benbo

• Derebe, Mulatu Melese; Yu, QinQin; Munshea, Abaineh; Wubetu, Gizachew Yismaw; Fentaw, Surafel & Kefale, Tesfa Addis [Vis alle 16 forfattere av denne artikkelen] (2026). Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Ethiopia, 2021 to 2023. JAC-Antimicrobial Resistance (JAC-AMR). 8(4), s. 1–9. doi: 10.1093/jacamr/dlag126. Fulltekst i vitenarkiv Vis sammendrag

  Abstract Objectives Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is increasing globally, but some regions remain understudied. This study aimed to investigate the AMR phenotypes and genotypes of N. gonorrhoeae isolates from Bahir Dar, Ethiopia, and their phylogenetic relationship to global isolates. Methods Endocervical and urethral swabs were collected from symptomatic patients at sexually transmitted infection (STI) clinics from September 2021 to April 2023. The identified N. gonorrhoeae isolates (n = 70) were subjected to AMR phenotyping and WGS. We generated a recombination-corrected phylogeny with 724 global representative isolates and used phylodynamics to estimate the introduction times of isolates into Bahir Dar. Results All isolates were resistant to ciprofloxacin and tetracycline but susceptible to ceftriaxone, cefixime, azithromycin, and spectinomycin (100% susceptibility); 67 (95.7%) were resistant to penicillin. WGS identified 14 MLSTs, with ST-1587 (54.3%) the dominant sequence type. AMR determinants correlated with phenotypic findings for all antimicrobials. The phylogenetic analysis suggested at least nine introductions of N. gonorrhoeae into Bahir Dar. The ST-1587 isolates were introduced recently, showing close genetic ties to global isolates sampled in 2018–2019 from Asia, Europe, North America, and Oceania. ST-7827 displayed greater genetic divergence from global isolates. Conclusions This study presents WGS of N. gonorrhoeae isolates from Ethiopia, uncovering diverse sequence types and multidrug resistance. These findings underscore the need for routine genomic surveillance, improved antimicrobial stewardship, and targeted public health interventions to combat the spread of AMR and improve STI management in Ethiopia.

• Børud, Bente; Witsø, Anne; Ngam-Asra, Nadjioroum; Antoinette, Mbailamem Demian; Djimtola, Djimtebaye & Halgue, Haoua Oumar [Vis alle 10 forfattere av denne artikkelen] (2026). Direct whole-genome sequencing of clinical specimens highlights the emergence of Neisseria meningitidis ST-10217 in Chad. International Journal of Infectious Diseases. ISSN 1201-9712. 170, s. 1–8. doi: 10.1016/j.ijid.2026.108916. Fulltekst i vitenarkiv
• Børud, Bente & Koomey, Michael (2024). Sweet complexity: O-linked protein glycosylation in pathogenic Neisseria. Frontiers in Cellular and Infection Microbiology. 14, s. 1–13. doi: 10.3389/fcimb.2024.1407863. Fulltekst i vitenarkiv Vis sammendrag

  The genus Neisseria, which colonizes mucosal surfaces, includes both commensal and pathogenic species that are exclusive to humans. The two pathogenic Neisseria species are closely related but cause quite different diseases, meningococcal sepsis and meningitis (Neisseria meningitidis) and sexually transmitted gonorrhea (Neisseria gonorrhoeae). Although obvious differences in bacterial niches and mechanisms for transmission exists, pathogenic Neisseria have high levels of conservation at the levels of nucleotide sequences, gene content and synteny. Species of Neisseria express broad-spectrum O-linked protein glycosylation where the glycoproteins are largely transmembrane proteins or lipoproteins localized on the cell surface or in the periplasm. There are diverse functions among the identified glycoproteins, for example type IV biogenesis proteins, proteins involved in antimicrobial resistance, as well as surface proteins that have been suggested as vaccine candidates. The most abundant glycoprotein, PilE, is the major subunit of pili which are an important colonization factor. The glycans attached can vary extensively due to phase variation of protein glycosylation (pgl) genes and polymorphic pgl gene content. The exact roles of glycosylation in Neisseria remains to be determined, but increasing evidence suggests that glycan variability can be a strategy to evade the human immune system. In addition, pathogenic and commensal Neisseria appear to have significant glycosylation differences. Here, the current knowledge and implications of protein glycosylation genes, glycan diversity, glycoproteins and immunogenicity in pathogenic Neisseria are summarized and discussed.

