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Bjerkan, Katrine; Butenko, Melinka Alonso & Brysting, Anne Krag
(2020).
The weed that conquered plant scientists: the model organism Arabidopsis thaliana.
NBS-nytt.
ISSN 0801-3535.
1,
p. 8–11.
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Hubin, Aliaksandr; Storvik, Geir Olve; Grini, Paul Eivind & Butenko, Melinka Alonso
(2019).
Bayesian binomial regression model with a latent Gaussian field for analysis of epigenetic data.
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Thorstensen, Tage; Lie Andersen, Heidi; Hamann, Thorsten & Butenko, Melinka Alonso
(2017).
Uvitenskapelig motstand mot genredigering av matplanter.
Nationen.
ISSN 0805-3782.
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Herrmann, Ullrich; WILDHAGEN, Mari; Stø, Ida Myhrer; Butenko, Melinka Alonso & Aalen, Reidunn B.
(2014).
Secreted IDA-LIKE peptide ligands and their receptors regulate crucial aspects of root development – root cap sloughing, gravitropism and meristem length.
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Herrmann, Ullrich; WILDHAGEN, Mari; Butenko, Melinka Alonso & Aalen, Reidunn B.
(2014).
Small peptides are involved in cell separation processes in the root.
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Vie, Ane Kjersti; Najafi, Javad; Liu, Bin; Winge, Per; Butenko, Melinka Alonso & Hornslien, K
[Show all 10 contributors for this article]
(2012).
Expression of the extended IDA gene family in Arabidopsis thaliana: evidence for post-transcriptional regulation by mRNA decapping.
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Butenko, Melinka Alonso; Shi, Chunlin & Aalen, Reidunn B.
(2010).
Class I KNOX proteins involved in floral abscission downstream in the IDA-HAE/HSL2 signaling pathway.
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Aalen, Reidunn B.; Stenvik, Grethe-Elisabeth; Shi, Chunlin & Butenko, Melinka Alonso
(2009).
Identification of putative peptide ligands and receptors involved in control of cell-separation processes in plants.
Show summary
The novel ligand/receptor pair, IDA and the closely related receptor-like kinases (RLKs) HAESA (HAE) and HAESA-LIKE 2 (HSL2), controls floral organ abscission in Arabidopsis. The IDA gene encodes a protein of 77 amino acids (aa), with a N-terminal signal sequence exporting the protein. Genes encoding IDA-LIKE proteins, that share a common C-terminal 12aa motif (PIP), are present both in Arabidopsis and other plant species. The active IDA peptide resided within an extended PIP motif (EPIP). IDA-LIKE (IDL) proteins can in part substitute for IDA function. The IDL expression patterns indicate involvement in regulation of various cell separation events, e.g. root cap sloughing and seed dispersal. Plants constitutively overexpressing IDA exhibit earlier abscission of floral organs and ectopic abscission of flowers, branches and cauline leaves and overexpression of the IDL proteins mimic this phenotype. The over-expression phenotype is dependent on functional HAE and HSL2 RLKs. We suggest that the IDL peptides interact with HEASA-LIKE receptors to control cell-separation processes, and that new peptide-ligand/receptor pairs may be identified by a hypothesis-driven approach with the aid of genetics and new methods to identify active peptides and direct peptide-RLK interactions.
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Aalen, Reidunn B.; Stenvik, Grethe-Elisabeth; Butenko, Melinka Alonso & Shi, Chunlin
(2009).
Identification of putative peptide ligands and receptors involved in control of cell-separation processes in plants.
