Hanne Røberg-Larsen

• Uhlig, Silvio; Cai, Kun; Liskiewicz, Krystyna Anna; Pain, Maria; Aiko Grutle, Lene & Røberg-Larsen, Hanne [Show all 7 contributors for this article] (2024). Profiling of cyclic di-adenyl and -guanyl nucleotides and their precursors and degradation products in bacteria using LC–MS/MS. Separation Science Plus (SSC plus). ISSN 2573-1815. doi: 10.1002/sscp.202400039.
• Hrušková, Helena; Olsen, Christine; Řemínek, Roman; Wang, Chencheng; Aizenshtadt, Aleksandra & Krauss, Stefan Johannes Karl [Show all 10 contributors for this article] (2024). Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. Journal of Chromatography A. ISSN 0021-9673. 1717. doi: 10.1016/j.chroma.2024.464669.
• Aizenshtadt, Aleksandra; Wang, Chencheng; Abadpour, Shadab; Menezes, Pedro Duarte; Wilhelmsen, Ingrid & Dalmao-Fernandez, Andrea [Show all 15 contributors for this article] (2024). Pump-Less, Recirculating Organ-on-Chip (rOoC) Platform to Model the Metabolic Crosstalk between Islets and Liver. Advanced Healthcare Materials. ISSN 2192-2640. doi: 10.1002/adhm.202303785.
• Kømurcu, Kristina Sæterdal; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2024). LC-MS Approaches for Oxysterols in Various Biosamples. Advances in Experimental Medicine and Biology. ISSN 0065-2598. 1440, p. 57–71. doi: 10.1007/978-3-031-43883-7_4.
• Kømurcu, Kristina Sæterdal; Wilhelmsen, Ingrid; Thorne, James L.; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon & Aizenshtadt, Aleksandra [Show all 7 contributors for this article] (2023). Mass spectrometry reveals that oxysterols are secreted from non-alcoholic fatty liver disease induced organoids. Journal of Steroid Biochemistry and Molecular Biology. ISSN 0960-0760. 232. doi: 10.1016/j.jsbmb.2023.106355. Full text in Research Archive
• Kogler, Stian; Kømurcu, Kristina Sæterdal; Olsen, Christine Sandsnes; Shoji, Jun-ya; Skottvoll, Frøydis Sved & Krauss, Stefan Johannes Karl [Show all 8 contributors for this article] (2023). Organoids, organ-on-a-chip, separation science and mass spectrometry: An update. TrAC. Trends in analytical chemistry. ISSN 0165-9936. 161. doi: 10.1016/j.trac.2023.116996. Full text in Research Archive
• Kogler, Stian; Aizenshtadt, Aleksandra; Harrison, Sean; Skottvoll, Frøydis Sved; Berg, Henriette Engen & Abadpour, Shadab [Show all 14 contributors for this article] (2022). "Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry. Analytical Chemistry. ISSN 0003-2700. 94(50), p. 17677–17684. doi: 10.1021/acs.analchem.2c04530. Full text in Research Archive
• Røberg-Larsen, Hanne; Lundanes, Elsa; Nyman, Tuula Anneli; Berven, Frode Steingrimsen & Wilson, Steven Ray Haakon (2021). Liquid chromatography, a key tool for the advancement of single-cell omics analysis. Analytica Chimica Acta. ISSN 0003-2670. 1178:338551, p. 1–9. doi: 10.1016/j.aca.2021.338551.
• Websdale, Alex; Kiew, Yi; Chalmers, Philip; Chen, Xinyu; Cioccoloni, Giorgia & Hughes, Thomas A. [Show all 14 contributors for this article] (2021). Pharmacologic and genetic inhibition of cholesterol esterification enzymes reduces tumour burden: A systematic review and meta-analysis of preclinical models. Biochemical Pharmacology. ISSN 0006-2952. doi: 10.1016/j.bcp.2021.114731.
• Hutchinson, Samantha A.; Websdale, Alex; Cioccoloni, Giorgia; Røberg-Larsen, Hanne; Lianto, Priscilia & Kim, Baek [Show all 18 contributors for this article] (2021). Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancer. Oncogene. ISSN 0950-9232. 40(16), p. 2872–2883. doi: 10.1038/s41388-021-01720-w. Full text in Research Archive
• Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon & Lundanes, Elsa (2021). Recent advances in on-line upfront devices for sensitive bioanalytical nano LC methods. TrAC. Trends in analytical chemistry. ISSN 0165-9936. 136, p. 1–10. doi: 10.1016/j.trac.2021.116190.
