-
Gutierrez Fernandez, Javier; Hammerstad, Marta & Hersleth, Hans-Petter
(2023).
Structural determination of Mycobacterium smegmatis and Rhodococcus erythropolis mycothiol disulphide reductases.
-
Gutierrez Fernandez, Javier; Hammerstad, Marta & Hersleth, Hans-Petter
(2023).
Structural determination of Mycobacterium smegmatis and Rhodococcus erythropolis mycothiol disulphide reductases.
-
Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2023).
A Research-inspired Laboratory Module on a Redox Protein Combining Biochemistry and Structural Biology.
-
Gutierrez Fernandez, Javier; Hammerstad, Marta & Hersleth, Hans-Petter
(2023).
Structural determination of Mycobacterium smegmatis and Rhodococcus erythropolis mycothiol disulphide reductases.
-
Karlsen, Marthe Lindahl; Hersleth, Hans-Petter & Hammerstad, Marta
(2023).
Diversity among bacillithiol disulfide reductases – a structural and functional investigation.
-
Hammerstad, Marta; Andersen, Hilde Kristin; Rugtveit, Anne Kristine; Dahlen, Sondov Åsmundson Braathen & Hersleth, Hans-Petter
(2023).
Functional diversity among flavin-containing oxidoreductases involved in redox protection, iron homeostasis, and ribonucleotide reduction.
-
Gutierrez Fernandez, Javier; Hammerstad, Marta & Hersleth, Hans-Petter
(2022).
Structural determination of Mycobacterium tuberculosis and Rhodococcus erythropolis mycothiol disulphide reductases.
-
Rese, Morten; Hammerstad, Marta & Hersleth, Hans-Petter
(2022).
Diversity in myoglobin function.
-
Andersen, Hilde Kristin; Hersleth, Hans-Petter & Hammerstad, Marta
(2022).
Structure-function studies of ferredoxin-flavodoxin NADP+ oxidoreductase 1 from Bacillus cereus as an iron-uptake oxidoreductase.
-
Hammerstad, Marta; Gudim, Ingvild & Hersleth, Hans-Petter
(2022).
Redox protection of pathogens by low molecular weight thiols.
-
Rese, Morten; Hammerstad, Marta & Hersleth, Hans-Petter
(2022).
Diversity in myoglobin function.
-
Hammerstad, Marta; Gudim, Ingvild & Hersleth, Hans-Petter
(2022).
Redox protection of pathogens by low molecular weight thiols.
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Hammerstad, Marta; Gudim, Ingvild & Hersleth, Hans-Petter
(2020).
The Crystal Structures of Bacillithiol Disulfide Reductase YpdA Reveal Structural and Functional Insight into a New Type of FAD-Containing NADPH-Dependent Oxidoreductases.
-
-
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Hammerstad, Marta; Gudim, Ingvild; Lofstad, Marie; Røhr, Åsmund Kjendseth & Hersleth, Hans-Petter
(2019).
Enzyme Activation by a Flavoprotein Redox Network.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; van Beek, Wouter & Hersleth, Hans-Petter
(2019).
New structural insight into the well known peptide flip observed in flavodoxins.
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Hammerstad, Marta; Zaltariov, Mirela F.; Arabshahi, Homayon John; Jovanovic, Katarina; Richter, Klaus W. & Cazacu, Maria
[Vis alle 14 forfattere av denne artikkelen]
(2019).
New thiosemicarbazone derivatives and their copper(II) complexes as potential inhibitors against mammalian ribonucleotide reductase.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Røhr, Åsmund Kjendseth & Hersleth, Hans-Petter
(2019).
Activation of the Class Ib Ribonucleotide Reductase by a Flavodoxin Reductase in Bacillus cereus.
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Kendall-Price, Sophie; Evans, Rhiannon M; Rowbotham, Jack S; Reeve, Holly A; Carr, Stephen B & Frogley, Mark D
[Vis alle 11 forfattere av denne artikkelen]
(2019).
Generating Single Metalloprotein Crystals in Well-Defined Redox States – Electrochemical Microspectroscopy As a Tool for Mechanistic Studies.
