Inger Sandlie

My research group has studied structure and function of antibodies and T-cell receptors and engineered these specific immune system detectors for use in therapy and as research tools. I am particularly interested in the interaction of both molecules with their specific receptors. Furthermore, my research group unraveled how albumin and IgG share an intracellular receptor, FcRn, that regulates the serum half-life and biodistribution of both.
I received my PhD from the University of Bergen in 1981 in the field of DNA and nucleotide synthesis. I then held postdoctoral positions at Johns Hopkins University and the Radium Hospital in Oslo, where I started my studies of antibody structure and function. I joined UiO in 1988, first as assistant professor in Molecular Biology and then, in 1995, as full professor. From 2007-2017 I was deputy director of the Centre for Immune Regulation, a Centre of Excellence appointed by the Research Council of Norway and held a position as senior researcher at Oslo University Hospital (OUH). 2017-2020, I was deputy director of a Federation of Clinical Immunology Societies Centre of Excellence, consisting of 18 research groups located in Oslo, and president of the Norwegian Society for Immunology.
I have co-authored more than 130 publications since 1988, and have received awards for scientific innovation. I am co-inventor on a number of patents describing antibody variants and ”Vaccibodies”, ”Phagemers” and the ”Veltis®” technology and co-founder of two biotechnology companies: Vaccibody A/S and Nextera A/S. I have consulted for Vaccibody A/S (Norway), Albumedix A/S (UK) and for Syntimmune A/S (USA), and presently serve on the board of the technology transfer office of UiO and OUH (Inven2 A/S) and Radforsk. I am member of the Advisory Board for OUH Comprehensive Cancer Centre and Lund University’s board for research evaluation. Furthermore, I have served on boards at the Norwegian Research Council, consulted for the Swedish and Finnish research councils and been member of the Norwegian Biotechnology Advisory Board.
I was elected member of the Norwegian Academy of Science and Letters in 2002, and served as group leader (Cell Biology) from 2011-2019.
I have supervised more than 60 MSc students and 20 PhD students, and have evaluated doctoral degrees in Norway, Sweden, Switzerland and Australia. I was on a 3 months sabbatical at Brigham and Womens’s Hospital and Harvard Medical School, Boston in 2015, and at the Scripps Research Institute, La Jolla in 2016.
Contributions to Science the last 10 years
Immunoreceptor engineering:
We have established a novel phage display platform, socalled Signal Sequence Independent phage display – SSIp display. The key finding is that two viral capsid proteins, pVII and pIX, display fusion proteins extremely well, and they do so without the need for translational signal sequences (Nilssen NR et al, Nucleic Acids Res 2012 and Løset GÅ et al, PLoS One 2011). Importantly, we have found that the SSIp display phage particles can display not only antibody fragments and T cell receptors (Høydahl LS et al, Sci Reports 2016 and Gunnarsen KS et al, Sci Reports 2013), but also MHC class II peptide complexes, and we call the MHC class II displaying phages “Phagemers”. Phagemers bind to specific T cell receptors, and thus detect T cells based on this specificity. We have explored the use of Phagemers as diagnostic tools. SSIp display is the core technology of the spin-out company Nextera A/S (www.nextera.no). Nextera A/S commercializes and develops the Phagemer technology and selects binders from large phagemer libraries, the aim being target discovery for drug development.
Studies of celiac disease:
Celiac disease (CD) develops in individuals with certain MHC class II types, and in particular, DQ2.5, which is characterized by its ability to bind and present modified gluten peptides on the surface of antigen presenting cells. To answer questions regarding the nature of these antigen presenting cells in the gut of CD patients, we designed antibodies that bind gluten-DQ2.5 complexes with very high specificity, using SSIp display. In collaboration with Ludvig Sollid and Frode Jahnsens groups, we then found the antibodies to bind B cells and plasma cells in patient biopsies. We hypothesize that the interaction between these cells and disease-associated T cells is a key driver of disease, as the detecting antibodies also inhibit T cell activation and are drug candidates. CD patients produce autoantibodies against transglutaminase 2, the enzyme that modifies the gluten peptides. Interestingly, at least some of the gluten-DQ2.5 presenting plasma cells are transglutaminase 2 specific (Høydahl LS et al, Gastroenterology 2019 and Frick R et al, Science Immunology 2021).
The T cell response against gluten-DQ2.5 complexes is biased in CD patients, in that their T cell receptors are often composed of signature variable segment pairs selected for being particularly well suited for complex binding. In collaboration with Ludvig Sollid and Shuo Wang Qiao, we have unraveled the molecular basis for a common signature, and identified an MHC recognition motif centered on a certain amino acid residue in the T cell receptor. This one residue not only contacts MHC, but also directs a pivotal part of the T cell receptor to contact the gluten peptide (Gunnarsen KS et al, JCI Insight 2017).
Half-life of IgG antibodies and albumin fused drugs:
Most proteins in blood degrade within a few hours or days, but IgG and albumin, which are the two most abundant, are rescued from degradation and have half-lives of three weeks. This is due to the fact that they bind the neonatal Fc receptor (FcRn), and we study how FcRn binding regulates the serum half-life and biodistribution of both ligands.
The long half-life is important for the therapeutic success of IgG antibodies, and thus, there is an intense interest in increasing the strength of the FcRn interaction to prolong the half-life even further. The FcRn interaction is pH dependent, with histidine dependent strong binding at acidic pH and little or no binding at neutral pH. We have studied how FcRn binds IgG molecules of different subclasses, and found that members of the IgG3 subclass degrade faster than the others because of a slight alteration in FcRn binding kinetics (Stapleton NM et al, Nat Commun 2011). This is due to a single amino acid being arginine in IgG3 and histidine in the other subclasses. We have also engineered an IgG variant with increased binding at acidic pH, which has enhanced half-life as well as ability to activate complement (PCT/IB2017/000327). The patent was recently licensed by a large drug development company.
We have also unraveled the interaction between FcRn and albumin (Andersen JT et al, Nat Commun 2012). Again, it is pH and histidine dependent as well as largely hydrophobic in nature (Sand KMK et al, J Biol Chem 2014). We have designed albumin variants with increased binding to FcRn at acidic pH that have increased half-life in rodents and monkeys (Andersen JT et al, J Biol Chem 2014 and described in several patents). Our invention, The Veltis ®technology (albumin attachment to drugs) is commercialized by Albumedix A/S, a collaborating drug development company, and involves both normal albumin and our engineered albumin variants.
