Trine Rounge - UiO/CRN
Ole Herman Ambur - OsloMet
Irene Kraus Christiansen - Ahus
Collaborators:
Pekka Ellonen, Institute for Molecular Medicine Finland (FIMM)
Racheal Mandishora, University of Zimbabwe
Mari Nygård, CRN
Ameli Tropé, CRN
Audrey King, National Institute for Public Health and the Environment (RIVM)
Pascal van der Weele, National Institute for Public Health and the Environment
Ignacio Bravo, Le Centre national de la recherche scientifique (CNRS), France
Background
Human papillomavirus (HPV) is the etiological agent of cervical cancer and other anogenital malignancies and cancer of the head/neck. Typically, HPV infections are transient and clear within relatively short time, typically 1-2 years. Persistent HPV infections, however, may last for years and can lead to the progressive transformation into cancer. Why typically benign infections develop into malign states remains unknown.
More than 200 HPV types are characterized. Of these, fourteen carcinogenic HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 are responsible for nearly all HPV-induced cancers.
Methodology development
High throughput HPV genotyping by NGS technology may increase sensitivity and specificity in both clinical end epidemiological contexts. We have developed a labour and cost effective genotyping protocol with improved analytical sensitivity and specificity. In addition to identifying carcinogenic and other HPV types, our protocol allows the characterization of nucleotide diversity in the target gene relevant for the study of pathogen evolution and vaccine escape.
HPV genotyping for cancer risk prediction is most often based on the gene encoding the viral capsid; the yardstick for HPV taxonomy. However, causal links between viral variants and cancer development are to be found elsewhere in the viral genome. We have therefore developed a HPV whole genome sequencing (HPV-WGS) approach for ultimate resolution in ongoing searches for associations between viral variants and cancer development.
Selected publications
Lagström S, van der Weele P, Rounge TB, Christiansen IK, King AJ, Ambur OH (2019) HPV16 whole genome minority variants in persistent infections from young Dutch women J Clin Virol, 119, 24-30 DOI 10.1016/j.jcv.2019.08.003, PubMed 31446251
Lagström S, Umu SU, Lepistö M, Ellonen P, Meisal R, Christiansen IK, Ambur OH, Rounge TB (2019) TaME-seq: An efficient sequencing approach for characterisation of HPV genomic variability and chromosomal integration Sci Rep, 9 (1), 524 DOI 10.1038/s41598-018-36669-6, PubMed 30679491
Dube Mandishora RS, Gjøtterud KS, Lagström S, Stray-Pedersen B, Duri K, Chin'ombe N, Nygård M, Christiansen IK, Ambur OH, Chirenje MZ, Rounge TB (2018) Intra-host sequence variability in human papillomavirus
Papillomavirus Res, 5, 180-191 DOI 10.1016/j.pvr.2018.04.006, PubMed 29723682
Dube Mandishora RS, Christiansen IK, Chin'ombe N, Duri K, Ngara B, Rounge TB, Meisal R, Ambur OH, Palefsky JM, Stray-Pedersen B, Chirenje ZM (2017)
Genotypic diversity of anogenital human papillomavirus in women attending cervical cancer screening in Harare, Zimbabwe
J Med Virol, 89 (9), 1671-1677 DOI 10.1002/jmv.24825, PubMed 28390142
Meisal R, Rounge TB, Christiansen IK, Eieland AK, Worren MM, Molden TF, Kommedal Ø, Hovig E, Leegaard TM, Ambur OH (2017)
HPV Genotyping of Modified General Primer-Amplicons Is More Analytically Sensitive and Specific by Sequencing than by Hybridization
PLoS One, 12 (1), e0169074 DOI 10.1371/journal.pone.0169074, PubMed 28045981