Meeting Abstracts - The Electrochemical Society [ECS].
ISSN 1091-8213.
9,
s. 425–425.
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Andersen, Niels Højmark; Hammerstad, Marta; Zaltariov, Mirela F.; Rapta, Peter; Arion, Vladimir B. & Hersleth, Hans-Petter
(2019).
Interaction of Thiosemicarbazones with the Ribonucleotide Reductase R2 subunit Studied by Resonance Raman Spectroscopy.
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Hammerstad, Marta; Gudim, Ingvild; Lofstad, Marie; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2019).
Characterization of Proteins in the Ribonucleotide Reductase RedoxNetwork
.
-
Chaudhari, Sujata; Olsbu, Inger Kirstine; Alqarzaee, Abdulelah; Singh, Ryan; Schulze, Thomas & Zimmerman, Matthew
[Vis alle 8 forfattere av denne artikkelen]
(2018).
Endogenous nitric oxide synthase (NOS) activity reduces staphylococcal lifespan during stationary phase.
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Andersen, Niels Højmark; Hammerstad, Marta; Zaltariov, Mirela F.; Arion, Vladimir B. & Hersleth, Hans-Petter
(2018).
Interaction of Thiosemicarbazones with Ribonucleotide Reductase studied by Resonance Raman Spectroscopy.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Røhr, Åsmund Kjendseth & Hersleth, Hans-Petter
(2018).
Activation of the Class Ib Ribonucleotide Reductase by a Flavodoxin Reductase in Bacillus cereus.
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Olsbu, Inger Kirstine; Chaudhari, Sujata; Thomas, Vinai Chittezham & Hersleth, Hans-Petter
(2018).
Potential reductase partner for Staphylococcus aureus Nitric Oxide Synthase.
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Olsbu, Inger Kirstine; Chaudhari, Sujata; Thomas, Vinai Chittezham & Hersleth, Hans-Petter
(2018).
Potential reductase partner for Staphylococcus aureus Nitric Oxide Synthase.
NBS-nytt.
ISSN 0801-3535.
s. 40–40.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta & Hersleth, Hans-Petter
(2018).
Enzyme activation by a flavoprotein redox network.
NBS-nytt.
ISSN 0801-3535.
s. 40–40.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta & Hersleth, Hans-Petter
(2018).
Enzyme activation by a flavoprotein redox network.
-
Johannesen, Hedda; Hammerstad, Marta; Hersleth, Hans-Petter & Andersson, K. Kristoffer
(2017).
A structural and functional investigation of ribonucleotide reductase class III.
-
Johannesen, Hedda; Cumar, Rohit; Hammerstad, Marta; Hersleth, Hans-Petter; Logan, Derek & Andersson, Karl Kristoffer
(2017).
Investigation of NrdD and NrdG from the two Firmicutes Bacillus cereus Lactococcus lactis.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta & Hersleth, Hans-Petter
(2017).
Enzyme activation by a flavoprotein redox network
.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2017).
Activation of the class Ib ribonucleotide reductase by a flavodoxin reductase in Bacillus cereus.
-
Hersleth, Hans-Petter
(2017).
Combining Protein X-ray Crystallography and in situ Single- Crystal UV-Vis and Raman Spectroscopy to Grasp the True Structure of Haem- and Flavoproteins.
-
Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2017).
Activation of the Class Ib Ribonucleotide Reductase by a Flavin Network in Bacillus cereus.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2017).
Activation of the class IB ribonucleotide reductase by a flavodoxin reductase in Bacillus cereus.
-
Hersleth, Hans-Petter
(2017).
Activation of the Class Ib Ribonucleotide Reductase by a Flavin Network in Bacillus cereus.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2017).
Activation of the Class Ib Ribonucleotide Reductase by a Flavin Network in Bacillus cereus.
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Johannesen, Hedda; Kumar, Rohit; Hersleth, Hans-Petter; Hammerstad, Marta; Logan, Derek & Andersson, Karl Kristoffer
(2017).