Biodistribution of IgG antibodies
FcRn is expressed intracellularly, and binds IgG taken up by fluid-phase endocytosis. It can then direct monomeric IgG to the surface of the opposite side of the cell (transcytosis) or to the side of entry (recycling). We have studied both processes using the natural ligands as well as engineered variants (Foss S et al, J Control Release 2016).
A new in vitro recycling assay designed by us predicts the behavior of designed FcRn-binding molecules in vivo in animal models (Grevys A et al, Nat Commun 2017). We have found that FcRn is the only Fc receptor required for transport of IgG across cellular barriers and placenta (Mathiesen et al, Blood 2013).
In collaboration with Richard Blumberg’s laboratory, we have found that it enhances antigen presentation and cross presentation of antigen in immune complexes by specialized dendritic cells (Baker K et al, Proc Natl Acad Sci 2011). Furthermore, we recently found that FcRn cooperates with FcγRIIa to regulate innate cytokine production and antigen presentation through formation of a ternary complex consisting of FcRn, FcγRIIa and IgG IC (Hubbard J et al, J Exp Med 2020).
Biodistribution of albumin and albumin fused drugs
In collaboration with Richard Blumberg’s laboratory, we found that albumin produced in the liver is released to the bloodstream, and directed away from the bile (Pyzik M et al, Proc Natl Acad Sci 2017).
Importantly, we recently found that albumin is transcytosed efficiently from the apical to the basolateral side of the mucosae, and think this observation holds great promise for mucosal delivery of albumin based vaccines and therapeutics (Bern M et al, J Control Release 2015 and Science Translational Med 2020).
Previous positions
2017-2020, Deputy Director of a Federation of Clinical Immunology Societies Centre of Excellence
2007- 2017 Deputy Director of Centre for Immune Regulation, UiO
1995- 2020 Professor of Molecular Biology, Department of Biosciences, UiO
1988-1995 Assistant professor, Department of Biology, UiO
1985-1988 Researcher, Norwegian Radium Hospital, Oslo
1982-1984 Postdoc, Department of Biochemistry, Johns Hopkins University, MD, USA
Honors and Memberships
2016- Honorary member of the Norwegian Society for Immunology
2015- Innovation prize from Inven2
2011- First Innovation prize awarded by UiO
2007- Member of Henry Kunkel Society, Rockefeller University, USA
2003- Innovation prize from Medinnova for “Vaccibodies”
2002- Elected member of the Norwegian Academy of Science and Letters. Group leader (Cell Biology) 2011-2019
1996- Innovation prize for “Troybodies”
Other Experience
2017-2020 President of the Norwegian Society for Immunology
2014- 2019 Scientific Advisory Board member: Syntimmune A/S, Vaccibody A/S, Albumedix A/S
2010- present Board member Inven2 A/S and Radforsk
2006- 2020 Reviewer Swedish and Finnish Research Councils
2004- 2020 PhD thesis opponent in Norway, Sweden, Denmark, Switzerland and Australia
1998- 2000 Member of the Norwegian Biotechnology Advisory Board
1995- Member Advisory Boards at The Norwegian Research Council
Patents and industrialisation
Coinventor on 16 patents granted or pending on modified antibodies and albumin variants. Cofounder of Vaccibody A/S (https://www.vaccibody.com) and Nextera A/S (https://nextera.no).
Publications
-
Grevys, Algirdas; Frick, Rahel; Mester, Simone; Flem-Karlsen, Karine; Nilsen, Jeannette & Foss, Stian [Show all 13 contributors for this article] (2022). Antibody variable sequences have a pronounced effect on cellular transport and plasma half-life. iScience. ISSN 2589-0042. 25(2). doi: 10.1016/j.isci.2022.103746.
-
Frick, Rahel; Høydahl, Lene Støkken; Petersen, Jan; du Pré, Fleur; Kumari, Shraddha & Berntsen, Grete [Show all 20 contributors for this article] (2021). A high-affinity human TCR-like antibody detects celiac disease gluten peptide-MHC complexes and inhibits T cell activation. Science immunology. ISSN 2470-9468. 6:eabg4925(62), p. 1–16. doi: 10.1126/sciimmunol.abg4925. Full text in Research Archive
-
Mester, Simone; Evers, Mitchell; Meyer, Saskia; Nilsen, Jeannette; Greiff, Victor & Sandlie, Inger [Show all 8 contributors for this article] (2021). Extended plasma half-life of albumin-binding domain fused human IgA upon pH-dependent albumin engagement of human FcRn in vitro and in vivo. mAbs. ISSN 1942-0862. 13:1893888(1), p. 1–14. doi: 10.1080/19420862.2021.1893888. Full text in Research Archive
-
Høydahl, Lene; Frigstad, Terje; Rasmussen, Ingunn B; Øynebråten, Inger; Schjetne, Karoline W & Andersen, Jan Terje [Show all 10 contributors for this article] (2021). Antibody-mediated delivery of T-cell epitopes to antigen-presenting cells induce strong CD4 and CD8 T-cell responses. Vaccine. ISSN 0264-410X. 39(11), p. 1583–1592. doi: 10.1016/j.vaccine.2021.02.012. Full text in Research Archive
-
Hovden Aaen, Kristin; Anthi, Aina Karen; Sandlie, Inger; Nilsen, Jeannette; Mester, Simone & Andersen, Jan Terje (2020). The neonatal Fc receptor in mucosal immune regulation. Scandinavian Journal of Immunology. ISSN 0300-9475. doi: 10.1111/sji.13017.
