Roger Simm

• Uhlig, Silvio; Cai, Kun; Liskiewicz, Krystyna Anna; Pain, Maria; Aiko Grutle, Lene & Røberg-Larsen, Hanne [Vis alle 7 forfattere av denne artikkelen] (2024). Profiling of cyclic di-adenyl and -guanyl nucleotides and their precursors and degradation products in bacteria using LC–MS/MS. Separation Science Plus (SSC plus). ISSN 2573-1815. doi: 10.1002/sscp.202400039.
• Frederiksen, Rikki Franklin; Urdahl, Anne Margrete; Simm, Roger; Granstad, Silje; Slettemeås, Jannice Schau & Lagesen, Karin [Vis alle 7 forfattere av denne artikkelen] (2024). Polyether ionophore resistance in a one health perspective. Frontiers in Microbiology. ISSN 1664-302X. 15(1347490), s. 1–11. doi: 10.3389/fmicb.2024.1347490. Fulltekst i vitenarkiv
• Hussain, Badra; Simm, Roger; Bueno, Jaime; Giannettou, Savvas; Naemi, Ali-Oddin & Lyngstadaas, Ståle Petter [Vis alle 7 forfattere av denne artikkelen] (2024). Biofouling on titanium implants: a novel formulation of poloxamer and peroxide for in situ removal of pellicle and multi-species oral biofilm. Regenerative Biomaterials. ISSN 2056-3426. 11. doi: 10.1093/rb/rbae014. Fulltekst i vitenarkiv
• Cimdins, Annika; Naemi, Ali-Oddin; Li, Fengyang; Simm, Roger & Römling, Ute (2023). Characterisation of Variants of Cyclic di-GMP Turnover Proteins Associated with Semi-Constitutive rdar Morphotype Expression in Commensal and Uropathogenic Escherichia coli Strains. Microorganisms. ISSN 2076-2607. 11(8). doi: 10.3390/microorganisms11082048. Fulltekst i vitenarkiv
• Munir, Arooj; Marovic, Danijela; Parreiras Nogueira, Liebert; Simm, Roger; Naemi, Ali-Oddin & Landrø, Sander Marius [Vis alle 14 forfattere av denne artikkelen] (2022). Using Copper-Doped Mesoporous Bioactive Glass Nanospheres to Impart Anti-Bacterial Properties to Dental Composites. Pharmaceutics. ISSN 1999-4923. 14(10). doi: 10.3390/pharmaceutics14102241. Fulltekst i vitenarkiv
• Rørvik, Gro Herredsvela; Naemi, Ali-Oddin; Edvardsen, Per Kristian Thorén & Simm, Roger Gustav (2021). The c-di-AMP signaling system influences stress tolerance and biofilm formation of Streptococcus mitis. MicrobiologyOpen. ISSN 2045-8827. 10(4), s. 1–15. doi: 10.1002/mbo3.1203. Fulltekst i vitenarkiv
• Rørvik, Gro Herredsvela; Liskiewicz, Krystyna Anna; Kryuchkov, Fedor; Naemi, Ali-Oddin; aasheim, Hans-Christian & Petersen, Fernanda Cristina [Vis alle 8 forfattere av denne artikkelen] (2020). Cyclic di-adenosine monophosphate regulates metabolism and growth in the oral commensal streptococcus mitis. Microorganisms. ISSN 2076-2607. 8:1269(9), s. 1–21. doi: 10.3390/microorganisms8091269. Fulltekst i vitenarkiv
• Naemi, Ali-Oddin; Dey, Hymonti; Kiran, Nosheen; Sandvik, Sarah Torbergsen; Slettemeås, Jannice Schau & Nesse, Live Lingaas [Vis alle 7 forfattere av denne artikkelen] (2020). NarAB Is an ABC-Type Transporter That Confers Resistance to the Polyether Ionophores Narasin, Salinomycin, and Maduramicin, but Not Monensin. Frontiers in Microbiology. ISSN 1664-302X. 11:104, s. 1–9. doi: 10.3389/fmicb.2020.00104. Fulltekst i vitenarkiv
• Vestby, Lene Karine; Grønseth, Torstein; Simm, Roger & Nesse, Live Lingaas (2020). Bacterial biofilm and its role in the pathogenesis of disease. Antibiotics. ISSN 2079-6382. 9:59(2), s. 1–29. doi: 10.3390/antibiotics9020059. Fulltekst i vitenarkiv
• Kaspersen, Håkon; Fiskebeck, Eve Marie Louise Zeyl; Sekse, Camilla; Slettemeås, Jannice Schau; Urdahl, Anne Margrete & Norström, Madelaine [Vis alle 8 forfattere av denne artikkelen] (2020). Comparative genome analyses of wild type- and quinolone resistant Escherichia coli indicate dissemination of QREC in the Norwegian broiler breeding pyramid. Frontiers in Microbiology. ISSN 1664-302X. doi: 10.3389/fmicb.2020.00938.
• Kaspersen, Håkon; Sekse, Camilla; Fiskebeck, Eve Marie Louise Zeyl; Slettemeås, Jannice Schau; Simm, Roger & Norström, Madelaine [Vis alle 8 forfattere av denne artikkelen] (2020). Dissemination of Quinolone-Resistant Escherichia coli in the Norwegian Broiler and Pig Production Chains and Possible Persistence in the Broiler Production Environment. Applied and Environmental Microbiology. ISSN 0099-2240. doi: 10.1128/AEM.02769-19. Fulltekst i vitenarkiv
• Simm, Roger; Slettemeås, Jannice Schau; Norström, Madelaine; Dean, Katharine Rose; Kaldhusdal, Magne & Urdahl, Anne Margrete (2019). Significant reduction of vancomycin resistant E. faecium in the Norwegian broiler population coincided with measures taken by the broiler industry to reduce antimicrobial resistant bacteria. PLOS ONE. ISSN 1932-6203. 14(12). doi: 10.1371/journal.pone.0226101. Fulltekst i vitenarkiv
• Paulsen, Britt; Fredriksen, Kim Alex; Petersen, Dirk; Maes, Louis; Matheeussen, An & Naemi, Ali-Oddin [Vis alle 11 forfattere av denne artikkelen] (2019). Synthesis and antimicrobial activities of N6-hydroxyagelasine analogs and revision of the structure of ageloximes. Bioorganic & Medicinal Chemistry. ISSN 0968-0896. 27(4), s. 620–629. doi: 10.1016/j.bmc.2019.01.002. Fulltekst i vitenarkiv Vis sammendrag

