Leica TIRF, room 3604

This microscope is set up for live cell imaging and uses the property of total internal reflection to illuminate fluorophores within the evanascent field of the sample.

Imaging platform

Photo: UiO

Information about the microscope

Total Internal Reflection Fluorescence (TIRF) microscopes llows efficient detection of fluorescence within regions close to the sample/glass interface with a high sensitivity, which minimizes the damage to living cells. The system offers three wavelengths: 488nm, 561nm, and 635nm; with fast AOTF control. Image acquisition speed can be increased to several images per second using the quad cube filter to avoid repeated mechanical filter changes. Various TIRF slice thicknesses are possible, depending on the excitation wavelength, as well as epifluorescence imaging.

 

Specifications:

  •     Heating chamber
  •     CO2 controller
  •     Motorized stage
  •     Autofocus
  •     Peristaltic perfusion chamber

Laser lines:

  •     3 solid state lasers:
    •         488 nm
    •         561 nm
    •         635 nm 

Objectives:

Magnification Type Numerical Aperture Working distance(mm) Immersion
20x N Plan 0.4 0.39 -
40x HCX PlanApo 1.25 0.1 Oil
63x HCX PlanApo 1.47 0.1 Oil
100x HCX PlanApo 1.47 0.1 Oil

 

 

 

 

 

 

 

 

 

 

More information from Leica on the TIRF system and objectives.

    Published Dec. 2, 2020 8:52 AM - Last modified Mar. 2, 2021 5:45 PM