Protein expression and purification
We have facilities for large-scale recombinant protein production and access to several ÄKTA FPLC systems for protein purification.
Crystal screening and plate imaging
Our facilities have two robots for high-throughput crystal screening (Oryx 4 and Mosquito) and three crystallization hotels/imagers (Formulatrix RI-182, with capacity for 182 plates each) placed in temperature-controlled environments. Results can be accessed remotely in real-time though the RockMaker Web interface.
X-ray data collection
In-house X-ray diffraction data collection is currently unfortunately not possible due to downtime of our Rigaku MacroMax 007 HF single crystal X-ray diffractometer. However, we have regular access to leading international synchrotron radiation facilities such as ESRF (Grenoble), MAX IV (Lund) and DESY (Hamburg), coordinated by UiO for PX-Norway.
In addition, we offer services regarding protein crystal spectroscopy, processing of diffraction data and modeling upon request.
HDX-MS
State-of-the-art hydrogen-deuterium exchange mass spectrometer (HDX-MS, Waters-Synapt G2 Si): Mapping protein folding, conformational changes, protein-ligand and protein-protein interactions.
Biophysical characterization of proteins and protein interactions
A wide range of instruments for characterization of proteins is available at the Department of Medical Biochemistry, Oslo University Hospital. Our equipment includes:
- Microscale thermophoresis instruments (Monolith NT.LabelFree system and NT.115 system for labelled proteins): Quantitative analysis of protein-ligand interactions.
- Isothermal titration calorimeter (MicroCal iTC-200 micro-ITC): Quantitative analysis of protein-ligand and protein-protein interactions.
- Surface Plasmon Resonance (SPR) instrument (GE Biocore S200): Measurement of binding kinetics of protein-ligand and protein-protein interactions.
- Nano-differential scanning fluorimetry (nanoDSF) instrument (Nanotemper, Prometheus NT.48): Determination of thermal and chemical stability of proteins.
- Dynamic light scattering (DLS) system (Malvern Zetasizer Nano-ZS): Measurement of particle size and dispersity of protein solutions.
- SEC-MALS (Malvern Viskotek) : Determination of molecular weight of proteins during size-exclusion chromatography.
In-house SAXS
Small-angle X-ray scattering data can be collected with our Bruker Nanostar SAXS optimized for weak solution scattering, which is part of the RECX instrument park at the Department of Chemistry.
Neutron data collection
PX-Oslo has regular access to world-leading neutron scattering facilities such as the Institut Laue-Langevin (ILL, Grenoble) and the ISIS Neutron and Muon Source (UK).
Prices
We have three different price systems:
- Leiested: Price to access multiple instruments in our facilities, as yearly abonnement or for a full lab space
- Individual prices, Internal: Prices for using individual equipment. Applies to academic users affiliated to the University of Oslo and the Oslo University hospital (OUS)
- Individual prices, External: Use of individual equipment by external users, including industry
- A first consultation is free of charge.
We are open and happy to collaborate! Please contact us for special prices.
Leiested prices
Prices listed have been updated on 28/08/2026
| Abo, Light | Abo, Medium | Abo, Heavy | Full costs per lab space | Location* | |
|---|---|---|---|---|---|
| NFR | 10 KNOK | 25 KNOK | 50 KNOK | 313 KNOK | KI |
| 33.5 KNOK | SAXS-KI (RECX) | ||||
| non-NFR (external projects) | 15 KNOK | 37 KNOK | 74 KNOK | 366 KNOK | KI |
*KI (Department of Chemistry, UiO), NCMM (Centre for Molecular Medicine Norway), IBV (Department of Biosciences, UiO)
Individual prices
| Service | Price (Internal) | Price (External) | Location |
|---|---|---|---|
| Crystallization screens | 800 NOK/screen | 2000 NOK/screen | KI, OUS |
| Instrument time, X-ray diffractometer or protein crystal spectroscopy | 250 NOK/hour | 600 NOK/hour | KI, IBV |
| Instrument time, microscope and liquid nitrogen | 50 NOK/hour | 150 NOK/hour | KI |
| Instrument time, cryo-stream | 150 NOK/hour | 450 NOK/hour | KI |
| Technical support and user training for nanoDSF, SPR, MST, ITC, DLS and FPLC | 700 NOK/hour | 1500 NOK/hour | OUS, KI |
| Unsupervised use of instruments for nanoDSF, SPR, MST, ITC, DLS and FPLC | 200 NOK/hour 1000 NOK/24h | 600 NOK/hour 2400 NOK/24h | OUS, KI |
| Sample handling, characterization, synchrotron data collection, data processing, structural modeling, structural analysis and preparation of material for publication | 700 NOK/hour | 1500 NOK/hour | KI, OUS |
| Recombinant protein production (in E. coli) and purification (customer provides construct; 6 litres standard volume). | 700 NOK/hour + 2500 NOK for materials | 1500 NOK/hour + 2500 NOK for materials | OUS, KI |
| HDX-MS**, basic project (comparison of 2 protein states) | 35 KNOK | 70 KNOK | NCMM |
| HDX-MS**, additional samples | 25 KNOK | 50 KNOK | NCMM |
| Consultation | 700 NOK/hour | 1500 NOK/hour | KI, OUS, IBV, NCMM |
**For HDX-MS services, please contact Nikolina Sekulic. These services include:
- Optimization of sample digestion and generation of “peptide library”
- Preparation of deuterated samples (5-6 time points for each sample)
- Mass-spec data collection for each time point in triplicate (90-110 samples in total)
- Manual analysis of each collected mass-spectra
- Data interpretation and figure generation
Protein crystal spectroscopy services are offered by Hersleth group. For more details, contact Hans-Petter Hersleth.