  (+)-N6-Hydroxyagelasine D, the enantiomer of the proposed structure of (–)-ageloxime D, as well as N6-hydroxyagelasine analogs were synthesized by selective N-7 alkylation of N6-[tert-butyl(dimethyl)silyloxy]-9-methyl-9H-purin-6-amine in order to install the terpenoid side chain, followed by fluoride mediated removal of the TBDMS-protecting group. N6-Hydroxyagelasine D and the analog carrying a geranylgeranyl side chain displayed profound antimicrobial activities against several pathogenic bacteria and protozoa and inhibited bacterial biofilm formation. However these compounds were also toxic towards mammalian fibroblast cells (MRC-5). The spectral data of N6-hydroxyagelasine D did not match those reported for ageloxime D before. Hence, a revised structure of ageloxime D was proposed. Basic hydrolysis of agelasine D gave (+)-N-[4-amino-6-(methylamino)pyrimidin-5-yl]-N-copalylformamide, a compound with spectral data in full agreement with those reported for (–)-ageloxime D.

• Enersen, Morten; Hultin, Margareta; Kønønen, Eija; Havemose Poulsen, Anne & Simm, Roger (2019). Antibiotics in the treatment of periodontal and peri-implant infections. Tandlægebladet. ISSN 0039-9353. 129(3), s. 224–235.
• Nesse, Live Lingaas & Simm, Roger (2018). Biofilm: A Hotspot for Emerging Bacterial Genotypes. Advances in Applied Microbiology. ISSN 0065-2164. 103, s. 223–246. doi: 10.1016/bs.aambs.2018.01.003. Vis sammendrag