• Watle, Sara Viksmoen; Børud, Bente; Laake, Ida; Baranowska-Hustad, Marta Natalia; Bryant-Bratlie, Diane Lynn & Bekkevold, Terese [Vis alle 9 forfattere av denne artikkelen] (2023). Antibodies against Neisseria meningitidis serogroups A, C, W and Y in serum and saliva of Norwegian adolescents. Vaccine. ISSN 0264-410X. 41(44), s. 6529–6537. doi: 10.1016/j.vaccine.2023.08.052. Fulltekst i vitenarkiv Vis sammendrag

  Introduction The incidence of invasive meningococcal disease (IMD) among Norwegian 16–19-year-olds was 1–7/100,000 in the decade before the COVID-19 pandemic, with serogroup Y (MenY) dominance. In contrast to many other European countries, meningococcal vaccines are not part of the national immunisation program (NIP) in Norway. This cross-sectional study aimed to measure the degree of natural immunity against Neisseria meningitidis among adolescents in Norway to evaluate the need for introducing tetravalent meningococcal conjugate vaccine (MCV4) in the NIP. Materials and methods Serum and saliva samples were collected from students in upper and lower secondary schools in Norway in 2018. Samples were analysed for meningococcal capsular polysaccharide (PS)-specific antibodies using a bead-based multiplex immunoassay. PS-specific antibody levels were linked to data on meningococcal carriage, vaccination status and risk factors for carriage (assessed with questionnaire) and analysed by linear regression of log transformed concentrations. A subset of samples from unvaccinated individuals was analysed for serum bactericidal antibodies (SBA). Results A total of 1344 participants, median age 16 years (range 12–24), were included in the study. Overall, 60.9% of the participants were female and 1137 (84.6%) were not vaccinated with MCV4. PS-specific antibody concentrations in serum and saliva were low among unvaccinated individuals for all serogroups and only 6.7–20.0% of the subpopulation with high PS-specific antibodies assessed with SBA had protective levels. Unvaccinated MenY carriers had higher levels of MenY anti-PS IgG in serum and IgA in saliva than those not carrying MenY. Use of Swedish snus was associated with lower anti-PS IgG levels in serum and waterpipe use with lower anti-PS IgG levels in saliva. Conclusion Unvaccinated adolescents in Norway have a low degree of natural immunity against the serogroups of N. meningitidis predominating among cases of IMD in this age group. Therefore, introduction of MCV4 for adolescents in the NIP is recommended.

• Næss, Lisbeth Meyer; Maugesten, Ingunn S.; Caugant, Dominique Andree Yvette; Kassu, Afework; Aseffa, Abraham & Børud, Bente (2023). Genetic, Functional, and Immunogenic Analyses of the O-Linked Protein Glycosylation System in Neisseria meningitidis Serogroup A ST-7 Isolates. Journal of Bacteriology. ISSN 0021-9193. 205(3). doi: 10.1128/jb.00458-22. Fulltekst i vitenarkiv
• Wang, Nelson; Anonsen, Jan Haug; Hadjineophytou, Chris; Reinar, William Brynildsen; Børud, Bente & Vik, Åshild [Vis alle 7 forfattere av denne artikkelen] (2020). Allelic polymorphisms in a glycosyltransferase gene shape glycan repertoire in the O-linked protein glycosylation system of Neisseria. Glycobiology. ISSN 0959-6658. doi: 10.1093/glycob/cwaa073. Fulltekst i vitenarkiv Vis sammendrag

