katribje

• Bjerkan, Katrine Nergård; Alling, Renate; Myking, Ida Velle; Brysting, Anne Krag & Grini, Paul (2023). Genetic and environmental manipulation of Arabidopsis hybridization barriers uncovers antagonistic functions in endosperm cellularization. Frontiers in Plant Science. ISSN 1664-462X. 14, p. 1–16. doi: 10.3389/fpls.2023.1229060. Full text in Research Archive
• Bramsiepe, Jonathan; Krabberød, Anders Kristian; Bjerkan, Katrine Nergård; Alling, Renate; Nielsen, Ida & Hornslien, Karina Stensland [Show all 9 contributors for this article] (2023). Structural evidence for MADS-box type I family expansion seen in new assemblies of Arabidopsis arenosa and A. lyrata. The Plant Journal. ISSN 0960-7412. 116(3), p. 942–961. doi: 10.1111/tpj.16401. Full text in Research Archive
• van Ekelenburg, Yuri Stephan; Hornslien, Karina Stensland; Hautegem, Tom Van; Fendrych, Matyáš; Van Isterdael, Gert & Bjerkan, Katrine Nergård [Show all 9 contributors for this article] (2022). Spatial and temporal regulation of parent-of-origin allelic expression in the endosperm. Plant Physiology. ISSN 0032-0889. 191(2), p. 986–1001. doi: 10.1093/plphys/kiac520. Full text in Research Archive Show summary

  Genomic imprinting promotes differential expression of parental alleles in the endosperm of flowering plants and is regulated by epigenetic modification such as DNA methylation and histone tail modifications in chromatin. After fertilization, the endosperm develops through a syncytial stage before it cellularizes and becomes a nutrient source for the growing embryo. Regional compartmentalization has been shown both in early and late endosperm development, and different transcriptional domains suggest divergent spatial and temporal regional functions. The analysis of the role of parent-of-origin allelic expression in the endosperm as a whole and the investigation of domain-specific functions have been hampered by the inaccessibility of the tissue for high-throughput transcriptome analyses and contamination from surrounding tissue. Here, we used fluorescence-activated nuclear sorting (FANS) of nuclear targeted GFP fluorescent genetic markers to capture parental-specific allelic expression from different developmental stages and specific endosperm domains. This approach allowed us to successfully identify differential genomic imprinting with temporal and spatial resolution. We used a systematic approach to report temporal regulation of imprinted genes in the endosperm, as well as region-specific imprinting in endosperm domains. Analysis of our data identified loci that are spatially differentially imprinted in one domain of the endosperm, while biparentally expressed in other domains. These findings suggest that the regulation of genomic imprinting is dynamic and challenge the canonical mechanisms for genomic imprinting.

• Bjerkan, Katrine; Hornslien, Karina Stensland; Johannessen, Ida Marie; Krabberød, Anders Kristian; van Ekelenburg, Yuri Stephan & Kalantarian, Maryam [Show all 11 contributors for this article] (2019). Genetic variation and temperature affects hybrid barriers during interspecific hybridization. The Plant Journal. ISSN 0960-7412. 101, p. 122–140. doi: 10.1111/tpj.14523. Full text in Research Archive
• Lafon-Placette, Clément; Johannessen, Ida Marie; Hornslien, Karina Stensland; Ali, Mohammad F.; Bjerkan, Katrine & Bramsiepe, Jonathan [Show all 11 contributors for this article] (2017). Endosperm-based hybridization barriers explain the pattern of gene flow between Arabidopsis lyrata and Arabidopsis arenosa in Central Europe. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 114(6), p. E1027–E1035. doi: 10.1073/pnas.1615123114.
• Bjerkan, Katrine & Grini, Paul Eivind (2013). The Arabidopsis DDB1 interacting protein WDR55 is required for vegetative development. Plant Signalling & Behavior. ISSN 1559-2316. 8(9). doi: 10.4161/psb.25347.
• Gregis, Veronica; Andrès, Fernandio; Sessa, Alice; Guerra, Rosalinda F; Simonini, Sara & Mateos, Julieta L [Show all 15 contributors for this article] (2013). Identification of pathways directly regulated by SHORT VEGETATIVE PHASE during vegetative and reproductive development in Arabidopsis. Genome Biology. ISSN 1465-6906. 14(6). doi: 10.1186/gb-2013-14-6-r56.
• Bjerkan, Katrine; Jung-Romeo, S; Jurgens, G; Genschik, P & Grini, Paul Eivind (2012). Arabidopsis WD REPEAT DOMAIN55 Interacts with DNA DAMAGED BINDING PROTEIN1 and Is Required for Apical Patterning in the Embryo. The Plant Cell. ISSN 1040-4651. 24(3), p. 1013–1033. doi: 10.1105/tpc.111.089425.
• Shirzadi, Reza; Andersen, Ellen Dehnes; Bjerkan, Katrine; Gloeckle, Barbara M; Heese, Maren & Ungru, Alexander [Show all 11 contributors for this article] (2011). Genome-Wide Transcript Profiling of Endosperm without Paternal Contribution Identifies Parent-of-Origin-Dependent Regulation of AGAMOUS-LIKE36. PLoS Genetics. ISSN 1553-7390. 7(2). doi: 10.1371/journal.pgen.1001303. Full text in Research Archive
• Nowack, MK; Ungru, Alexander; Bjerkan, Katrine; Grini, Paul Eivind & Schnittger, Arp (2010). Reproductive cross-talk: seed development in flowering plants. Biochemical Society Transactions. ISSN 0300-5127. 38, p. 604–612. doi: 10.1042/BST0380604.