-
Nilsen, Jeannette; Trabjerg, Esben; Grevys, Algirdas; Azevedo, Claudia; Brennan, Stephen O. & Stensland, Maria [Show all 14 contributors for this article] (2020). An intact C-terminal end of albumin is required for its long half-life in humans. Communications Biology. ISSN 2399-3642. 3(1). doi: 10.1038/s42003-020-0903-7. Full text in Research Archive
-
Hubbard, Jonathan J.; Pyzik, Michal; Rath, Timo; Kozicky, Lisa K.; Sand, Kine Marita Knudsen & Gandhi, Amit K. [Show all 17 contributors for this article] (2020). FcRn is a CD32a coreceptor that determines susceptibility to IgG immune complex-driven autoimmunity. Journal of Experimental Medicine (JEM). ISSN 0022-1007. 217(10). doi: 10.1084/jem.20200359.
-
Bern, Malin C.; Nilsen, Jeannette; Ferrarese, Mattia; Sand, Kine Marita Knudsen; Tollefsrud Gjølberg, Torleif & Lode, Heidrun Elisabeth [Show all 24 contributors for this article] (2020). An engineered human albumin enhances half-life and transmucosal delivery when fused to protein-based biologics. Science Translational Medicine. ISSN 1946-6234. 12(565), p. 1–13. doi: 10.1126/scitranslmed.abb0580. Full text in Research Archive
-
Frick, Rahel; Gunnarsen, Kristin Støen; Dahal-Koirala, Shiva; Risnes, Louise Fremgaard; Sollid, Ludvig Magne & Sandlie, Inger [Show all 8 contributors for this article] (2019). A TRAV26-1-encoded recognition motif focuses the biased T cell response in celiac disease. European Journal of Immunology. ISSN 0014-2980. 50, p. 142–145. doi: 10.1002/eji.201948235. Full text in Research Archive
-
Høydahl, Lene Støkken; Frick, Rahel; Sandlie, Inger & Løset, Geir Åge (2019). Targeting the MHC Ligandome by Use of TCR-Like Antibodies. Antibodies. ISSN 2073-4468. 8(32), p. 1–21. doi: 10.3390/antib8020032. Full text in Research Archive
-
Foss, Stian; Bottermann, Maria; Jonsson, Alexandra; Sandlie, Inger; James, Leo C. & Andersen, Jan Terje (2019). TRIM21-From intracellular immunity to therapy. Frontiers in Immunology. ISSN 1664-3224. 10:2049, p. 1–14. doi: 10.3389/fimmu.2019.02049. Full text in Research Archive
-
Bottermann, Maria; Foss, Stian; Caddy, Sarah L.; Clift, Dean; van Tienen, Laurens M. & Vaysburd, Marina [Show all 16 contributors for this article] (2019). Complement C4 prevents viral infection through capsid inactivation. Cell Host and Microbe. ISSN 1931-3128. 25(4), p. 617–629. doi: 10.1016/j.chom.2019.02.016. Full text in Research Archive
-
Pyzik, Michal; Sand, Kine Marita Knudsen; Hubbard, Jonathan J.; Andersen, Jan Terje; Sandlie, Inger & Blumberg, Richard S. (2019). The neonatal Fc Receptor (FcRn): A misnomer? Frontiers in Immunology. ISSN 1664-3224. 10:1540(JULY), p. 1–24. doi: 10.3389/fimmu.2019.01540. Full text in Research Archive
-
Shaw, Alan; Hoffecker, Ian T.; Smyrlaki, Ioanna; Rosa, João; Grevys, Algirdas & Bryant-Bratlie, Diane Lynn [Show all 10 contributors for this article] (2019). Binding to nanopatterned antigens is dominated by the spatial tolerance of antibodies. Nature Nanotechnology. ISSN 1748-3387. 14(2), p. 184–190. doi: 10.1038/s41565-018-0336-3.
-
Høydahl, Lene Støkken; Richter, Lisa Maria; Frick, Rahel; Snir, Omri; Gunnarsen, Kristin Støen & Landsverk, Ole J. B. [Show all 18 contributors for this article] (2019). Plasma Cells Are the Most Abundant Gluten Peptide MHC-expressing Cells in Inflamed Intestinal Tissues From Patients With Celiac Disease. Gastroenterology. ISSN 0016-5085. 156(5), p. 1428–1439.e10. doi: 10.1053/j.gastro.2018.12.013. Full text in Research Archive
-
Nilsen, Jeannette; Bern, Malin C.; Sand, Kine Marita Knudsen; Grevys, Algirdas; Dalhus, Bjørn & Sandlie, Inger [Show all 7 contributors for this article] (2018). Human and mouse albumin bind their respective neonatal Fc receptors differently. Scientific Reports. ISSN 2045-2322. 8:14648, p. 1–12. doi: 10.1038/s41598-018-32817-0. Full text in Research Archive
-
Grevys, Algirdas; Nilsen, Jeannette; Sand, Kine Marita Knudsen; Daba, Muluneh Bekele; Øynebråten, Inger & Bern, Malin C. [Show all 15 contributors for this article] (2018). A human endothelial cell-based recycling assay for screening of FcRn targeted molecules. Nature Communications. ISSN 2041-1723. 9. doi: 10.1038/s41467-018-03061-x. Full text in Research Archive
-
Nilsen, Jeannette; Sandlie, Inger; Roopenian, Derry C. & Andersen, Jan Terje (2018). Animal models for evaluation of albumin-based therapeutics. Current Opinion in Chemical Engineering. ISSN 2211-3398. 19, p. 68–76. doi: 10.1016/j.coche.2017.11.007. Full text in Research Archive
-
Gunnarsen, Kristin Støen; Høydahl, Lene Støkken; Neumann, Ralf Stefan; Bjerregaard-Andersen, Kaare; Nilssen, Nicolay Rustad & Sollid, Ludvig Magne [Show all 8 contributors for this article] (2018). Soluble T-cell receptor design influences functional yield in an E. coli chaperone-assisted expression system. PLOS ONE. ISSN 1932-6203. 13:e0195868(4), p. 1–15. doi: 10.1371/journal.pone.0195868. Full text in Research Archive Show summary
-
Pyzik, Michal; Rath, Timo; Kuo, Timothy T.; Win, Sanda; Baker, Kristi & Hubbard, Jonathan J. [Show all 24 contributors for this article] (2017). Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 114(14), p. E2862–E2871. doi: 10.1073/pnas.1618291114. Full text in Research Archive