  Bacteria have the ability to adapt to changing environments through rapid evolution mediated by modification of existing genetic information, as well as by horizontal gene transfer (HGT). This makes bacteria a highly successful life form when it comes to survival. Unfortunately, this genetic plasticity may result in emergence and dissemination of antimicrobial resistance and virulence genes, and even the creation of multiresistant “superbugs” which may pose serious threats to public health. As bacteria commonly reside in biofilms, there has been an increased interest in studying these phenomena within biofilms in recent years. This review summarizes the present knowledge within this important area of research. Studies on bacterial evolution in biofilms have shown that mature biofilms develop into diverse communities over time. There is growing evidence that the biofilm lifestyle may be more mutagenic than planktonic growth. Furthermore, all three main mechanisms for HGT have been observed in biofilms. This has been shown to occur both within and between bacterial species, and higher transfer rates in biofilms than in planktonic cultures were detected. Of special concern are the observations that mutants with increased antibiotic resistance occur at higher frequency in biofilms than in planktonic cultures even in the absence of antibiotic exposure. Likewise, efficient dissemination of antimicrobial resistance genes, as well as virulence genes, has been observed within the biofilm environment. This new knowledge emphasizes the importance of biofilm awareness and control.

• Kaspersen, Håkon; Urdahl, Anne Margrete; Simm, Roger; Slettemeås, Jannice Schau; Lagesen, Karin & Norström, Madelaine (2018). Occurrence of quinolone resistant E. coli originating from different animal species in Norway. Veterinary Microbiology. ISSN 0378-1135. 217, s. 25–31. doi: 10.1016/j.vetmic.2018.02.022. Fulltekst i vitenarkiv
• Cimdins, Annika; Simm, Roger; Li, Fengyang; Lüthje, Petra; Thorell, Kaisa & Sjöling, Åsa [Vis alle 8 forfattere av denne artikkelen] (2017). Alterations of c-di-GMP turnover proteins modulate semi-constitutive rdar biofilm formation in commensal and uropathogenic Escherichia coli. MicrobiologyOpen. ISSN 2045-8827. 6(5). doi: 10.1002/mbo3.508.
• Hassan, Karl A.; Fagerlund, Annette; Elbourne, Liam D.H.; Vörös, Aniko; Kroeger, Jasmin Kaori & Simm, Roger [Vis alle 12 forfattere av denne artikkelen] (2017). The putative drug efflux systems of the Bacillus cereus group. PLOS ONE. ISSN 1932-6203. 12(5), s. 1–25. doi: 10.1371/journal.pone.0176188. Fulltekst i vitenarkiv Vis sammendrag

  The Bacillus cereus group of bacteria includes seven closely related species, three of which, B. anthracis, B. cereus and B. thuringiensis, are pathogens of humans, animals and/ or insects. Preliminary investigations into the transport capabilities of different bacterial lineages suggested that genes encoding putative efflux systems were unusually abundant in the B. cereus group compared to other bacteria. To explore the drug efflux potential of the B. cereus group all putative efflux systems were identified in the genomes of prototypical strains of B. cereus, B. anthracis and B. thuringiensis using our Transporter Automated Annotation Pipeline. More than 90 putative drug efflux systems were found within each of these strains, accounting for up to 2.7% of their protein coding potential. Comparative analyses demonstrated that the efflux systems are highly conserved between these species; 70± 80% of the putative efflux pumps were shared between all three strains studied. Furthermore, 82% of the putative efflux system proteins encoded by the prototypical B. cereus strain ATCC 14579 (type strain) were found to be conserved in at least 80% of 169 B. cereus group strains that have high quality genome sequences available. However, only a handful of these efflux pumps have been functionally characterized. Deletion of individual efflux pump genes from B. cereus typically had little impact to drug resistance phenotypes or the general fitness of the strains, possibly because of the large numbers of alternative efflux systems that may have overlapping substrate specificities. Therefore, to gain insight into the possible transport functions of efflux systems in B. cereus, we undertook large-scale qRTPCR analyses of efflux pump gene expression following drug shocks and other stress treatments. Clustering of gene expression changes identified several groups of similarly regulated systems that may have overlapping drug resistance functions. In this article we review current knowledge of the small molecule efflux pumps encoded by the B. cereus group and suggest the likely functions of numerous uncharacterised pumps.