  Glycosylation of multiple proteins via O-linkage is well documented in bacterial species of Neisseria of import to human disease. Recent studies of protein glycosylation (pgl) gene distribution established that related protein glycosylation systems occur throughout the genus including nonpathogenic species. However, there are inconsistencies between pgl gene status and observed glycan structures. One of these relates to the widespread distribution of pglG, encoding a glycosyltransferase that in Neisseria elongata subsp. glycolytica is responsible for the addition of di-N-acetyl glucuronic acid at the third position of a tetrasaccharide. Despite pglG residing in strains of N. gonorrhoeae, N. meningitidis and N. lactamica, no glycan structures have been correlated with its presence in these backgrounds. Moreover, PglG function in N. elongata subsp. glycolytica minimally requires UDP-glucuronic acid (GlcNAcA), and yet N. gonorrhoeae, N. meningitidis and N. lactamica lack pglJ, the gene whose product is essential for UDP-GlcNAcA synthesis. We examined the functionality of pglG alleles from species spanning the Neisseria genus by genetic complementation in N. elongata subsp. glycolytica. The results indicate that select pglG alleles from N. meningitidis and N. lactamica are associated with incorporation of an N-acetyl-hexosamine at the third position and reveal the potential for an expanded glycan repertoire in those species. Similar experiments using pglG from N. gonorrhoeae failed to find any evidence of function suggesting that those alleles are missense pseudogenes. Taken together, the results are emblematic of how allelic polymorphisms can shape bacterial glycosyltransferase function and demonstrate that such alterations may be constrained to distinct phylogenetic lineages.

• Børud, Bente; Bårnes, Guro Kristine; Brynildsrud, Ola Brønstad; Fritzsønn, Elisabeth & Caugant, Dominique A (2018). Genotypic and phenotypic characterization of the O-linked protein glycosylation system reveals high glycan diversity in paired meningococcal carriage isolates. Journal of Bacteriology. ISSN 0021-9193. 200(16). doi: 10.1128/JB.00794-17. Fulltekst i vitenarkiv
• Bårnes, Guro Kristine; Brynildsrud, Ola Brønstad; Børud, Bente; Workalemahu, Bereket; Kristiansen, Paul Arne & Beyene, Demissew Balcha [Vis alle 8 forfattere av denne artikkelen] (2017). Whole genome sequencing reveals within-host genetic changes in paired meningococcal carriage isolates from Ethiopia. BMC Genomics. 18. doi: 10.1186/s12864-017-3806-3. Fulltekst i vitenarkiv Vis sammendrag

  Background Meningococcal colonization is a prerequisite for transmission and disease, but the bacterium only very infrequently causes disease while asymptomatic carriage is common. Carriage is highly dynamic, showing a great variety across time and space within and across populations, but also within individuals. The understanding of genetic changes in the meningococcus during carriage, when the bacteria resides in its natural niche, is important for understanding not only the carriage state, but the dynamics of the entire meningococcal population. Results Paired meningococcal isolates, obtained from 50 asymptomatic carriers about 2 months apart were analyzed with whole genome sequencing (WGS). Phylogenetic analysis revealed that most paired isolates from the same individual were closely related, and the average and median number of allelic differences between paired isolates defined as the same strain was 35. About twice as many differences were seen between isolates from different individuals within the same sequence type (ST). In 8%, different strains were detected at different time points. A difference in ST was observed in 6%, including an individual who was found to carry three different STs over the course of 9 weeks. One individual carried different strains from the same ST. In total, 566 of 1605 cgMLST genes had undergone within-host genetic changes in one or more pairs. The most frequently changed cgMLST gene was relA that was changed in 47% of pairs. Across the whole genome, pilE, differed mostly, in 85% of the pairs. The most frequent mechanisms of genetic difference between paired isolates were phase variation and recombination, including gene conversion. Different STs showed variation with regard to which genes that were most frequently changed, mostly due to absence/presence of phase variation.