-
Bottermann, Maria; Lode, Heidrun Elisabeth; Watkinson, Ruth E.; Foss, Stian; Sandlie, Inger & Andersen, Jan Terje [Show all 7 contributors for this article] (2017). Corrigendum: Antibody-antigen kinetics constrain intracellular humoral immunity. Scientific Reports. ISSN 2045-2322. doi: 10.1038/srep45418. Full text in Research Archive
-
Gunnarsen, Kristin Støen; Høydahl, Lene Støkken; Risnes, Louise Fremgaard; Dahal-Koirala, Shiva; Neumann, Ralf Stefan & Bergseng, Elin [Show all 15 contributors for this article] (2017). A TCRα framework–centered codon shapes a biased T cell repertoire through direct MHC and CDR3β interactions. JCI Insight. ISSN 2379-3708. 2(17). doi: 10.1172/jci.insight.95193. Full text in Research Archive
-
Michaelsen, Terje Einar; Emilsen, Sidsel; Sandin, Randi Helene; Granerud, Beathe Kiland; Bratlie, Diane Lynn Bryant & Ihle, Øistein [Show all 7 contributors for this article] (2017). Human secretory IgM antibodies activate human complement and offer protection at mucosal surface. Scandinavian Journal of Immunology. ISSN 0300-9475. 83(1), p. 43–50. doi: 10.1111/sji.12508. Full text in Research Archive
-
Høydahl, Lene Støkken; Nilssen, Nicolay Rustad; Gunnarsen, Kristin Støen; du Pré, Marie Fleur; Iversen, Rasmus & Roos, Norbert [Show all 11 contributors for this article] (2016). Multivalent pIX phage display selects for distinct and improved antibody properties. Scientific Reports. ISSN 2045-2322. 6. doi: 10.1038/srep39066. Full text in Research Archive
-
Bottermann, Maria; Lode, Heidrun Elisabeth; Watkinson, Ruth E; Foss, Stian; Sandlie, Inger & Andersen, Jan Terje [Show all 7 contributors for this article] (2016). Antibody-antigen kinetics constrain intracellular humoral immunity. Scientific Reports. ISSN 2045-2322. 6. doi: 10.1038/srep37457. Full text in Research Archive
-
Foss, Stian; Watkinson, Ruth E; Grevys, Algirdas; Mc, Adam Martin Berner; Bern, Malin C. & Høydahl, Lene Støkken [Show all 11 contributors for this article] (2016). TRIM21 Immune Signaling Is More Sensitive to Antibody Affinity Than Its Neutralization Activity. Journal of Immunology. ISSN 0022-1767. 196(8), p. 3452–3459. doi: 10.4049/jimmunol.1502601. Full text in Research Archive
-
Foss, Stian; Grevys, Algirdas; Sand, Kine Marita Knudsen; Bern, Malin C.; Blundell, Pat & Michaelsen, Terje Einar [Show all 9 contributors for this article] (2016). Enhanced FcRn-dependent transepithelial delivery of IgG by Fc-engineering and polymerization. Journal of Controlled Release. ISSN 0168-3659. 223, p. 42–52. doi: 10.1016/j.jconrel.2015.12.033. Full text in Research Archive
-
Foss, Stian; Watkinson, Ruth; Sandlie, Inger; James, Leo C. & Andersen, Jan Terje (2015). TRIM21: A cytosolic Fc receptor with broad antibody isotype specificity. Immunological Reviews. ISSN 0105-2896. 268(1), p. 328–339. doi: 10.1111/imr.12363. Full text in Research Archive
-
Løset, Geir Åge; Berntzen, Gøril; Frigstad, Terje; Pollmann, Sylvie; Gunnarsen, Kristin Støen & Sandlie, Inger (2015). Phage display engineered T cell receptors as tools for the study of tumor peptide–MHC interactions. Frontiers in Oncology. ISSN 2234-943X. doi: 10.3389/fonc.2014.00378. Full text in Research Archive
-
Grevys, Algirdas; Bern, Malin C.; Foss, Stian; Bratlie, Diane Lynn Bryant; Moen, Anders & Gunnarsen, Kristin Støen [Show all 10 contributors for this article] (2015). Fc Engineering of Human IgG1 for Altered Binding to the Neonatal Fc Receptor Affects Fc Effector Functions. Journal of Immunology. ISSN 0022-1767. 194(11), p. 5497–5508. doi: 10.4049/jimmunol.1401218. Full text in Research Archive
-
Daniel M, Czajkowsky; Andersen, Jan Terje; Fuchs, Anja; Wilson, Timothy J; Mekhaiel, David & Colonna, Marco [Show all 17 contributors for this article] (2015). Developing the IVIG biomimetic, Hexa-Fc, for drug and vaccine applications. Scientific Reports. ISSN 2045-2322. 5, p. 1–11. doi: 10.1038/srep09526. Full text in Research Archive
-
Bern, Malin C.; Sand, Kine Marita Knudsen; Nilsen, Jeannette; Sandlie, Inger & Andersen, Jan Terje (2015). The role of albumin receptors in regulation of albumin homeostasis: Implications for drug delivery. Journal of Controlled Release. ISSN 0168-3659. 211, p. 144–162. doi: 10.1016/j.jconrel.2015.06.006.
-
Sand, Kine Marita Knudsen; Bern, Malin C.; Nilsen, Jeannette; Noordzij, Hanna Theodora; Sandlie, Inger & Andersen, Jan Terje (2015). Unraveling the interaction between FcRn and albumin: Opportunities for design of albumin-based therapeutics. Frontiers in Immunology. ISSN 1664-3224. 5:682. doi: 10.3389/fimmu.2014.00682. Full text in Research Archive
-
Hallstensen, Randi; Bergseth, Grete; Foss, Stian; Jæger, Steinar; Gedde-Dahl, Thobias & Holt, jan [Show all 14 contributors for this article] (2015). Eculizumab treatment during pregnancy does not affect the complement system activity of the newborn. Immunobiology. ISSN 0171-2985. 220(4), p. 452–459. doi: 10.1016/j.imbio.2014.11.003. Full text in Research Archive
-
Andersen, Jan Terje; Hjelstuen, Ole Kristian & Sandlie, Inger (2014). From Basic Research to Innovation, Medical Technology - Meeting Tomorrow's Health Care Challenges. Novus Forlag. ISSN 978-82-7099-791-6. p. 39–50.
