Linda Hofstad Haugen

• Skjeldal, Frode Miltzow; Haugen, Linda Hofstad; Nunes De C Mateus, Duarte; Frei, Dominik Michael; Rødseth, Anna Vik & Hu, Xian [Show all 7 contributors for this article] (2021). De novo formation of early endosomes during Rab5-to-Rab7a transition. Journal of Cell Science. ISSN 0021-9533. 134(8). doi: 10.1242/jcs.254185. Full text in Research Archive Show summary

  Rab5 and Rab7a are the main determinants of early and late endosomes and are important regulators of endosomal progression. The transport from early endosomes to late endosome seems to be regulated through an endosomal maturation switch, where Rab5 is gradually exchanged by Rab7a on the same endosome. Here, we provide new insight into the mechanism of endosomal maturation, for which we have discovered a stepwise Rab5 detachment, sequentially regulated by Rab7a. The initial detachment of Rab5 is Rab7a independent and demonstrates a diffusion-like first-phase exchange between the cytosol and the endosomal membrane, and a second phase, in which Rab5 converges into specific domains that detach as a Rab5 indigenous endosome. Consequently, we show that early endosomal maturation regulated through the Rab5-to-Rab7a switch induces the formation of new fully functional Rab5-positive early endosomes. Progression through stepwise early endosomal maturation regulates the direction of transport and, concomitantly, the homeostasis of early endosomes.

• Guadagno, Noemi Antonella; Margiotta, Azzurra; Bjørnestad, Synne Arstad; Haugen, Linda Hofstad; Kjos, Ingrid & Xiaochun, Xu [Show all 10 contributors for this article] (2020). Rab18 regulates focal adhesion dynamics by interacting with kinectin-1 at the endoplasmic reticulum . Journal of Cell Biology. ISSN 0021-9525. 219(7), p. 1–23. doi: 10.1083/JCB.201809020. Full text in Research Archive Show summary

  The members of the Rab family of small GTPases are molecular switches that regulate distinct steps in different membrane traffic pathways. In addition to this canonical function, Rabs can play a role in other processes, such as cell adhesion and motility. Here, we reveal the role of the small GTPase Rab18 as a positive regulator of directional migration in chemotaxis, and the underlying mechanism. We show that knockdown of Rab18 reduces the size of focal adhesions (FAs) and influences their dynamics. Furthermore, we found that Rab18, by directly interacting with the endoplasmic reticulum (ER)-resident protein kinectin-1, controls the anterograde kinesin-1–dependent transport of the ER required for the maturation of nascent FAs and protrusion orientation toward a chemoattractant. Altogether, our data support a model in which Rab18 regulates kinectin-1 transport toward the cell surface to form ER–FA contacts, thus promoting FA growth and cell migration during chemotaxis

• Mousavi, Seyed Ali; Skjeldal, Frode Miltzow; Fønhus, Marita Sporstøl; Haugen, Linda Hofstad; Eskild, Winnie & Berg, Trond [Show all 7 contributors for this article] (2019). Receptor-mediated endocytosis of VEGF-A in rat liver sinusoidal endothelial cells. BioMed Research International. ISSN 2314-6133. 2019, p. 1–12. doi: 10.1155/2019/5496197. Full text in Research Archive
• Distefano, Marita Borg; Haugen, Linda Hofstad; Wang, Yan; Perdreau-Dahl, Harmonie; Kjos, Ingrid & Jia, Da [Show all 10 contributors for this article] (2018). TBC1D5 controls the GTPase cycle of Rab7b. Journal of Cell Science. ISSN 0021-9533. 131(17), p. 1–13. doi: 10.1242/jcs.216630. Full text in Research Archive
• Haugen, Linda Hofstad; Skjeldal, Frode Miltzow; Bergeland, Trygve & Bakke, Oddmund (2017). Endosomal binding kinetics of Eps15 and Hrs specifically regulate the degradation of RTKs. Scientific Reports. ISSN 2045-2322. 7:17962, p. 1–13. doi: 10.1038/s41598-017-17320-2. Full text in Research Archive
• Dukic, Aleksandra; Haugen, Linda Hofstad; Pidoux, Guillaume; Leithe, Edward; Bakke, Oddmund & Tasken, Kjetil (2017). A protein kinase A-ezrin complex regulates connexin 43 gap junction communication in liver epithelial cells. Cellular Signalling. ISSN 0898-6568. 32, p. 1–11. doi: 10.1016/j.cellsig.2017.01.008. Full text in Research Archive
• Bergeland, Trygve; Haugen, Linda; Landsverk, Ole J.B.; Stenmark, Harald & Bakke, Oddmund (2008). Cell-cycle-dependent binding kinetics for the early endosomal tethering factor EEA1. EMBO Reports. ISSN 1469-221X. 9(2), p. 171–178. doi: 10.1038/sj.embor.7401152.

View all works in Cristin