• Anonsen, Jan Haug; Børud, Bente; Vik, Åshild; Viburiene, Raimonda & Koomey, Michael (2017). Structural and genetic analyses of glycan O-acetylation in a bacterial protein glycosylation system: evidence for differential effects on glycan chain length. Glycobiology. ISSN 0959-6658. 27(9), s. 888–899. doi: 10.1093/glycob/cwx032. Fulltekst i vitenarkiv
• Anonsen, Jan Haug; Vik, Åshild; Børud, Bente; Viburiene, Raimonda; Aas, Finn Erik & Kidd, Shani Wilhelmina Aarek [Vis alle 8 forfattere av denne artikkelen] (2016). Characterization of a unique tetrasaccharide and distinct glycoproteome in the O-linked protein glycosylation system of Neisseria elongata subsp. glycolytica. Journal of Bacteriology. ISSN 0021-9193. 198(2), s. 256–267. doi: 10.1128/JB.00620-15. Fulltekst i vitenarkiv
• Børud, Bente; Anonsen, Jan Haug; Viburiene, Raimonda; Cohen, Ellen Hanne; Samuelsen, Anne Berit C. & Koomey, Michael (2014). Extended glycan diversity in a bacterial protein glycosylation system linked to allelic polymorphisms and minimal genetic alterations in a glycosyltransferase gene. Molecular Microbiology. ISSN 0950-382X. Volume 94(3), s. 688–699. doi: 10.1111/mmi.12789. Fulltekst i vitenarkiv Vis sammendrag

  Glycans manifest in conjunction with the broad spectrum O-linked protein glycosylation in species within the genus Neisseria display intra- and interstrain diversity. Variability in glycan structure and antigenicity are attributable to differences in the content and expression status of glycan synthesis genes. Given the high degree of standing allelic polymorphisms in these genes, the level of glycan diversity may exceed that currently defined. Here, we identify unique protein-associated disaccharide glycoforms that carry N-acetylglucosamine (GlcNAc) at their non-reducing end. This altered structure was correlated with allelic variants of pglH whose product was previously demonstrated to be responsible for the expression of glucose (Glc)-containing disaccharides. Allele comparisons and site-specific mutagenesis showed that the presence of a single residue, alanine at position 303 in place of a glutamine, was sufficient for GlcNAc versus Glc incorporation. Phylogenetic analyses revealed that GlcNAc-containing disaccharides may be widely distributed within the pgl systems of Neisseria particularly in strains of N. meningitidis. Although analogous minimal structural alterations in glycosyltransferases have been documented in association with lipopolysaccharide and capsular polysaccharide variability, this appears to be the first example in which such changes have been implicated in glycan diversification within a bacterial protein glycosylation system.