-
Sand, Kine Marita Knudsen; Dalhus, Bjørn; Christianson, Gregory J.; Bern, Malin C.; Foss, Stian & Cameron, Jason [Show all 11 contributors for this article] (2014). Dissection of the neonatal Fc receptor (FcRn)-albumin interface using mutagenesis and anti-FcRn albumin-blocking antibodies. Journal of Biological Chemistry. ISSN 0021-9258. doi: 10.1074/jbc.M113.522565.
-
Sand, Kine Marita Knudsen; Bern, Malin C.; Nilsen, Jeannette; Dalhus, Bjørn; Gunnarsen, Kristin Støen & Cameron, Jason [Show all 10 contributors for this article] (2014). Interaction with both domain I and III of albumin is required for optimal pH-dependent binding to the neonatal Fc Receptor (FcRn). Journal of Biological Chemistry. ISSN 0021-9258. 289(50), p. 34583–34594. doi: 10.1074/jbc.M114.587675. Full text in Research Archive
-
Andersen, Jan Terje; Dalhus, Bjørn; Viuff, Dorthe; Ravn, Birgitte Thue; Gunnarsen, Kristin Støen & Plumridge, Andrew [Show all 17 contributors for this article] (2014). Extending serum half-life of albumin by engineering neonatal Fc receptor (FcRn) binding. Journal of Biological Chemistry. ISSN 0021-9258. 289(19), p. 13492–13502. doi: 10.1074/jbc.M114.549832. Full text in Research Archive
-
Sand, Kine Marita Knudsen; Bern, Malin C.; Nilsen, Jeannette; Dalhus, Bjørn; Gunnarsen, Kristin Støen & Cameron, Jason [Show all 10 contributors for this article] (2014). Interaction with both domain I and III of albumin is required for optimal pH-dependent binding to the neonatal Fc Receptor (FcRn). Journal of Biological Chemistry. ISSN 0021-9258. 289(50), p. 34583–34594. doi: 10.1074/jbc.M114.587675.
-
Sand, Kine Marita Knudsen; Dalhus, Bjørn; Christianson, Gregory J.; Bern, Malin C.; Foss, Stian & Cameron, Jason [Show all 11 contributors for this article] (2014). Dissection of the neonatal Fc receptor (FcRn)-albumin interface using mutagenesis and anti-FcRn albumin-blocking antibodies. Journal of Biological Chemistry. ISSN 0021-9258. 289(24), p. 17228–17239. doi: 10.1074/jbc.M113.522565. Full text in Research Archive
-
Rahman, Mohummad Aminur; Kristiansen, Per Eugen; Veiseth, Silje Veie; Andersen, Jan Terje; Yap, Kyoko L. & Zhou, Ming-Ming [Show all 9 contributors for this article] (2014). The arabidopsis histone methyltransferase SUVR4 binds ubiquitin via a domain with a four-helix bundle structure. Biochemistry. ISSN 0006-2960. 53(13), p. 2091–2100. doi: 10.1021/bi401436h.
-
Jung, Sang Taek; Kelton, William; Kang, Tae Hyun; T.W. Ng, Daphne; Andersen, Jan Terje & Sandlie, Inger [Show all 8 contributors for this article] (2013). Effective Phagocytosis of Low Her2 Tumor Cell Lines with Engineered, Aglycosylated IgG Displaying High FcγRIIa Affinity and Selectivity. ACS Chemical Biology. ISSN 1554-8929. 8(2), p. 368–375. doi: 10.1021/cb300455f.
-
Yazaki, Paul J.; Lee, Brian; Channappal, Divya; Cheung, Chia-Wei; Crow, Desiree & Chea, Junie [Show all 15 contributors for this article] (2013). A series of anti-CEA/anti-DOTA bispecific antibody formats evaluated for pre-targeting: comparison of tumor uptake and blood clearance. Protein Engineering Design & Selection. ISSN 1741-0126. 26(3), p. 187–193. doi: 10.1093/protein/gzs096.
-
Mathiesen, Line; Nielsen, Leif K.; Andersen, Jan Terje; Grevys, Algirdas; Sandlie, Inger & Michaelsen, Terje Einar [Show all 9 contributors for this article] (2013). Maternofetal transplacental transport of recombinant IgG antibodies lacking effector functions. Blood. ISSN 0006-4971. 122(7), p. 1174–1181. doi: 10.1182/blood-2012-12-473843. Full text in Research Archive
-
Lau, Corinna; Gunnarsen, Kristin Støen; Høydahl, Lene Støkken; Andersen, Jan Terje; Berntzen, Gøril & Pharo, Anne Margrethe [Show all 15 contributors for this article] (2013). Chimeric Anti-CD14 IGG2/4 Hybrid Antibodies for Therapeutic Intervention in Pig and Human Models of Inflammation. Journal of Immunology. ISSN 0022-1767. 191(9), p. 4769–4777. doi: 10.4049/jimmunol.1301653. Full text in Research Archive
-
Andersen, Jan Terje; Cameron, J; Plumridge, A; Sleep, D; Evans, L & Sandlie, Inger (2013). Single-chain Variable Fragment Albumin Fusions Bind the Neonatal Fc Receptor (FcRn) in a Species-dependent Manner: IMPLICATIONS FOR IN VIVO HALF-LIFE EVALUATION OF ALBUMIN FUSION THERAPEUTICS. Journal of Biological Chemistry. ISSN 0021-9258. 288(33), p. 24277–24285. doi: 10.1074/jbc.M113.463000. Full text in Research Archive
-
Frigstad, Terje; Løset, Geir Åge; Sandlie, Inger & Bogen, Bjarne (2013). A Public T cell Receptor Recognized by a MonoclonalAntibody Specific for the D-J Junction of the b-chain. Scandinavian Journal of Immunology. ISSN 0300-9475. p. 345–351. doi: 10.1111/sji.12098.