• Tsjokajev, Ahmad; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon; Anne Berit Dyve, Lingelem; Skotland, Tore & Sandvig, Kirsten [Show all 7 contributors for this article] (2020). Mass spectrometry-based measurements of cyclic adenosine monophosphate in cells, simplified using reversed phase liquid chromatography with a polar characterized stationary phase. Journal of chromatography. B. ISSN 1570-0232. 1160, p. 1–6. doi: 10.1016/j.jchromb.2020.122384. Full text in Research Archive
• Becares, Natalia; Cage, Matthew C; Voisin, Maud; Shrestha, Elina; Martin-Gutierrez, Lucia & Liang, Ning [Show all 20 contributors for this article] (2019). Impaired LXRα Phosphorylation Attenuates Progression of Fatty Liver Disease. Cell reports. ISSN 2211-1247. 26(4), p. 984–995.e6. doi: 10.1016/j.celrep.2018.12.094. Full text in Research Archive
• Solheim, Stian; Hutchinson, Samantha A; Lundanes, Elsa; Wilson, Steven Ray Haakon; Thorne, James L & Røberg-Larsen, Hanne (2019). Fast liquid chromatography-mass spectrometry reveals side chain oxysterol heterogeneity in breast cancer tumour samples. Journal of Steroid Biochemistry and Molecular Biology. ISSN 0960-0760. 192. doi: 10.1016/j.jsbmb.2019.02.004. Full text in Research Archive
• Hutchinson, Samantha A; Lianto, Priscilia; Røberg-Larsen, Hanne; Battaglia, Sebastiano; Hughes, Thomas A & Thorne, James L (2019). ER-Negative Breast Cancer Is Highly Responsive to Cholesterol Metabolite Signalling. Nutrients. ISSN 2072-6643. 11(11), p. 1–18. doi: 10.3390/nu11112618. Full text in Research Archive
• Skottvoll, Frøydis Sved; Berg, Henriette Engen; Bjørseth, Kamilla; Lund, Kaja; Roos, Norbert & Bekhradnia, Sara [Show all 13 contributors for this article] (2018). Ultracentrifugation versus kit exosome isolation: nanoLC-MS and other tools reveal similar performance biomarkers, but also contaminations. Future science OA. ISSN 2056-5623. 5(1). doi: 10.4155/fsoa-2018-0088. Full text in Research Archive
• Dias, Irundika H.K.; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2018). Chromatography of oxysterols. Biochimie. ISSN 0300-9084. doi: 10.1016/j.biochi.2018.05.004.
• Røberg-Larsen, Hanne; Abele, Silvija; Demir, Deniz; Dzabijeva, Diana; Amundsen, Sunniva Furre & Wilson, Steven Ray Haakon [Show all 8 contributors for this article] (2017). Rugged Large Volume Injection for Sensitive Capillary LC-MS Environmental Monitoring. Frontiers in Chemistry. ISSN 2296-2646. AUG(2017). doi: 10.3389/fchem.2017.00062. Full text in Research Archive
• Gregus, Michael; Røberg-Larsen, Hanne; Lundanes, Elsa; Foret, Frantisek; Kubán, Petr & Wilson, Steven Ray Haakon (2017). Non-aqueous capillary electrophoretic separation of cholesterol and 25-hydroxycholesterol after derivatization with Girard P reagent. Chemistry and Physics of Lipids. ISSN 0009-3084. 207, p. 87–91. doi: 10.1016/j.chemphyslip.2017.05.014.
• Røberg-Larsen, Hanne; Lund, Kaja; Sæterdal, Kristina Erikstad; Solheim, Stian; Vehus, Tore & Solberg, Nina [Show all 9 contributors for this article] (2017). Mass spectrometric detection of 27-hydroxycholesterol in breast cancer exosomes. Journal of Steroid Biochemistry and Molecular Biology. ISSN 0960-0760. 169, p. 22–28. doi: 10.1016/j.jsbmb.2016.02.006.
• Åstrand, Ove Alexander Høgmoen; Viktorsson, Elvar Örn; Kristensen, Aleksander Lim; Ekeberg, Dag; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon [Show all 12 contributors for this article] (2017). Synthesis, in vitro and in vivo biological evaluation of new oxysterols as modulators of the liver X receptors. Journal of Steroid Biochemistry and Molecular Biology. ISSN 0960-0760. 165, p. 323–330. doi: 10.1016/j.jsbmb.2016.07.010. Full text in Research Archive