• Viburiene, Raimonda; Vik, Åshild; Børud, Bente & Koomey, Michael (2013). Allelic Variation in a Simple Sequence Repeat Element of Neisserial pglB2 and Its Consequences for Protein Expression and Protein Glycosylation. Journal of Bacteriology. ISSN 0021-9193. 195(15), s. 3476–3485. doi: 10.1128/JB.00276-13. Fulltekst i vitenarkiv
• Anonsen, Jan Haug; Jacobsen, Wolfgang Mathias; Aas, Finn Erik; Børud, Bente; Koomey, Michael & Vik, Åshild (2012). Novel protein substrates of the phospho-form modification system in neisseria gonorrhoeae and their connection to O-linked protein glycosylation. Infection and Immunity. ISSN 0019-9567. 80(1), s. 22–30. doi: 10.1128/IAI.05920-11. Fulltekst i vitenarkiv
• Vik, Åshild; Aspholm, Marina; Anonsen, Jan Haug; Børud, Bente; Roos, Norbert & Koomey, Michael (2012). Insights into type IV pilus biogenesis and dynamics from genetic analysis of a C-terminally tagged pilin: a role for O-linked glycosylation. Molecular Microbiology. ISSN 0950-382X. 85(6), s. 1166–1178. doi: 10.1111/j.1365-2958.2012.08166.x. Fulltekst i vitenarkiv
• Johannessen, Camilla; Koomey, Michael & Børud, Bente (2012). Hypomorphic glycosyltransferase alleles and recoding at contingency Loci influence glycan microheterogeneity in the protein glycosylation system of Neisseria species. Journal of Bacteriology. ISSN 0021-9193. 194(18), s. 5034–5043. doi: 10.1128/JB.00950-12. Fulltekst i vitenarkiv
• Børud, Bente; Viburiene, Raimonda; Hartley, Meredith D; Paulsen, Berit Smestad; Jacobsen, Wolfgang Mathias & Imperiali, Barbara [Vis alle 7 forfattere av denne artikkelen] (2011). Genetic and molecular analyses reveal an evolutionary trajectory for glycan synthesis in a bacterial protein glycosylation system. Proceedings of the National Academy of Sciences of the United States of America (PNAS). ISSN 0027-8424. 108(23), s. 9643–9648. doi: 10.1073/pnas.1103321108. Fulltekst i vitenarkiv
• Hartley, Meredith D; Morrison, MJ; Aas, Finn Erik; Børud, Bente; Koomey, Michael & Imperiali, Barbara (2011). Biochemical Characterization of the O-Linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Protein Glycans Containing N,N '-Diacetylbacillosamine. Biochemistry. ISSN 0006-2960. 50(22), s. 4936–4948. doi: 10.1021/bi2003372. Fulltekst i vitenarkiv
• Børud, Bente; Aas, Finn Erik; Vik, Åshild; Winther-Larsen, Hanne Cecilie; Jacobsen, Wolfgang Mathias & Koomey, Michael (2010). Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species. Journal of Bacteriology. ISSN 0021-9193. 192(11), s. 2816–2829. doi: 10.1128/JB.00101-10. Fulltekst i vitenarkiv
• Hoang, Tuyen; Fenne, Ingvild Sveinsgjerd; Cook, Carol Ann; Børud, Bente; Bakke, Marit & Lien, Ernst Asbjørn [Vis alle 7 forfattere av denne artikkelen] (2004). cAMP-dependent protein kinase regulates ubiquitin-proteasome-mediated degradation and subcellular localization of the nuclear receptor coactivator GRIP1. Journal of Biological Chemistry. ISSN 0021-9258. 279(47), s. 49120–49130. Fulltekst i vitenarkiv
• Børud, Bente; Mellgren, Gunnar; Lund, Johan & Bakke, Marit (2003). Cloning and Characterization of a Novel Zinc Finger Protein that Modulates the Transcriptional Activity of Nuclear Receptors. Molecular Endocrinology. ISSN 0888-8809. 17(11), s. 2303–2319. Fulltekst i vitenarkiv
• Mellgren, Gunnar; Børud, Bente; Hoang, Tuyen Thi Van; Yri, Olav Erich; Fladeby, Cathrine & Lien, Ernst Asbjørn [Vis alle 7 forfattere av denne artikkelen] (2003). Characterization of receptor-interacting protein RIP140 in the regulation of SF-1 responsive target genes. Molecular and Cellular Endocrinology. ISSN 0303-7207. 203, s. 91–103. Fulltekst i vitenarkiv
• Børud, Bente; Hoang, Tuyen Thi Van; Bakke, Marit; Jacob, Anne Lilybert J; Lund, Johan & Mellgren, Gunnar (2002). The Nuclear Receptor Coactivators p300/CBP/Cointegrator-Associated Protein (p/CIP) and Transcription Intermediary Factor 2 (TIF2) Differentially Regulate PKA-Stimulated Transcriptional Activity of Steroidogenic Factor 1. Molecular Endocrinology. ISSN 0888-8809. 16(4), s. 757–773. Fulltekst i vitenarkiv
• Lund, Johan; Børud, Bente; Mellgren, Gunnar; Æsøy, Reidun; Hoang, Tuyen Thi Van & Jacob, Anne Lilybert J [Vis alle 7 forfattere av denne artikkelen] (2002). Differential regulation of SF-1-cofactor interactions. Endocrine Research. ISSN 0743-5800. 28(4), s. 505–513. Fulltekst i vitenarkiv
• Lund, Johan; Børud, Bente; Mellgren, Gunnar; Æsøy, Reidun; Hoang, Tuyen Thi Van & Jacob, Anne L. [Vis alle 7 forfattere av denne artikkelen] (2002). Differential regulation of SF-1-cofactor interactions. Endocrine Research. ISSN 0743-5800. doi: 10.1081/erc-120016830. Fulltekst i vitenarkiv