A STRUCTURAL AND FUNCTIONAL INVESTIGATION OF RIBONUCLEOTIDE REDUCTASE CLASS III IN BACILLUS CEREUS.
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Shoor, Marita; Gudim, Ingvild; Hammerstad, Marta & Hersleth, Hans-Petter
(2017).
Structural and functional characterization of redox proteins in an enzyme activating network involving the thioredoxin reductase in Bacillus cereus.
-
Johannesen, Hedda; Hersleth, Hans-Petter; Hammerstad, Marta & Andersson, K. Kristoffer
(2017).
A structural and functional investigation of ribonucleotide reductase class III in Bacillus cereus.
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Olsbu, Inger Kirstine; Lombard, M; Hersleth, Hans-Petter; Boucher, Jean-Luc & Andersson, K. Kristoffer
(2017).
Key role of Val-567 on L-Arg analogues and heme ligands to neuronal nitric oxide syntase.
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Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2017).
A research-inspired biochemistry laboratory module – Combining, expression,purification, crystallisation, structure solving and characterisation of a flavdoxin-like protein.
NBS-nytt.
ISSN 0801-3535.
s. 63–63.
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Hersleth, Hans-Petter
(2017).
A research-inspired biochemistry laboratory module – Combining, expression,purification, crystallisation, structure solving and characterisation of a flavdoxin-like protein.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2017).
Activation of the class Ib RNR by a flavodoxin reductase in B. cereus.
NSB-nytt.
s. 105–105.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2017).
Activation of the class Ib ribonucleotide reductase by a flavodoxin reductase in Bacillus cereus.
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Hammerstad, Marta; Lofstad, Marie; Böttger, Lars H.; Kjendseth, Åsmund Røhr; Hersleth, Hans-Petter & Zaltariov, Mirela-Fernanda
[Vis alle 9 forfattere av denne artikkelen]
(2017).
INHIBITION OF CLASS 1A RIBONUCLEOTIDE REDUCTASE, AND A COMPARISON OF THE DIMANGANESE ACTIVE SITES OF CLASS IB RIBONUCLEOTIDE REDUCTASE AND MANGANESE CATALASE.
Vis sammendrag
INHIBITION OF CLASS 1A RIBONUCLEOTIDE REDUCTASE, AND A COMPARISON OF THE DIMANGANESE ACTIVE SITES OF CLASS IB RIBONUCLEOTIDE REDUCTASE AND MANGANESE CATALASE
Marta Hammerstad1*; Marie Lofstad1*; Lars H. Böttger2*; Åsmund Kjendseth Røhr3; Hans-Petter Hersleth1; Mirela F. Zaltariov4; Vladimir B. Arion4; Edward I. Solomon2 and K. Kristoffer Andersson1
1Department of Biosciences, University of Oslo, Pb.1066 Blindern, NO-0316 Oslo, Norway
2Department of Chemistry, Stanford University, Stanford, CA 94305, USA
3Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, NO-1432 Ås, Norway
4Institute of Inorganic Chemistry, Universität Wien, AT-1090 Vienna, Austria
*These three authors contributed equally
Presenting Author’s e-mail address: k.k.andersson@ibv.uio.no
Ribonucleotide reductases (RNRs) are enzymes that convert RNA building blocks into DNA building blocks [1]. The reductive reaction by RNRs requires a cysteine thiyl radical, which, in the case of class Ia or Ib RNRs, is initiated by an FeIII2- or MnIII2-tyrosyl radical (Y•) cofactor in the R2 subunit of RNR. During enzymatic turnover, the cofactor is activated by oxygen, and generates a Y• that is transported from the smaller R2 subunit to the large catalytic subunit R1 of RNR, where DNA building blocks are formed.
The small subunit of class Ia RNRs can be inhibited by several small compounds [2], through the inhibition of the active FeIII2- Y• cofactor. We have performed interaction studies and Kd measurements of a mammalian R2 protein with several newly synthesized compounds, and studied their potential inhibitory effect on the protein with EPR, showing promising results.
