Catherine Anne Heyward

• Ganan, M; Lorentzen, Silje Benedicte; Wittrup_Agger, Jane; Heyward, Catherine Anne; Bakke, Oddmund; Knutsen, Svein Halvor; Aam, Berit Bjugan; Eijsink, Vincent; Gaustad, Peter & Sørlie, Morten (2019). Antifungal activity of well-defined chitooligosaccharide preparations against medically relevant yeasts. PLOS ONE.  ISSN 1932-6203.  14(1) . doi: 10.1371/journal.pone.0210208 Fulltekst i vitenarkiv. Vis sammendrag

  Due to their antifungal activity, chitosan and its derivatives have potential to be used for treating yeast infections in humans. However, to be considered for use in human medicine, it is necessary to control and know the chemical composition of the compound, which is not always the case for polymeric chitosans. Here, we analyze the antifungal activity of a soluble and well-defined chito-oligosaccharide (CHOS) with an average polymerization degree (DPn) of 32 and fraction of acetylation (FA) of 0.15 (C32) on 52 medically relevant yeast strains. Minimal inhibitory concentrations (MIC) varied widely among yeast species, strains and isolates (from > 5000 to < 9.77 μg mL-1) and inhibition patterns showed a time- and dose-dependencies. The antifungal activity was predominantly fungicidal and was inversely proportional to the pH, being maximal at pH 4.5, the lowest tested pH. Furthermore, antifungal effects of CHOS fractions with varying average molecular weight indicated that those fractions with an intermediate degree of polymerization, i.e. DP 31 and 54, had the strongest inhibitory effects. Confocal imaging showed that C32 adsorbs to the cell surface, with subsequent cell disruption and accumulation of C32 in the cytoplasm. Thus, C32 has potential to be used as a therapy for fungal infections.

• Chahal, Aman Singh; Schweikle, Manuel; Heyward, Catherine Anne & Tiainen, Hanna (2018). Attachment and spatial organisation of human mesenchymal stem cells on poly(ethylene glycol) hydrogels. Journal of The Mechanical Behavior of Biomedical Materials.  ISSN 1751-6161.  84, s 46- 53 . doi: 10.1016/j.jmbbm.2018.04.025
• Stakkestad, Øystein; Heyward, Catherine Anne; Lyngstadaas, Ståle Petter; Medin, Tirill; Vondrasek, Jiri; Lian, Aina-Mari; Pezeshki, Gita & Reseland, Janne Elin (2018). An ameloblastin C-terminus variant is present in human adipose tissue. Heliyon.  ISSN 2405-8440.  4(12) . doi: 10.1016/j.heliyon.2018.e01075 Fulltekst i vitenarkiv.

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