• Vehus, Tore; Røberg-Larsen, Hanne; Waaler, Jo; Aslaksen, Sigrid; Krauss, Stefan & Wilson, Steven Ray Haakon [Show all 7 contributors for this article] (2016). Versatile, sensitive liquid chromatography mass spectrometry–Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins. Scientific Reports. ISSN 2045-2322. 6. doi: 10.1038/srep37507. Full text in Research Archive
• Simigdala, Nikiana; Gao, Qiong; Pancholi, Sunil; Røberg-Larsen, Hanne; Zvelebil, Marketa & Ribas, Ricardo [Show all 11 contributors for this article] (2016). Cholesterol biosynthesis pathway as a novel mechanism of resistance to estrogen deprivation in estrogen receptor-positive breast cancer. Breast Cancer Research. ISSN 1465-5411. 18:58. doi: 10.1186/s13058-016-0713-5.
• Brandtzaeg, Ole Kristian; Johnsen, Elin Follaug; Røberg-Larsen, Hanne; Seip, Knut Fredrik; MacLean, Evan L. & Gesquiere, Laurence R. [Show all 9 contributors for this article] (2016). Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum. Scientific Reports. ISSN 2045-2322. 6:31693. doi: 10.1038/srep31693. Full text in Research Archive
• Johnsen, Elin Follaug; Brandtzaeg, Ole Kristian; Vehus, Tore; Røberg-Larsen, Hanne; Bogoeva, Vanya & Ademi, Ornela [Show all 9 contributors for this article] (2016). A critical evaluation of Amicon Ultra centrifugal filters for separating proteins, drugs and nanoparticles in biosamples. Journal of Pharmaceutical and Biomedical Analysis. ISSN 0731-7085. 120, p. 106–111. doi: 10.1016/j.jpba.2015.12.010.
• Røberg-Larsen, Hanne; Vesterdal, Caroline; Wilson, Steven Ray Haakon & Lundanes, Elsa (2015). Underivatized oxysterols and nanoLC-ESI-MS: A mismatch. Steroids. ISSN 0039-128X. 99, p. 125–130. doi: 10.1016/j.steroids.2015.01.023.
• Røberg-Larsen, Hanne; Strand, Martin Frank; Krauss, Stefan & Wilson, Steven Ray Haakon (2014). Metabolites in vertebrate Hedgehog signaling. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 446(3), p. 669–674. doi: 10.1016/j.bbrc.2014.01.087.
• Røberg-Larsen, Hanne; Lund, Kaja; Vehus, Tore; Solberg, Nina; Vesterdal, Caroline & Misaghian, Dorna [Show all 10 contributors for this article] (2014). Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols. Journal of Lipid Research. ISSN 0022-2275. 55(7), p. 1531–1536. doi: 10.1194/jlr.D048801.
• Røgeberg, Magnus; Malerød, Helle; Røberg-Larsen, Hanne; Aass, Cecilie & Wilson, Steven Ray Haakon (2013). On-line solid phase extraction-liquid chromatography, with emphasis on modern bioanalysis and miniaturized systems. Journal of Pharmaceutical and Biomedical Analysis. ISSN 0731-7085. 87(18), p. 120–129. doi: 10.1016/j.jpba.2013.05.006.
• Kalluru, Raja Sab; Fenaroli, Federico; Westmoreland, David; Ulanova, Lilia; Maleki, Atoosa & Roos, Norbert [Show all 15 contributors for this article] (2013). Polylactide-co-glycolide-rifampicin-nanoparticles efficiently clear Mycobacterium bovis BCG infection in macrophages and remain membrane-bound in phago-lysosomes. Journal of Cell Science. ISSN 0021-9533. 126(14), p. 3043–3054. doi: 10.1242/jcs.121814.
• Røberg-Larsen, Hanne; Strand, Martin Frank; Grimsmo, Anders; Olsen, Peter Angell; Dembinski, Jennifer & Rise, Frode [Show all 10 contributors for this article] (2012). High sensitivity measurements of active oxysterols with automated filtration/filter backflush-solid phase extraction-liquid chromatography-mass spectrometry. Journal of Chromatography A. ISSN 0021-9673. 1255, p. 291–297. doi: 10.1016/j.chroma.2012.02.002.
• Svendsen, Kristin Opsal; Røberg-Larsen, Hanne; Pedersen, Siri A.; Brenna, Ida; Lundanes, Elsa & Wilson, Steven Ray Haakon (2011). Automatic filtration and filter flush for robust online solid-phase extraction liquid chromatography. Journal of Separation Science. ISSN 1615-9306. 34(21), p. 3020–3022. doi: 10.1002/jssc.201100553.

