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Brandtzæg, Ole Kristian Merkesvik; Hustoft, Hanne Kolsrud; Vehus, Tore; Greibrokk, Tyge; Krauss, Stefan & Reubsaet, Leon
[Vis alle 8 forfattere av denne artikkelen]
(2015).
On-line protein digestion and peptide separation using “ultra-nano” LC-MS
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Abele, Silvija; Røgeberg, Magnus; Wilson, Steven Ray Haakon; Lundanes, Elsa & Greibrokk, Tyge
(2014).
Development of new materials for chromatography - HILIC-PLOT columns for LC-MS analysis of metabolites of cancer stem cells.
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Hustoft, Hanne Kolsrud; Wilson, Steven Ray Haakon; Vehus, Tore; Røgeberg, Magnus; Brandtzæg, Ole Kristian Merkesvik & Greibrokk, Tyge
[Vis alle 7 forfattere av denne artikkelen]
(2014).
Open tubular enzyme reactors and LC columns for high performance protein analysis.
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Vehus, Tore; Greibrokk, Tyge; Lundanes, Elsa & Wilson, Steven Ray Haakon
(2014).
Proteomic Analysis of the Wnt/beta-catenin Pathway.
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Røberg-Larsen, Hanne; Krauss, Stefan; Greibrokk, Tyge; Lundanes, Elsa & Wilson, Steven Ray Haakon
(2014).
Investigating the Role of Endogenous Small Molecules on the Hedgehog Signal Pathway: Very Sensitive, Highly Automated Nano-LC Based Determination of Oxysterols in Cancer Cells.
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Vehus, Tore; Røgeberg, Magnus; Grutle, Lene; Greibrokk, Tyge; Wilson, Steven Ray Haakon & Lundanes, Elsa
(2014).
Bottom-up proteomikk med «open layer tubular column» for små prøver – applikasjonar og utvikling.
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Johnsen, Elin Follaug; Folven, Lars; Leknes, Siri; Greibrokk, Tyge; Wilson, Steven Ray Haakon & Lundanes, Elsa
(2014).
On-line coupling of various spe phases with HILIC-LC for enrichment and separation of polar neurotransmitters in various biological matrices.
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Hustoft, Hanne Kolsrud; Brandtzæg, Ole Kristian Merkesvik; Torsetnes, Silje Bøen; Greibrokk, Tyge; Reubsaet, Leon & Wilson, Steven Ray Haakon
[Vis alle 7 forfattere av denne artikkelen]
(2014).
Development of a nanoproteomic platform with integrated immunocapture and enzymatic digestion for on-line detection of the low abundant cancer biomarker PROGRP.
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Røgeberg, Magnus; Grutle, Lene; Wilson, Steven Ray Haakon; Greibrokk, Tyge & Lundanes, Elsa
(2013).
Sensitive, high resolving liquid chromatography using 10 μm I.D. porous layer open tubular (PLOT) columns with Orbitrap mass spectrometry.
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Greibrokk, Tyge
(2013).
From packed LC-columns via SFC to open tubular LC-columns.
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Johnsen, Elin Follaug; Reubsaet, Leon; Stenmark, Harald; Lundanes, Elsa & Greibrokk, Tyge
(2012).
PLOT kolonner med utskiftbar overflate-funksjonalitet basert på gullnanopartikler.
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Malerød, Helle; Hustoft, Hanne Kolsrud; Reubsaet, Leon; Greibrokk, Tyge & Lundanes, Elsa
(2012).
Kan proteiner bli enzymatisk klippet i løpet av minutter med nye og akselererte metoder?
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Røgeberg, Magnus; Wilson, Steven Ray Haakon; Malerød, Helle; Tanaka, Nobou; Lundanes, Elsa & Greibrokk, Tyge
(2012).
Effektiv peptidseparasjon ved bruk av høy temperatur på silikabaserte monolittkolonner.
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Greibrokk, Tyge; Røgeberg, Magnus; Lundanes, Elsa; Misaghian, Dorna & Wilson, Steven Ray Haakon
(2012).
New open tubular columns in HPLC.
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Greibrokk, Tyge; Røgeberg, Magnus; Wilson, Steven Ray Haakon; Lundanes, Elsa; Misaghian, Dorna & Johnsen, Elin Follaug
(2012).