• Simm, Roger; Kvalvaag, Audun Sverre Myrset; van Deurs, Bo; Lindback, Toril & Sandvig, Kirsten (2017). Benzyl alcohol induces a reversible fragmentation of the Golgi apparatus and inhibits membrane trafficking between endosomes and the trans-Golgi network. Experimental Cell Research. ISSN 0014-4827. 357(1), s. 67–78. doi: 10.1016/j.yexcr.2017.04.025.
• Kroeger, Jasmin Kaori; Hassan, Karl; Vörös, Aniko; Simm, Roger; Saidijam, Massoud & Bettaney, Kim E. [Vis alle 10 forfattere av denne artikkelen] (2015). Bacillus cereus efflux protein BC3310 - A multidrug transporter of the unknown major facilitator family, UMF-2. Frontiers in Microbiology. ISSN 1664-302X. 6. doi: 10.3389/fmicb.2015.01063.
• Kymre, Linn Furebotten; Simm, Roger; Skotland, Tore & Sandvig, Kirsten (2015). Different roles of the C-terminal end of Stx1A and Stx2A for AB5 complex integrity and retrograde transport of Stx in HeLa cells. Pathogens and Disease. ISSN 2049-632X. 73(9), s. 1–9. doi: 10.1093/femspd/ftv083.
• Kavaliauskiene, Simona; Skotland, Tore; Sylvänne, Tuulia; Simolin, Helena; Klokk, Tove Irene & Torgersen, Maria Lyngaas [Vis alle 10 forfattere av denne artikkelen] (2015). Novel actions of 2-deoxy-D-glucose: Protection against Shiga toxins and changes in cellular lipids. Biochemical Journal. ISSN 0264-6021. 470, s. 23–37. doi: 10.1042/BJ20141562.
• Le Guyon, Soazig; Simm, Roger; Rehn, Mikael & Römling, Ute (2015). Dissecting the cyclic di-guanylate monophosphate signalling network regulating motility in Salmonella enterica serovar Typhimurium. Environmental Microbiology. ISSN 1462-2912. 17(4), s. 1310–1320. doi: 10.1111/1462-2920.12580.
• Lingelem, Anne Berit Dyve; Hjelseth, Ieva Ailte; Simm, Roger; Torgersen, Maria Lyngaas & Sandvig, Kirsten (2015). Geldanamycin enhances retrograde transport of Shiga toxin in HEp-2 cells. PLOS ONE. ISSN 1932-6203. 10:e0129214(5). doi: 10.1371/journal.pone.0129214.
• Vörös, Aniko; Simm, Roger; Slamti, Leyla; McKay, Matthew J.; Hegna, Ida Kristin & Nielsen-Leroux, Christina [Vis alle 12 forfattere av denne artikkelen] (2014). SecDF as part of the sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins. PLOS ONE. ISSN 1932-6203. 9:e0103326(8). doi: 10.1371/journal.pone.0103326.
• Simm, Roger; Ahmad, Irfan; Rhen, Mikael; Guyon, Soazig Le & Römling, Ute (2014). Regulation of biofilm formation in Salmonella enterica serovar Typhimurium. Future Microbiology. ISSN 1746-0913. 9(11), s. 1261–1282. doi: 10.2217/fmb.14.88.
• Bergan, Jonas; Skotland, Tore; Anne Berit Dyve, Lingelem; Simm, Roger; Spilsberg, Bjørn & Lindback, Toril [Vis alle 10 forfattere av denne artikkelen] (2014). The ether lipid precursor hexadecylglycerol protects against Shiga toxins. Cellular and Molecular Life Sciences (CMLS). ISSN 1420-682X. 71, s. 4285–4300. doi: 10.1007/s00018-014-1624-1.
• Kavaliauskiene, Simona; Nymark, Carl-Martin; Bergan, Jonas; Simm, Roger; Sylvänne, Tuulia & Simolin, Helena [Vis alle 9 forfattere av denne artikkelen] (2014). Cell density-induced changes in lipid composition and intracellular trafficking. Cellular and Molecular Life Sciences (CMLS). ISSN 1420-682X. 71(6), s. 1097–1116. doi: 10.1007/s00018-013-1441-y.
• Vörös, Aniko; Simm, Roger; Kroeger, Jasmin Kaori & Kolstø, Anne-Brit (2013). Gene transcription from the linear plasmid pBClin15 leads to cell lysis and extracellular DNA-dependent aggregation of Bacillus cereus ATCC 14579 in response to quinolone-induced stress. Microbiology (Reading). ISSN 1350-0872. 159, s. 2283–2293. doi: 10.1099/mic.0.069674-0.
• Backe, Paul Hoff; Simm, Roger; Lærdahl, Jon Kristen; Dalhus, Bjørn; Fagerlund, Annette & Økstad, Ole Andreas Løchen [Vis alle 10 forfattere av denne artikkelen] (2013). A new family of proteins related to the HEAT-like repeat DNA glycosylases with affinity for branched DNA structures. Journal of Structural Biology. ISSN 1047-8477. 183(1), s. 66–75. doi: 10.1016/j.jsb.2013.04.007. Fulltekst i vitenarkiv
• Kvalvaag, Audun Sverre Myrset; Pust, Sascha; Sundet, Kristine Ingrid; Simm, Roger; Sandvig, Kirsten & Engedal, Kim Nikolai (2013). The ERM Proteins Ezrin and Moesin Regulate Retrograde Shiga Toxin Transport. Traffic: The moving front of cell biology. ISSN 1398-9219. 14(7), s. 839–852. doi: 10.1111/tra.12077.
• Simm, Roger; Vörös, Aniko; Ekman, Jaakko V; Sødring, Marianne Sundt; Nes, Ingerid Veslemøy & Kroeger, Jasmin Kaori [Vis alle 11 forfattere av denne artikkelen] (2012). BC4707 Is a Major Facilitator Superfamily Multidrug Resistance Transport Protein from Bacillus cereus Implicated in Fluoroquinolone Tolerance. PLOS ONE. ISSN 1932-6203. 7(5). doi: 10.1371/journal.pone.0036720.
• Bergan, Jonas; Lingelem, Anne Berit Dyve; Simm, Roger; Skotland, Tore & Sandvig, Kirsten (2012). Shiga toxins. Toxicon. ISSN 0041-0101. 60(6), s. 1085–1107. doi: 10.1016/j.toxicon.2012.07.016.