Manganese catalase (MnCAT) enzymes [3] contain an active site that is similar in structure to the MnIII2 form of NrdF (the R2 subunit in class Ib RNR), characterized by a carboxylate-bridged MnIII-O-MnIII cofactor. However, it catalyzes a different reaction – the degradation of hydrogen peroxide to dioxygen and water. A still unresolved question is how these enzymes containing similar active sites can catalyze different reactions. A variety of spectroscopic methods have been used to try to resolve this question. Samples containing NrdF with active MnIII-O-MnIII cofactor have been prepared and studied by circular dichroism (CD) and magnetic CD (MCD) spectroscopy. The data show both similar and distinct features as compared to MnCAT.
1. A.B. Tomter; G. Zoppelaro; N.H. Andersen; H.-P. Hersleth; M. Hammerstad; Å.K. Røhr; G.K. Sandvik; G.E. Nilsson; C.B. Bell; A.L. Barra; E. Blasco; L. Le Pape; E.I. Solomon; and K.K. Andersson. Coord. Chem. Rev. (2013), 257, 3
2. A. Popovic-Bijelic A; C.R. Kowol; M.E.S. Lind; J. Luo; F. Himo; A.E. Enyedy; V.B. Arion; and A. Gräslund. (2011) J. Inorg. Biochem. 105, 1422-1431
3. T.C. Brunold; D.R. Gamelin; T.L. Stemmler; S.K. Mandal; W.H. Armstrong; J.E. Penner-Hahn; and E. I. Solomon. J. Am. Chem. Soc. (1998), 120, 8724
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2017).
Activation of the class Ib ribonucleotide reductase by a flavodoxin reductase in Bacillus cereus.
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Lofstad, Marie; Gudim, Ingvild; Kjendseth, Åsmund Røhr; Andersen, Niels Højmark; van Beek, Wouter & Andersson, K. Kristoffer
[Vis alle 7 forfattere av denne artikkelen]
(2017).
Combining Protein X-ray crystallography and
in situ single-crystal UV-Vis and Raman spectroscopy
to grasph the true structure of haem- and flavoproteins.
-
Johannesen, Hedda; Hersleth, Hans-Petter; Logan, Derek; Hammerstad, Marta & Andersson, K. Kristoffer
(2016).
A structural and functional investigation of Ribonucleotide reductase Class III in Bacillus cereus- Investigating the interaction and mechanism of the NrdD and NrdG complex.
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Johannesen, Hedda; Hersleth, Hans-Petter; Logan, Derek; Hammerstad, Marta & Andersson, K. Kristoffer
(2016).
A structural and functional investigation of Ribonucleotide reductase Class III in Bacillus cereus- Investigating the interaction and mechanism of the NrdD and NrdG complex
.
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Hersleth, Hans-Petter
(2016).
Probing enzyme activation networks - structural and functional studies of flavoproteins in Bacillus cereus.
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Lofstad, Marie; Böttger, Lars H.; Kjendseth, Åsmund Røhr; Hersleth, Hans-Petter; Hammerstad, Marta & Solomon, Edward I.
[Vis alle 7 forfattere av denne artikkelen]
(2016).
A COMPARISON OF THE DIMANGANESE ACTIVE SITES OF CLASS IB RIBONUCLEOTIDE REDUCTASE AND MANGANESE CATALASE BY CD AND MCD SPECTROSCOPY.
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Gudim, Ingvild; Lofstad, Marie; Hammerstad, Marta; Kjendseth, Åsmund Røhr & Hersleth, Hans-Petter
(2016).
Activation of the Class Ib Ribonucleotide Reductase by a
Flavodoxin Reductase in Bacillus cereus.
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Lofstad, Marie; Gudim, Ingvild; Kjendseth, Åsmund Røhr; Hammerstad, Marta; Andersson, K. Kristoffer & Hersleth, Hans-Petter
(2016).
Activation of Class Ib Ribonucleotide Reductase by NRDI and Its
Reductase Partner in Bacillus cereus.
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Olsbu, Inger Kirstine; Murielle, Lombard; Hersleth, Hans-Petter; Jean-Luc, Boucher & Andersson, K. Kristoffer
(2016).