View all works in Cristin

• Kogler, Stian; Aizenshtadt, Aleksandra; Harrison, Sean Philip; Sullivan, Gareth John; Lundanes, Elsa & Bogen, Inger Lise [Show all 9 contributors for this article] (2023). Organ-in-a-column – automated on-line incubation, sampling, and LC-MS analysis for measurement of drug metabolites.
• Røberg-Larsen, Hanne; Kømurcu, Kristina Sæterdal; Kogler, Stian; Olsen, Christine; Skottvoll, Frøydis Sved & Aizenshtadt, Aleksandra [Show all 8 contributors for this article] (2023). Organoids, Organ-on-a-chip and Mass Spectrometry.
• Midtøy, Lise; Kømurcu, Kristina Sæterdal; Kogler, Stian; wilhelmsen, ingrid; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2023). Sensitive targeted proteomic analysis of cholesterol metabolism enzymes in hepatic stellate cells - going towards single cell analysis .
• kvalvik, Eva; Kogler, Stian; Kømurcu, Kristina Sæterdal; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2023). Screening for biomarkers for non-alcoholic fatty liver disease using organ-in-a-column technology.
• Bull Tuner, Marius; Kogler, Stian; Hansen, Frederik Andre; Pedersen-Bjergaard, Stig; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2023). Exploring electromembrane extraction and LC-MS for studying liver organoid metabolomics.
• Haavaag, Jon Erlend; Kogler, Stian; Zawadzka, Malgorzata Elzbieta; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2023). Building a high-resolution nano DESI-MS for organoid imaging: Analysis using a built and optimized imaging system.
• Mathingsdal Pedersen, Gustav; Kogler, Stian & Røberg-Larsen, Hanne (2023). On-line Sample Preparation for "Organ-in-a-Column".
• Skottvoll, Frøydis Sved; Aizenshtadt, Aleksandra; Harrison, Sean; Krauss, Stefan Johannes Karl; Sullivan, Gareth & Bogen, Inger Lise [Show all 9 contributors for this article] (2022). Organ-in-a-column - On-line incubation and LC-MS-analysis for drug metabolism studies.
• Wilson, Steven Ray Haakon; Røberg-Larsen, Hanne; Krauss, Stefan Johannes Karl & Aizenshtadt, Aleksandra (2022). Organ-in-a-column - An automated on-line incubation and analysis system for drug metabolism studies.
• Kømurcu, Kristina Sæterdal; Aizenshtadt, Aleksandra; Thorne, James L.; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2022). Oxysterols are secreted from Non-Alcoholic Fatty Liver Disease (NAFLD) induced organoids.
• Røberg-Larsen, Hanne (2022). On-line prøveopparbeidelse – Hva er det og hvorfor trenger vi det?
• midtøy, Lise; Kømurcu, Kristina Sæterdal; Kogler, Stian; wilhelmsen, Ingrid; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2022). Sensitive targeted proteomic analysis of cholesterol metabolism enzymes in hepatic stellate cells - going towards single cell analysis .
• Bull Tuner, Marius; Kogler, Stian; Hansen, Frederik Andre; Pedersen-Bjergaard, Stig; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2022). Exploring electromembrane extraction for sampling the organoid metabolome.
• Kømurcu, Kristina Sæterdal; Aizenshtadt, Aleksandra; Thorne, James L; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2022). Measuring oxysterols secreted from liver organoids by LC-MS for the understanding of Non-alcoholic fatty liver disease .
• Kømurcu, Kristina Sæterdal; Aizenshtadt, Aleksandra; Thorne, James L; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2022). Oxysterols are secreted from Non-alcoholic Fatty Liver disease induced organoids.
• Kømurcu, Kristina Sæterdal; Aizenshtadt, Aleksandra; Thorne, James L; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2022). Measuring oxysterols secreted from liver organoids by LC-MS for the understanding of Non-alcoholic fatty liver disease.
• Kømurcu, Kristina Sæterdal; Aizenshtadt, Aleksandra; Thorne, James L; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2022). Measuring oxysterols using LC-MS for the understanding of non alcoholic fatty liver disease (NAFLD).
• Kogler, Stian; Wilson, Steven Ray Haakon; Røberg-Larsen, Hanne; Krauss, Stefan Johannes Karl; Lundanes, Elsa & Skottvoll, Frøydis Sved (2021). Organ-in-a-column.
• Moe, Harald; Lundanes, Elsa; Wilson, Steven Ray Haakon; Lund, Reidar & Røberg-Larsen, Hanne (2021). Releasing anitmicrobial peptides through liposomal nanocarriers to fight multidrug-resistant bacteria.
• Nordli, Henriette; Røberg-Larsen, Hanne; Lundanes, Elsa & Wilson, Steven Ray Haakon (2021). Determination of sterols in liver organoids using liquid chromatography-mass spectrometry (LC-MS).
• Nordli, Henriette; Aizenshtadt, Aleksandra; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2021). Oxysterols secreted from a Non-Alcoholic Fatty Liver Disease (NAFLD) induced liver organoids.
• Websdale, Alex; Hutchinson, S.A.; Lianto, P.; Røberg-Larsen, Hanne; Wastall, L.M. & Williams, B. [Show all 10 contributors for this article] (2020). Cholesterol side-chain hydroxylation is associated with expression of P-glycoprotein and disease-free survival in triple negative breast cancer patients. Proceedings of the Nutrition Society. ISSN 0029-6651. p. 1–1. doi: 10.1017/S0029665120007429.
• Røberg-Larsen, Hanne; Websdale, Alex; Solheim, Stian Kjønnås; Sande, Maria Therese; Hutchinson, Samantha A & Lundanes, Elsa [Show all 8 contributors for this article] (2020). Fast LC-MS for determination of oxysterols in breast cancer tumours.
• Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2020). Low-flow Rate Separations of Lipids. In Griffiths, William & Wang, Yuqing (Ed.), Lipidomics: Current and Emerging Techniques . Royal Society of Chemistry (RSC) Publishing. ISSN 978-1-78801-160-0. p. 49–73. doi: 10.1039/9781788013109-00049.
• Wilson, Steven Ray Haakon; Berg, Henriette Engen; Røberg-Larsen, Hanne & Lundanes, Elsa (2019). Hyphenations of one-dimensional capillary liquid chromatography with mass spectrometry: state-of-the-art applications . In Tranchida, Peter & Mondello, Luigi (Ed.), Hyphenations of Capillary Chromatography with Mass Spectrometry. Elsevier. ISSN 9780128096383. p. 319–358.
• Berg, Henriette Engen; Skottvoll, Frøydis Sved; Bjørseth, Kamilla; Sæterdal, Kristina Erikstad; Brandtzaeg, Ole Kristian & Vehus, Tore [Show all 11 contributors for this article] (2018). Development of nanoLC column technology for proteomic studies . Show summary

