro, David; Kauserud, Håvard & Skrede, Inger (2022). Postglacial history of a widespread forest fungus in Europe: Migration out of multiple refugia followed by secondary admixture .
• Peris Navarro, David; Lü, Dabao Sun; Kinneberg, Vilde Bruhn; Methlie, Ine-Susanne Hopland; James, Timothy & Kauserud, Håvard [Show all 7 contributors for this article] (2022). Ancient mating alleles maintained by balancing selection in basidiomycete fungi.
• Birkemoe, Tone; Skrede, Inger & Methlie, Ine-Susanne Hopland (2022). Mindre mat for kresne biller. https://forskning.no/.
• Peris, David; Lü, Dabao Sun; Kinneberg, Vilde Bruhn; Methlie, Ine-Susanne Hopland; Dahl, Malin Stapnes & James, Timothy Y. [Show all 8 contributors for this article] (2022). Long-term balancing selection in a white-root fungi.
• Peris, David; Lü, Dabao Sun; Kinneberg, Vilde Bruhn; Methlie, Ine-Susanne Hopland; Dahl, Malin Stapnes & James, Timothy Y. [Show all 8 contributors for this article] (2021). Northern hemisphere fungal specimens unravel dynamic and polymorphic nature of mating loci.

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