Key Role of VAL567 on L-Argenine Analogues and HEME Ligands
Binding to Neuronal Nitric Oxide Synthase.
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Johannesen, Hedda; Hersleth, Hans-Petter; Hammerstad, Marta & Andersson, K. Kristoffer
(2016).
A Structural and Functional Investigation of Ribonucleotide
Reductase Class III In Bacillus Cereus.
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Hersleth, Hans-Petter; Gudim, Ingvild; Lofstad, Marie; Kjendseth, Åsmund Røhr; Van beek, Wouter & Giullaume, Pompidor
[Vis alle 11 forfattere av denne artikkelen]
(2016).
Grasping the True Structure of Haem- and Flavoproteins –
Combining X-RAY Crystallography and In Situ Single-Crystal
UV-VIS and Raman Spectroscopy.
-
Hersleth, Hans-Petter
(2016).
Probing enzyme activation networks - structural and functional studies of flavoproteins in Bacillus cereus.
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Lofstad, Marie; Gudim, Ingvild; Van beek, Wouter; Pompidor, Guillaume; Kjendseth, Åsmund Røhr & Andersson, K. Kristoffer
[Vis alle 7 forfattere av denne artikkelen]
(2016).
Radiation damage of haem- and flavoproteins –combining X-ray crystallography and single-crystal UV-Vis and Raman spectroscopy.
9th International Workshop on X-ray Radiation Damage to Biological Crystalline Samples.
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Lofstad, Marie; Gudim, Ingvild; Van beek, Wouter; Pompidor, Guillaume; Kjendseth, Åsmund Røhr & Andersson, K. Kristoffer
[Vis alle 7 forfattere av denne artikkelen]
(2016).
Radiation damage of haem- and flavoproteins – combining X-ray crystallography and single-crystal UV-Vis and Raman spectroscopy.
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Johannesen, Hedda; Hersleth, Hans-Petter; Hammerstad, Marta & Andersson, K. Kristoffer
(2016).
A structural and functional investigation of
Ribonucleotide reductase Class III in Bacillus cereus.
NBS-nytt.
ISSN 0801-3535.
s. 65–65.
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Hammerstad, Marta; Hersleth, Hans-Petter; Tomter, Ane Berg; Røhr, Åsmund Kjendseth & Andersson, K. Kristoffer
(2016).
Structural Insight into the Function of Ribonucleotide
Reductase.
NBS-nytt.
ISSN 0801-3535.
s. 64–64.
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Gudim, Ingvild; Lofstad, Marie; Andersson, K. Kristoffer; Hammerstad, Marta & Hersleth, Hans-Petter
(2016).
Probing enzyme activation networks ‐ structural and functional studies of flavoproteins in Bacillus cereus.
NBS-nytt.
ISSN 0801-3535.
s. 45–45.
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Lofstad, Marie; Böttger, Lars H.; Røhr, Åsmund Kjendseth; Hersleth, Hans-Petter; Hammerstad, Marta & Solomon, Edward I.
[Vis alle 7 forfattere av denne artikkelen]
(2016).
A comparison of the dimanganese active sites of class
Ib ribonucleotide reductase and manganese catalase
by CD and MCD spectroscopy.
NBS-nytt.
ISSN 0801-3535.
s. 45–45.
-
Hammerstad, Marta; Hersleth, Hans-Petter; Lofstad, Marie; Johannesen, Hedda; Tomter, Ane Berg & Røhr, Åsmund Kjendseth
[Vis alle 7 forfattere av denne artikkelen]
(2016).
Structural Insight into the Function of Ribonucleotide Reductase.
-
Hersleth, Hans-Petter
(2016).
Probing enzyme activation networks ‐ structural and
functional studies of flavoproteins in Bacillus cereus.
-
Hersleth, Hans-Petter; Røhr, Åsmund Kjendseth; Van beek, Wouter; Pompidor, Guillaume & Andersson, K. Kristoffer
(2015).