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• Bruun, Tone; Børud, Bente; Finbråten, Ane-Kristine; Grenersen, Marte Petrikke; Kløvstad, Hilde & Olsen, Anne Olaug [Vis alle 7 forfattere av denne artikkelen] (2026). Årsrapport 2025. Blod- og seksuelt overførbare infeksjoner i Norge. Fulltekst i vitenarkiv
• Viburiene, Raimonda; Børud, Bente; Vik, Åshild; Anonsen, Jan Haug & Koomey, Michael (2014). Genetic, structural, and phylogenetic studies of the general O-linked protein glycosylation system in Neisseria. Fulltekst i vitenarkiv
• Viburiene, Raimonda; Børud, Bente; Vik, Åshild; Anonsen, Jan Haug & Koomey, Michael (2014). Genetic, structural, and phylogenetic studies of the general O-linked protein glycosylation system in Neisseria. Fulltekst i vitenarkiv
• Viburiene, Raimonda; Anonsen, Jan Haug; Vik, Åshild; Koomey, Michael & Børud, Bente (2014). Identification and functional characterization of a split ORF variants of the bifunctional PglB and PglB2 glycosylation proteins in Neisseria. Fulltekst i vitenarkiv
• Viburiene, Raimonda; Børud, Bente; Anonsen, Jan Haug; Vik, Åshild & Koomey, Michael (2014). Status and strategies for vaccine prevention of gonorrhoea. Fulltekst i vitenarkiv
• Anonsen, Jan Haug; Børud, Bente & Koomey, Michael (2013). Mass spectrometry based investigation of post-translational modification systems in Neisseria. Fulltekst i vitenarkiv
• Anonsen, Jan Haug; Børud, Bente & Koomey, Michael (2013). Mass spectrometry based investigation of post-translational modification systems in Neisseria. Fulltekst i vitenarkiv
• Anonsen, Jan Haug; Vik, Åshild; Børud, Bente; Aas, Finn Erik; Viburiene, Raimonda & Moen, Anders [Vis alle 7 forfattere av denne artikkelen] (2013). Mass spectrometry based investigation of post-translational modification systems in Neisseria. Fulltekst i vitenarkiv
• Børud, Bente; Viburiene, Raimonda; Johannessen, Camilla & Koomey, Michael (2012). Intrinsic genetic interactions and their role in the evolution ofglycan repertoire and microheterogeneity. Fulltekst i vitenarkiv
• Børud, Bente; Viburiene, Raimonda; Aas, Finn Erik; Vik, Åshild; Hartley, Meredith D & Egge-Jacobsen, Wolfgang [Vis alle 8 forfattere av denne artikkelen] (2011). Neisseria species as a model system for bacterial O-linked protein glycosylation. Fulltekst i vitenarkiv
• Koomey, Michael; Viburiene, Raimonda; Aas, Finn Erik; Vik, Åshild; Anonsen, Jan Haug & Egge-Jacobsen, Wolfgang [Vis alle 7 forfattere av denne artikkelen] (2011). Bacterial O-linked protein glycosylation: the perspective from within the genus Neisseria. Fulltekst i vitenarkiv
• Børud, Bente; Viburiene, Raimonda; Hartley, Meredith D; Paulsen, Berit Smestad; Egge-Jacobsen, Wolfgang & Imperiali, Barbara [Vis alle 7 forfattere av denne artikkelen] (2011). Genetic and molecular analyses reveal an evolutionary trajectory for glycan synthesis in a bacterial protein glycosylation system. Fulltekst i vitenarkiv
• Børud, Bente; Aas, Finn Erik; Vik, Åshild; Egge-Jacobsen, Wolfgang & Koomey, Michael (2009). Glycomic and genetic strategies define glycoprotein carbohydrate diversity in Neisseria gonorrhoeae and related species. Fulltekst i vitenarkiv
• Børud, Bente; Mellgren, Gunnar; Lund, Johan & Bakke, Marit (2002). Cloning and characterization of a novel zinc-finger protein that represses the activity of Steroidognic Factor-1. Fulltekst i vitenarkiv
• Mellgren, Gunnar; Børud, Bente; Æsøy, Reidun; Hoang, Tuyen Thi Van; Bakke, Marit & Lien, Ernst Asbjørn [Vis alle 7 forfattere av denne artikkelen] (2002). Steroidogenesis - Role of Steroidogenic factor-1 and interacting nuclear receptor coregulators. Fulltekst i vitenarkiv
• Børud, Bente; Hoang, Tuyen Thi Van; Bakke, Marit; Jacob, Anne Lilybert J; Lund, Johan & Mellgren, Gunnar (2002). Differential regulation of SF-1 dependent transcriptional activity by p160-coactivators. Fulltekst i vitenarkiv
• Børud, Bente; Mellgren, Gunnar; Jacob, Anne Lilybert J; Bakke, Marit & Lund, Johan (2001). The nuclear receptor coactivators p/CIP and TIF2 that differentially regulate the PKA dependent transcriptional activity of Steroidogenic Factor-1. Fulltekst i vitenarkiv Vis sammendrag