Show summary
Recently a novel ligand/receptor pair, IDA and the closely related receptor-like kinases (RLKs) HAESA (HAE) and HAESA-LIKE 2 (HSL2), was revealed to control floral organ abscission in Arabidopsis (Stenvik et al., 2008, Plant Cell, 20: 1805-1817). The IDA gene encodes a protein of only 77 amino acids (aa), with a N-terminal signal sequence that exports the protein out of the cell. There is evidence suggesting that the protein is processed to release a small peptide from the C-terminal end. Genes encoding IDA-LIKE proteins have been identified both in Arabidopsis and other plant species. They are all less than 100 aa, with a N-terminal signal peptide and a common C-terminal motif called PIP (Butenko et al., 2003, Plant Cell, 15: 2296-2307). The active IDA peptide resided within an extended PIP motif (EPIP) of 20 aa (Stenvik et al., 2008). In Arabidopsis IDA-LIKE (IDL) proteins can in part substitute for IDA function. Promoter:GUS expression studies of the IDL genes indicate that these genes may be involved in regulating various cell separation events such as root cap sloughing, stomata development and formation of hydathodes. A 35S:IDA line constitutively overexpressing IDA exhibit earlier abscission of floral organs and ectopic abscission at the bases of the pedicel, branches of the inflorescence, and cauline leaves (Stenvik et al. 2006, Plant Cell 18: 1467-1476) and overexpression of the IDL proteins mimic this phenotype (Stenvik et al., 2008). The over-expression phenotype is dependent on functional HAE and HSL2 RLKs. We suggest that the IDL peptides interact with HEASA-LIKE receptors to control cell-separation processes. Interestingly, The IDA/IDL-HAE/HSL2 signalling system shows similarities to that of CLV3/CLE-CLV1/CLV2 involved in meristem maintenance. We propose that new peptide-ligand/receptor pairs may be identified by a hypothesis-driven approach, and the aid of genetics, as well as new methods to identify active peptides and direct peptide-RLK interactions.
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Aalen, Reidunn B.; Stenvik, Grethe-Elisabeth; Shi, Chunlin & Butenko, Melinka Alonso
(2009).
HAE and HSL receptors in cell separation events.
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Aalen, Reidunn B.; Stenvik, Grethe-Elisabeth; Shi, Chunlin & Butenko, Melinka Alonso
(2009).
Peptide ligands and receptors involved in control of cell-separation processes in Arabidopsis.
Show summary
Cell separation events take place throughout the life cycle of plants, and facilitate organ loss, root cap sloughing, dehiscence, fruit and seed shedding. After pollination has taken place in Arabidopsis, floral organs abscise. INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) that encodes a protein of 77 amino acids (aa) including an N-terminal export signal, is absolutely required for this process. Genetic evidence suggests that IDA is a small peptide ligand interacting with the receptor-like kinases (RLKs) HEASA and HEASA-LIKE2. Thus this is one of the few plant ligand/receptor pairs identified to date [3]. Genes encoding IDA-LIKE proteins, that share a common C-terminal 12aa motif (PIP), are present both in Arabidopsis and other plant species. The active IDA peptide resided within an extended PIP motif (EPIP). IDA-LIKE (IDL) proteins can in part substitute for IDA function, however, the IDL expression patterns indicate involvement in regulation of various other cell separation events, e.g. root cap sloughing and seed dispersal. Plants constitutively overexpressing IDA exhibit earlier abscission of floral organs and ectopic abscission of flowers, branches and cauline leaves and overexpression of the IDL proteins mimic this phenotype. The over-expression phenotype is dependent on functional HAE and HSL2 RLKs. We suggest that the IDL peptides interact with HEASA-LIKE receptors to control cell-separation processes, and that new peptide-ligand/receptor pairs may be identified by a hypothesis-driven approach with the aid of genetics and new methods to identify active peptides and direct peptide-RLK interactions.
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Stenvik, Grethe-Elisabeth; Tandstad, Nora Martinussen; Butenko, Melinka Alonso & Aalen, Reidunn B.
(2007).
INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)AND IDA-LIKE (IDL) PEPTIDES AND THEIR ROLES IN CELL SEPARATION PROCESSES.
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Stenvik, Grethe-Elisabeth; Tandstad, Nora Martinussen; Holmgren, Asbjørn; Kristiansen, Wenche; Butenko, Melinka Alonso & Aalen, Reidunn B.
(2007).
INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) AND IDA-LIKE (IDL) PEPTIDES AND THEIR ROLES IN PLANT DEVELOPMENT.
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Thorstensen, Tage; Grini, Paul Eivind; Alm, Vibeke; Butenko, Melinka Alonso; Rosenhave, E. Maryann & Aalen, Reidunn
(2004).