Combining X-ray crystallography and in situ single-crystal UV-Vis and Raman spectroscopy to study redox proteins.
http://www.esrf.eu/files/live/sites/www/files/UsersAndScience/Experiments/CRG/BM01/SNSeminar/Program.pdf.
1,
s. 2–2.
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Hersleth, Hans-Petter
(2015).
Combining X-ray crystallography and in situ single-crystal UV-Vis and Raman spectroscopy to study redox proteins.
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Gudim, Ingvild & Hersleth, Hans-Petter
(2015).
Structures of ferredoxin/flavodoxin-NADP(H) oxidoreductases in Bacillus cereus.
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Lofstad, Marie; Böttger, Lars H.; Røhr, Åsmund Kjendseth; Hammerstad, Marta; Hersleth, Hans-Petter & Solomon, Edward I.
[Vis alle 7 forfattere av denne artikkelen]
(2015).
A comparison of the dimanganese active sites of class Ib ribonucleotide reductase and manganese catalase by CD and MCD spectroscopy.
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Hersleth, Hans-Petter; Røhr, Åsmund Kjendseth; Van beek, Wouter; Pompidor, Guillaume & Andersson, K. Kristoffer
(2015).
Combining X-ray crystallography and in situ single-crystal UV-Vis and Raman spectroscopy to study haem- and flavoproteins.
Acta Crystallographica Section A: Foundations of Crystallography.
ISSN 0108-7673.
A71,
s. s489–s489.
-
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Hersleth, Hans-Petter & Røhr, Åsmund Kjendseth
(2015).
Teaching a general molecular bioscience master course as a project ”From gene to structure”.
.
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Gudim, Ingvild & Hersleth, Hans-Petter
(2015).
Structure of a ferredoxin/flavodoxin-NADP(H) oxidoreductase in Bacillus cereus.
NBS-nytt.
ISSN 0801-3535.
s. 96–96.
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Gudim, Ingvild & Hersleth, Hans-Petter
(2015).
Structure of a ferredoxin/flavodoxin-NADP(H) oxidoreductase in Bacillus cereus.
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Monka, Susanne; Hammerstad, Marta; Andersson, K. Kristoffer & Hersleth, Hans-Petter
(2015).
On the way to determine the structure of a ferredoxin in B. cereus.
Vis sammendrag
Ferredoxins are proteins responsible for the transfer of electrons from NADPH dependent ferredoxin reductases to different enzymes in bacteria that need electrons for activation.
Up to now two genes for ferredoxins have been identified in B. cereus: the bcBC 2795 gene is assumed to code for a ferredoxin with a 2Fe-2S cluster as co-factor and BCbc 1483 for a 4Fe-4S cluster carrying ferredoxin.
The 2Fe-2S ferredoxin gene was cloned into a pET-22b vector and overexpressed in E. coli. The purification procedure involved ammonium sulphate precipitation, ion exchange chromatography using Q Sepharose or DEAE columns, and size-exclusion chromatography with a Superdex 75 column.
The protein detection with UV-Vis spectroscopy (A280 nm) presented a challenge due to the absence of tryptophan and tyrosine residues. Therefore, the protein concentration was determined by Bradford assays.
The faint yellowish colour of the purified protein (106 amino acids, 11.4 kDa) indicated the presence of an apoprotein. The crystallization screening with JCSG+ Suite resulted in several hits, of which X-ray diffraction data was collected to 2.3 Å.
Solving the structure by molecular replacement has so far been unsuccessful, possibly due to a large unit cell of 220 x 220 x 220 Å3.
Further studies are going to involve the reconstitution of iron-sulfur clusters, further characterization and screening for new crystallization conditions.
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Wu, Bernt; Hammerstad, Marta; Andersson, K. Kristoffer & Hersleth, Hans-Petter
(2015).
Structural and functional characterization of flavohemoglobin from Bacillus Cereus.
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Røhr, Åsmund Kjendseth; Hersleth, Hans-Petter; Van Beek, Wouter; Diadkin, Vadim; Wiker, Geir & Chernyshov, Dmitry
[Vis alle 7 forfattere av denne artikkelen]
(2015).