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• Hussain, Badra; Simm, Roger; Bueno, Jaime; Giannettou, Savvas; Naemi, Ali-Oddin & Lyngstadaas, Ståle Petter [Vis alle 7 forfattere av denne artikkelen] (2023). Chemical decontamination strategies for treatment of peri-implant disease.
• Hussain, Badra; Simm, Roger; Lyngstadaas, Ståle Petter; Bueno, Jamie & Haugen, Håvard Jostein (2023). Chemical decontamination strategies for treatment of peri-implant disease: using an in vitro dental pellicle-, and a multispecies biofilm model.
• Liskiewicz, Krystyna Anna; Araya, Feven Tesfaldet; Vallenari, Evan Michael; Parajuli, Himalaya; Ramstad, Silje & Schreurs, Olaf Joseph Franciscus [Vis alle 8 forfattere av denne artikkelen] (2023). Cyclic-di-adenosine monophosphate’s effect on virulence of Streptococcus mitis CCUG 31611.
• Cai, Kun; Liskiewicz, Krystyna Anna; Sribasgaran, Biramitha; Naemi, Ali-Oddin & Simm, Roger (2023). The role of trkA and c-di-AMP in Streptococcus mitis.
• Simm, Roger (2023). C-di-AMP signaling regulates biofilm formation, stress tolerance and virulence in Streptococcus mitis.
• Simm, Roger; Urdahl, Anne Margrete; Stassen, Joost & Pikkemaat, mariel (2023). Use of ionophores in poultry feed. The Veterinary Record. ISSN 0042-4900. doi: 10.1002/vetr.2710.
• Simm, Roger; Norström, Madelaine; Slettemeås, Jannice Schau & Urdahl, Anne Margrete (2019). No vancomycin resistant enterococci (VRE) detected in the Norwegian poultry Production for the first time since the monitoring started. I Simonsen, Gunnar Skov; Blix, Hege Salvesen; Grave, Kari & Urdahl, Anne Margrete (Red.), NORM/NORM-VET 2018 Usage of Antimicrobial Agents and Occurrence of Antimicrobial Resistance in Norway. NORM/NORM-VET. ISSN 1502-2307.
• Simm, Roger (2019). Biofilms and antibiotic resistance: a curse of modern medicine.
• Liskiewicz, Krystyna Anna; Sunde, Pia Titterud; Verket, Anders; Økstad, Ole Andreas & Simm, Roger (2019). C-di-GMP signaling in Treponema denticola and its role in pathogenesis of periodontal diseases.
• Liskiewicz, Krystyna Anna; Sunde, Pia Titterud; Verket, Anders; Økstad, Ole Andreas & Simm, Roger (2019). C-di-GMP signaling in Treponema denticola and its role in pathogenesis of periodontal diseases.
• Kaspersen, Håkon; Sekse, Camilla; Slettemeås, Jannice Schau; Simm, Roger; Norström, Madelaine & Urdahl, Anne Margrete [Vis alle 7 forfattere av denne artikkelen] (2019). Low-resolution MLST as an initial analysis for deep characterization of quinolone resistant Escherichia coli. Vis sammendrag