-
Andersen, Jan Terje; Gonzalez-Pajuelo, Maria; Foss, Stian; Landsverk, Ole J. B.; Pinto, Debora & Szyroki, Alexander [Show all 10 contributors for this article] (2013). Selection of Nanobodies that Target Human Neonatal Fc Receptor. Scientific Reports. ISSN 2045-2322. 3. doi: 10.1038/srep01118. Full text in Research Archive
-
Gunnarsen, Kristin Støen; Kristinsson, Solveig Gunn; Justesen, Sune; Frigstad, Terje; Buus, Søren & Bogen, Bjarne [Show all 8 contributors for this article] (2013). Chaperone-assisted thermostability engineering of a soluble T cell receptor using phage display. Scientific Reports. ISSN 2045-2322. 3. doi: 10.1038/srep01162. Full text in Research Archive
-
Løset, Geir Åge & Sandlie, Inger (2012). Next generation phage display by use of pVII and pIX as display scaffolds. Methods. ISSN 1046-2023. 58(1), p. 40–46. doi: 10.1016/j.ymeth.2012.07.005.
-
Fredriksen, Agnete; Sandlie, Inger & Bogen, Bjarne (2012). Targeted DNA vaccines for enhanced induction of idiotype-specific B and T cells. Frontiers in Oncology. ISSN 2234-943X. 2. doi: 10.3389/fonc.2012.00154. Full text in Research Archive
-
Andersen, Jan Terje; Foss, Stian; Kenanova, Vania E; Olafsen, Tove; Leikfoss, Ingvild Sørum & Roopenian, Derry C [Show all 8 contributors for this article] (2012). Anti-carcinoembryonic Antigen Single-chain Variable Fragment Antibody Variants Bind Mouse and Human Neonatal Fc Receptor with Different Affinities That Reveal Distinct Cross-species Differences in Serum Half-life. Journal of Biological Chemistry. ISSN 0021-9258. 287(27), p. 22927–22937. doi: 10.1074/jbc.M112.355131. Full text in Research Archive
-
Nilssen, Nicolay Rustad; Frigstad, Terje; Pollmann, Sylvie; Roos, Norbert; Bogen, Bjarne & Sandlie, Inger [Show all 7 contributors for this article] (2012). DeltaPhage-a novel helper phage for high-valence pIX phagemid display. Nucleic Acids Research (NAR). ISSN 0305-1048. 40(16). doi: 10.1093/nar/gks341. Full text in Research Archive
-
Rasmussen, Ingunn B; Øynebråten, Inger; Høydahl, Lene Støkken; Flobakk, Morten; Lunde, Elin & Michaelsen, Terje Einar [Show all 8 contributors for this article] (2012). CD40/APC-specific antibodies with three T-cell epitopes loaded in the constant domains induce CD4 T-cell responses. Protein Engineering Design & Selection. ISSN 1741-0126. 25(3), p. 89–96. doi: 10.1093/protein/gzr063. Full text in Research Archive
-
Andersen, Jan Terje; Dalhus, Bjørn; Cameron, Jason; Daba, Muluneh Bekele; Plumridge, Andrew & Evans, Leslie [Show all 11 contributors for this article] (2012). Structure-based mutagenesis reveals the albumin-binding site of the neonatal Fc receptor. Nature Communications. ISSN 2041-1723. 3. doi: 10.1038/ncomms1607. Full text in Research Archive
-
Wälchli, Sebastien; Løset, Geir Åge; Kumari, Shraddha; Johansen, Jorunn; Yang, Weiwen & Sandlie, Inger [Show all 7 contributors for this article] (2011). A Practical Approach to T-Cell Receptor Cloning and Expression. PLOS ONE. ISSN 1932-6203. 6(11). doi: 10.1371/journal.pone.0027930. Full text in Research Archive
-
Stapleton, NM; Andersen, Jan Terje; Stemerding, AM; Bjarnarson, SP; Verheul, RC & Gerritsen, J. [Show all 13 contributors for this article] (2011). Competition for FcRn-mediated transport gives rise to short half-life of human IgG3 and offers therapeutic potential. Nature Communications. ISSN 2041-1723. 2. doi: 10.1038/ncomms1608. Full text in Research Archive Show summary
-
Mekhaiel, David N. A.; Czajkowsky, Daniel M.; Andersen, Jan Terje; Shi, Jianguo; El-Faham, Marwa & Doenhoff, Michael [Show all 12 contributors for this article] (2011). Polymeric human Fc-fusion proteins with modified effector functions. Scientific Reports. ISSN 2045-2322. 1. doi: 10.1038/srep00124. Full text in Research Archive
-
Qiao, Shuo Wang; Raki, Melinda; Gunnarsen, Kristin Støen; Løset, Geir Åge; Lundin, Knut E A & Sandlie, Inger [Show all 7 contributors for this article] (2011). Posttranslational Modification of Gluten Shapes TCR Usage in Celiac Disease. Journal of Immunology. ISSN 0022-1767. 187(6), p. 3064–3071. doi: 10.4049/jimmunol.1101526.
-
Baker, Kristi; Qiao, Shuo Wang; Kuo, Timothy T.; Aveson, Victoria G.; Platzer, Barbara & Andersen, Jan Terje [Show all 12 contributors for this article] (2011). Neonatal Fc receptor for IgG (FcRn) regulates cross-presentation of IgG immune complexes by CD8(-)CD11b(+) dendritic cells. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 108(24), p. 9927–9932. doi: 10.1073/pnas.1019037108.
-
Løset, Geir Åge; Bogen, Bjarne & Sandlie, Inger (2011). Expanding the Versatility of Phage Display I: Efficient Display of Peptide-Tags on Protein VII of the Filamentous Phage. PLOS ONE. ISSN 1932-6203. 6(2). doi: 10.1371/journal.pone.0014702. Full text in Research Archive
-
Løset, Geir Åge; Roos, Norbert; Bogen, Bjarne & Sandlie, Inger (2011). Expanding the Versatility of Phage Display II: Improved Affinity Selection of Folded Domains on Protein VII and IX of the Filamentous Phage. PLOS ONE. ISSN 1932-6203. 6(2). doi: 10.1371/journal.pone.0017433. Full text in Research Archive
-
Lunde, Elin; Løset, Geir Åge; Bogen, Bjarne & Sandlie, Inger (2010). Stabilizing mutations increase secretion of functional soluble TCR-Ig fusion proteins. BMC Biotechnology. ISSN 1472-6750. 10. doi: 10.1186/1472-6750-10-61. Full text in Research Archive
-
Andersen, Jan Terje; Daba, Muluneh Bekele & Sandlie, Inger (2010). FcRn binding properties of an abnormal truncated analbuminemic albumin variant. Clinical Biochemistry. ISSN 0009-9120. 43(4-5), p. 367–372. doi: 10.1016/j.clinbiochem.2009.12.001.