Combining Protein X-Ray Crystallography and Single-Crystal Spectroscopy - A New in situ Setup at BM01A at ESRF.
Vis sammendrag
1Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, Norway
2Swiss-Norwegian Beam Lines, European Synchrotron Radiation Facility, Grenoble, France
E-mail: h.p.hersleth@ibv.uio.no
Redox proteins are essential for all organisms, with functions ranging from substrate oxidation to respiration and photosynthesis. Central to many of these proteins are redox active cofactor as haems, iron-sulfur clusters, flavins, disulfides, quionens or NADPH.
To be able to understand the structure-function relation of these proteins, structural studies are performed with X-ray diffraction. However, for redox proteins the crystal structures are missing key information as oxidation state, protonation or spin state. These informations can be essential for understanding the reaction mechanisms of these proteins. Therefore, a combination of X-ray diffraction and spectroscopic methods like UV-vis and Raman spectroscopy is vital to obtain a deeper understanding of these redox proteins. Additionally, the redox sites are very labile for X-ray induced radiation damage and reduction during crystallographic data collection at synchrotrons.
To determine the redox state of the crystals as well as monitoring potential radiation damage, we have combined the X-ray diffraction setup at the Swiss-Norwegian Beam Lines BM01A (ESRF) with an in situ setup for measuring UV-vis and Raman spectroscopy. The centering and optimisation are performed manually, but the running of the combined setup performing alternating X-ray diffraction experiments and UV-vis and Raman spectroscopy can be programmed and performed in an automatic way.
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Andersen, Hilde Kristin; Hammerstad, Marta & Hersleth, Hans-Petter
(2022).
Struktur-funksjonsstudier av ferredoksin-flavodoksin NADP+ oksidoreduktase 1 fra Bacillus cereus som en jern-opptak oksidoreduktase.
Universitetet i Oslo.
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Rese, Morten; Hersleth, Hans-Petter; Hammerstad, Marta & Andersen, Niels Højmark
(2022).
Functional diversity of myoglobin isozymes from Carassius auratus - Biophysical study of myoglobin as a peroxidase.
Universitetet i Oslo.
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Hersleth, Hans-Petter; Hammerstad, Marta & Dahlen, Sondov Åsmundson Braathen
(2021).
Structure and function of FNR1 from Bacillus cereus undergoing a mutation.
Universitetet i Oslo.
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Rugtveit, Anne Kristine; Hammerstad, Marta & Hersleth, Hans-Petter
(2021).
Structural and functional studies of a ferredoxin/flavodoxin NADP+-oxidoreductase mutant from Bacillus cereus.
Universitetet i Oslo.
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Olsbu, Inger Kirstine; Hersleth, Hans-Petter & Sørlie, Morten
(2018).
Substrate recognition and redox partner identification in nitric oxide synthases.
Reprosentralen, University of Oslo.
ISSN 1501-7710.
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Gudim, Ingvild; Hersleth, Hans-Petter; Hammerstad, Marta & Sørlie, Morten
(2018).
Characterisation of flavodoxin and ferredoxin/flavodoxin reductases from Bacillus cereus and their interactions.
Reprosentralen, University of Oslo.
ISSN 1501-7710.
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Shoor, Marita; Hersleth, Hans-Petter; Gudim, Ingvild & Hammerstad, Marta
(2017).
Structural and functional characterization of the redox protein Thioredoxin reductase from Bacillus cereus.
Universitetet i Oslo.
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Lofstad, Marie; Andersson, K. Kristoffer; Hersleth, Hans-Petter; Hammerstad, Marta & Kjendseth, Åsmund Røhr
(2017).
Activation Pathways of the Class Ib Ribonucleotide Reductase in Bacillus cereus.
Universitet i Oslo.
ISSN 1501-7710.
-
Monka, Susanne; Andersson, K. Kristoffer; Hersleth, Hans-Petter & Hammerstad, Marta
(2015).
Structural and functional characterisation of ferredoxins in Bacillus cereus.
Universitetet i Oslo.
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Purification and characterization of Flavohemoglobin
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Universitetet i Oslo.