Is there a future for open tubular capillary columns in LC?
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Røgeberg, Magnus; Wilson, Steven Ray Haakon; Lundanes, Elsa & Greibrokk, Tyge
(2011).
Porous Layer Open Tubular Columns for Protein Separations.
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Røgeberg, Magnus; Wilson, Steven Ray Haakon; Malerød, Helle; Tanaka, Nobuo; Lundanes, Elsa & Greibrokk, Tyge
(2011).
Monolithic silica based columns in proteomics: optimization and applications.
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Røgeberg, Magnus; Wilson, Steven Ray Haakon; Malerød, Helle; Lundanes, Elsa & Greibrokk, Tyge
(2011).
Monolithic Silica Based Columns for Fast, High Temperature Peptide Separations in Proteomics.
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Malerød, Helle; Hustoft, Hanne Kolsrud; Wilson, Steven Ray Haakon; Reubsaet, Leon; Lundanes, Elsa & Greibrokk, Tyge
(2011).
Evaluating accelerating methods for trypsin digestion in LC-MS based proteomics.
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Greibrokk, Tyge
(2011).
Recent Advances in two-dimensional capillary liquid chromatography.
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Greibrokk, Tyge; Lundanes, Elsa; Wilson, Steven Ray Haakon & Malerød, Helle
(2010).
Two-dimensional capillary LC.
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Greibrokk, Tyge
(2010).
Multidimensional LC - recent developments.
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Greibrokk, Tyge
(2010).
Open tubular LC - any news?
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Lundanes, Elsa; Røgeberg, Magnus; Wilson, Steven Ray Haakon; Hustoft, Hanne Kolsrud & Greibrokk, Tyge
(2010).
Open tubular liquid chromatography - nanospray MS.
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Hustoft, Hanne Kolsrud; Lundanes, Elsa; Greibrokk, Tyge & Reubsaet, Jan Leo
(2010).
Multidimensjonale nanoLC-MS/MS metoder i jakten på kreftstamcelle markører.
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Malerød, Helle; Lundanes, Elsa & Greibrokk, Tyge
(2010).
Multidimensjonal væskekromatografi og ulike applikasjoner.
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Brenna, Ida; Olsen, Helle; Lundanes, Elsa; Greibrokk, Tyge & Borge, Grethe Iren Andersen
(2010).
Determination of flavenoids in cell cultures and intestinal cells.
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Rahho, Rafika; Hustoft, Hanne Kolsrud; Wilson, Steven Ray Haakon; Lundanes, Elsa & Greibrokk, Tyge
(2010).
Miniaturized capillary columns for protein and peptide analysis.
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Røgeberg, Magnus; Wilson, Steven Ray Haakon; Lundanes, Elsa & Greibrokk, Tyge
(2010).
Open HPLC columns for protein separations.
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Opsal, Kristin; Malerød, Helle; Wilson, Steven Ray Haakon; Lundanes, Elsa & Greibrokk, Tyge
(2010).
Quantification of cancer drugs in blood plasma with LC-UV-MS.
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Johnsen, Elin; Wilson, Steven Ray Haakon; Lundanes, Elsa & Greibrokk, Tyge
(2010).
Method development for determination of (triphosphate) nucleotides on a ZIC-pHILIC column.
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Greibrokk, Tyge & Sellergren, Börje
(2009).
Molecular imprinting in separation science.
Journal of Separation Science.
ISSN 1615-9306.
32,
s. 3263–3264.
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Greibrokk, Tyge; Wilson, Steven Ray Haakon; Malerød, Helle & Lundanes, Elsa
(2009).
Multidimensional capillary liquid chromatography.
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Greibrokk, Tyge; Lundanes, Elsa; Wilson, Steven Ray Haakon; Malerød, Helle; Tzankova, Mihailova Albena & Pepaj, Milaim
(2009).
Multidimensional LC - advantages and limitations.
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Dahl, Sandra Rinne; Lundanes, Elsa; Greibrokk, Tyge & Lea, Tor Erling
(2009).
LC-MS of known and unknown prostaglandions.