-
Gunnarsen, Kristin Støen; Lunde, Elin; Kristiansen, Per Eugen; Bogen, Bjarne; Sandlie, Inger & Løset, Geir Åge (2010). Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA. BMC Biotechnology. ISSN 1472-6750. 10. doi: 10.1186/1472-6750-10-8. Full text in Research Archive
-
Jung, Sang Taek; Reddy, Sai T.; Kang, Tae Hyun; Borrok, M. Jack; Sandlie, Inger & Tucker, Philip W. [Show all 7 contributors for this article] (2010). Aglycosylated IgG variants expressed in bacteria that selectively bind Fc gamma RI potentiate tumor cell killing by monocyte-dendritic cells. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 107(2), p. 604–609. doi: 10.1073/pnas.0908590107.
-
Andersen, Jan Terje; Daba, Muluneh Bekele; Berntzen, Gøril; Michaelsen, Terje Einar & Sandlie, Inger (2010). Cross-species binding analyses of mouse and human neonatal Fc receptor show dramatic differences in immunoglobulin G and albumin binding. Journal of Biological Chemistry. ISSN 0021-9258. 285(7), p. 4826–4836. doi: 10.1074/jbc.M109.081828. Full text in Research Archive
-
Andersen, Jan Terje & Sandlie, Inger (2009). The Versatile MHC Class I-related FcRn Protects IgG and Albumin from Degradation: Implications for Development of New Diagnostics and Therapeutics. Drug Metabolism and Pharmacokinetics (DMPK). ISSN 1347-4367. 24(4), p. 318–332.
-
Berntzen, Gøril; Andersen, Jan Terje; Ustgård, Kristine; Ustgård, Kristine; Michaelsen, Terje Einar & Mousavi, Darbi Seyed Ali [Show all 13 contributors for this article] (2009). Identification of a High Affinity Fc gamma RIIA-binding Peptide That Distinguishes Fc gamma RIIA from Fc gamma RIIB and Exploits Fc gamma RIIA-mediated Phagocytosis and Degradation. Journal of Biological Chemistry. ISSN 0021-9258. 284(2), p. 1126–1135. doi: 10.1074/jbc.M803584200. Full text in Research Archive
-
Ghumra, A; Shi, J; McIntosh, RS; Rasmussen, Ingunn B; Braathen, Ranveig & Johansen, Finn Eirik [Show all 13 contributors for this article] (2009). Structural requirements for the interaction of human IgM and IgA with the human Fc alpha/mu receptor. European Journal of Immunology. ISSN 0014-2980. 39(4), p. 1147–1156. doi: 10.1002/eji.200839184.
-
Michaelsen, Terje Einar; Sandlie, Inger; Bratlie, Diane Lynn Bryant; Sandin, Randi H & Ihle, Øistein (2009). Structural difference in the complement activation site of human IgG1 and IgG3. Scandinavian Journal of Immunology. ISSN 0300-9475. 70(6), p. 553–564. doi: 10.1111/j.1365-3083.2009.02338.x. Full text in Research Archive
-
Nielsen, Leif K; Green, Trine H; Sandlie, Inger; Michaelsen, Terje Einar & Dziegiel, Morten H (2008). In vitro assessment of recombinant, mutant immunoglobulin G anti-D devoid of hemolytic activity for treatment of ongoing hemolytic disease of the fetus and newborn. Transfusion. ISSN 0041-1132. 48(1), p. 12–19. doi: 10.1111/j.1537-2995.2007.01474.x. Full text in Research Archive
-
Andersen, Jan Terje; Justesen, Sune; Berntzen, Gøril; Michaelsen, Terje Einar; Lauvrak, Vigdis & Fleckenstein, Burkhard [Show all 8 contributors for this article] (2008). A strategy for bacterial production of a soluble functional human neonatal Fc receptor. JIM - Journal of Immunological Methods. ISSN 0022-1759. 331(1-2), p. 39–49. doi: 10.1016/j.jim.2007.11.003. Full text in Research Archive
-
Løset, Geir Åge; Kristinsson, Solveig Gunn & Sandlie, Inger (2008). Reliable titration of filamentous bacteriophages independent of pIII fusion moiety and genome size by using trypsin to restore wild-type pill phenotype. BioTechniques. ISSN 0736-6205. 44(4), p. 551–554. doi: 10.2144/000112724. Show summary
-
Andersen, Jan T; Justesen, Sune; Fleckenstein, Burkhard; Michaelsen, Terje Einar; Berntzen, Gøril & Kenanova, Vania E [Show all 10 contributors for this article] (2008). Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues. The FEBS Journal. ISSN 1742-464X. 275(16), p. 4097–4110. doi: 10.1111/j.1742-4658.2008.06551.x. Full text in Research Archive
-
Ghumra, A; Semblat, JP; McIntosh, RS; Raza, A; Rasmussen, Ingunn B & Braathen, Ranveig [Show all 11 contributors for this article] (2008). Identification of residues in the C mu 4 domain of polymeric IgM essential for interaction with Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). Journal of Immunology. ISSN 0022-1767. 181, p. 1988–2000.
-
Andersen, Jan Terje & Sandlie, Inger (2011). Pharmacokinetics of IgG and albumin: The impact of FcRn for Design of Drug Delivery Systems: The impact of FcRn for Design of Drug Delivery Systems. Wiley-VCH Verlag GmbH. ISBN 9783527328239. 34 p.
-
Andersen, Jan Terje & Sandlie, Inger (2011). Extending Antibody Half Lives with Albumin In: Recombinant Antibodies for Immunotherapy. Cambridge University Press. ISBN 9780521887328. 17 p.