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• Alling, Renate; Brysting, Anne Krag; Grini, Paul; Bjerkan, Katrine & Myking, Ida Velle (2022). Environmental and genetic factors affecting endosperm based post-zygotic hybridization barriers. Show summary

  Proper development of the triploid endosperm is vital for seed viability. During the early stages of development, the endosperm works as a nutrient sink, accumulating sugars from the surrounding tissues. During this developmental phase, endosperm division is devoid of cytokinesis, resulting in a syncytium. After sufficient accumulation of nutrients, the endosperm nuclei undergo cellularization, switching to serve as a nutrient source for the embryo. Tight regulation of cellularization is therefore vital for embryo development. Crosses between Arabidopsis thaliana as maternal contributor and A. arenosa as pollen donor cause late cellularization and reduced germination rate in hybrid seeds. Here we show the opposite cellularization phenotype in hybrid seeds when using A. lyrata as pollen donor also resulting in low germination rate. Stage specific endosperm reporters display ectopic expression in developing hybrid endosperm confirming disturbance of the source-sink endosperm phase change. We also observe accession genotype as well as single loci effects on the severity of the barrier. Interestingly, we found that temperature can alleviate this hybridization barrier in opposite directions depending on whether the hybrid seed exhibit early or late endosperm cellularization. We postulate that temperature dependency might be a general mechanism affecting endosperm-based hybridization barriers.

• Bjerkan, Katrine; Butenko, Melinka Alonso & Brysting, Anne Krag (2020). The weed that conquered plant scientists: the model organism Arabidopsis thaliana. NBS-nytt. ISSN 0801-3535. 1, p. 8–11.
• Kenich, Håkon Muland; Bjerkan, Katrine; Bramsiepe, Jonathan; Grini, Paul Eivind & Brysting, Anne Krag (2019). An investigation of potential hybridisation barriers in Cochlearia / Scurvygrasses.
• Bjerkan, Katrine; Hornslien, Karina Stensland; Johannessen, Ida Marie; van Ekelenburg, Yuri Stephan; Bramsiepe, Jonathan & Brysting, Anne Krag [Show all 7 contributors for this article] (2019). Temperature and genetic variation alter strength of the post-zygotic endosperm based barrier between A. thaliana and A.arenosa.
• Grini, Paul Eivind; van Ekelenburg, Yuri Stephan; Bjerkan, Katrine & Nowack, Moritz K. (2018). Analysis of Domain Specific Genomic Imprinting in the Arabidopsis Endosperm.
• Johannessen, Ida Marie; Lafon-Placette, Clément; Hornslien, Karina Stensland; Bramsiepe, Jonathan; Bjerkan, Katrine & Brysting, Anne Krag [Show all 8 contributors for this article] (2017). The effect of gene and genome dosage on hybridization barriers in Arabidopsis lyrata and Arabidospsis arenosa.

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