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Malerød, Helle; Tzankova, Mihailova Albena; Færgestad, Ellen M; Karaszewski, Bartosz; Hauser, Roman & Lundanes, Elsa
[Vis alle 7 forfattere av denne artikkelen]
(2009).
Neuropeptide profiling in rat hippocampus using capillary 2D-LC-MS/MS.
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Malerød, Helle; Tzankova, Mihailova Albena; Wilson, Steven Ray Haakon; Karaszewski, Bartosz; Hauser, Roman & Lundanes, Elsa
[Vis alle 7 forfattere av denne artikkelen]
(2008).
Comparison of on-line HILIC-RP with on-line SCX-RP for separation of neuropeptides in rat brain.
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Kona, T; Dahl, Sandra Rinne; Lea, Tor Erling; Lundanes, Elsa & Greibrokk, Tyge
(2008).
Capillary liquid chromatography method for determination of adenosine in stem cell culture medium.
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Ellevog, U; Malerød, Helle; Lundanes, Elsa & Greibrokk, Tyge
(2008).
Solubility and chromatography of membrane proteins.
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Dahl, Sandra Rinne; Kleiveland, C.R.; Lea, Tor Erling; Lundanes, Elsa & Greibrokk, Tyge
(2008).
Determination of prostanoids in stem cell culture supernatants.
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Dahl, Sandra Rinne; Kleiveland, C.R; Lundanes, Elsa; Greibrokk, Tyge & Lea, Tor Erling
(2008).
Determination of prostanoids in stem cells – a study to map immunomodulation properties.
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Greibrokk, Tyge; Lundanes, Elsa; Tzankova, Mihailova Albena; Pepaj, Milaim; Tran, Bao Quoc & Wilson, Steven Ray Haakon
[Vis alle 8 forfattere av denne artikkelen]
(2008).
Two-dimensional LC - principles and some applications.
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Greibrokk, Tyge; Lundanes, Elsa; Tzankova, Mihailova Albena; Pepaj, Milaim & Tran, Bao Quoc
(2008).
Miniaturized chromatography at the University of Oslo.
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Greibrokk, Tyge
(2008).
Two dimensional LC principles and some applications.
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Wilson, Steven Ray Haakon; Greibrokk, Tyge; Lundanes, Elsa; Malerød, Helle; Malterud, Karl Egil & Petersen, Dirk
[Vis alle 9 forfattere av denne artikkelen]
(2008).
Identification of the major metal compounds in blepharis aspera.
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Malerød, Helle; Tzankova, Mihailova Albena; Karaszewski, Bartosz; Hauser, Roman; Lundanes, Elsa & Greibrokk, Tyge
(2008).
Determination and identification of neuropeptides in rat hippocampus.
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Chen, Y; Dahl, Sandra Rinne; Malerød, Helle; Lundanes, Elsa & Greibrokk, Tyge
(2008).
Prostaglandins and molecular imprinted polymers.
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Wilson, Steven Ray Haakon; Greibrokk, Tyge; Lundanes, Elsa; Malerød, Helle; Malterud, Karl Egil & Petersen, Dirk
[Vis alle 7 forfattere av denne artikkelen]
(2008).
Identification of the major metal compounds in blepharis aspera.
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Wilson, Steven Ray Haakon; Greibrokk, Tyge; Lundanes, Elsa; Malerød, Helle; Jankowski, Mikolaj & Mihailova, Albena
(2007).
Online two dimensional HILIC-RP separation of peptides.
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Vesterdal, Caroline; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon; Greibrokk, Tyge & Lundanes, Elsa
(2015).
Comparing methods for sensitive determination of Hedgehog active oxysterols.
Universitetet i Oslo.