-
Andersen, Jan Terje; Sandlie, Inger; Kenanova, Vania E; Olafsen, Tove & Wu, Anna M (2010). Engineering of the Fc Region for Improved PK (FcRn interaction. Springer-Verlag Berlin Heidelberg . ISBN 978-3-642-01143-6. 19 p.
-
Andersen, Jan Terje & Sandlie, Inger (2008). The neonatal Fc receptor controls immunoglobulin G and albumin homeostasis. Transworld Research Network. ISBN 8178953641. 24 p.
-
Andersen, Jan Terje; Sakya, Siri Aastedatter; Foss, Stian; Sandlie, Inger & Voets, Ardi (2019). Is an HIV vaccine within reach? Vaksinebloggen.
-
Andersen, Jan Terje; Sakya, Siri Aastedatter; Foss, Stian & Sandlie, Inger (2019). Hvordan kan moderne medisin forebygge HIV? Medisinbloggen.
-
Shaw, Alan; Hoffecker, Ian T.; Smyrlaki, Ioanna; Rosa, João; Grevys, Algirdas & Bryant-Bratlie, Diane Lynn [Show all 10 contributors for this article] (2019). Publisher Correction: Binding to nanopatterned antigens is dominated by the spatial tolerance of antibodies. Nature Nanotechnology. ISSN 1748-3387. 14(4), p. 398–398. doi: 10.1038/s41565-019-0404-3.
-
Graver, Hans Petter; Sandlie, Inger & Frich, Jan C (2017). Forvandle vår verden | Graver, Sandlie og Frich. Aftenposten (morgenutg. : trykt utg.). ISSN 0804-3116. p. 18–19.
-
Frich, Jan C; Graver, Hans Petter & Sandlie, Inger (2017). Hvorfor begrunne karakteren? Universitas. ISSN 0805-1798.
-
Frich, Jan C; Graver, Hans Petter & Sandlie, Inger (2017). Slik blir Team Gravers læringsstrategi. Universitas. ISSN 0805-1798.
-
Graver, Hans Petter; Sandlie, Inger & Frich, Jan C (2017). Rektorteam Graver/Sandlie/Frich: Ledelse og administrasjon. Uniforum. ISSN 1891-5825.
-
Frich, Jan C; Graver, Hans Petter & Sandlie, Inger (2017). Slik skal team Graver, Sandlie & Frich gjøre UiO til læringsuniversitetet. Uniforum. ISSN 1891-5825.
-
Sandlie, Inger; Graver, Hans Petter & Frich, Jan C (2017). Mindre midlertidighet. Klassekampen. ISSN 0805-3839. p. 24–24.
-
Mandecki, W.; Goldman, E.; Sandlie, Inger & Løset, Geir Åge (2015). Phage Display and Selection of Protein Ligands. In Goldman, Emanuel & Green, Laurence (Ed.), Practical Handbook of Microbiology, Third Edition. CRC Press. ISSN 9781466587397. p. 101–114.
-
Lau, Corinna; Gunnarsen, Kristin Støen; Høydahl, Lene Støkken; Berntzen, Gøril; Stokes, Christopher R. & Espevik, Terje [Show all 8 contributors for this article] (2012). Recombinant anti-human and anti-porcine CD14 antibodies. Immunobiology. ISSN 0171-2985. 217(11), p. 1197–1198. doi: 10.1016/j.imbio.2012.08.196.
-
Høydahl, Lene Støkken; Neumann, Ralf Stefan; Qiao, Shuo Wang; Sollid, Ludvig Magne; Sandlie, Inger & Løset, Geir Åge (2011). Development of a High Affinity TCR Reagent by Phage Display to Study Antigen Presentation in Celiac Disease. Scandinavian Journal of Immunology. ISSN 0300-9475. 73(4), p. 372–372.
-
Qiao, Shuo Wang; Raki, Melinda; Gunnarsen, Kristin Støen; Løset, Geir Åge; Sandlie, Inger & Sollid, Ludvig Magne (2011). Post-Translational Modification of Gluten Shapes the T Cell Response in Coeliac Disease by Direct Interaction with the T Cell Receptor. Scandinavian Journal of Immunology. ISSN 0300-9475. 73(4), p. 356–356.
-
Gunnarsen, Kristin Støen; Kristinsson, Solveig Gunn; Bogen, Bjarne; Sandlie, Inger & Løset, Geir Åge (2011). Thermostability Engineering of a Soluble Single Chain T Cell Receptor (scTCR) Using Phage Display. Scandinavian Journal of Immunology. ISSN 0300-9475. 73(4), p. 352–352.
-
Foss, Stian; Michaelsen, Terje Einar; Sandlie, Inger & Andersen, Jan Terje (2011). The Influence of Removal of the IgG Fab Arms on Binding to FcRn. Scandinavian Journal of Immunology. ISSN 0300-9475. 73(4), p. 350–351. doi: 10.1111/j.1365-3083.2011.02516.x.
-
Qiao, Shuo Wang; Raki, Melinda; Løset, Geir Åge; Sandlie, Inger & Sollid, Ludvig Magne (2010). TCR Vbeta Bias and Semi-Public T Cell Responses In DQ2-Gliadin-Alpha-II Specific T Cells From Coeliac Disease Patients. Scandinavian Journal of Immunology. ISSN 0300-9475. 71(6), p. 511–+.
-
Gunnarsen, Kristin Støen; Kristinsson, Solveig Gunn; Bogen, Bjarne; Sandlie, Inger & Løset, Geir Åge (2009). Thermostability engineering of a soluble single chain T cell receptor using phage display.
-
Michaelsen, Terje Einar; Emilsen, S; Sandlie, I; Bratlie, Diane Lynn Bryant; Langerud, Beathe Kiland & Ihle, Øistein (2009). Human secretory IgM and serum IgM can activate complement equally well.
-
Andersen, JT; Daba, Muluneh B; Berntzen, G; Michaelsen, Terje Einar & Sandlie, I (2009). Cross-species binding analyses of mouse and human neonatal Fc receptor with their ligands. Implications for evaluations of therapeutic antibodies and albumin fusions.
-
Andersen, Jan Terje & Sandlie, Inger (2011). FcRn regulates the pharmacokinetics of albumin. Sojo University Publishing.