  Background NanoLC has emerged as a major technique in proteomics due to improved sensitivity, which enables analysis of small sample volumes and low concentrations. In our group (Bioanalytical chemistry, BACH) we focus on the development of column technology, including porous layer open tubular (PLOT) columns and self-packing of columns for proteomic applications. Methods The PLOT columns were used for both on-line digestion (enzyme reactors) and separations (10 µm ID, PS-DVB with ODS). Enzyme reactors (20 µm ID and multi-PLOT columns (~8 µm ID)) were immobilized with trypsin and Lys-C for protein digestion. We also compared self-packed columns of 50 µm ID (2.6 µm C18 core-shell particles) to a commercial column (75 µm ID). The columns were used for detection of CYP27a1 in MDA-MB-231 (breast cancer) cells, and biomarker candidates in MDA-MB-231 and glioblastoma multiforme (brain cancer) exosomes. For exosome isolation (cell culture medium), ultracentrifugation (“golden standard”) was compared to a Total Exosome Isolation Reagent from Thermo Fisher regarding protein yield and purity. Results Our PLOT columns were successfully employed for proteins, metabolites and peptides with attogram detection limits and peak capacities around 250 [1]. The enzyme reactors provided sufficient digestion in ~5 minutes compared to overnight digestions (common in off-line protocols) [2]. Furthermore, a quantitative method of the neurotoxin ricin was developed using the multi-PLOT to reduce manual sample handling [3] . The self-packed columns obtained peak capacities comparable to the commercial nanoLC column for peptide separations [4]. The self-packed columns were used for the targeted detection of CYP27A1 (potential breast cancer biomarker) in MDA-MB-231 cells. In the recent exosome study, exosomes were present and biomarker candidates were identified. However, the characterization techniques are in our hands not satisfactory. Conclusions We have successfully developed sensitive nanoLC column formats for proteomic applications.