  We are currently investigating the presence of putative antimicrobial resistance genes (ARG) and population structure in a set of quinolone resistant E. coli (QREC) from Norway, and as part of that study we are further investigating specific sequence types (ST). The goal of this study was to analyse clustering of isolates from selected STs, by utilizing whole genome MLST in addition to ARGs, virulence genes, and serotypes, to investigate the similarity between isolates from different animal species. A total of 285 QREC isolates originating from broilers, pigs, red foxes and wild birds were subjected to whole genome sequencing. ARGs were identified with ARIBA, with the MegaRes (intrinsic genes) and ResFinder (acquired genes) databases. Core genome multi locus sequence typing (cgMLST) with chewBBACA using the Enterobase scheme for E. coli was used for distance calculation and tree generation, and the regular sequence types (ST) were determined by the Escherichia coli #1 Enterobase scheme. STs which are represented with at least 10 isolates and that are present in at least three animal species was selected for serotyping, virulence gene identification and whole genome MLST (wgMLST) by using SeroTypeFinder, VirulenceFinder, and chewBBACA, respectively. Based on this, sequence types 10, 58, 155 and 162 were selected for further analyses. Some sequence types had a more homogenous distribution of ARGs than others. In regard to ARGs, serotypes, virulence genes and the wgMLST results, a higher diversity was seen in ST10 isolates. Serotypes clustered in concordance with wgMLST results, especially in ST162 and ST155.

• Paulsen, Britt; Fredriksen, Kim Alex; Petersen, Dirk; Maes, Louis; Matheeussen, An & Naemi, Ali-Oddin [Vis alle 11 forfattere av denne artikkelen] (2019). Synthesis directed towards bioactive ent-Ageloxime D and analogues.
• Paulsen, Britt; Fredriksen, Kim Alex; Petersen, Dirk; Maes, Louis; Matheeussen, An & Naemi, Ali-Oddin [Vis alle 10 forfattere av denne artikkelen] (2019). Synthesis directed towards bioactive ent-Ageloxime D and analogues.
• Kaspersen, Håkon; Sekse, Camilla; Slettemeås, Jannice Schau; Simm, Roger; Norström, Madelaine & Sørum, Henning [Vis alle 8 forfattere av denne artikkelen] (2018). Quinolone resistance mechanisms in E. coli from four animal species in Norway.
• Kaspersen, Håkon; Urdahl, Anne Margrete; Slettemeås, Jannice Schau; Simm, Roger; Sørum, Henning & Lagesen, Karin [Vis alle 7 forfattere av denne artikkelen] (2017). Quinolone resistance despite low antimicrobial usage: comparison of occurrence in different species in Norway the last ten years .
• Vörös, Aniko; Simm, Roger; Schleimer, Jasmin Kaori; Tourasse, Nicolas; Henderson, Peter J. F. & Kolstø, Anne-Brit (2009). Functional characterization of putative multidrug resistance transporters conserved in the Bacillus cereus group.
• Sandvig, Kirsten; Simm, Roger & Kymre, LInn (2014). Structure-function relationship of Shiga toxins. Role of the A-subunit in complex stability and endocytosis. 7Letras.

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