Identification of new gene families and their function using bioinformatics tools and reverse genetics.
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Butenko, Melinka Alonso; Patterson, Sara E.; Grini, Paul Eivind; Stenvik, Grethe-Elisabeth; Amundsen, Svanstrøm Silja & Mandal, Abul
[Show all 7 contributors for this article]
(2003).
IDA controls floral organ abscission in Arabidopsis, and identifies a novel family of putative ligands in plants.
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Butenko, Melinka Alonso; Patterson, Sara E.; Stenvik, Grethe-Elisabeth; Grini, Paul Eivind; Thorstensen, Tage & Mandal, Abul
[Show all 7 contributors for this article]
(2003).
The IDA gene, controlling floral organ abscission in Arabidopsis, identifies a novel family of putative ligands in plants.
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Thorstensen, Tage; Butenko, Melinka Alonso; Meza, Trine Johansen; Johnsen, Sylvia; Davies, William & Aalen, Reidunn
(2001).
Characterization of two SET-domain encoding Arabidopsis genes with similarity to Drosophila SUPPRESSOR OF VARIEGATION (3-9).
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Mercy, Inderjit Singh; Meza, Trine Johansen; Skårn, Magne; Butenko, Melinka Alonso; Håkelien, Anne-Mari & Halsekås, Camilla
[Show all 9 contributors for this article]
(2001).
). Molecluar studies of single-copy silencing lines.
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Butenko, Melinka Alonso; Mandal, Abul & Aalen, Reidunn
(2000).
Absence of floral abscission and meristem determinacy in the ethylene-sensitive Arabidopsis thaliana mutant inflorescence deficient in abscission (ida).
Show summary
We have identified a T-DNA transformed mutant line of Arabidopsis thaliana, named inflorescence deficient in abscission (ida), showing no floral abscission and an occasional reversion of floral meristems to inflorescence meristems. Backcrosses and segregation analysis showed that the mutant phenotype is inherited as a single-locus recessive trait. Although abscission zone (AZ) cells develop in the ida mutant, vivid, turgid floral organs showing no signs of senescence are found attached to full-grown, green siliques. The petals, sepals and stamens dry out completely and still remain attached to the dry septum after the shedding of mature seeds. ida seedlings feature the typical triple response of ethylene-sensitive plants indicating that the IDA gene is involved in an ethylene-independent developmental pathway controlling floral abscission, and that the ida mutant is different from both etr1 and ein1mutants.
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Aalen, Reidunn; Butenko, Melinka Alonso; Stacy, Robin Anne Prentice & Mandal, Abul
(2000).
Genetic characterization of an Arabidopsis thaliana mutant deficient in floral abscission.
Show summary
We have identified a T-DNA transformed mutant line of Arabidopsis thaliana, named inflorescence deficient in abscission (ida), showing no floral abscission and an occasional reversion of floral meristems to inflorescence meristems. Backcrosses and segregation analysis showed that the mutant phenotype is inherited as a single-locus recessive trait. PCR amplification of the nptII-marker gene in the T-DNA segregants strongly indicates that the mutation is caused by the T-DNA insertion. Similar late abscising phenotypes have previously been described for ethylene insensitive mutants, where flowers are retained longer and tend to become senescent before being shed. However, ida seeds germinated on medium containing ACC, gave rise to seedling featuring the typical triple response of ethylene sensitive plants.
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Butenko, Melinka Alonso; Aalen, Reidunn; Stacy, Robin Anne Prentice & Campillo, Elena del
(1999).
Differential expression of two distinct classes of Endo-1,4-b-D glucanases (cellulases) genes in Arabidopsis.
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Riiser, Even Sannes; Aalen, Reidunn B. & Butenko, Melinka Alonso
(2009).
Ligand-receptor matchmaking: The IDA-HAE/HSL2 signaling system and the IDL and HSL relatives.
Universitetet i Oslo.
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Butenko, Melinka Alonso & Aalen, Reidunn B.
(2006).
A genetic and molecular study of floral organ abscission in Arabidopsis thaliana using the mutant inflorescence deficient in abscission.
Unipub forlag.