• Røberg-Larsen, Hanne; Sande, Maria Therese; Hutchinson, Samantha A; Solheim, Stian Kjønnås; Lundanes, Elsa & Thorne, James L [Show all 7 contributors for this article] (2018). Rapid determination of oxysterols in breast cancer tumors.
• Brandtzaeg, Ole Kristian; Levernæs, Maren Christin Stillesby; Halvorsen, Trine Grønhaug; Reubsaet, Leon; Røen, Bent Tore & Vehus, Tore [Show all 9 contributors for this article] (2018). Online sample processing systems for targeted nano liquid chromatography mass spectrometry bioanalysis.
• Kular, Ramneet Kaur; Wilson, Steven Ray Haakon; Røberg-Larsen, Hanne & Lundanes, Elsa (2018). Towards ultrafast liquid chromatography of 3’-phosphoadenosine-5’-phosphosulfate using a short hydrophilic interaction chromatography column.
• Røberg-Larsen, Hanne (2018). Prøvefiltrering - offline eller online?
• Røberg-Larsen, Hanne; Hutchinson, Sam Alex; Solheim, Stian; Wilson, Steven Ray Haakon; Lundanes, Elsa & Thorne, James L (2018). Oxysterols in breast cancer.
• Røberg-Larsen, Hanne; Horne, Hildegunn; Holme, Ane Moe; Holm, Maia Blomhoff; Roland, Marie Cecilie & Michelsen, Trond [Show all 8 contributors for this article] (2017). Human in vivo studies of placenta physiology: oxysterols and metabolomic profiling.
• Røberg-Larsen, Hanne; Skottvoll, Frøydis Sved; Solheim, Stian; Bjørseth, Kamilla; Thorne, James L & Lundanes, Elsa [Show all 7 contributors for this article] (2017). Oxysterols in cancer.
• Solheim, Stian; Røberg-Larsen, Hanne; Lundanes, Elsa & Wilson, Steven Ray Haakon (2017). Optimizing selectivity and sensitivity for oxysterol measurements using liquid chromatography-mass spectrometry (LC-MS).
• Sved Skottvoll, Frøydis; Lund, Kaja; Røberg-Larsen, Hanne; Berg, Henriette Sjånes; Thiede, Bernd & Lundanes, Elsa [Show all 7 contributors for this article] (2017). Diagnostic potential of exosomes: The purification issue.
• Demir, Deniz; Abele, Silvija; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon & Lundanes, Elsa (2017). Development of an ultrasensitive method for multiresidue screening of pharmaceutical products in environmental water samples.
• Røberg-Larsen, Hanne; Lund, Kaja; Sved Skottvoll, Frøydis; Gregus, Michael; Lundanes, Elsa & Wilson, Steven Ray Haakon (2016). 27-hydroxycholesterol in cancer exosomes – potential new separation method?
• Wilson, Steven Ray Haakon; Vehus, Tore; Røberg-Larsen, Hanne & Lundanes, Elsa (2016). “Sub-chip” liquid chromatography-mass spectrometry for measuring oxysterols and related proteins.
• Vehus, Tore; Lundanes, Elsa; Røberg-Larsen, Hanne; Brandtzaeg, Ole Kristian & Wilson, Steven Ray Haakon (2016). Open tubular liquid chromatography-mass spectrometry: a powerful and versatile tool for bioanalysis.
• Abele, Silvija; Røberg-Larsen, Hanne; Dzabijeva, Diana; Demir, Deniz; Amundsen, Sunniva F. & Wilson, Steven Ray Haakon [Show all 8 contributors for this article] (2016). Simultaneous determination of pharmaceutical product residues in water by HPLC-MS/MS using online SPE.
• Wilson, Steven Ray Haakon; Lundanes, Elsa; Vehus, Tore & Røberg-Larsen, Hanne (2016). On-line sample preparation and liquid chromatography using "sub-chip" columns for ultra-sensitive proteomics.
• Brandtzæg, Ole Kristian Merkesvik; Johnsen, Elin Follaug; Røberg-Larsen, Hanne; Leknes, Siri; Lundanes, Elsa & Wilson, Steven Ray Haakon (2016). Robust nano liquid chromatography mass spectrometry system for targeted determination of oxytocin in human serum/plasma.
• Røberg-Larsen, Hanne; Lund, Kaja; Sæterdal, Kristina Erikstad; Solheim, Stian; Vehus, Tore & Solberg, Nina [Show all 9 contributors for this article] (2016). Capillary LC mass spectrometric detection of 27-hydroxycholesterol in breast cancer exosomes.
• Sæterdal, Kristina E.; Vehus, Tore; Røberg-Larsen, Hanne; Krauss, Stefan; Lundanes, Elsa & Wilson, Steven Ray Haakon (2016). CYP27A1 – its relation to oxysterols and cancer, and analytical challenges.
• Wilson, Steven Ray Haakon; Lundanes, Elsa; Vehus, Tore; Røberg-Larsen, Hanne; Johnsen, Elin Follaug & Brandtzaeg, Ole Kristian (2015). Ultra-trace analysis of signal pathways: proteomics, lipidomics and neurotransmitters.
• Røberg-Larsen, Hanne; Lund, Kaja; skottvoll, Frøydis S; Krauss, Stefan; Lundanes, Elsa & Wilson, Steven Ray Haakon (2015). Oxysterols in exosomes.
• Røberg-Larsen, Hanne (2015). Highly automatic, sensitive nanoLC based determination of oxysterols in cancer cells.
• Røberg-Larsen, Hanne; Krauss, Stefan; Greibrokk, Tyge; Lundanes, Elsa & Wilson, Steven Ray Haakon (2014). Investigating the Role of Endogenous Small Molecules on the Hedgehog Signal Pathway: Very Sensitive, Highly Automated Nano-LC Based Determination of Oxysterols in Cancer Cells.
• Røberg-Larsen, Hanne; Solberg, Nina; Olsen, Petter Angell; Lund, Kaja; Vesterdal, Caroline & Krauss, Stefan [Show all 8 contributors for this article] (2014). High sensitive, automated quantification of hedgehog active oxysterols in 10 000- 80 000 pancreatic cancer cells using nanoLC-MS.
• Wilson, Steven Ray Haakon; Røberg-Larsen, Hanne; Svendsen, Kristin; Grimsmo, Anders & Lundanes, Elsa (2012). AFFL-SPE-LC: et svitsjesystem som ikke går tett.
• Midtøy, Lise & Røberg-Larsen, Hanne (2023). Evaluation of sample preparation methods for protein analysis of liver organoids. Kjemisk Institutt, Universitetet i Oslo. Show summary

  For mass spectrometry (MS)-based proteomics, different sample preparation methods were evaluated for limited cell samples (10 000-200 000 cells), and the optimized method was used to analyze liver organoids (20-30 organoids, equivalent to approximately 50 000 cells). The sample preparation methods evaluated were urea-based in-solution-digestion (ISD-Urea), a detergent-free sample preparation method named sample preparation by easy extraction and digestion (SPEED), and a method with bead-based sample clean-up called single-pot, solid-phase enhanced sample preparation (SP3). The samples were analyzed with liquid chromatography (LC)-MS using a nanoLC column or a micropillar array column coupled to a trapped ion mobility time of flight (timsTOF)-MS. The samples prepared by SPEED had the overall highest number of protein identifications among all the sample sizes, with a range of 1684-3503 proteins identified for 10 000-100 000 cells. A quantification method for estimation of protein yield was found difficult, but because of the superior performance regarding number of protein identifications, SPEED was chosen as method for analyzing liver organoids of different metabolic states using label-free-quantification. The results showed significant differences between the organoid samples, with many of the hypothesized protein profiles found. The number of protein identifications was in the range of 2140-3203 proteins for the samples consisting of 20-30 organoids each. The method was successfully implemented as a preliminary step for disease studies, e.g. non-alcoholic fatty liver disease (NAFLD).