  Steroidogenic factor-1 (SF-1) is a member of the nuclear receptor superfamily and plays an essential role in the development of endocrine organs. Coactivators and corepressors are important in the transcriptional regulation of gene expression by nuclear receptors. SRC-1 and TIF2 belongs to the SRC family of coactivators and are required for transcriptional regulation by several nuclear receptors including SF-1. Here we demonstrate that another SRC-related coactivator, p/CIP, interacts with SF-1, and that the interaction requires the carboxy-terminal activation function-2 (AF-2) domain of SF-1. By transfection studies performed in an adrenocortical cell line (Y1) and nonsteroidogenic COS-1 cells it is shown that both p/CIP and TIF2 potentates SF-1 mediated transactivation of a reporter construct under the control of the SF-1 binding site (CRS2) from the bovine CYP17 gene. Cotransfection with the catalytic subunit of cAMP-dependent protein kinase (PKA) demonstrated that increased PKA activity further stimulated the positive effect of p/CIP. In contrast, PKA markedly inhibited the positive effect of TIF2 on the transcriptional activity of SF-1. The AF-2 domain of SF-1 is required for the coactivating effect of both p/CIP and TIF2. However, the interaction between TIF2 and SF-1 differed from that of p/CIP and SF-1 with respect to the amino acid requirements within the AF-2 domain. Based on these results, we suggest that TIF2 acts by a mechanism distinct from that by which the other members of the SRC family to regulate the transcriptional activity of SF-1.