Vis sammendrag
Oxysterols in biological samples of limited size are present in low concentrations thus sensitive methods are advantageous. Derivatization of oxysterols prior to liquid chromatography (LC) mass spectrometry (MS) analysis is a common, however a laborious approach. The possibility to reduce the extent of sample preparation by formation of adducts between oxysterols and mobile phase additives (e.g. ammonium acetate) were investigated. Stable adduct ion signals were not obtained with the use of the MS instruments available. However, loss of water ions ([Oxysterol+H-H2O]+ and [Oxysterol+H-2H2O]+, m/z 385.35 and m/z 367.34) were observed when diluting oxysterol standards with ammonium formate (2.5 mM) and formic acid (0.25%) in methanol (MeOH). Under these conditions no easy recognizable fragmentation pattern was observed in tandem MS mode due to clustering of ions in the low mass area when fragmentation energy was applied. Another challenge with the analysis of native oxysterols with a nanoLC system was adsorption to surfaces, especially to fused silica capillaries. Silanization of the fused silica capillaries reduced the issue, but nowhere near satisfactory. It was considered unfeasible to determine native oxysterols with high sensitivity using nanoLC due to the large carry-over issues. Derivatization is therefore recommended when analyzing oxysterols with nanoLC. An on-line automatic filtration filter back-flush solid phase extraction liquid chromatography tandem mass spectrometry (AFFL-SPE-LC-MS/MS) method for determination of Girard T derivatized 24S-hydroxycholesterol, 25-hydroxycholesterol, 27-hydroxycholesterol, and 22S-hydroxycholesterol was modified for analysis of exosome samples. Best separation of oxysterol isomers was obtained with an ACE 3 C18 (0.1 mm ID × 150 mm, 3μm, 100 Å) column with a column temperature of 15°C and by using a mobile phase gradient from 0.1/25/75 (v/v/v %) FA/H2O/MeOH to 0.1/10/90 (v/v/v %) FA/H2O/MeOH in 25 minutes. The method was used for analyses of exosome samples obtained with the use of different isolation techniques to find a suitable procedure for exosome isolation.
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Brandtzaeg, Ole Kristian; Hustoft, Hanne Kolsrud; Wilson, Steven Ray Haakon; Greibrokk, Tyge & Lundanes, Elsa
(2014).
Open Tubular Enzyme Reactors (OTERs) for On-line Protein Digestion in Liquid Chromatography Mass Spectrometry Proteomics.
Universitetet i Oslo.
Vis sammendrag
Sample preparation in bottom-up proteomics consists of denaturation, reduction and alkylation of the proteins, before enzymatic digestion of the proteins into peptides. The rate limiting step is the enzymatic digestion, and digestion overnight is recommended. Hence, for faster analysis, the digestion time should be reduced. The main focus of this master thesis was the development of 20 μm inner diameter (ID) open tubular enzyme reactors (OTERs) based on 2-hydroxyethyl methacrylate-co-vinyl azlactone (HEMA-VDM) for on-line protein digestion of limited sample sizes in a nano liquid chromatography (LC) - mass spectrometry (MS) system. Monolithic solid phase extraction (SPE) pre-columns based on butyl methacrylate (BuMa) and polystyrene divinylbenzene (PS-DVB) were prepared in 50 μm ID capillaries and used for trapping of peptides generated by the OTER. These peptides were then separated using 10 μm ID PS-DVB porous layer open tubular (PLOT) columns. The developed OTER was prepared by polymerization using a polymerization mixture consisting of HEMA and VDM as monomer, 1-heptanol or 1-decanol as porogen, and 2,2´azobis(2-methylpropinonitrile) (AIBN) as initiator. Short OTERs were prepared with 1-decanol as porogen, while for longer OTERs, 1-heptanol was used. The sample was loaded onto the OTER with a loading buffer consisting of 50 mM NH4OAc pH 8.75 with 4 % acetonitrile (ACN). A trapping time of 4 min from the OTER to the SPE column was found to be optimal (for the short OTER) using the manual LC-MS-system (developed by Hanne K. Hustoft). The run-to-run retention time repeatability in this system, was 0.25-0.44 % in relative standard deviation (RSD %), and 300 attomoles of targeted recombinant progastrin-releasing peptide isoform 1 (ProGRP) could be detected. The developed long OTER, immobilized with Trypsin/endoproteinase Lys-C (T/L) gave sequence coverages (SQ %) up to 95 % of standard proteins. The optimal reactor temperature during digestion and the optimal digestion time was 37 ºC and 30 min, correspondingly. The OTER could be integrated in an automated LC-MS-system (Hanne K. Hustoft and set up by Tore Vehus), where the within and between digestion repeatability were satisfactory. About 1500 proteins were identified in a single analysis when injecting 1 μg of a human cell lysate sample using the OTER in the automated system.
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