• Haavaag, Jon Erlend & Røberg-Larsen, Hanne (2023). Towards a high-resolution nano desorption electrospray ionization source for mass spectrometry imaging of organoids. Kjemisk Institutt, Universitetet i Oslo.
• Kvalvik, Eva; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2023). Method Development for Detection of Underivatized Oxysterols in Cell Medium. Kjemisk Institutt. Show summary

  Non-alcoholic fatty liver disease (NAFLD) is a disease caused by the accumulation of fat in the liver, mainly due to obesity and lifestyle. The disease is a worldwide health issue, with high prevalence and a lack of non-invasive biomarkers. To understand the disease, a model system is needed. The use of organoids, stem cell-derived three-dimensional cultures, is suggested as a model reflecting human physiology better than animal models. NAFLD can be induced in liver organoids. This enables the use of “Organ-in-a-Column” technology for studying the disease. Previous research has suggested that oxysterols are potential biomarkers of NAFLD. To make detection easier, oxysterols are usually derivatized in advance of analysis. “Organ-in-a-column” is an on-line system, in which organoids are coupled with Liquid Chromatography – Mass Spectrometry. The on-line approach makes sample preparation in the form of derivatization difficult. Therefore, this study has focused on method development for detecting underivatized oxysterols, with the aim of using the method in an “organ-in-a-column”-setup. Without a derivatization step in the sample preparation, the detection of oxysterols was challenging due to low sensitivity. Attempts of coeluting the groups of hydroxycholesterols and dihydroxycholesterols to establish a steatotic and control fingerprint from organoids with enhanced detection limit were partly successful for the analyte group of dihydroxycholesterols, but not the group of hydroxycholesterols. Sample clean-up was performed on-line using an automated filtration and filter flush solid phase extraction. This ensured robust analysis by the removal of particles before analysis. The optimized experimental parameters included a 5 𝜇L injection volume, a gradient elution utilizing isopropanol (IPA) as the organic modifier (20-65 %), 0.1 % formic acid for pH control in the mobile phase, and the use of a SuperPhenyl hexyl (2.1 mm x 5 cm) column at a temperature of 40 °C. The method allowed for detection of underivatized oxysterols in concentrations down to 0.050 𝜇g/mL in 10:90 IPA:Cell medium. The detection limit was too high to detect oxysterols secreted from liver organoids and the method needs further development before the organ-in-a-column approach can be used for disease monitoring. Also, adjustments to reduce the carry-over would be important to achieve a method providing reliable results.

• Pedersen, Gustav Mathingsdal; Røberg-Larsen, Hanne & Kogler, Stian (2023). Development of On-line Sample Clean-up System for Organ-in-a-Column. Kjemisk Institutt.
• Nordli, Henriette; Wilson, Steven Ray Haakon; Lundanes, Elsa & Røberg-Larsen, Hanne (2021). Towards method miniaturization for determination of sterols in liver organoids: an investigation of nano liquid chromatography-mass spectrometry (nLC-MS). Universitetet i Oslo.
• Moe, Harald Røsand; Wilson, Steven Ray Haakon; Lundanes, Elsa; Røberg-Larsen, Hanne & Lund, Reidar (2021). Establishing a liquid chromatography-mass spectrometry method for the quantification of colistin A, a last line of defense against antibiotic resistance. Universitetet i Oslo. Show summary

  Antibiotic resistance has become an increasingly pressing issue in recent times. With a dwindling discovery rate of new antibacterials, gram-negative bacteria threaten to return humanity to the pre-antibiotic era where mere paper cuts could result in fatal outcomes. The antimicrobial peptide colistin is our present-day last line of defense against gram-negative infections. Nevertheless, despite the recent surge in use and research on colistin, research on its biological effects and chemical behavior remains somewhat scarce. Colistin was initially abandoned due to numerous reports of neuro- and nephrotoxicity. Slow leakage from colistin-loaded liposomes may reduce this toxicity significantly. In this work, an LC-MS method for separating and quantifying colistin and polymyxin B1 was developed to measure colistin leakage from loaded liposomes over time. Isocratic elution provided adequate separation of polymyxin E1 and the internal standard, polymyxin B1, and measurements were done using a triple-quadrupole MS. Widely unreported and uninvestigated adsorption and degradation mechanisms of colistin were observed and addressed by adding a 100 mM ammonium formate buffer at pH 3 to solutions of colistin. The LC-MS method provides a reliable and accurate approach for measuring colistin at lower concentrations (< 10 µg/mL) in ammonium formate buffered solutions, and the future challenges and potential approaches for further research upon colistin are discussed. The LC-MS method described serves as a solid platform for further research on colistin, colistin toxicity negation, and more may be built upon.