• Børud, Bente; Lund, Johan & Bakke, Marit (2001). Cloning and characterization of a novel SF-1 interacting protein. Fulltekst i vitenarkiv Vis sammendrag

  Steroidogenic Factor-1 is a member of the orphan nuclear receptor family that acts as a transcription factor to regulate transcription of several genes involved in adrenal and gonadal development and steroidogenesis. A yeast two-hybrid screen with SF-1 as a bait was initiated to search for proteins that interact with SF-1 and further regulate transcription of the SF-1 target genes. An unknown sequence was isolated from a human testis library in this yeast two-hybrid screen. The full-length cDNA was then cloned from a lambda library, and a BLAST search revealed that this gene is localized to the human chromosome 19 and contains 9 exons. The predicted protein contains 615 amino acids and five C2H2 zinc fingers. The interaction with SF-1 is dependent on the activation function-2 domain (AF-2) of SF-1. Northern hybridization and In situ hybridization demonstrated coexpression with SF-1 in the testis and the adrenals. Transfection experiments in adrenal cortical Y1 cells indicate that the possible function of this novel SF-1 interacting protein is repression of the SF-1 target genes Cytochrome P450 steroid hydroxylase-17a (CYP17) and the Cholesterol side chain cleavage cytochrome P450 (CYP11A). ________________________________________

• Mellgren, Gunnar; Børud, Bente; Yri, Olav Erik; Fladeby, Cathrine; Lien, Ernst Asbjørn & Lund, Johan (2001). Receptor Interacting Protein RIP140 inhibits transcriptional activity of Steroidogenic factor-1. Fulltekst i vitenarkiv
• Mellgren, Gunnar; Børud, Bente; Yri, Olav Erik; Fladeby, Cathrine; Lien, Ernst Asbjørn & Lund, Johan (2001). Receptor Interacting Protein RIP140 inhibits transcriptional activity of Steroidogenic factor–1. Fulltekst i vitenarkiv
• Børud, Bente; Mellgren, Gunnar & Lund, Johan (2000). Steroidogenic factor-1 interacts with the coactivator p/CIP (2000). Fulltekst i vitenarkiv
• Børud, Bente; Jacob, Anne Lilybert J; Lund, Johan & Mellgren, Gunnar (2000). Steroidogenic factor-1 interacts with the coactivators p/CIP and TIF2 through different amino acids of the activation function-2 domain. Fulltekst i vitenarkiv
• Simonsen, Gunnar Skov; Blix, Hege Salvesen; Helgesen, Kari Marie Olli; Urdahl, Anne Margrete; Afset, Jan Egil & Andersen, Cecilie Torp [Vis alle 23 forfattere av denne artikkelen] (2026). NORM/NORM-VET 2025 : Usage of Antimicrobial Agents and Occurrence of Antimicrobial Resistance in Norway. Folkehelseinstituttet. Fulltekst i vitenarkiv
• Simonsen, Gunnar Skov; Blix, Hege Salvesen; Helgesen, Kari Marie Olli; Urdahl, Anne Margrete; Afset, Jan Egil & Andersen, Cecilie Torp [Vis alle 23 forfattere av denne artikkelen] (2025). NORM/NORM-VET 2024. Usage of Antimicrobial Agents and Occurrence of Antimicrobial Resistance in Norway. Veterinærinstituttet. doi: https:/www.vetinst.no/overvaking/antibiotikaresistens-norm-vet/_/attachment/inline/78155e88-2b2e-42a1-ac0c-b06071eb0479:21bb52d0f6c051c93f0d4a0605eb001e25e7fe99/NORM%20NORM-VET%202023%20(2).pdf. Fulltekst i vitenarkiv
• Bruun, Tone; Børud, Bente; Caugant, Dominique Andree Yette; Kløvstad, Hilde; Olsen, Anne Olaug & Pires, Joao Pedro Do Couto [Vis alle 8 forfattere av denne artikkelen] (2025). Blod- og seksuelt overførbare infeksjoner. Årsrapport 2024. Folkehelseinstituttet. ISSN 9788284064987. Fulltekst i vitenarkiv
• Børud, Bente (2002). Transcriptional regulation by Steroidogenic Factor-1 and Interacting Coregulators. Universitetet i Bergen. Fulltekst i vitenarkiv

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