• Sande, Maria Therese; Wilson, Steven Ray Haakon; Lundanes, Elsa & Røberg-Larsen, Hanne (2019). Enzymatic hydrolysis with cholesterol esterase in the presence of surfactant improves efficiency for sample preparation of side-chain oxysterols in biosamples. Universitetet i Oslo.
• Kular, Ramneet Kaur; Wilson, Steven Ray Haakon; Røberg-Larsen, Hanne & Lundanes, Elsa (2018). Towards ultrafast liquid chromatography of 3’-phosphoadenosine-5’-phosphosulfate using zwitterionic hydrophilic interaction liquid chromatography-mass spectrometry. Universitetet i Oslo.
• Amundsen, Sunniva Furre; Wilson, Steven Ray Haakon; Berg, Henriette Engen; Røberg-Larsen, Hanne & Lundanes, Elsa (2018). Online entrapment of epitope-containing peptides from integrins using immobilized antibodies. Universitetet i Oslo.
• Solheim, Stian Kjønnås; Lundanes, Elsa; Wilson, Steven Ray Haakon & Røberg-Larsen, Hanne (2018). Exploring selectivity and sensitivity for oxysterol measurements using liquid chromatography-mass spectrometry (LC-MS). Universitetet i Oslo.
• Demir, Deniz; Lundanes, Elsa; Wilson, Steven Ray Haakon; Abele, Silvija & Røberg-Larsen, Hanne (2017). Automatic capillary liquid chromatography tandem mass spectrometry method for pharmaceutical products in environmental water samples. Universitetet i Oslo.
• Skottvoll, Frøydis Sved; Berg, Henriette Engen; Lund, Kaja; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon & Lundanes, Elsa (2017). Critical evaluation of isolation and characterization techniques of breast cancer exosomes. Universitetet i Oslo.
• Røberg-Larsen, Hanne; Lundanes, Elsa; Wilson, Steven Ray Haakon & Krauss, Stefan (2016). High sensitivity measurements of side-chain oxysterols using liquid chromatography mass spectrometry. Universitetet i Oslo. ISSN 1501-7710.
• Sæterdal, Kristina Erikstad; Vehus, Tore; Røberg-Larsen, Hanne; Krauss, Stefan; Lundanes, Elsa & Wilson, Steven Ray Haakon (2016). Determination of CYP27a1 in biological samples using nano liquid chromatography mass spectrometry. Universitetet i Oslo. Show summary

  The cytochrome P450 enzyme, CYP27a1, converts cholesterol into 27-hydroxycholesterol, and both the metabolite and the enzyme are associated with breast cancer, promoting proliferation and metastasis. CYP27A1 is enriched in cancer tissues, and the amount of CYP27a1 correlates to tumor grade. Hence, CYP27a1 is a potential biomarker, and a method for determination and quantification is needed. Antibody assays shows limited performance, and therefore the goal of this work was to develop a nano liquid chromatography mass spectrometry (LC-MS) method for determination of CYP27a1. A sample preparation workflow for CYP27a1 in cell samples was investigated. CYP27a1 was identified by nano LC-MS in MDA-MB-231 breast cancer cells by fractionating cell lysates using gel electrophoresis. Enrichment of CYP27a1 in cell samples using immunoprecipitation, an antibody based method for protein extraction, did not provide adequate identification. However the former method works when looking at other proteins, and this confirms that there are limitations in using antibody based methods for determination and quantification of CYP27a1.

• Vesterdal, Caroline; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon; Greibrokk, Tyge & Lundanes, Elsa (2015). Comparing methods for sensitive determination of Hedgehog active oxysterols. Universitetet i Oslo. Show summary

  Oxysterols in biological samples of limited size are present in low concentrations thus sensitive methods are advantageous. Derivatization of oxysterols prior to liquid chromatography (LC) mass spectrometry (MS) analysis is a common, however a laborious approach. The possibility to reduce the extent of sample preparation by formation of adducts between oxysterols and mobile phase additives (e.g. ammonium acetate) were investigated. Stable adduct ion signals were not obtained with the use of the MS instruments available. However, loss of water ions ([Oxysterol+H-H2O]+ and [Oxysterol+H-2H2O]+, m/z 385.35 and m/z 367.34) were observed when diluting oxysterol standards with ammonium formate (2.5 mM) and formic acid (0.25%) in methanol (MeOH). Under these conditions no easy recognizable fragmentation pattern was observed in tandem MS mode due to clustering of ions in the low mass area when fragmentation energy was applied. Another challenge with the analysis of native oxysterols with a nanoLC system was adsorption to surfaces, especially to fused silica capillaries. Silanization of the fused silica capillaries reduced the issue, but nowhere near satisfactory. It was considered unfeasible to determine native oxysterols with high sensitivity using nanoLC due to the large carry-over issues. Derivatization is therefore recommended when analyzing oxysterols with nanoLC. An on-line automatic filtration filter back-flush solid phase extraction liquid chromatography tandem mass spectrometry (AFFL-SPE-LC-MS/MS) method for determination of Girard T derivatized 24S-hydroxycholesterol, 25-hydroxycholesterol, 27-hydroxycholesterol, and 22S-hydroxycholesterol was modified for analysis of exosome samples. Best separation of oxysterol isomers was obtained with an ACE 3 C18 (0.1 mm ID × 150 mm, 3μm, 100 Å) column with a column temperature of 15°C and by using a mobile phase gradient from 0.1/25/75 (v/v/v %) FA/H2O/MeOH to 0.1/10/90 (v/v/v %) FA/H2O/MeOH in 25 minutes. The method was used for analyses of exosome samples obtained with the use of different isolation techniques to find a suitable procedure for exosome isolation.

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